Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,051	T→G	E346A (GAA→GCA)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,051	0	T	G	100.0%	137.7 / NA	37	E346A (GAA→GCA)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/15);  total (22/15)

ATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCAC  >  NC_000913/3399854‑3400286                                                                                                                                                                                                      |                                                                                                                                                                                                                                            atatAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCAACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAg                                                                                                                                                                                                     >  1:136705/1‑238 (MQ=255)          cGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTgcc                                                                                                                                                                                            >  1:192035/1‑238 (MQ=255)                    tGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACcgcgcg                                                                                                                                                                                 <  2:192035/239‑1 (MQ=255)                         gTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCCTTGCCGCCACcgccggcccc                                                                                                                                                                             >  2:576731/1‑231 (MQ=255)                            cccAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAg                                                                                                                                                                         >  1:233679/1‑239 (MQ=255)                                ggcggcTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCTGCCACCTCGGGCCACCCAGGTCa                                                                                                                                                                     >  2:192493/1‑239 (MQ=255)                                     cTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGt                                                                                                                                                                                                                  >  1:234656/1‑189 (MQ=255)                                     cTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGt                                                                                                                                                                                                                  <  2:234656/189‑1 (MQ=255)                                        gagacAAAAATAAGGCCGAGCGCAACCAGCACGGCCAGAATAGGGCGAATCTGTAGCGACGGGCCACGGCAAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTc                                                                                                                                                              <  2:663772/234‑1 (MQ=255)                                                          aGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGa                                                                                                                                           >  1:550690/1‑239 (MQ=255)                                                                          ccAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCa                                                                                                                           >  2:647677/1‑239 (MQ=255)                                                                                       aaTCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGt                                                                                                               <  1:647677/238‑1 (MQ=255)                                                                                                  aCGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCAcc                                                                                                                                                                                      >  1:15058/1‑156 (MQ=255)                                                                                                  aCGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCAcc                                                                                                                                                                                      <  2:15058/156‑1 (MQ=255)                                                                                                           ggCTAAAATTTGGCTTCATAAGTTATCGGTATTCTCCTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCTTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTgcgc                                                                                          <  1:192493/239‑1 (MQ=255)                                                                                                                       gCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTACCAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTg                                                                               >  2:556950/1‑238 (MQ=255)                                                                                                                                  tATGCTGATTCCCGTAACAGACCAGGCAGGGTAAACGGCCCCTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGcc                                                                    <  2:233679/238‑1 (MQ=255)                                                                                                                                         aTTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCg                                                             <  1:576731/238‑1 (MQ=255)                                                                                                                                                      aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCCCGCTCGGAGATGTCGGAAg                                                >  2:467824/1‑238 (MQ=255)                                                                                                                                                       ccAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGcc                                              >  1:50456/1‑239 (MQ=255)                                                                                                                                                          ggcaggGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGt                                           >  1:508356/1‑239 (MQ=255)                                                                                                                                                                         tgtttacccTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGc                                 >  1:122954/7‑234 (MQ=255)                                                                                                                                                                         cacTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATTATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGccac                                                                                       >  2:658699/1‑180 (MQ=255)                                                                                                                                                                         cacTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATTATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGccac                                                                                       <  1:658699/180‑1 (MQ=255)                                                                                                                                                                           cTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGgaga                                                         >  2:130069/1‑208 (MQ=255)                                                                                                                                                                           cTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGgaga                                                         <  1:130069/208‑1 (MQ=255)                                                                                                                                                                           cTTCCCCTGCCTGCATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAATGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGc                                 >  2:432725/1‑232 (MQ=255)                                                                                                                                                                                        cATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCgcgc                                                                >  1:605743/1‑188 (MQ=255)                                                                                                                                                                                        cATCCGATTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCgcgc                                                                <  2:605743/188‑1 (MQ=255)                                                                                                                                                                                              aTTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACcgcg                                                                                                                                                                                   <  2:528071/67‑1 (MQ=255)                                                                                                                                                                                              aTTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACcgcg                                                                                                                                                                                   >  1:528071/1‑67 (MQ=255)                                                                                                                                                                                              aTTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGt                                                                                                               >  2:13550/1‑135 (MQ=255)                                                                                                                                                                                              aTTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGt                                                                                                               <  1:13550/135‑1 (MQ=255)                                                                                                                                                                                              aTTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCAc                               <  2:196298/215‑1 (MQ=255)                                                                                                                                                                                              aTTACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCAc                               >  1:196298/1‑215 (MQ=255)                                                                                                                                                                                                tACTCTGCGCTGAACAGGTCGCCGCCGTGCATGTCGATc                                                                                                                                                                                                             <  2:25014/39‑1 (MQ=255)                                                                                                                                                                                                   tctGCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTcac  >  1:608755/1‑239 (MQ=255)                                                                                                                                                                                                      |                                                                                                                                                                                                                                            ATATAAGTACGGATTTGACTGAACGTCCCCAGGCGGCTGTCGGCAATAATAATGCCGAGCGCCACCAGCACCGCCAGAATAAGGCGAATCTGTAGCGACGGGCCACGGCTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCAC  >  NC_000913/3399854‑3400286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑