mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | gctrim-C13-Redo-5-135_S5_L001_R2_001 | 2,557,115 | 591,032,901 | 100.0% | 231.1 bases | 251 bases | 93.2% |
| errors | gctrim-C13-Redo-5-135_S5_L001_R1_001 | 2,557,336 | 586,108,616 | 100.0% | 229.2 bases | 251 bases | 99.0% |
| total | 5,114,451 | 1,177,141,517 | 100.0% | 230.2 bases | 251 bases | 96.1% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 249.2 | 6.2 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 8399 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 590 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.064 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.74721 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.8 |
| Consensus minimum coverage each strand | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum coverage each strand | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 04:03:34 26 Jun 2016 | 04:05:12 26 Jun 2016 | 1 minute 38 seconds |
| Read alignment to reference genome | 04:05:13 26 Jun 2016 | 04:10:58 26 Jun 2016 | 5 minutes 45 seconds |
| Preprocessing alignments for candidate junction identification | 04:10:58 26 Jun 2016 | 04:13:05 26 Jun 2016 | 2 minutes 7 seconds |
| Preliminary analysis of coverage distribution | 04:13:05 26 Jun 2016 | 04:19:25 26 Jun 2016 | 6 minutes 20 seconds |
| Identifying junction candidates | 04:19:25 26 Jun 2016 | 04:19:27 26 Jun 2016 | 2 seconds |
| Re-alignment to junction candidates | 04:19:27 26 Jun 2016 | 04:20:52 26 Jun 2016 | 1 minute 25 seconds |
| Resolving alignments with junction candidates | 04:20:52 26 Jun 2016 | 04:26:23 26 Jun 2016 | 5 minutes 31 seconds |
| Creating BAM files | 04:26:23 26 Jun 2016 | 04:30:19 26 Jun 2016 | 3 minutes 56 seconds |
| Tabulating error counts | 04:30:19 26 Jun 2016 | 04:36:21 26 Jun 2016 | 6 minutes 2 seconds |
| Re-calibrating base error rates | 04:36:21 26 Jun 2016 | 04:36:22 26 Jun 2016 | 1 second |
| Examining read alignment evidence | 04:36:22 26 Jun 2016 | 05:19:52 26 Jun 2016 | 43 minutes 30 seconds |
| Polymorphism statistics | 05:19:52 26 Jun 2016 | 05:19:53 26 Jun 2016 | 1 second |
| Output | 05:19:53 26 Jun 2016 | 05:20:50 26 Jun 2016 | 57 seconds |
| Total | 1 hour 17 minutes 15 seconds | ||
