Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NZ_LN832404 | 472,148 | A→G | 100% | G84G (GGA→GGG) | glnK → | P‑II family nitrogen regulator |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_LN832404 | 472,148 | 0 | A | G | 96.3% | 363.9 / 2.9 | 108 | G84G (GGA→GGG) | glnK | P‑II family nitrogen regulator |
Reads supporting (aligned to +/- strand): ref base A (3/1); new base G (65/39); total (68/40) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
Only 100 of 119 total aligned reads displayed.
GCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTGGGT > NZ_LN832404/471861‑472433 | gCAGAACCATTACCGAATGCTGAACGGAGGGGATCTATGAAGCGTGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAAtt < 2:364218/294‑1 (MQ=255) aGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTgg < 1:850332/294‑1 (MQ=255) aaCCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGATGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACCGCGTCAATTTCCTGCCAAAA‑GGAAAAATTGATGTGGAGATTGATGATGACCAACTCGAGGAATTGACCGATAGCGGCCGTCAGGCGGGTTACACCGGGACAATTGGCg > 2:464349/1‑294 (MQ=255) aaCCAGTAACGAATTCTGACCGGAGGGGATCGATGAAGCTGGTGACCGTGAGAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTGCCATTGGTATTCAGGGCCTGACCGTCACCGAAGGGAAAGGTGTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCg < 2:3006/294‑1 (MQ=255) aCCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCaa < 1:566245/294‑1 (MQ=255) ccGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGACAATTGGCGACGGCaaa > 1:601863/1‑294 (MQ=255) gAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGCTGACCAACTCGATGAAGTGATCGATATCGTCAGTACGGCGGCTTACACCGGGAAACTTGGCGACGGCAAAAt > 1:462148/1‑294 (MQ=255) cTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTtcg > 1:340368/1‑294 (MQ=255) cGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCt < 1:428681/294‑1 (MQ=255) ggAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCC‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGCTGTGGCGATTGCTGATGACCAACTCGATGACGTGAGAGATATCGTCAGTAAGCAGGCCTGCACCGGGGCAATCGGAGACGGCGAACACTTCGTCGcgg > 2:564817/1‑292 (MQ=255) aGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCTTGCCACAA‑GTAAAAATTGATGTGGCGATTGCTGATGACAAACTCGATGAAGTGATCGATATCGTCAGTACGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATATTGGTCGCTgct > 2:432924/1‑292 (MQ=255) tATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATAGGCAACGGCCAAATCTTCGTCGCTGAATTGCAAcg > 1:181283/1‑294 (MQ=255) aTGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGGTGATGACCAACTCGATGAAGTGATCGATAACGTCAGTAAGGAGGCTTACACCGGTAAACTTGGAGACGGCAAAAGCTTCGTCGCTGAATGGCAAcgc > 2:129406/1‑294 (MQ=255) gAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GGAAAACTTGATGTGGCGAGTGCTGATGACCAACTCGATGAAGTGATCGAGATCGTCAGTAAGGCGGCTTACACCGGGACAATTGGCGACGGAAAAATCTCAGTCGCGGCGTTGCACGGCGt > 2:634727/1‑294 (MQ=255) aTAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTAcc < 1:645525/294‑1 (MQ=255) aTAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATGGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTAcc < 2:224979/294‑1 (MQ=255) tAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAACA‑GTAAAAATTGCTGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCg > 1:675020/1‑294 (MQ=255) aaTCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAGGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACACGTCATTCGTATTCGTACCgg > 1:708671/1‑294 (MQ=255) cATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCcg > 2:310446/1‑294 (MQ=255) cTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAgcg > 1:411793/1‑294 (MQ=255) aGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGAAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCt > 1:150497/1‑294 (MQ=255) ttCGTGAAGCGTTAGCTTCCATGGGGATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAGGCGGCGCTGTAAt < 2:119575/294‑1 (MQ=255) cGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTTGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAAtct > 2:441320/1‑294 (MQ=255) gAAGCGTTATCTTCCATTGGTAGTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTg < 1:849982/294‑1 (MQ=255) ttATCTTCCATTGGTCTTCAGGGCCGGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAAGACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGCTGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGAAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGcacac < 1:201995/294‑1 (MQ=255) cTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTAAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCCACACCTTCGCCGCTGAATTGCAAAGCTTCATCCGTATTCGTACGGGCGAAGCCACCGAAGAGTCGCTTGACTCTGCGGCGCACAG‑Ca > 2:491523/1‑294 (MQ=255) cTTCCATTGGTATTCAGGGCCTGACCGTAACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑Ca < 1:737656/294‑1 (MQ=255) gTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTGAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACg > 1:554570/1‑294 (MQ=255) gTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTACGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCGGAATTGCAACGCGTCATTCGTATTCGTAACGGCGAAGCCGCAGAAGCGGCGATGCAATCTCGGGCACAGCG‑CAAccgaaaac > 2:453382/1‑287 (MQ=255) gTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCTGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CACCAGGAACg > 1:868006/1‑294 (MQ=255) tATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTCAGGAGGCTTACACCGGGAAAATTGGCGACGGCACAATCTTCGTCGCTGCATTGCAACGCGTCATTCGTATTCGTACCGGCGACGCCGACGAAGCGGCGCTGTAATCTCCGGCACACAG‑CAACAGGAACGa > 1:154459/1‑294 (MQ=255) aTTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAACATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGaa > 1:741616/1‑294 (MQ=255) ttCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGaaa > 1:74864/1‑294 (MQ=255) cAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGaaaaa < 1:625771/294‑1 (MQ=255) gACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGTACGAAAAATGAAGATa > 1:39487/1‑294 (MQ=255) aCCGTCACCGAAGTGAAAGATTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAACA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CTACAGGAACGAAAAATGAAGATAg > 1:282292/1‑294 (MQ=255) gtgaCCGAAGGGAAAGGGTGCGGGCGGCAGAAAGGGCATGCCG‑AGCTGGACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAAGTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGcga < 2:610517/291‑1 (MQ=255) gTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATAGCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGcga < 1:821259/294‑1 (MQ=255) gAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGAAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTAGTACCGGACAAGCAGACGAAGCGGCGCTGCAATCTCTGGCACGCCGACAAC‑GGAACGACACAGGAAGATaccagagaaaa > 2:468668/1‑284 (MQ=255) aGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATATTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTCCCGGCGAAGCCGACGAAGCGGCGCTGTCATCGCTGGCACACAG‑CAACAGGCACGAAAAAGGAGGAAAGCGGAGCTaaaa > 2:112514/1‑294 (MQ=255) gTGAAAGGTTTTGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTACAAATTGATGTGGCGTTTGCTTATGACCAACTCGATGAAGTGCTCGATATCGTAAGTACCGCTGCCTACCCCGGGAACATTGGCGACGCCATACTCTTCGTCGCTGTATTTCAACGCGTCATTCTCATTCGTACCGGCGACGCCGTCGAACACGCGCTGTAATCTCCGCGCCCCAG‑CAACAGTGACGCAGAGTGAAGATCGcgtcgacacaac > 2:129076/1‑288 (MQ=255) gTGAAAGGTTTCGGGCTTCAGAAAGGGCATGGCG‑AGCTGTACCGGGGGGCGGAATTCAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATaaaaa < 1:224567/294‑1 (MQ=255) ttaacagtgttgagCGTGAGAAATGGCAGTCCGCAGC‑GTAACGGGGGGCGGAAGACAGCTGCAATATCCTGACAAAA‑GTAAAAATTGATGTGGCGATTTCTCATGACCAACCCGATGAAGTGATCGATATCGTCAATAAGGCGGCTTACACCGGGAAAATTGGCTACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAAGAACGAAAAATGAAGATAGCGACGATAAAAAc < 2:706039/281‑1 (MQ=255) aaaGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGAGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTg > 1:141062/1‑294 (MQ=255) aaGGTTGTGGGCGGAAGAAAGGGCAGTCAG‑AGCGCTACCGGAGGGCGGAATACAGCGTAAATTCAATGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACGAAGTCGATGAAGGGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTgg < 2:634939/294‑1 (MQ=255) cAGAAAGGGCAGGCCA‑AGCTTGAGCGGGGGGCGGAATAAAGCGTAAATTTCCGGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGGTCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTgg < 2:570199/294‑1 (MQ=255) aGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGCACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCCCTggg > 2:362026/1‑293 (MQ=255) aGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACAGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGCACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTAACTGGc > 1:710514/1‑294 (MQ=255) aaaGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGa > 1:404746/1‑294 (MQ=255) aaaGGGCAGGCCG‑AGCTGTGACGGGGGGCAGAATACAGCGTCAATTTCCTGCTAAAA‑GTAAAAATTGATGGGGCGCTTGCTGATTACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTCCCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGa < 1:253449/294‑1 (MQ=255) ggCAGGCCG‑GGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGGGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCt < 1:496222/294‑1 (MQ=255) gCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCgg < 1:143262/294‑1 (MQ=255) ccG‑AGCTGGACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCggg < 2:688688/294‑1 (MQ=255) cG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGa < 1:221912/294‑1 (MQ=255) g‑AGCTGGACCTGGGGGCGGAATAAAGCGTCGAGTTCCTGCCAAAA‑GTAAAAATTGATGATGCGATTGCTGAGGACCAACTCGATGAAGTGGTCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGCCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGAc < 2:181283/294‑1 (MQ=255) gCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTTCAACGAGTCATTCGTATTCGTACCGGCGAAGCCGACGCAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAAGGAAGCTAGCGACGATAAAAACGGGGCTTGCTTCCCTGGCGATGCTGCCGGGACTg > 2:654873/1‑294 (MQ=255) cTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTgg > 1:645801/1‑294 (MQ=255) cGGAATACAGCGTCAATGTCCTGCCATAA‑GTAAAAATTGTTGTGGCGCATGCTGACGACCAACTCGATGAAGTGATCGATACCGTCAGTAAGGCGGCTTACACCGGGCATCTTGGTGACGGCAACATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTCATCTCTGGAACACAG‑CAACAGTCACGCAAAATGAAGATACAGACGATCAAAACTGCGTTGTCTTCCCTGCCGATGCTTCCGGGAGTGTTAATggacgccac > 2:593641/1‑287 (MQ=255) caaCAGGGCCAAGTTCCTGCCAAAA‑GTAAAAATGGAGGTGGCGTATGCTGATGACCAACTCTATGAAGTAATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCg < 2:81610/292‑1 (MQ=255) atacgtcGTCAAATTCCGGCCAAAA‑GTAAAAATTGATGTTGCGATGTCAGATGACCAACTCGATGATGTGAGCGATAGCGTCAGTAAGGCGGCTTACACCGTGAAAATTGGCGCCGGCAAAATCTTCGTCGCGGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCg < 2:611360/288‑1 (MQ=255) aGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGAAGTGGCGACTGCTGCTGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGTTGACTTTCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTTACCCCTGGCACACTG‑CATAAGTAACGAACCATGATGACAGCGACGATAAGAACTTGGCTTGCTTCACTGGCGCGGCTTCCGGTGCCGGTATATGCTGCACCTCCGGTga > 2:632202/1‑293 (MQ=255) cAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCAACCAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCCGAAACTTCGGGTGCCGAt > 2:603031/1‑294 (MQ=255) ccTGCCAAAAGGAAAAAATTGGGGTGG‑GAGTGGTGGTGACCAACTCGAGGAAGTGATCGATATCTGCAGTAAGGCGGCTGACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGcc < 2:744905/294‑1 (MQ=255) gCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCCATGCTTCCGGGACTGGTAATGGCTGCCCCTGCGGCGGCCGATAAAGCCgcc > 2:79744/1‑292 (MQ=255) ccAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTAGCTGAATTGCAACGCGTAATTCGTATTCGTACCGGAGAAGCCGATGAAGCGGCGATGTAATCTCTTGCACACAG‑AAACAGGCACGAAAGATGAAGATAGCGACGATCAAAACCGGGCTTGCTTACCTGGCCATGCACTCGGGACTGGGAATGGCTGTACCTGCGGACGCGGATAAAGcccata > 2:537868/1‑290 (MQ=255) cAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATAGCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTAACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACaa < 1:34191/294‑1 (MQ=255) aaa‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATACAAACTGGGCTTGCTTCACTGGCCATGCATCCGGGACCGGTACAGTCTGCACATGCGGTGGACGATAAAGCCGCCCATg > 2:161860/1‑294 (MQ=255) tAAAAAGTGAGGGGGGGAGTGCTGTAGAGCAACTCGATGAAGGGATCGATATCCTCAGGAAGGGGGCTTACACCGGAAAAATTGGCGACGGCAAAGTCTTCGTCGCTGAATGGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGGACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGtt < 2:789413/294‑1 (MQ=255) aaaTTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGACGATAAAGCCGACAATGCGTTTat > 1:532822/1‑294 (MQ=255) ttGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAACGCCGACACTGCGGTTatggt > 2:318179/1‑292 (MQ=255) tGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAACACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGCGGCCGATAAAGCCGTCTAAGCGTTTgtgatg > 2:537140/1‑294 (MQ=255) tgtgGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGAtt < 1:310446/294‑1 (MQ=255) ggCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTCATGGCTGCACCTGCGGTGGCCGATCAGGCCGACAATGCGTTTCTGATGATTAGt > 2:771251/1‑294 (MQ=255) ggCGATTGCGGATGCCAAACGCTATGAAGTGATCGATAGCGTCAGTAAGGCGGCTTACCCCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGt < 2:202644/294‑1 (MQ=255) aTTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATATAGTCGACAATGCGTTTATGATGCTTTGTACTg > 1:330016/1‑294 (MQ=255) tggCTGATGCGCAACCGGATGAGGTGATCGATATCGGCGGCAAGGGGGGTAACACCGGGAAAATTGGGGACGGCAAAATCTTCGCGGCTGAATTGAAACTCGTCATTCGTATTGGTACCGGCGAGGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTgc < 2:484458/292‑1 (MQ=255) gCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCAATGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATACAGCCGACCATGCGTTTATGATGATTTGTACTgcgc > 1:875222/1‑294 (MQ=255) gatgaCCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTgg > 1:741735/1‑294 (MQ=255) cAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACCATGCGTTTATGATGGTTTGTACTGCGCTGGTGCTgc > 2:497914/1‑293 (MQ=255) gagttgggtgagggATCGGTATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGAAAAGTCTTCGTCGTTGAATTGAAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGGGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGtt < 2:472415/281‑1 (MQ=255) gATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGa < 1:368420/294‑1 (MQ=255) gATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGa > 1:841895/1‑294 (MQ=255) aGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTAtt > 1:786372/1‑294 (MQ=255) gTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGCCGAAGCGGCGCTGTCATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACCGGTAATGGCTGCGCCTGCCGTGGCCGATCAATCCCACAAAGCGTTTATGCTTACTTGTTCTGCGCTGGTGCTGTTTATGAcgactc > 2:164772/1‑289 (MQ=255) gTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCGTCGGTGGCCGATAAAGCCGACAATGCGTTTGTGATGGTTTGGACTGCGCTGGTGCTGTTTAGGAatatcc > 2:480748/1‑292 (MQ=255) gATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGACGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTAGCTGCGATGAAGATGCTTACGGGACTGATAATGGCTGCACCAGCGGTGGCCGCTAAAGACGACAATGCGTTCATGAGGATTTGAACTACAGTGGCAGTGTTTATGACTAtaaag > 2:66339/1‑290 (MQ=255) gATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGCATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACGGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACGGCGCTGGTGCAGTTCATCCCTATTCCGGGGa > 2:843674/1‑294 (MQ=255) gATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTCATCTCTGGCACACAG‑CACCAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTAGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGCTACAGCCGACAATGAGCTTATGATGCATGGTACTGCGCGCGTGCTGGTTATGACTATTACGGGGa > 2:404329/1‑294 (MQ=255) gATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGAAAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGa > 1:339267/1‑294 (MQ=255) gATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTAAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGCACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTCATGGCTGCACCTGCCGTGTCCGATAAAGCCGACCATGCGTTTATGATGCTTCGTACTGCGCTGGTGCGGTTTAAGCCGCTTCCAGGGa > 2:546191/1‑294 (MQ=255) tatCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACCATGCGTTCATGATGATTTGTACGTCGCTGGTGCTGTTTATGACGATTCCGGGGAtt > 2:170205/1‑294 (MQ=255) cGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGCTTGcc > 1:675046/1‑294 (MQ=255) cAGTAAGGCGGCTTACACCGGGAAAATTGGAGACGGCAAAATCGTCGTCGTGGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAAGGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTg < 1:504603/294‑1 (MQ=255) aatgaaGGCGGCTTACACAGGGAAAATTGGCGACGGCAAAAGTCTCGGCGCGGAATTGCACCGCGTCATTCGTAATCGATCCGGCGAAGCCGACGAAGCGGCTGTGTAATCTCTGGCACACAG‑CAACATGAACGAAAAATGAAGATAGCGACGATAAGACCTGGGCTTGCGTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTg < 2:710514/290‑1 (MQ=255) aGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGTTGGCTGATAAAGCCGACAATGCGTTTATGCTGATTTGTACTGCGCTGGTTCTGTTTATGCATCTTCCGGGTATTGctgtgg > 2:101103/1‑289 (MQ=255) aGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGGTTATGAGGATTTCTACTGCGCTGGTGCTGGTTAAGACTATTCCGGGGATTGGCCTGt > 2:231983/1‑294 (MQ=255) aaGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGtttt > 1:670169/1‑294 (MQ=255) ggcTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGt > 2:706109/1‑294 (MQ=255) cTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTTGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTgg < 1:441320/294‑1 (MQ=255) tACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTGGGt < 1:550820/294‑1 (MQ=255) | GCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCG‑AGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAA‑GTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAG‑CAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTGGGT > NZ_LN832404/471861‑472433 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |