Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 4,220,111 T→C 100% intergenic (‑10/‑190) iclR ← / → metH glyoxylate bypass operon transcriptional repressor IclR/methionine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324044,220,1110TC97.8% 324.9 / ‑2.1 92intergenic (‑10/‑190)iclR/metHglyoxylate bypass operon transcriptional repressor IclR/methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (38/52);  total (39/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACG  >  NZ_LN832404/4219827‑4220400
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                 
ccGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGt                                                                                                                                                                                                                                                                                          <  1:439816/294‑1 (MQ=255)
   atcatataggctgatgCGCCGAGTGTCCAAAGATCCGGTTCGCCAACCGGGCGTACGAAACGCTGCTGT‑GGCATCCTGGTTAGCCGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGG‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATc                                                                                                                                                                                                                                                                                       <  2:541734/280‑1 (MQ=255)
   aCCGTAAGGGGATGGGCGCCGATTGCCCAAAGTCCCGGTTCGCCAACCTGACGAACGAAACCCTGCTGT‑TGCATCGTGGGTAGCAGGCGGTGGGTCGTGGAATGGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATc                                                                                                                                                                                                                                                                                       <  2:306394/294‑1 (MQ=255)
    ccATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTGTTTCTGTATCg                                                                                                                                                                                                                                                                                      >  1:727275/1‑294 (MQ=255)
      aTAAAGGCATGTGCGCCGATGGCCCAATGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTg                                                                                                                                                                                                                                                                                    <  1:522259/294‑1 (MQ=255)
        aaaGGCAGGTGCGCCGATTGCCCAATGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGa                                                                                                                                                                                                                                                                                  <  1:191498/294‑1 (MQ=255)
           ggCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCGATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGGTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAt                                                                                                                                                                                                                                                                               >  1:136658/1‑294 (MQ=255)
                       gATTGCCCAATGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATcattttcatttt                                                                                                                                                                                                                                                                   >  1:720380/1‑294 (MQ=255)
                       gATTGACCAATGTCCCAGTGCGCCAACCGGACGAACGAAACCCGGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATcattttcatttt                                                                                                                                                                                                                                                                   <  1:820674/294‑1 (MQ=255)
                           gCCCAATGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTGGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTCTCTGTATCGTGGAAATCCTTTTCCTTTTTAtt                                                                                                                                                                                                                                                               >  1:842373/1‑294 (MQ=255)
                           gCCCAAGGGCCCATGTCGCCAACCTGACGCACGAAACCTGGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtt                                                                                                                                                                                                                                                               <  1:473521/294‑1 (MQ=255)
                                atatcccatgtgcccAACCTGATGCACTAAACCCTG‑TGTGTGCATCGTGGTGCGCAGGCGATGGTTCTTGGAATTGGGTAAACCGGCTTGTTGCTCCAGTTCTGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACGCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAg                                                                                                                                                                                                                                                          <  2:797106/281‑1 (MQ=255)
                                aTGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTGCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGCCGGGTTTTCCGCCGCGTTTCGCGGGACAGGTCGGGCACACGAGCGTCTACTTTTTCTCTATCCGGGAAACCGCTTTCAGTTTTAttttctg                                                                                                                                                                                                                                                          >  2:239206/1‑289 (MQ=255)
                                  gTCCCAGTTCGCCCACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCt                                                                                                                                                                                                                                                        <  1:219677/294‑1 (MQ=255)
                                      cAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTTGCTTATg                                                                                                                                                                                                                                                    >  1:346000/1‑294 (MQ=255)
                                      cAGGTCGCCAAGCGGACGCACGAAACCCTGCTGT‑TGCATCGTGGTGAGCAGGCGGTGGGTCGTGTAATTGTGTAACCCGGCTTGTGGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCGGTAATGTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATg                                                                                                                                                                                                                                                    <  2:162284/294‑1 (MQ=255)
                                       aGTTCGCCAACCTGACTCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGTTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACGGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGc                                                                                                                                                                                                                                                   <  1:388683/294‑1 (MQ=255)
                                            gCCAACCTGACGCACGAAACCCTTCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAg                                                                                                                                                                                                                                              <  1:796201/294‑1 (MQ=255)
                                             ccAACCTGACGCACGAAACCCGGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt                                                                                                                                                                                                                                             <  1:687414/294‑1 (MQ=255)
                                                aCCTGACGCACGACACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTac                                                                                                                                                                                                                                          <  1:142828/294‑1 (MQ=255)
                                                      cGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaact                                                                                                                                                                                                                                    <  1:106931/294‑1 (MQ=255)
                                                       gCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactg                                                                                                                                                                                                                                   <  1:103245/294‑1 (MQ=255)
                                                            aaaCCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGCCCATTACCGTCTCCTTTTTCTGTATTGTGGGAATCATTTTTCTTTTTATTGTTAGCTAATGGAATAGTTACTGAACTGATCCg                                                                                                                                                                                                                              >  2:717548/1‑294 (MQ=255)
                                                             agcccTGAAGT‑TGCATCATGGTGAGCAGGCGGTGGATCGGGGAATTGGGTAACCCGGCTTGTTGCGCGAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGACACGCGTTAAAGACTGAACCTGTCCAGTCTGTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAATTCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                                                                                                                                                                                                                             <  1:223321/292‑1 (MQ=255)
                                                               accTGCTGT‑TGCATCGTGGTGAGCAGGCTGTGTGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTGCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATg                                                                                                                                                                                                                           <  1:728016/293‑1 (MQ=255)
                                                                    ctgT‑TGCATCGTGGTTAGCAGGGGGGGGGTAGTGGAATGGGGTAACCCGGCGTGTTGCGCCAGGTCCGTGAGG‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTGAAAGACGGACCCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTa                                                                                                                                                                                                                      <  2:882892/294‑1 (MQ=255)
                                                                    ctgT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTa                                                                                                                                                                                                                      >  1:860718/1‑294 (MQ=255)
                                                                       t‑tGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTTGCTAATGCAATTGTTTCTGAACTGATTCGTTGTGTTAATg                                                                                                                                                                                                                   >  2:554416/1‑294 (MQ=255)
                                                                          gCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACCGTCTCCTTTTTCTGTATCGTGGACATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATACGATGAGTTAATGtt                                                                                                                                                                                                                 >  1:356334/1‑294 (MQ=255)
                                                                          gCAGCGTAGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCAGTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTGTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGtt                                                                                                                                                                                                                 <  1:191776/294‑1 (MQ=255)
                                                                           cATCGTGGTTGGTGGCGGGTGGGGCGTGGAATTGGGTACCCCTGCTTGTGGCGCCAGTGCCGGGAGT‑GCCATACTGCCATTGGATTCGGCAATCCACGCCAGTAATTTCAGGCCACGCGTGAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTg                                                                                                                                                                                                                <  2:595668/294‑1 (MQ=255)
                                                                                  gTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGGTTCGCGGGAATGGGTGCGACCATGCCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTCTTGTTAGCTCATGCAATAGTTACTGAACTGATACGATGAGTTAATGTTCGACAAAt                                                                                                                                                                                                         >  1:413308/1‑294 (MQ=255)
                                                                                       cggggggTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAGCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCAt                                                                                                                                                                                                    <  1:280728/289‑1 (MQ=255)
                                                                                            ggTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCATGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGCAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGc                                                                                                                                                                                               >  1:140183/1‑294 (MQ=255)
                                                                                            ggTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGCAATGGGTGAGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCAGTTTCATTTTTTTTTTTAGCTAATGCAATAGTTACTGAACTGATCCTATGGGTAAATGTTTATCAAATCTCCTGttta                                                                                                                                                                                               >  2:133832/1‑292 (MQ=255)
                                                                                            ggTGGGTCGAGGAATTGGGTAACCCGGCTTGTTGAGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCTGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGACTGGGTGCGACCATGAAAGTCCCCTTCTTCTGTACCGTGGAAATCCTTTGCATTTTTTTCTTTCGCTCATGCCATACTTCATGACCTAACCCGATGATTTCATGTTGAATAACTCTCCTTTTgg                                                                                                                                                                                               >  2:334776/1‑293 (MQ=255)
                                                                                                ggTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCCCCTTTTTCTGTATCGTGGACATCATTTTCATTTTTATTGTTAGCTAATGCAATAACTACTGAACTGATCCGATGAGTTACTGTTGAACAAATCTCatgatggctgg                                                                                                                                                                                           >  1:870766/1‑289 (MQ=255)
                                                                                                    gTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGCCAGTCTCCTTTTTCTGTATCGTGGAAATCCTTTTCATTTTTCTTGTTAGCTGATGCACTAGTTACGGAACTGTGTAGATGAGTTAATGTTGCACAACTCCCAGGTTGCgtgtgggc                                                                                                                                                                                       >  2:562095/1‑289 (MQ=255)
                                                                                                      ggAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTCTTGCCATAGTTACTGAACTGATCCGATGGGTTAATTTTGAACACATCTCATGTGGCGTGGTTGTCg                                                                                                                                                                                     >  2:45765/1‑294 (MQ=255)
                                                                                                          ttGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCttt                                                                                                                                                                                 <  1:256764/294‑1 (MQ=255)
                                                                                                          ttGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCttt                                                                                                                                                                                 <  1:787961/294‑1 (MQ=255)
                                                                                                            gggTTACCGGGCTTGTTGCGCCAGTTCCGTGAGT‑GACACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGCACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGGCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTa                                                                                                                                                                               <  1:169851/294‑1 (MQ=255)
                                                                                                                  cccGGCTGTTAGCGCCAGTTCCGTGAGTGGCCACAAT‑CCATTGGATTCGGAAATCCACTCCAGTGAGTTGAGGCCAAGCGTGAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGTGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAg                                                                                                                                                                         <  2:60407/294‑1 (MQ=255)
                                                                                                                    cGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGAt                                                                                                                                                                       <  1:292286/294‑1 (MQ=255)
                                                                                                                     ggCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATg                                                                                                                                                                      <  1:554416/294‑1 (MQ=255)
                                                                                                                     ggCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATg                                                                                                                                                                      >  1:782052/1‑294 (MQ=255)
                                                                                                                             gcggcggtgctgTGAGT‑GCCACGCTGCCATTGGGTTCGGCAGTCCACGCCTGTGATTTCAAGTCGCGCGTTAAAGACTGGTCCTGTCCAGGCGCTGGTGCTGT‑GGCAATGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAGGCAATAGTTACTGAACTGATCCGATGAGTTGATGTTGAACTAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATg                                                                                                                                                              <  2:280140/283‑1 (MQ=255)
                                                                                                                              cgcCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCCTTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTTCGTGGTGGTCGCTTTTACCACAGATGCGTTGATGc                                                                                                                                                             >  1:611471/1‑294 (MQ=255)
                                                                                                                               gcCAGTTCCGTGAGT‑GCCACACTGCCATTTGATTCAGCAATCCCCTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCGTGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTGTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGTTCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGcc                                                                                                                                                            <  1:426371/294‑1 (MQ=255)
                                                                                                                                   gTTGCGTGAGG‑GCCAGCATGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑TGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTa                                                                                                                                                        <  2:285563/294‑1 (MQ=255)
                                                                                                                                     ttcGTGGGG‑GCCACCCTGGCATTGGGTTCGGCAATCCACGCCAGTAAGTTCAGGGCACGCGGTAAAGACTGAACCTGTCCAGGCGCTGGGGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATg                                                                                                                                                      <  2:116387/292‑1 (MQ=255)
                                                                                                                                                 cacaCTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAAt                                                                                                                                           <  1:378100/294‑1 (MQ=255)
                                                                                                                                                    acTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAAtttt                                                                                                                                        <  1:770882/294‑1 (MQ=255)
                                                                                                                                                     cTGCCAGTGGATTCGGCAATCCACTCCAGTAGTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAAttttt                                                                                                                                       <  2:54085/294‑1 (MQ=255)
                                                                                                                                                       gCCATTGGATTCGGCAATCCACTCCAGTAAGTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTttat                                                                                                                                     <  1:331321/294‑1 (MQ=255)
                                                                                                                                                        ccATTGGATGTGGCATACCACGCCAGTAATTTCAGGCCACGCTGTAAAGGCTTAACCTGTCCAGACGCTTGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTTCTACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAACTCTCATGTTGCGTGGTTGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTttatt                                                                                                                                    <  2:585188/294‑1 (MQ=255)
                                                                                                                                                              gagtCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCt                                                                                                                              <  1:351483/291‑1 (MQ=255)
                                                                                                                                                               gttCGGCAATCCACTCCATGAATTTCAGGCCACGCGTTAAAGACTTGACCTGTCCAGTCGCTGGTGCGGT‑TGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTg                                                                                                                             <  2:578976/293‑1 (MQ=255)
                                                                                                                                                                     cAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAACTCTACTGTTGCGTGGTGGTCGCTTTTACCACCGATGCGTTTATGCCAGTATGGTTTGTTGCATTTTTATTAAATCTGGGTTGa                                                                                                                       >  1:580107/1‑294 (MQ=255)
                                                                                                                                                                            cTCCAGTAATTTCAGGCCACGCGTTACAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCCCGGGCCTGGGTGCGACCATGACAGTCCCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATTCATTAGTTACTGAACTGCTCCGATGAGTTCATGTTGACACAATCCCATGTTTCGTGGTGGTCGCTTTTACCCCAGTTGCTGTTGTGCCCGTATTGTTTGTTTGATTTTTATTAAATACGGGTTGAGCGTGTc                                                                                                                >  2:371012/1‑294 (MQ=255)
                                                                                                                                                                            cTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACCGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTc                                                                                                                <  1:601370/294‑1 (MQ=255)
                                                                                                                                                                                aGTAATTTCAGTCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGa                                                                                                            <  1:743527/294‑1 (MQ=255)
                                                                                                                                                                                    aTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGAGTGTCGGGAGCaa                                                                                                        >  2:3837/1‑294 (MQ=255)
                                                                                                                                                                                     tttCATGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCCAGTTTTGCGGGCATTGGTGCGACCATGACAGTCCTCTTTTTCTGTATCGTGTAAATCATTTTCATTTTTATTATTACCTAATGCAATAGTTTCTGAACTGATCTTATGTGTTAATGTTGAACAAATTTCTTGTTTCGTTGTGTTCACTTTTACCACAGATTCGTTTATGCCTGTATGGTCTGTTTAATTTTTATATATTCTGGATTGAGCGTTTCgagttgtag                                                                                                       >  2:396493/1‑286 (MQ=255)
                                                                                                                                                                                                  gTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCaaa                                                                                          <  1:109407/294‑1 (MQ=255)
                                                                                                                                                                                                    tAAAGATGGAACCTGTTCGTTAGCT‑GTGGGGTGGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCTACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGTTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTGTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGt                                                                                        <  2:685736/294‑1 (MQ=255)
                                                                                                                                                                                                    tAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTCTTATATCTGGGTTGAGCGTGTCGGGCGCAAGTGTGAGCAGCAAAGt                                                                                        >  1:862987/1‑294 (MQ=255)
                                                                                                                                                                                                    tAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGt                                                                                        <  2:647146/294‑1 (MQ=255)
                                                                                                                                                                                                        ggttGAACCTGTCCAGTCGCTGGTGCGGT‑GGTAAGGGCGGGTTTTCTGCCGGGTTTCGCGGGAATGGGGGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaa                                                                                    <  2:529507/291‑1 (MQ=255)
                                                                                                                                                                                                        gACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTTAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaa                                                                                    >  2:766075/1‑294 (MQ=255)
                                                                                                                                                                                                         aCTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGGGTGAGCAGCAAAGTGGaac                                                                                   >  1:362576/1‑294 (MQ=255)
                                                                                                                                                                                                          cTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGACATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGCTGAGTTTCAGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTATAGCGTGTCGGGCGCAAGTGTGTGCAGCACAGTGGcaca                                                                                  >  2:684531/1‑294 (MQ=255)
                                                                                                                                                                                                            gAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaacaac                                                                                <  1:45765/294‑1 (MQ=255)
                                                                                                                                                                                                             atctttgccAGTGGGTGGTGGGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGTGAATGGGTGCTACCATGACAGTTTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGGTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACt                                                                               <  2:626290/287‑1 (MQ=255)
                                                                                                                                                                                                               ccTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgc                                                                             <  1:717548/294‑1 (MQ=255)
                                                                                                                                                                                                                cTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGTAAGTGTGGGCGGCAAAGTGGAACAACtgcg                                                                            >  2:28972/1‑294 (MQ=255)
                                                                                                                                                                                                                  gTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGCAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTAGTGAACTGATACGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGTTGCGTTTATGCCAGTATGGTTTGTTGATTTGTTAGTAAATCTGGGTTGAGCGTGTCCGGAGCAAGTGTGAGCAGCAGAACGGAACAACtgcggg                                                                          >  2:246094/1‑292 (MQ=255)
                                                                                                                                                                                                                  gTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGACTTTTTATTAACTCTGGGTTGAGCGTGTCGGGCGCAAGTTTGTGCAGCTAAGTGCAAAAACtgactg                                                                          >  2:768987/1‑290 (MQ=255)
                                                                                                                                                                                                                   gcctgTCGCGGGTGCGGT‑GGCAACGGCGGGTTTTCTTCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgtgc                                                                         <  2:136658/290‑1 (MQ=255)
                                                                                                                                                                                                                     cAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATACGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGCTGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGTGTGTCTGGAGCAAGTGTGAGCAGCAAAGTGGAAAAACTGCGTgcgc                                                                       >  2:763451/1‑294 (MQ=255)
                                                                                                                                                                                                                       gtcgggggTGCGGT‑GGCGACGGCGGGTTGTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTAGCCTTTTTCAGTATCGTGGAAATCATTTTCATTTTTATTGGTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCGTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAg                                                                     <  1:141458/288‑1 (MQ=255)
                                                                                                                                                                                                                       gTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGCACAACTGCGTGCGCAg                                                                     >  1:31325/1‑294 (MQ=255)
                                                                                                                                                                                                                           cTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaa                                                                 <  1:177358/294‑1 (MQ=255)
                                                                                                                                                                                                                           cTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaa                                                                 <  1:77737/294‑1 (MQ=255)
                                                                                                                                                                                                                             ggTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGGTGCGTTTATGCCCGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGTGCGTTTCGGGAGGAAGTGTGCGCCGTAAAGTGGAACAAGTGCGTGGTCAGTGAAAt                                                               >  2:176577/1‑294 (MQ=255)
                                                                                                                                                                                                                             ggTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAAt                                                               <  1:766075/294‑1 (MQ=255)
                                                                                                                                                                                                                                       gCAACGGCGGGTTTTCTGCCGCGTTCCGCGGGCATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCCATAGTTACTGAACTGATCCGATGAGATAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGTTTTTACCACAGATGTGTTTATGCCACTTTGTGTTGTTGAATTTTTATTAAATCTGCGTTGAGGGTGTCGGGATCACGTGTGCGGCTCAACGTGGACACACGGGGTGCGCAGtatattggcctgact                                                      >  2:652582/1‑280 (MQ=255)
                                                                                                                                                                                                                                         aaCGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACCGTCTCCTTTTTCTTTTTCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAAATGATCCGATGAGTTCATGTTTAACAAATCTCATGTTGTCTGGTGGTCGGTTTTTCCACAGATGCGTTTATGCCAGTATGGTTTGTTGAGTTTTTATTAAATCTGTGGTGAGAGTGTCGGGAGAAAGTGTTAGCAGAAAACAGGAACACCTGCGTTCGAAGTTAAAATAGCGTATTCt                                                    >  2:822213/1‑294 (MQ=255)
                                                                                                                                                                                                                                          aCGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg                                                   >  2:343904/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                         tGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATcc                    >  2:114677/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                           ggTGGGACCATGTCTGTGTCCTTTTTCTGGTTCGTGGAAATCATTTTCATTTTGATTGTTAGGTAATGCAATAGTTACTGAACGGATCCGATGAGTTAATGTTGAACAAAGCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCag                  <  2:842373/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ggTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCag                  >  1:501305/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                             tGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCagag                >  1:861581/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                   cATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGCACAAATGCGGGCGCAGTTAACTGAACGGATTCTGGTGCTGGACGGCGGGAGGGGCACCAGACTCCAGAGTTATCg          >  2:130884/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                           tctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGTCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACg  >  1:62484/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                 
CCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCAACCTGACGCACGAAACCCTGCTGT‑TGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGT‑GCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGT‑GGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACG  >  NZ_LN832404/4219827‑4220400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑