New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4309625	157 (1.070)	4 (0.030)	4/448	12.9	2.5%	coding (1356/1533 nt)	alsA	D‑allose ABC transporter ATPase
	?	NC_000913	= 4309649	164 (1.160)					coding (1332/1533 nt)	alsA	D‑allose ABC transporter ATPase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

GTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4309550‑4309625 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttggcgcGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGATGACATGAGCGAAGAGGAGATTATGGCATGGGCTTTACCACAAGAGTAAAAAGCGAAGCGAGCGAGAAGAAACCGTTCAACTTTGCGCTGTTCTGGG  <  NC_000913/4309649‑4309400                                                                                                                                                                                                                                                                                                                                        GTCGCAGACGGTGATAATGTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGA                                                                                                             <  2:244958/219‑1                         TAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGAGGACATGAGCGAAG                                                                                     >  1:244984/1‑219                                   GACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGCGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCCCGATGACATGCGCGAAGAGGAGATTATGGCCTGGGCTTTACCCCAAGAGAAAAAAGCGA                                           >  2:63893/1‑251                                                     TTTCCGTCGTCCGCCAGTGGGCGCGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGATGACATGAGCGAAGAGGAGATTATGGCATGGGCTTTACCACAAGAGTAAAAAGCGAAGCGAGCGAGAAGAAACC                         <  1:63898/251‑1                                                                        GGCGCGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGATGACATGAGCGAAGAGGAGATTATGGCATGGGCTTTACCACAAGAGTAAAAAGCGAAGCGAGCGAGAAGAAACCGTTCAACTTTGCGCTGTTt      >  1:1144463/1‑250                                                                         GCGCGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGATGACATGAGCGAAGAGGAGATTATGGCATGGGCTTTACCACAAGAGTAAAAAGCGAAGCGAGCGAGAAGAAACCGTTCAACTTTGCGCTGTTCT     >  1:1076279/1‑251                                                                          CGCGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGGCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGATGACATGAGCGAAGAGGAGATTATGGCATGGGCTTTACCACAAGAGTAAAAAGCGAAGCGAGCGAGAAGAAACCGTTCAACTTTGCGCTGTTCTG    <  2:1526480/251‑1                                                                           GCGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGATGACATGAGCGAAGAGGAGATTATGGCATGGGCTTTACC                                                            >  2:1183237/1‑194                                                                            CGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGATGACATGAGCGAAGAGGAGATTATGGCATGGGCTTTACCACAAGAGTAAAAAGCGAAGCGAGCGAGAAGAAACCGTTCAACTTTGCGCTGTTCTGGG  <  1:223079/251‑1                                                                                                                                                                                                                                                                                                                                        GTCGCAGACGGTGATAATTTCAGGTAGTTCAGATGACACCATCAGGATGACTTTTCCGTCGTCCGCCAGTTGGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4309550‑4309625 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttggcgcGAAAGCCGAAATTTACAAAGTGATGCGCCAACTGGCGGACGACGGAAAAGTCATCCTGATGGTGTCATCTGAACTACCTGAAATTATCACCGTCTGCGACCGCATCGCCGTGTTCTGCGAAGGACGACTGACGCAAATCCTGACGAATCGCGATGACATGAGCGAAGAGGAGATTATGGCATGGGCTTTACCACAAGAGTAAAAAGCGAAGCGAGCGAGAAGAAACCGTTCAACTTTGCGCTGTTCTGGG  <  NC_000913/4309649‑4309400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.