BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	576,961	A→G	47.7%	R47R (AGA→AGG)	EK12AG100_RS02870 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	576,961	0	A	G	47.7%	54.6 / inf	275	R47R (AGA→AGG)	EK12AG100_RS02870	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (68/74); new base G (65/66); total (135/140)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

TTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCA  >  NZ_LN832404/576668‑577254

 tggtgtcGTAACAAAGTGCGGTCCTGCTGGCATTCTGGGGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCagag                                                                                                                                                                                                                                                                                                         <  1:41273/288‑1 (MQ=255)

  ggTTGCGTAACAAAGTGCGGTCCTTCTGGCATTCTGGAGTGAAATACAACCTACAGATGTATGTAATGCCAACGTGCTCAAATCGTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGc                                                                                                                                                                                                                                                                                                        <  2:877099/294‑1 (MQ=255)

   gTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCa                                                                                                                                                                                                                                                                                                       <  1:585195/294‑1 (MQ=255)

     tGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGACCATAAGGTGTCTCTGGAAGCATTCAGGGCAAt                                                                                                                                                                                                                                                                                                     >  2:820387/1‑294 (MQ=255)

     tGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTAGTGTAAAAAATATGCTTAAGAGCAACATTTCTATGAGTTACCCTGTTGTTGTAATTGCCTGTATAGAACATAAGGTGTCTTAGGAAGAATTCGGGGCAAt                                                                                                                                                                                                                                                                                                     >  2:183402/1‑294 (MQ=255)

         tAACAAAGTGCGGTCGTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAAAGTGCTCAAATCTTCATACAGAAAGATATGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACACTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAg                                                                                                                                                                                                                                                                                                 <  1:195853/294‑1 (MQ=255)

             aaaGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAg                                                                                                                                                                                                                                                                                             >  1:788500/1‑294 (MQ=255)

                   cGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGT‑TTAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGt                                                                                                                                                                                                                                                                                      <  1:460863/294‑1 (MQ=255)

                     gTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTg                                                                                                                                                                                                                                                                                     >  2:131441/1‑294 (MQ=255)

                        ctgcttGCATTCTTGAGTGAAATACAACCGAAGGATGTATGTAATGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGGAAGCGCAGGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCACCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATGCAGAGCAATTGAGGCAGCGTTGGTGAAg                                                                                                                                                                                                                                                                                  <  2:478624/294‑1 (MQ=255)

                             ggCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACACTGATGTTGTAATTGCATGTATAGAACATCAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGa                                                                                                                                                                                                                                                                             >  2:285269/1‑294 (MQ=255)

                                  tCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGAAACGTTGGTGAAGCACGatcata                                                                                                                                                                                                                                                                        >  2:770333/1‑294 (MQ=255)

                                      gAGTGAAATACAAACGACAGATGTATGTAAGGCCAACGTGCGAAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAatat                                                                                                                                                                                                                                                                    <  2:740682/294‑1 (MQ=255)

                                       aGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAAACTTCATACAGAAAGATTTGAAGTAAGATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATg                                                                                                                                                                                                                                                                   <  2:717087/294‑1 (MQ=255)

                                              tACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGatt                                                                                                                                                                                                                                                            <  2:235481/294‑1 (MQ=255)

                                              tACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGatt                                                                                                                                                                                                                                                            <  1:619589/294‑1 (MQ=255)

                                                cAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGACGCACGATAATAATGTGAGGGattat                                                                                                                                                                                                                                                          >  2:121960/1‑294 (MQ=255)

                                                 aaCCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGattatt                                                                                                                                                                                                                                                         >  1:170308/1‑294 (MQ=255)

                                                     gACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCGAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCGGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCt                                                                                                                                                                                                                                                     <  1:661997/294‑1 (MQ=255)

                                                      aCAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCtg                                                                                                                                                                                                                                                    >  1:718648/1‑294 (MQ=255)

                                                       cAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGACCAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGATTAATAGCCCCATTTCTATGAGTTACCCTGATGTTGTCATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCtgg                                                                                                                                                                                                                                                   >  1:510038/1‑294 (MQ=255)

                                                             tatgtaAGGCAAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTg                                                                                                                                                                                                                                             <  1:873403/294‑1 (MQ=255)

                                                                gtaAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACt                                                                                                                                                                                                                                          <  1:853360/294‑1 (MQ=255)

                                                                      ccAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCAc                                                                                                                                                                                                                                    <  1:368076/294‑1 (MQ=255)

                                                                              cTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGCGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTg                                                                                                                                                                                                                            >  2:153134/1‑294 (MQ=255)

                                                                                 aaaTCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTa                                                                                                                                                                                                                         <  2:85767/294‑1 (MQ=255)

                                                                                  aaTCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTaa                                                                                                                                                                                                                        >  1:221789/1‑294 (MQ=255)

                                                                                     cTTCAGACATAAAGATGTGAAGTAATAAGTTAATCGCGATATGAAGAAGAAGAGCATGGAGCGACAAAATGAATGAAGAACAATCTGCTGATGTTCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTTTAATTGCATGTATACAACATAAGGTGTCTCTGGAAGCATTCAGGTCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCa                                                                                                                                                                                                                     <  2:204578/294‑1 (MQ=255)

                                                                                      ttCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCAt                                                                                                                                                                                                                    <  1:285322/294‑1 (MQ=255)

                                                                                        cATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTc                                                                                                                                                                                                                  >  1:556214/1‑294 (MQ=255)

                                                                                        cATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTc                                                                                                                                                                                                                  >  2:389682/1‑294 (MQ=255)

                                                                                          tACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCaa                                                                                                                                                                                                                <  2:55636/294‑1 (MQ=255)

                                                                                          tACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCaa                                                                                                                                                                                                                >  1:153119/1‑294 (MQ=255)

                                                                                            cAGAAAGATTTGAATGAAGAATTAAAACGCTAGCTGAAGAGCAAAGGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAAc                                                                                                                                                                                                              <  2:239735/294‑1 (MQ=255)

                                                                                                  gATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTa                                                                                                                                                                                                        >  1:97295/1‑294 (MQ=255)

                                                                                                    atgtaaGTAAGAGTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACCATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTagt                                                                                                                                                                                                      <  2:11164/290‑3 (MQ=255)

                                                                                                     tttaaGGAAGATTTTAACCGCTAGATGAAGAGCAACCGCATGGATCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATAATGTACCCTTATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAACACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTagtc                                                                                                                                                                                                     <  1:124059/291‑4 (MQ=255)

                                                                                                      tGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCt                                                                                                                                                                                                    >  1:84728/1‑294 (MQ=255)

                                                                                                        aaGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCtgt                                                                                                                                                                                                  <  1:220472/294‑1 (MQ=255)

                                                                                                         aGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGCTCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCtgtg                                                                                                                                                                                                 >  1:133750/1‑294 (MQ=255)

                                                                                                          gTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCTCTCCGTGGATCTGAATCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTAAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCACTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGa                                                                                                                                                                                                >  1:427285/1‑294 (MQ=255)

                                                                                                             atatTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATACGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACa‑g                                                                                                                                                                                            >  2:296886/1‑294 (MQ=255)

                                                                                                                     aCCGCAAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAaca                                                                                                                                                                                    <  2:256813/294‑1 (MQ=255)

                                                                                                                      ccGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCAGTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAacac                                                                                                                                                                                   <  2:223724/294‑1 (MQ=255)

                                                                                                                                aaGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTctgtc                                                                                                                                                                         <  2:665884/294‑1 (MQ=255)

                                                                                                                                   agCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTctgtcact                                                                                                                                                                      >  2:330964/1‑294 (MQ=255)

                                                                                                                                   agCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTctgtcact                                                                                                                                                                      >  1:743545/1‑294 (MQ=255)

                                                                                                                                   agCAAGCGAATGGAGCGACAAAATGAATAAAGAATAATCTGCTGATGATCCCTCATTGGAGCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATTAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGTAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTctgtcact                                                                                                                                                                      <  2:39967/294‑1 (MQ=255)

                                                                                                                                     cAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTctgtcactgt                                                                                                                                                                    <  1:505452/294‑1 (MQ=255)

                                                                                                                                      aaGCGCATGGGTCGAAAAAATAAATAAAGAACAATCTGCTGTTGATCCCTCCGTGGATCTAATTCGTATAAAAAAGATGCCTAATATCACCATTTCTATGAGTTACCCTGATTTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTctgtcactgtc                                                                                                                                                                   <  2:526435/294‑1 (MQ=255)

                                                                                                                                       aGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTctgtcactgtca                                                                                                                                                                  <  1:299117/294‑1 (MQ=255)

                                                                                                                                            ttgggacGACAAATCAAATAAAGAACAATCTGCTAAGGATCCCTCCGTGGATCTCATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGGAATTGCATGTATAGAACATAAGGGGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGaaa                                                                                                                                                             <  2:591745/288‑1 (MQ=255)

                                                                                                                                            aTGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGaaa                                                                                                                                                             >  1:766451/1‑294 (MQ=255)

                                                                                                                                              ggAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGAAAAAAATATGCTTAATAGCACCATTTATATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGt                                                                                                                                                           <  1:241278/294‑1 (MQ=255)

                                                                                                                                                   gACAAAATGAATAAAGAACAATCTGCTGATGATCTCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCTTGATGTAGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCCATTGAGGCAGCGTTGGTGAAGCACGATAATAATTTGAAGGATTATTCCCTGGTGGTTGACTGATCATCATAACTGTTAATCATACACACTACTTAACCTGT‑ACACGATCCAACACGCAACCTTTACCTGCCAGGAAATTgacaa                                                                                                                                                      >  2:433237/1‑290 (MQ=255)

                                                                                                                                                       aaaTGAATAAAGAACAAGTGGTGGATGATCCCTCCGTGGATTTGAGTCGTGTAAAAAATATTCTTAATAGCACCATTTCTATGAGTTACCGTGATGTTGATATTGCATGTATAGAACATAAGGGGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACt                                                                                                                                                  <  2:887245/294‑1 (MQ=255)

                                                                                                                                                              tAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAATATAAGGTGTCTCTGGAAGCATTCCGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATGATTCCCTGGTGGTTGACTGATCACCATAACCACCAGTCATTCAAACTACTTAACATG‑CACACGAGCCAACACGCAGTCTGACACCGACAGAAAATTGGCaacatgaccacca                                                                                                                                           >  2:509525/1‑283 (MQ=255)

                                                                                                                                                                      agtCTGCTAATGGTCCGTCCTTGGTTCTGATTCGTGTAAAAAATATGCTTAATAGCACCAGTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTg                                                                                                                                   <  2:101054/292‑1 (MQ=255)

                                                                                                                                                                         ctgctgATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTAAAACTACTTAACCTGTAACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAACGTGGTAAAACTGCAACTCAATAACTGCaa                                                                                                                                >  2:470312/1‑294 (MQ=255)

                                                                                                                                                                           ccgaacaatCCGTCCGTGGGTATAATTCAGTTGATAAATATGCTTAATTGCACCATTTCTATGGGTTACCCAGATGTTGGAATTGAATGGATAGAACAGAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAGAGTGGTAAAACTGCAACTCAATTACTGCAATg                                                                                                                              <  2:373917/287‑1 (MQ=255)

                                                                                                                                                                              gatgatCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGccc                                                                                                                           >  1:751375/1‑294 (MQ=255)

                                                                                                                                                                                 gatCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCg                                                                                                                        >  1:307751/1‑294 (MQ=255)

                                                                                                                                                                                   tCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTa                                                                                                                      <  1:685671/294‑1 (MQ=255)

                                                                                                                                                                                   tCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTa                                                                                                                      >  1:830022/1‑294 (MQ=255)

                                                                                                                                                                                    cccTCCGTGGATCGTATTCGTTTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTAGCCTGATGGTGTAATTGCATGTATAGAACATAAGGTGTCGCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTaa                                                                                                                     <  2:622832/294‑1 (MQ=255)

                                                                                                                                                                                     ccTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCCACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCATCGTAAt                                                                                                                    >  2:216857/1‑294 (MQ=255)

                                                                                                                                                                                      cTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAAtt                                                                                                                   <  1:389682/294‑1 (MQ=255)

                                                                                                                                                                                        ccGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑aa                                                                                                                <  2:624960/294‑1 (MQ=255)

                                                                                                                                                                                          gTGGATCTGATTCGTGTAAAAAATATGCTTAAGAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACAGAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGt                                                                                                              <  1:320595/294‑1 (MQ=255)

                                                                                                                                                                                               tCTGATTCGTGTAAATAATATGCGTAATAACACAATTTCTATTGGTTAACAGGAGGTGGTAATTGCATGTATAGAACATAATGGGTCTCAGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAAtt                                                                                                         <  2:680162/294‑1 (MQ=255)

                                                                                                                                                                                                cTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGCAttt                                                                                                        >  1:451920/1‑294 (MQ=255)

                                                                                                                                                                                                       gtgtAAAAAATATGCTTAATAGCACCATTTCTATGTGTTACCCTGATGATGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTGAGGGCAATTGAGGCAGCGTTGGTTAAGCACGATAATACTATGAAGGATTATTCCCTGGTGGCTGACTGATCACCTTAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GCGACAACACGCAGCCTGTCACTGTCAGGAAGGTGGTAAAACTGGCACCCAATTACTGCACTGACCTCGTAATT‑AAGTGAATTTAAAatat                                                                                                 >  2:660709/1‑294 (MQ=255)

                                                                                                                                                                                                       gtgtAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCTATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAatat                                                                                                 <  1:538536/294‑1 (MQ=255)

                                                                                                                                                                                                        tgtAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATc                                                                                                <  1:330964/294‑1 (MQ=255)

                                                                                                                                                                                                        tgtAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATc                                                                                                <  1:861334/294‑1 (MQ=255)

                                                                                                                                                                                                        tgtAAAAAATATGCGTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATc                                                                                                <  1:889877/294‑1 (MQ=255)

                                                                                                                                                                                                                   ttgttAATAGCACCATTTCAATGAGTTACCCTGATTTTATAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATTAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCgg                                                                                     <  1:301016/291‑1 (MQ=255)

                                                                                                                                                                                                                           aGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGCTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGgaaga                                                                             >  2:341752/1‑294 (MQ=255)

                                                                                                                                                                                                                                       aTGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGtt                                                                <  1:236938/294‑1 (MQ=255)

                                                                                                                                                                                                                                        tGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACAAGCAGTCTGTCACTGTCAGGAAAGTGGTAAACCGCCAAC‑CCATTACCGTCAAGCCCCTGTAATTCAGGTGAATTAACAAATACGCCTTTTTCGGAGGGCAG‑ACGCAGGGATGtcc                                                               >  2:517972/1‑292 (MQ=255)

                                                                                                                                                                                                                                        tGAGTTACCCTGAGTTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTc                                                               <  2:356526/294‑1 (MQ=255)

                                                                                                                                                                                                                                             tACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCAttct                                                          <  2:574463/294‑1 (MQ=255)

                                                                                                                                                                                                                                                          tAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTaa                                             >  1:548602/1‑294 (MQ=255)

                                                                                                                                                                                                                                                             ttGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTg                                          <  1:323701/294‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGta                                     >  1:203053/1‑294 (MQ=255)

                                                                                                                                                                                                                                                                  agTATAGAACATAAGGTGTCGTTGGAAGCATTTAGGGCAATTGAGGGAGGGTTTGTGAAGCACGATAATAATATGAAGGATTATTCCCTTGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGta                                     <  2:698244/293‑1 (MQ=255)

                                                                                                                                                                                                                                                                   tgataGAACATAACGTGTCTCTTGGAGCATGCAGGGCAATTGAGGCGGCGTTGGTGAAGCACGATAATAATAGGAAGGATGATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGtat                                    <  2:765805/292‑1 (MQ=255)

                                                                                                                                                                                                                                                                    tataGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATGGATGtata                                   >  2:312616/1‑294 (MQ=255)

                                                                                                                                                                                                                                                                       aGAACATAAGGGGTCTCTGGATGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCAGCATAACAGCTAATAATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGACAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCATCGTAATT‑CAGTGACTTGACAATATCGTACTGTCCAGAGGGAAGAACGC‑GGGATGTTCATTCTTCACCCATTTTAATTGATGTGTATGc                                >  2:180010/1‑294 (MQ=255)

                                                                                                                                                                                                                                                                          aCATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGctct                             <  1:770333/294‑1 (MQ=255)

                                                                                                                                                                                                                                                                            ataagggttgttgggAAGCATTCAGAGCAATTGAGGCAGCGTTTGTGAAGCACGATAATAATATGAAGGAATATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATACAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGctctct                           <  2:709386/281‑1 (MQ=255)

                                                                                                                                                                                                                                                                            aTAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGctctct                           <  1:820387/294‑1 (MQ=255)

                                                                                                                                                                                                                                                                            aTAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCAATTTTAATTGATGTATATGctctct                           >  2:416106/1‑294 (MQ=255)

                                                                                                                                                                                                                                                                            aTAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAAATGCAACTAAATTACTGCAATGCCCTCGTAATT‑AAGTGACTTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGcactct                           >  1:524584/1‑294 (MQ=255)

                                                                                                                                                                                                                                                                                  tgtCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTg                     >  1:646308/1‑294 (MQ=255)

                                                                                                                                                                                                                                                                                    tctcTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAAGATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGAc                   <  2:202652/294‑1 (MQ=255)

                                                                                                                                                                                                                                                                                            aGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGtct           <  1:79697/294‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                ttCAGGGCCATTGAGGCAGCGTTGGTGAAGAACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGa       <  1:101911/294‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                ttCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGa       >  2:106762/1‑294 (MQ=255)

                                                                                                                                                                                                                                                                                                  cAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATCACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACg     <  1:348481/294‑1 (MQ=255)

TTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAACCTGTGACA‑GAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT‑AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGC‑GGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCA  >  NZ_LN832404/576668‑577254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`