| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_LN832404 | 4,168,395 | C→A | 37.1% | intergenic (‑68/‑135) | EK12AG100_RS25840 ← / → EK12AG100_RS20635 | paraslipin/UDP‑N‑acetylmuramate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_LN832404 | 4,168,395 | 0 | C | A | 37.1% | 110.2 / 144.7 | 132 | intergenic (‑68/‑135) | EK12AG100_RS25840/EK12AG100_RS20635 | paraslipin/UDP‑N‑acetylmuramate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (40/43); new base A (20/29); total (60/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 142 total aligned reads displayed.
CCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTC‑TTATTCTGGGTGAAGGAAGTAATGTAC > NZ_LN832404/4168112‑4168686 | ccTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGCTTTGAACGTTGCGAAGCCACGGCCCGGAGGGCGGCGGGGAGGACGACCGCCAAACACTGCACGGCACCACAttca > 2:925343/1‑292 (MQ=11) tGGCGGCAGTAGCGCGGGGGTCCCACCTAACCCGATGCGGAACTAAGAAGGGAAACGCGGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGc < 1:473781/294‑1 (MQ=12) ggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACCGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGaa > 1:166934/1‑294 (MQ=17) gcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCAGGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAg < 1:778915/294‑1 (MQ=17) tAGCGCGGTGGTCCCACCGGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCa < 1:501572/294‑1 (MQ=17) aggcggggggccccACCTGAACCCAGGCCGAACTCGGAAGGGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTATCCCCAGGCGAGAGTAGGGAACGGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCGGTGGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCAt < 2:340296/283‑1 (MQ=11) cgGTGGCCCCACCGGACCCCAGGCCGAACCCGAAAGGGAAACGCCGGAGTGCCGTTGGTAGTGTGGGTTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGTTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTg < 2:279005/294‑1 (MQ=11) ggtggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGAGACGCCCGCCAGCAACTGCCAGGCATCAAGTGAAGCAGAAGGCCATcccgc > 2:491221/1‑291 (MQ=12) gtggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGAc < 1:1052378/294‑1 (MQ=32) cTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCtt < 1:531542/294‑1 (MQ=32) tGACCCCAGGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGTTAGGGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCttt < 1:682778/294‑1 (MQ=17) gCCGAACGCAGAAGTGAAACGCCGGAGCGCCGATGGGAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTc < 2:410819/294‑1 (MQ=18) tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTTTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAAc < 1:419902/294‑1 (MQ=37) aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCATGAGT‑CAGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGAAACGGACCGTAGAGTGGCGGGCCGGATGCCCGCCATAAACTACCAGGCATCAAAATAAGCCAACAGCAAGCCTGACGGATGGCCTTTATACGTTTCTACCCActcat > 2:728169/1‑292 (MQ=11) gTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGACCGTTGCGAAGCAACGGCCCGGAGGGCGGCGGGGAGGACGCCCGCCATAAACTGCCAGGCATCCAATTACGCAGAAGCCCACCCTGACAGATGGCCTTTGTGCGGTTCTACCAACTCTTcc > 2:60705/1‑294 (MQ=11) tGAAGCGCCGGTGCGCCGATTGGAGTGTGTGGTCTACCCCTGTGGGAGAGGGTAACTGCCAGTCTGAAGATAAAATGAAAGGCTCATGCGAAATGT‑TTGGGCGTTCGTTGTATCTGTTGTTTGTCGGTGAACTCTCGCCTGTGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGACGCAACGGCCCGGAGGGGGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCt < 2:949814/294‑1 (MQ=14) gAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTATTCCTg > 1:542788/1‑294 (MQ=38) gCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTgtcgtc > 1:52640/1‑294 (MQ=255) ccGATGGTAGTGTGGGGTCTCCCCATGCGGGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTTTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACa < 1:20666/294‑1 (MQ=255) ggtatgtgggggGCTCCCCAGGCGAGGGTGGGGAACTGCAAGGCAACAAAAAAAACGAAGTGCTCAGTCGAAAGAG‑TGGGCCTTGCGGTTTATCTGTTGTGTGTCGGTGAACGCTCTCCGGAGT‑AGGAAAAATCCGCCGGGAGGGGATTTGAACGTTGGGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCa < 2:93456/286‑1 (MQ=14) gtgGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTTTCTTCctctctcccctccctccccc < 1:226462/294‑15 (MQ=255) tgGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATAAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATcccccc < 1:589634/294‑1 (MQ=255) agggggtggTAGGGAACTGCCAGGCAACAAATAAAACGAAGGGCTCTGTCGAAAGCC‑GGGGCTTTTCGTGTTAGCTGTTGTTTGTCGGGGAACGCTCTCC‑GAGTGAGGACAAATCCGCCGGGGGCGGATTTGAACTTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTa < 2:180413/286‑1 (MQ=17) aTGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTTCGTTTCTCCAAACTCTTCCTGTCGTCATATCTACAAGCCCTCCCCCCACAGATACGGTa > 1:750014/1‑294 (MQ=255) gCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGCTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCCCAGATACGGTaaa > 1:861627/1‑294 (MQ=255) gagTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAg > 1:524055/1‑294 (MQ=255) aggaattaattccccaggcatcaaataaAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatg > 1:1059375‑M2/14‑189 (MQ=255) agTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACACTCTTCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGc < 1:328896/294‑1 (MQ=255) ttaattccccaggcatcaAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgct > 1:339244‑M2/9‑184 (MQ=255) gggAACGGGCAGGAATCAAGTAAACCAAAAGGCT‑AGTGGCAAGGCAGGGGCCTTTGTGTTTTTCGGTGGTTGGTGGGTGAAGGCTCGCCGAAGT‑AGGACAAATCCGCGGGGGGCGGAGTGGAACGTTGCGAAGCAACGGCCCGGAGGGTGGGGGGAAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCg < 2:643918/294‑1 (MQ=17) gCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTAAAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGc > 1:1039774/1‑294 (MQ=255) ggCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACACCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCCTATCTACAAGCCATCCCCCCACAGATACAGTAAACTAGCCTCGTTTTTGCAACa > 1:242350/1‑294 (MQ=255) gCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTCGCCTCGTTTTTGCATCAg > 1:201794/1‑294 (MQ=255) cATCAAATAAAACGAAAGGCTCAGGCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTGGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaac > 1:498702‑M2/1‑171 (MQ=255) aaTAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCACATTAAGCAGCAGGACATCCTGACGGATGGCCTTTTTGCGTTTATACAAACTCTTCCTGTCGTCATATCTACAAACCATCACCACAAAGATACGGTAAACTAGACTCGGTTTTGccaccagaaagc > 2:1048769/1‑283 (MQ=17) aaTAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagttcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttg < 1:334393‑M2/294‑129 (MQ=255) aTAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGCACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGACTtggggatcggaattctcatgtttgacagcttatcatcggggctcttgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccccaaggtaaacagttga < 1:35061‑M2/294‑130 (MQ=255) aaaCGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCCTAAAATGCCAGGAATtggggaacggaattcacaggtttgacagcttatcctcggagctcctgcactggatggtgaccatgggtggtaagccgccggtcagcggtgaacgtcctctggatgtcgccccacaagggaaaccatggcatg < 2:1044466‑M2/294‑133 (MQ=255) ttaaggCTCAGGCTAAAAGC‑TGGGTCTTTCGTTTTATCTGTTGTGTGTCGGGGAACCCTCGCCGGAGT‑AGGACAAAGCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGGGGGGGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaact > 2:252641‑M2/3‑158 (MQ=255) aaaGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactg < 1:276209‑M2/294‑138 (MQ=255) aaGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAAc < 1:444041/294‑1 (MQ=255) aaGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactag < 1:252641‑M2/294‑139 (MQ=255) aaaGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcttgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgc < 1:834611‑M2/294‑151 (MQ=255) aGTC‑TGGTCCTTTCGTGTTAGCTGTTTGTGGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCTCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaatagttgattgaactgcctgaactaccgcag > 1:812518‑M2/1‑142 (MQ=255) aGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgcactgcctgaactaacgcag < 1:1056570‑M2/294‑153 (MQ=255) tttCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGTGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGCGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatactctgatctccggcactggatggtggcgctgggtggtaagccatcggcaagcggtgacgtgactctggatgtcgccaccaagggtcaacagctagttgaaatgcctgaactccccgagccggatagga < 2:327315‑M2/294‑163 (MQ=255) tCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgcc < 1:418344‑M2/294‑165 (MQ=255) tCGTGTTATCTGTTGTTGGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgcc > 1:71150‑M2/1‑130 (MQ=255) gTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATGTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgg > 1:112397‑M2/1‑128 (MQ=255) tttATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggc < 1:279498‑M2/294‑169 (MQ=255) cTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGa < 1:491221/294‑1 (MQ=255) tgtgttggtggTGAACGCTCTCCTGGGT‑AGGAAAACGGCGCCGGGAGCGGATTTGAAGTTGGCGAAGCAACGGCCCGGGGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCa < 2:949281/285‑1 (MQ=25) tttgtCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgactctggatgtcgctccacaaggtacacagttgattgcactgcctgaactaccgcagccggagagcgccgggcaactctgtcct < 1:869535‑M2/294‑180 (MQ=255) ttgtCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAAt > 1:161952/1‑294 (MQ=255) gTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGTTATGAAACACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATACTGCTAAg > 1:598270/1‑294 (MQ=255) tGAACGCGCTCCGTGGG‑AGGACAAACCCGCGGGGAGCGGAGTGGACCGTGGGGAGGCAACGGCCCGGGGGGGGGGGGGCAGGAGGCCCGCCATAAACGGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacg > 2:421158‑M2/1‑107 (MQ=255) gAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccaccaggtaaacagttgattgaactgcctgaactaccccagccggagagcgccgggcaaccctggcccacagtccgc < 2:157506‑M2/294‑189 (MQ=255) aaCGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGcac > 1:819768/1‑294 (MQ=255) gCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACAtt < 1:812305/294‑1 (MQ=255) gCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtag < 1:1034900‑M2/294‑193 (MQ=255) ctcCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTa > 1:249905/1‑294 (MQ=255) ctcCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacacggtaaacagttgattgaactgcctgaactaccccagccggaaagcgccgggcacctctggctcccagtccgcgtaggtc < 2:223530‑M2/294‑196 (MQ=255) ctcCGGGGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgc > 1:1069426‑M2/1‑99 (MQ=255) cTGAGT‑AGGACATATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaac < 1:667094‑M2/294‑199 (MQ=255) gAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccg < 1:739335‑M2/294‑201 (MQ=255) aGT‑AGGAGAAAACCGCAGTGAGCGGATTTGAACGTTGCGAAGAAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTGTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGcc < 1:725506/294‑1 (MQ=255) aGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTATTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCCCGTTTTTGCCTCAGGACAGCACCTATGAACCACTCCTTAAAACCCTGGAACACCTTTGGCCTTGAACATAATTCTCAGccacatctcatgtcc > 2:1044616/1‑280 (MQ=25) gT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCCCGTTTTTGCATCAGGAAAGCAGCGATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATCATTCTCAGCACATTGTATGTGCCg > 1:574442/1‑294 (MQ=255) gACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAACACCCTGGACCACATTTGGCATTGATCATAATGCTCCACACATTGTATGTTCCGacaa > 2:750938/1‑291 (MQ=255) aaaTCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgacc > 1:651784‑M2/1‑85 (MQ=255) aaTCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGaa > 1:91814/1‑294 (MQ=255) gggAGGGGATTGGAAGGTTGCGAAGCAACGGCCCGGAGGGGGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAAtt < 2:767818/294‑1 (MQ=255) gggAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactcacgcagccggagagcgccgggcaaccctggcccccagtacgcgtactgccaccgcacgcgcccgcccggaca < 2:64997‑M2/294‑219 (MQ=255) aTTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGACAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTCTGTCCCGAAGACGAACAAAAATTACCCAATg > 2:55096/1‑294 (MQ=255) tGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCt < 1:711925/294‑1 (MQ=255) ggatggtgcgatgCAGCGGCCTGGAGGGTGGGGAGCAGGCCGTCCGCCATAAACTGCCAGGAATtggggatcggatttctcaggtttggcagcttatcatcggggctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcac > 2:526585‑M2/7‑64 (MQ=255) aaCGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTgg < 1:232606/294‑1 (MQ=255) aaCGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGAAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTgg < 1:925343/294‑1 (MQ=255) cGTTGCGAAGCAACGGCCCGGAGGGGGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCa < 1:206133/294‑1 (MQ=255) gAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGc > 1:1065828/1‑294 (MQ=255) gAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcctggtcagaagccgggcacctcagcgca < 1:141893‑M2/294‑240 (MQ=255) ccAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAAc < 1:856914/293‑1 (MQ=255) cAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAAcc < 1:669227/294‑1 (MQ=255) cGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAAGCGCa > 1:957187/1‑294 (MQ=255) gggTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgtc < 1:75316‑M2/294‑256 (MQ=255) cGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGtt > 1:784458/1‑294 (MQ=255) gggAAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTc < 1:474563/294‑1 (MQ=255) aGGACGCCCGCCATAAACTGCCAGGCATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctgcatcccagtccgcgtagtgcaaccgacagcgacagcatggtcacaagccgggcccctcagcgaccgacagacgcggagtctggccggcacc < 2:650411‑M2/294‑267 (MQ=255) aGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTC‑TTAt > 1:300428/1‑294 (MQ=255) ggcgccccgcctaaaaGTGCAAGGATTgggggagcggaatgttcatgtttgacggcttatcagcgggcgtcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacc > 2:291679‑M2/6‑27 (MQ=255) ggACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTC‑TTAtt > 1:440514/1‑294 (MQ=255) gcccgccATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGACGGACACCCCGTTC‑TTCTTCTgg > 2:777812/1‑294 (MQ=255) gcatgcaaCGGCCAGGCTGCACACTAAGAATAAGTCAATCCTGACGGTTGGCCTGTTGGCGTTTCTACGACCTCTGCCTGTCGTCAGATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTC‑TTATTCTGGGTga < 2:566146/288‑1 (MQ=255) aTAACGTGCCGGGGATggggtatgggaattgttttgtttgtctgcgtatcatcggggctcgtgccctggatggtggcgctggatggtaagccgcgggcgagcggtgaagtgcctcgggatgtcgctccacaaggtaaccagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacg > 2:86401‑M2/1‑16 (MQ=255) aTAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATTCTCAGCACATTGTATGTGCCGAAGAAGAACAACCATTACTCACTGCCTTGCAGTAAGCAAACGGCGGATGACAAACCGGTCATAATTCTggtgacgg > 2:27183/1‑288 (MQ=255) tAAACTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctccaagtactcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacacaagcgcccggcagcagtggcgcctggacgaaaaccccacggttacgc < 2:669545‑M2/294‑280 (MQ=255) aCTGCCAGGAATtggggatcggaattctcatgtttgacagcttatcatcggagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacgctcc < 1:949756‑M2/294‑283 (MQ=255) cTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACACCCCGTTC‑TTATTCTGGGTGAAGGAAGTa > 2:701142/1‑294 (MQ=255) cAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTC‑TTATTCTGGGTGAAGGAAGTAATGt > 1:1014709/1‑294 (MQ=255) ggCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTC‑TTATTCTGGGTGAAGGAAGTAATGTAc < 1:940857/294‑1 (MQ=255) | CCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGAC‑TGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGT‑AGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTC‑TTATTCTGGGTGAAGGAAGTAATGTAC > NZ_LN832404/4168112‑4168686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |