| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_LN832404 | 4,023,921 | G→A | 64.7% | intergenic (+220/+161) | EK12AG100_RS19960 → / ← fadA | NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_LN832404 | 4,023,921 | 0 | G | A | 64.7% | 2.1 / 13.7 | 17 | intergenic (+220/+161) | EK12AG100_RS19960/fadA | NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (8/3); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01 | |||||||||||
CCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACC > NZ_LN832404/4023629‑4024210 | ccGCGATCTGTTTTGCAGTGAGCGGAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGt < 1:661284/294‑1 (MQ=255) gATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt > 1:464085/1‑294 (MQ=255) aTCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCCATTGGTGCCTGATGCCTGATGGGCCGCTGGGCCGTCTTATCCGGCCTa > 2:193735/1‑294 (MQ=255) tCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAc > 1:6682/1‑294 (MQ=255) aGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACTAATAGTCCGAACCGTAGGCCGGATAAGGCGGTTACGCCGCATCCGGCAACTGGTGCCGGGTGCCTGATGCGACGCTGGGCCGTCTTATCAGGACTACattggttccg > 2:255465/1‑285 (MQ=25) gTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACattggtgccgg > 1:228626/1‑284 (MQ=255) gAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGCCGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGGACCGTAGGCCGGATAAGGCGTTTACGCCCCACCCGGCCATTGGTGCATGATGCCTGTCGCCACGCACGCGCTCCTTATCCGGCCTACattggtgccggcc > 2:39271/1‑282 (MQ=25) gCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGttc < 1:383580/294‑2 (MQ=18) ccTGTGGGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGttca < 1:549634/294‑3 (MQ=18) gTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCT‑CCATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgc > 1:399649/1‑294 (MQ=18) aTGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCATCTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCg < 1:640829/294‑1 (MQ=17) gTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGCTAAGGCGTTCACGCCGCATCCGGCaa > 1:115414/1‑294 (MQ=17) ttATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaa > 1:91164/1‑291 (MQ=17) ttATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaa > 1:16710/1‑291 (MQ=17) gaTATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaaacccgctc > 1:581241/1‑283 (MQ=14) aCCATGCCCGGGGCGCAGACGTTGCGTGTTATCGTGGCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGGCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGtt < 2:570800/294‑1 (MQ=25) ctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGAtt > 1:152755/11‑294 (MQ=255) acctcgCCGGATGCTCCGCTGTAGGGTCTTTCTGGGCCTACAAATATT‑CC‑GAAACTTGGTCCGGAGAAGGCATTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCagcagc < 2:516760/289‑1 (MQ=37) tGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATAAACATCGTCGCCCGACCCAACTGAACGACTTTGCCTTCAACACGATTCACCAGCgtgggcctgcc > 2:169521/1‑287 (MQ=25) gcggcgCTGGCGTGTCTGATCGTGCCTACAAATAGT‑CC‑GAACGTTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGGGCATGATGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATa < 2:175119/294‑1 (MQ=255) gACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGCCCAAACTGAACGTTCTTGCGTTCCATCAGACTCaacagccgggttctcatccc > 2:71307/1‑280 (MQ=255) ggcgcgTGTTATCGGGCCGAAAAAGAGT‑CC‑GAACCGGAGGCCGGATAAGGCGTTTACGCCGCAGCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCAcc < 2:115221/292‑1 (MQ=255) tATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCCCCGGACCAAcc > 1:200642/1‑294 (MQ=255) | CCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACC > NZ_LN832404/4023629‑4024210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |