Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 3,766,256 C→T 89.7% intergenic (+64/+499) EK12AG100_RS18690 → / ← EK12AG100_RS18695 hypothetical protein/HlyD family secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324043,766,2560CT89.7% 176.7 / 9.2 58intergenic (+64/+499)EK12AG100_RS18690/EK12AG100_RS18695hypothetical protein/HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base T (21/31);  total (23/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATCATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGG  >  NZ_LN832404/3765965‑3766546
                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                  
ggACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATCAt                                                                                                                                                                                                                                                                                                  >  1:406391/1‑294 (MQ=255)
  aCTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCAAATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATCATgg                                                                                                                                                                                                                                                                                                <  2:482408/294‑1 (MQ=255)
    tGGCATATATGGCTGCGAATGATAACAAGGAATTGCGCACATCCTTATCGAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGcc                                                                                                                                                                                                                                                                                              <  2:49465/294‑1 (MQ=255)
              ggCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATTATAAAAATATAAAATTGAGTGTTAATAGTACTATTATGGACATTGTTTAtt                                                                                                                                                                                                                                                                                    >  2:85674/1‑294 (MQ=255)
              ggCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGAtt                                                                                                                                                                                                                                                                                    <  1:360109/294‑1 (MQ=255)
                cTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGAATCt                                                                                                                                                                                                                                                                                  >  2:264186/1‑294 (MQ=255)
                               aGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGat                                                                                                                                                                                                                                                                   <  1:824919/294‑1 (MQ=255)
                               aGGAAGTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGat                                                                                                                                                                                                                                                                   <  1:968161/294‑1 (MQ=255)
                                ggAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGatg                                                                                                                                                                                                                                                                  >  2:704255/1‑294 (MQ=255)
                                         cacaTCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAAc                                                                                                                                                                                                                                                         <  1:390746/294‑1 (MQ=255)
                                           caTCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACtt                                                                                                                                                                                                                                                       <  1:811904/294‑1 (MQ=255)
                                               cTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATCATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAAtt                                                                                                                                                                                                                                                   <  1:139944/294‑1 (MQ=255)
                                                 tATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATAGCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAAtttt                                                                                                                                                                                                                                                 <  1:713110/294‑1 (MQ=255)
                                                       atatAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATCATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTaa                                                                                                                                                                                                                                           <  2:47260/294‑1 (MQ=255)
                                                         ataATGAGGTACAAAATAGATGCTGGACGTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACGCCAACTGGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTaaaa                                                                                                                                                                                                                                         <  1:634700/294‑1 (MQ=255)
                                                           aaTGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGGATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAat                                                                                                                                                                                                                                       >  1:210178/1‑294 (MQ=255)
                                                               aGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATCATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAatatat                                                                                                                                                                                                                                   <  1:233596/294‑1 (MQ=255)
                                                                       aaTAGATGCTGGACTTTCCGAATCCTATACATGCTATTTAGTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAact                                                                                                                                                                                                                           <  2:393817/294‑1 (MQ=255)
                                                                          aGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAactcac                                                                                                                                                                                                                        <  1:340378/294‑1 (MQ=255)
                                                                           gATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAactcact                                                                                                                                                                                                                       <  1:984665/294‑1 (MQ=255)
                                                                                    cTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCAAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAg                                                                                                                                                                                                              <  1:101528/294‑1 (MQ=255)
                                                                                                      tGCTAATTATTAAGTAAAGGAAAGATCATAAGACCATAACTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGtttt                                                                                                                                                                                            <  2:587716/294‑1 (MQ=255)
                                                                                                          atttattAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATaa                                                                                                                                                                                        <  1:265905/294‑1 (MQ=255)
                                                                                                                        ggAAAGATCATAAGACCATATCTGAAAAATCTAAATCCAATCCAACTTACCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAAtt                                                                                                                                                                          <  1:211272/294‑1 (MQ=255)
                                                                                                                               tCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTg                                                                                                                                                                   >  1:891272/1‑294 (MQ=255)
                                                                                                                                cATAAAACCAAAACTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTga                                                                                                                                                                  <  2:415548/294‑1 (MQ=255)
                                                                                                                                         atatCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGaaaa                                                                                                                                                         >  1:287914/1‑294 (MQ=255)
                                                                                                                                          tatCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAAt                                                                                                                                                        <  1:704255/294‑1 (MQ=255)
                                                                                                                                           atCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATa                                                                                                                                                       <  1:1014538/294‑1 (MQ=255)
                                                                                                                                              tGAAAAATCTAAAACCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGtt                                                                                                                                                    <  2:273724/294‑1 (MQ=255)
                                                                                                                                                  aaaTCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGtat                                                                                                                                                <  1:85674/294‑1 (MQ=255)
                                                                                                                                                   aaTCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGtata                                                                                                                                               >  2:78502/1‑294 (MQ=255)
                                                                                                                                                         aaTCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACatata                                                                                                                                         >  1:229658/1‑294 (MQ=255)
                                                                                                                                                            ccACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTc                                                                                                                                      <  1:427338/294‑1 (MQ=255)
                                                                                                                                                              aCTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCa                                                                                                                                    >  2:340581/1‑294 (MQ=255)
                                                                                                                                                                                 tGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCt                                                                                                                 <  2:569854/294‑1 (MQ=255)
                                                                                                                                                                                   cATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGATTACGATAATTAAAGTTGAAGAAGAAAATAGTTGTATACTTATATTCCATGACCCTCCATTGTAATCtat                                                                                                               >  2:587023/1‑294 (MQ=255)
                                                                                                                                                                                     ttGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAg                                                                                                             <  1:633604/294‑1 (MQ=255)
                                                                                                                                                                                         aaCAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTa                                                                                                         >  1:14385/1‑294 (MQ=255)
                                                                                                                                                                                          aCAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTaa                                                                                                        >  1:952498/1‑294 (MQ=255)
                                                                                                                                                                                                aataaaataaAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTACATAttt                                                                                                  >  1:764441/1‑294 (MQ=255)
                                                                                                                                                                                                         aaaGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATCATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAAtgt                                                                                         >  1:589436/1‑294 (MQ=255)
                                                                                                                                                                                                          aaGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAGCTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAAtgtg                                                                                        <  1:985569/294‑1 (MQ=255)
                                                                                                                                                                                                            gATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTATGATCTATAGATAAAATATTTATTAAATGTGAc                                                                                      >  2:691993/1‑294 (MQ=255)
                                                                                                                                                                                                             aTAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACt                                                                                     >  1:1020501/1‑294 (MQ=255)
                                                                                                                                                                                                                                  gATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAg                                                                >  1:7588/1‑294 (MQ=255)
                                                                                                                                                                                                                                    tataAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGcc                                                              <  1:736101/294‑1 (MQ=255)
                                                                                                                                                                                                                                       aaaaTCAGTTAATATTTGTAGTGATGATAAAAATATAAAAATGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCcatc                                                           <  2:169506/294‑1 (MQ=255)
                                                                                                                                                                                                                                       aaaTTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCcatc                                                           <  1:65191/294‑1 (MQ=255)
                                                                                                                                                                                                                                         aTTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCcatcat                                                         >  1:40530/1‑294 (MQ=255)
                                                                                                                                                                                                                                            aaGTGAAGATTTGTAGTGATGATAAAAATATAAAATTGATAGTTAATAGTACTATTATGGCCATTTATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGc                                                      <  2:1066930/293‑1 (MQ=255)
                                                                                                                                                                                                                                                   gatTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGAt                                               <  1:687972/293‑1 (MQ=255)
                                                                                                                                                                                                                                                      ttGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAAGAGATGGa                                            <  1:632460/294‑1 (MQ=255)
                                                                                                                                                                                                                                                              atgatAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACt                                    <  1:476136/294‑1 (MQ=255)
                                                                                                                                                                                                                                                              atgatAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCAATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACt                                    >  1:1057942/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                         ataAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCtttt                         >  2:650654/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                   gagTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTagag               >  2:241819/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                         aTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCg         >  2:675926/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                            gTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCag      >  1:506320/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                                tATGATGTCCATTGATGATTCTAGAAAGTGCATCGATGAATGATAACGTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCaggagg  <  2:433026/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                  
GGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATCATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGG  >  NZ_LN832404/3765965‑3766546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: