BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	666,777	G→T	P481T (CCT→ACT)	mrdA ←	penicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	666,777	0	G	T	99.2%	933.1 / NA	249	P481T (CCT→ACT)	mrdA	penicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (123/123); total (125/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCA  >  NC_000913/666533‑667022

tgCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCtt                                                                                                                                                                                                                                                 <  2:1801221/251‑1 (MQ=255)

      ttATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGAt                                                                                                                                                                                                                                           <  2:1494484/251‑1 (MQ=255)

         taGGTTTCGTTGGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAAGTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGGGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATAcc                                                                                                                                                                                                                                        <  2:968520/251‑1 (MQ=255)

               tcgttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCAtt                                                                                                                                                                                                                                  <  1:1623706/251‑1 (MQ=255)

               tcgttcgCGTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCAtt                                                                                                                                                                                                                                  <  1:2451479/251‑1 (MQ=255)

                  ttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAAt                                                                                                                                                                                                                               >  2:1904546/1‑251 (MQ=255)

                   tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATGTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCTGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATc                                                                                                                                                                                                                              <  1:577/251‑1 (MQ=255)

                      cttcaaGACCGAAGACCTAAGCGGTACCGGATTTCGCCGCAAGTTTGTACGGTGCGCTAGCAAAGTATTGATGCGCGGAACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAgg                                                                                                                                                                                                                           <  1:914304/246‑1 (MQ=255)

                        ttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGat                                                                                                                                                                                                                         <  2:1669024/251‑1 (MQ=255)

                        ttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGat                                                                                                                                                                                                                         <  1:1904382/251‑1 (MQ=255)

                        ttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGat                                                                                                                                                                                                                         <  2:1105/251‑1 (MQ=255)

                           aGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGatcat                                                                                                                                                                                                                      >  2:2532368/1‑251 (MQ=255)

                             aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGAtcatca                                                                                                                                                                                                                    <  2:1996927/251‑1 (MQ=255)

                              ccGAAGACCTGCGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGACACACCGTACATCCCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATAACGCCGACGGGCGGTTCATGCGGCTGTACTCATGGCCCCTGTTTGCCGTCTTCTGCGGTGCGCATCAGCAACTGAGTCACCTTCAAGATaactccatacatgtggaccatcag                                                                                                                                                                                                                   >  1:250276/1‑228 (MQ=255)

                              ccGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTTTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAAACTTCACGATACCGt                                                                                                                                                                                                                                      >  2:2069507/1‑232 (MQ=255)

                              ccGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAg                                                                                                                                                                                                                   >  2:140935/1‑251 (MQ=255)

                              ccGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCATCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAg                                                                                                                                                                                                                   >  2:1122202/1‑251 (MQ=255)

                                     ccTGAGCTGTACCGGATTTCGCCGCAATATTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGAACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAAGGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCAtt                                                                                                                                                                                                                                  <  2:290046/229‑1 (MQ=255)

                                           cGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTGATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATc                                                                                                                                                                                                      <  1:64499/251‑1 (MQ=255)

                                             gTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCAt                                                                                                                                                                                                                                   <  1:1132690/220‑1 (MQ=255)

                                              tACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTgg                                                                                                                                                                                                   >  1:1668891/1‑251 (MQ=255)

                                              tACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTgg                                                                                                                                                                                                   <  2:2288188/251‑1 (MQ=255)

                                              ncactgATTTCGCCGAAATTGTGTACGGGTGGCTGGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTgg                                                                                                                                                                                                   <  1:1430539/246‑1 (MQ=255)

                                                   gATTTCGCCGCGCTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCCTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTgg                                                                                                                                                                                              <  1:443517/251‑1 (MQ=255)

                                                      ttCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGAt                                                                                                                                                                                                 >  2:1592697/1‑245 (MQ=255)

                                                        cgccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCg                                                                                                                                                                                         <  2:2205278/251‑1 (MQ=255)

                                                             catcttttTACGTTGCGCTAGCAAAGTTTTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTATCCGTAATGAATATCGCCGACTGTCTGTTCATGCTGCTGTCCCCGTGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTc                                                                                                                                                                                    <  2:1609913/247‑1 (MQ=255)

                                                             cAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGCAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCTCCTGTTTGCCGTCTTCGGCGGTGCTCATCGGCAACTGAGTCACCTTCACGATTCCGTCATTAATCAGGCTCACCAGAGTCTTCCtatgttacttatacccctttgaa                                                                                                                                                                                    >  1:290025/1‑229 (MQ=255)

                                                               aTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGatca                                                                                                                                                                                                                       <  2:854996/214‑1 (MQ=255)

                                                                     tACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAAcc                                                                                                                                                                                                                                                   >  1:2454553/1‑180 (MQ=255)

                                                                     tACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTAc                                                                                                                                                                                                           >  2:1132773/1‑220 (MQ=255)

                                                                     tACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTAc                                                                                                                                                                                                           >  1:1804514/1‑220 (MQ=255)

                                                                     tACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAAcc                                                                                                                                                                            >  1:40023/1‑251 (MQ=255)

                                                                         gTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGAt                                                                                                                                                                                                                                           <  2:231807/184‑1 (MQ=255)

                                                                           gcgcTAGCAAAGTATTGAAGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGAcc                                                                                                                                                                      <  1:652706/251‑1 (MQ=255)

                                                                            cgcgAGCAAAGTATTTATGGGCCGTACCGTTAGGGCCGTTAGCAACACCGTACTTACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGGCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCg                                                                                                                                                                     <  1:1103120/251‑1 (MQ=255)

                                                                                       tATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCCCCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAg                                                                                                                                                                                                                   >  1:931691/1‑194 (MQ=255)

                                                                                       tATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACg                                                                                                                                                                                                                                             >  2:2129329/1‑168 (MQ=255)

                                                                                       tATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATCCCGTCATTAATCAGGCTCATCAGTGCCTTACTCATCTGGATTg                                                                                                                                                                                               >  1:854935/1‑214 (MQ=255)

                                                                                       tATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCgg                                                                                                                                                          >  2:1418227/1‑251 (MQ=255)

                                                                                         ttttTGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCa                                                                                                                                                               <  1:2393756/244‑1 (MQ=255)

                                                                                         tttATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATAc                                                                                                                                                                                                                                         >  2:1234820/1‑170 (MQ=255)

                                                                                               cgcCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCGTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGgtgt                                                                                                                                                  <  2:1504358/251‑1 (MQ=255)

                                                                                                 ccgaacctgtAGGGCGTCTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAAGGAATATCGCCGACGTGCGTTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCa                                                                                                                                                <  1:73206/242‑1 (MQ=255)

                                                                                                        gTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGAt                                                                                                                                         >  1:102070/1‑251 (MQ=255)

                                                                                                              gCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCCGCAAATGAGTAACCTTCACGATACCGTCATTCATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTTACCCTGAtcacccg                                                                                                                                   >  2:1081061/1‑245 (MQ=255)

                                                                                                              gCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCCCg                                                                                                                                   >  1:1361309/1‑251 (MQ=255)

                                                                                                              gCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCAcn                                                                                                                                   >  1:1444241/1‑250 (MQ=255)

                                                                                                              gCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACg                                                                                                                                   >  1:1694774/1‑251 (MQ=255)

                                                                                                                 gTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTgg                                                                                                                                                                                                   <  1:2302611/184‑1 (MQ=255)

                                                                                                                 gTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATAc                                                                                                                                       >  2:2393974/1‑244 (MQ=255)

                                                                                                                           ccGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTAACGATACCGTCCTTAATCAGGATCATCAGTGCCt                                                                                                                                                                                                              >  1:1382028/1‑163 (MQ=255)

                                                                                                                             gTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCa                                                                                                                                                               <  2:2354449/208‑1 (MQ=255)

                                                                                                                             gTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCa                                                                                                                                     <  2:1573395/234‑1 (MQ=255)

                                                                                                                              tACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCAccc                                                                                                                                             >  2:1882971/1‑225 (MQ=255)

                                                                                                                              tACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGttt                                                                                                                   >  1:301307/1‑251 (MQ=255)

                                                                                                                               aCATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTc                                                                                                                  <  2:40024/251‑1 (MQ=255)

                                                                                                                                cATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATACGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCGTCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCATGCACGATACCAACCGGAATGATGTCACCCTGATACCACGGTTTTTTAAAGAGTTTCt                                                                                                                 >  2:407965/1‑251 (MQ=255)

                                                                                                                                 aTACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAACTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACACTGATACCACGGATTTTTAACGCGTTTCTg                                                                                                                >  2:1011419/1‑251 (MQ=255)

                                                                                                                                           tCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATc                                                                                                                                                                                                                              >  1:736432/1‑131 (MQ=255)

                                                                                                                                           tCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTcg                                                                                                      >  2:1374661/1‑251 (MQ=255)

                                                                                                                                           tCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGATCATCAGCAAATGAGTAACCt                                                                                                                                                                                                                                                  >  2:627054/1‑111 (MQ=255)

                                                                                                                                           tCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACACATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTATTTAATCAGGATCATCTGTGc                                                                                                                                                                                                                >  1:1484897/1‑145 (MQ=255)

                                                                                                                                            cGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATc                                                                                                                                                                                                      <  2:650788/154‑1 (MQ=255)

                                                                                                                                                aGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGt                                                                                                 <  1:1122119/251‑1 (MQ=255)

                                                                                                                                                aGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTCTCTGTTACCATTCGCGGGt                                                                                                 >  2:1586582/1‑251 (MQ=255)

                                                                                                                                                  cTCCCAGTAACCGGAATGAATATCGCCTACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTAATTAATCAGGATCATCAGTGCCTTACTCATCTGGCTTGGGGTCGCTGTCCAGt                                                                                                                                                                                >  1:2008634/1‑170 (MQ=255)

                                                                                                                                                   tCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATc                                                                                                                                                                                                                              >  2:29665/1‑123 (MQ=255)

                                                                                                                                                   tCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTaag                                                                                              >  1:1588608/1‑249 (MQ=255)

                                                                                                                                                   tCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAgg                                                                                              >  1:1720710/1‑251 (MQ=255)

                                                                                                                                                   tCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAgg                                                                                              <  2:1361403/251‑1 (MQ=255)

                                                                                                                                                   tCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTACAGTAACCCTGACCGATACCAACCGGAATGgtgt                                                                                                                                                  >  2:773104/1‑199 (MQ=255)

                                                                                                                                                     ccAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAAt                                                                                                                                                       >  2:737848/1‑192 (MQ=255)

                                                                                                                                                     ccAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCAACCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCa                                                                                            >  1:2154446/1‑251 (MQ=255)

                                                                                                                                                              ggAATGAATATGGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCgg                                                                                   <  1:1045126/251‑1 (MQ=255)

                                                                                                                                                               gaatgaatATCGCCGCCGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGa                                                                                  <  1:2220509/251‑1 (MQ=255)

                                                                                                                                                               gaatgaatATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGa                                                                                  <  2:1713064/251‑1 (MQ=255)

                                                                                                                                                               gaatgaatATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGa                                                                                  <  2:1444347/251‑1 (MQ=255)

                                                                                                                                                                   gaatATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGt                                                                              <  2:958024/251‑1 (MQ=255)

                                                                                                                                                                         cgccgcCGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTcc                                                                        <  2:930377/251‑1 (MQ=255)

                                                                                                                                                                          gCCGGCGGGCGGTTGATGCGGCGGTACCAATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGAAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGCTACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGCACGTTCTTCCg                                                                       <  2:90180/251‑1 (MQ=255)

                                                                                                                                                                           ccGACCGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGc                                                                      <  1:2532147/251‑1 (MQ=255)

                                                                                                                                                                            cGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGcc                                                                     >  1:1573770/1‑251 (MQ=255)

                                                                                                                                                                                gggCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCAGCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGt                                                                 <  2:1681743/251‑1 (MQ=255)

                                                                                                                                                                                  gCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCg                                                               <  2:1822927/251‑1 (MQ=255)

                                                                                                                                                                                  gCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCg                                                               >  2:1689277/1‑251 (MQ=255)

                                                                                                                                                                                      ttCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATAc                                                           >  1:1516232/1‑251 (MQ=255)

                                                                                                                                                                                       tCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATAcc                                                          >  2:242330/1‑251 (MQ=255)

                                                                                                                                                                                           gCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTCCCATTCGCGGGTCGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGgtg                                                      >  1:764352/1‑251 (MQ=255)

                                                                                                                                                                                                             ccTGTTTGCCGTCTTCGGCGGTGCCCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCCACCGGAATGGTGTCACCCTGATACTACGGTTTTTTAAAGCGTTTCTGATTCCATTCGCGGGTCGCCATGTTTCCGTAACGTTCTTCCGCCAGGTCGATACCGGTGTAATgctgtctcttttcc                                    >  2:1023468/1‑238 (MQ=255)

                                                                                                                                                                                                                      cGTCTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACccatc                           <  1:529677/251‑1 (MQ=255)

                                                                                                                                                                                                                         cTTCGGCGGTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGTGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACccatccat                        >  2:1474593/1‑251 (MQ=255)

                                                                                                                                                                                                                             ggcggTGCTCCGCAGCAAATGAGTAACTCTCCCGTTACCGTAATTAATCAGGCTCATCAGGGCCTTAGTCACCTGGATTGGTGTCGCTGTCCAGTATCCCTGACCGTTACCAACCGGAATGGTCTGACCCTGCTACCACGGTTTTTTAAGGCGTTTCTGTTTCCATTTGCGCGTAGGCATGTTGCCGGAACGTTCTTCCGCCCGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCgg                    <  1:1758072/251‑1 (MQ=255)

                                                                                                                                                                                                                             ggcggTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGGAATGACCATAACCGAATTTACCCATCCATTCgg                    <  2:681051/251‑1 (MQ=255)

                                                                                                                                                                                                                                 gTGCTCATCAGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGagag                <  2:2469431/251‑1 (MQ=255)

                                                                                                                                                                                                                                       atcaGCAAATGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGATTGGTGTCACCCTGATACCACGGATTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTCGACATGTTGCCGGACCGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCCTCCATTCGGAGAGGcgatc          >  2:1734081/1‑251 (MQ=255)

                                                                                                                                                                                                                                              aaTGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATcccc   <  1:242312/251‑1 (MQ=255)

                                                                                                                                                                                                                                               aTGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTCCCAATCCATTCGGAGAGGCGATCGATCCCCa  >  1:2108559/1‑251 (MQ=255)

                                                                                                                                                                                                                                               aTGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCa  <  2:1737638/251‑1 (MQ=255)

                                                                                                                                                                                                                                               aTGAGTAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCa  <  2:2154639/251‑1 (MQ=255)

TGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCA  >  NC_000913/666533‑667022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`