Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,755,429 (T)5→4 intergenic (‑288/‑269) ydhZ ← / → pykF uncharacterized protein/pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,755,4250T.97.6% 957.5 / 14.6 212intergenic (‑284/‑273)ydhZ/pykFuncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base . (79/128);  total (81/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 240 total aligned reads displayed.

GCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTC  >  NC_000913/1755327‑1755519
                                                                                                   |                                                                                              
 cgcAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgtt                                                                                               <  2:4621273/99‑1 (MQ=255)
  gcAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgttt                                                                                              >  1:1220130/1‑99 (MQ=255)
   cAAACAGTGAAGTTTTTTTTTTATCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgtttt                                                                                             >  2:1660809/1‑99 (MQ=255)
   cAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgtttt                                                                                             <  1:1413352/99‑1 (MQ=255)
    aaaCAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgttttg                                                                                            <  2:1285856/99‑2 (MQ=255)
     aaCAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACGAGTTGTATATTTTTTGAAACGCtgttttgt                                                                                           <  1:4519026/99‑3 (MQ=255)
      aCAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgttttgtt                                                                                          <  2:2550851/99‑4 (MQ=255)
      aCAGTGAAGTTTTTGCGTAACCTGTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATAATTTTTGAAACGCtgttttgtt                                                                                          >  1:5258515/1‑96 (MQ=255)
       cAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTTAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAACCGCtgttttgttt                                                                                         <  2:3729901/99‑5 (MQ=255)
        aGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGTAATGcttttttgtttt                                                                                       >  2:2095002/1‑99 (MQ=255)
        aGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGctgttttgtttt                                                                                       <  2:3108023/99‑1 (MQ=255)
        aGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGctgttttgtttt                                                                                       >  2:2350740/1‑99 (MQ=255)
        aGTGAAGTTTTTGCGTAACCTTTTCACTGGAACGTTAAATCTTTGATAACTATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGctgttttgtttt                                                                                       <  2:3477500/99‑1 (MQ=255)
         gTGAAGTTTTTGCGTAACCTTTTCCCTAGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTc                                                                                      >  2:1732349/1‑99 (MQ=255)
          tGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTcc                                                                                     <  1:3616735/99‑1 (MQ=255)
           gAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTTGAAACGCTGTTTTGTTTTcc                                                                                     >  2:4628509/1‑99 (MQ=255)
           gAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCt                                                                                    <  1:4805624/99‑1 (MQ=255)
             aGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCttt                                                                                  <  1:1978100/99‑1 (MQ=255)
              gTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCtttt                                                                                 <  1:726315/99‑1 (MQ=255)
              atttttGCGTAACCCTTTCGCTGGATTGTTTAATGTTTGGTAACAATTTGTTGTCTAACAAGTTGTATATTTTTTGTGACGCTGTTGTGTTGTCCtttt                                                                                 <  2:2920289/98‑1 (MQ=255)
               tttttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTg                                                                                <  2:631031/99‑1 (MQ=255)
               tttttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTg                                                                                <  2:3807327/99‑1 (MQ=255)
               tttttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTg                                                                                <  1:4441575/99‑1 (MQ=255)
                 tttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGa                                                                              <  1:4636235/99‑1 (MQ=255)
                 tttGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGa                                                                              <  2:3558253/99‑1 (MQ=255)
                      gTAACCTTTTCCCTGTAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAAt                                                                         <  1:3156234/99‑1 (MQ=255)
                      gTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTaa                                                                          >  2:314282/1‑99 (MQ=255)
                      gTAACCGTTGCCCTGGAAGGTTAAATGTTTGATAACAATGTATTGTTTAACAAGTTGTATATTATTTGAAACGCTGTTTTGTTTTCCTTTTGGATTaag                                                                         <  2:673533/99‑2 (MQ=255)
                        atccTTTTCCCTCGAACGTGAAATCTTTGGTGTCAATTTATTGTCTAACAAGTTGTATATTCGTTGTAACGCTGTTTTGGTATCCTTTTGGATTAAttt                                                                       <  2:1440209/97‑1 (MQ=255)
                        aaCCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAAttt                                                                       >  1:3952545/1‑99 (MQ=255)
                          ccTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCa                                                                     <  2:5478401/99‑1 (MQ=255)
                            ttttCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGc                                                                   <  2:2963346/99‑1 (MQ=255)
                               tCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGta                                                                <  1:4718718/99‑1 (MQ=255)
                                 ccTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCCGTTTTGTTTTCCTTTTGGATTAATTTCAGCGtata                                                              >  1:1698531/1‑99 (MQ=255)
                                  cTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATaa                                                             >  2:4227345/1‑99 (MQ=255)
                                     gAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgc                                                          <  1:4169965/99‑1 (MQ=255)
                                     gAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgc                                                          >  1:331560/1‑99 (MQ=255)
                                     gAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgc                                                          >  1:2729687/1‑99 (MQ=255)
                                        cGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgcg                                                       >  1:1079123/1‑99 (MQ=255)
                                        cGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgcg                                                       >  2:889523/1‑99 (MQ=255)
                                         gTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgcgc                                                      >  2:4967081/1‑99 (MQ=255)
                                         gTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgcgc                                                      >  1:5488554/1‑99 (MQ=255)
                                              aTCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAAtt                                                 <  1:5410371/99‑1 (MQ=255)
                                              aTCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAAtt                                                 <  1:3859249/99‑1 (MQ=255)
                                               tCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTg                                                <  1:4308939/99‑1 (MQ=255)
                                                cTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGa                                               <  2:1516429/99‑1 (MQ=255)
                                                cTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGa                                               <  2:2643701/99‑1 (MQ=255)
                                                   tGATAACAATTTATTGTCTAACAATTAGTATATTTGTTGAAACGCTGTTTTGTGTTCCTTTAGGATTAATTTCAGAGTATAATACGCGCCAAGTGAgtc                                            <  2:2726663/99‑1 (MQ=255)
                                                    gATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGActct                                           <  1:3027313/99‑1 (MQ=255)
                                                    gATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGActct                                           >  1:2268069/1‑99 (MQ=255)
                                                     aTAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTGTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCtt                                          <  2:5254300/99‑1 (MQ=255)
                                                       aaCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGa                                        <  1:4122398/99‑1 (MQ=255)
                                                       aaCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGa                                        <  2:4517907/99‑1 (MQ=255)
                                                        ccAATTCATTGTCTCACCAATTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGaa                                       <  1:1668420/98‑1 (MQ=255)
                                                        aCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGaa                                       <  2:3590945/99‑1 (MQ=255)
                                                        aCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGaa                                       <  2:5432852/99‑1 (MQ=255)
                                                        aCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGaa                                       <  1:5565992/99‑1 (MQ=255)
                                                         cAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAt                                      >  2:3793384/1‑99 (MQ=255)
                                                          aaTTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATg                                     <  1:3415257/99‑1 (MQ=255)
                                                          aaTTTATTGTCTAACAAGTTGTATATTCTTTGAAACGCTGTTTTGTTTTCATTTTGGATTAATTTGAGCGTATAATGCGCGCAAATTGAGTCTTGAATg                                     <  2:1332135/99‑1 (MQ=255)
                                                           aTTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATgg                                    >  2:4155792/1‑99 (MQ=255)
                                                            tttATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGt                                   >  2:2747999/1‑99 (MQ=255)
                                                              tATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGttt                                 <  1:1855429/99‑1 (MQ=255)
                                                               aTTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTc                                <  2:1603429/99‑1 (MQ=255)
                                                               aTTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGATTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTc                                <  1:4984296/99‑1 (MQ=255)
                                                                ttGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCa                               >  2:2010322/1‑99 (MQ=255)
                                                                 tGTCTAACAAGTTGTATATTTTTTGATACGCTGTTTTGTTTTCCTTTTGTATTAATTTCAGCGTATAATGCGCGCCAATTGGCTCTTGAATGGTTTCAg                              <  2:2884570/99‑1 (MQ=255)
                                                                  gTCTAACAAGTCGTATATTTTTTGAAACGCAGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTAAATGGTTTCAg                              <  1:3564290/99‑1 (MQ=255)
                                                                   tCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCa                            >  1:867484/1‑99 (MQ=255)
                                                                   tCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCa                            >  1:215662/1‑99 (MQ=255)
                                                                     tAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACt                          >  1:1498806/1‑99 (MQ=255)
                                                                     tAACAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACt                          >  1:4661825/1‑99 (MQ=255)
                                                                      aaCAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACtt                         <  1:2565234/99‑1 (MQ=255)
                                                                       aCAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCTCCAATTGACTCTTGAATGGTTTCAGCACttt                        >  2:1229828/1‑99 (MQ=255)
                                                                       aCAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACttt                        <  1:2350725/99‑1 (MQ=255)
                                                                       aCAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACttt                        <  1:3101555/99‑1 (MQ=255)
                                                                       aCAAGTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACttt                        <  2:2218989/99‑1 (MQ=255)
                                                                           gTTGTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGAc                    >  1:3769996/1‑99 (MQ=255)
                                                                              gTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGt                 >  1:1090249/1‑99 (MQ=255)
                                                                              gTATATTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGt                 <  2:3963464/99‑1 (MQ=255)
                                                                               tataTTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGtc                >  2:2478158/1‑98 (MQ=255)
                                                                               tataTTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTa                >  1:1487424/1‑99 (MQ=255)
                                                                               tataTTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTa                <  1:2072788/99‑1 (MQ=255)
                                                                               tataTTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTa                <  1:2676600/99‑1 (MQ=255)
                                                                                atatTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAg               >  2:4866056/1‑99 (MQ=255)
                                                                                  atTTTTTGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGaa             <  1:1126436/99‑1 (MQ=255)
                                                                                    ttttttGAAACGCTGTTTTGTTTTCTCTTGGAATTAATTAGAGCGTTTAATGCGTCTCAATTGACTCTTGAATTTTTCCACCCCTTTGCACTGTAAAAc            >  2:584992/1‑99 (MQ=255)
                                                                                    ttttttGAAACGCTGTTTTGTTTTCCTTTTGGAATAATTTCAGCGTATACTGCGCGCCAATTGCCTCTTGAATGCTTTCAGCACTTTGGACTGTAGAAc            >  2:505832/1‑99 (MQ=255)
                                                                                     tttttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGAGTATAATGCGCGCCAATTGACTCTCGTATGGTTTCAGCACTTTGGACTGTAGAACt           <  2:847252/99‑1 (MQ=255)
                                                                                      ttttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTc          >  1:620090/1‑99 (MQ=255)
                                                                                       tttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCa         <  1:2883320/99‑1 (MQ=255)
                                                                                       tttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCa         >  2:329532/1‑99 (MQ=255)
                                                                                        ttGAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCaa        <  1:1320097/99‑1 (MQ=255)
                                                                                          gAAACGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACg      >  1:3899673/1‑99 (MQ=255)
                                                                                          gAAACGCTGTTTTGTTTTCCATTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCCTGAATGGATTCAGCACTTTGGACAGTAGAAATAAACg      >  2:530881/1‑99 (MQ=255)
                                                                                           gaaCGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTTAGCACTTTGGACTGTAGAACTCAACGa     >  2:3781395/2‑99 (MQ=255)
                                                                                           aaaCGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGa     >  1:5620822/1‑99 (MQ=255)
                                                                                            aaCGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGAc    >  2:3455824/1‑99 (MQ=255)
                                                                                            aaCGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGAc    >  2:1043731/1‑99 (MQ=255)
                                                                                            aaCGCTGTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGAc    >  2:3692625/1‑99 (MQ=255)
                                                                                                   |                                                                                              
GCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTC  >  NC_000913/1755327‑1755519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: