Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,510,524 C→A intergenic (+391/+166) yjhV → / ← fecE pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,510,5240CA95.5% 660.5 / 10.2 204intergenic (+391/+166)yjhV/fecEpseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (5/4);  new base A (89/104);  total (95/109)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

TGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGT  >  NC_000913/4510428‑4510621
                                                                                                |                                                                                                 
tGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGatc                                                                                                 <  2:1769978/99‑1 (MQ=255)
tGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGatc                                                                                                 <  2:2824815/99‑1 (MQ=255)
tGCCGTAAGGGTTAGACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGatc                                                                                                 <  1:2016306/99‑1 (MQ=255)
   cGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTgg                                                                                              <  2:1162921/99‑1 (MQ=255)
       aGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTg                                                                                          >  2:755216/1‑99 (MQ=255)
        gggTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGc                                                                                         >  2:2714336/1‑99 (MQ=255)
          gTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGt                                                                                       <  1:580673/99‑1 (MQ=255)
             ataCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGa                                                                                    <  1:2231024/99‑1 (MQ=255)
             ataCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGa                                                                                    <  1:2906144/99‑1 (MQ=255)
              taCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGAt                                                                                   <  2:1392732/99‑1 (MQ=255)
              taCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGAt                                                                                   <  2:5005011/99‑1 (MQ=255)
              taCAGAAATGTCCGTTAAACCGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGGTTGCGTTGAt                                                                                   >  1:4806576/1‑99 (MQ=255)
               aCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGAtt                                                                                  >  1:5542069/1‑99 (MQ=255)
               aCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGAtt                                                                                  <  2:327075/99‑1 (MQ=255)
               aCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGAtt                                                                                  <  2:641601/99‑1 (MQ=255)
                cAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTa                                                                                 <  1:2070894/99‑1 (MQ=255)
                 aGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTAt                                                                                >  1:602340/1‑99 (MQ=255)
                 aGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTAt                                                                                >  1:4556281/1‑99 (MQ=255)
                 aGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTAt                                                                                >  1:4327186/1‑99 (MQ=255)
                 aGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTAt                                                                                >  2:1709074/1‑99 (MQ=255)
                   aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGa                                                                              <  1:4839812/99‑1 (MQ=255)
                   aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGa                                                                              <  1:3647569/99‑1 (MQ=255)
                     aTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAAc                                                                            >  2:4800948/1‑99 (MQ=255)
                     aTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAAc                                                                            >  2:3130747/1‑99 (MQ=255)
                     aTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAAc                                                                            <  2:1311874/99‑1 (MQ=255)
                       gTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCGAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCt                                                                          <  2:5426697/99‑1 (MQ=255)
                       gTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCt                                                                          <  1:771818/99‑1 (MQ=255)
                       gTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCt                                                                          >  1:2125528/1‑99 (MQ=255)
                        tCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGTGCCAAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTg                                                                         >  2:2338191/1‑99 (MQ=255)
                         ccGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGt                                                                        >  2:1848285/1‑99 (MQ=255)
                         ccGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGt                                                                        <  2:60770/99‑1 (MQ=255)
                          cGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTc                                                                       >  1:1018173/1‑99 (MQ=255)
                           gtgaaGCAGAGTTCAAAATTGATTGCCGTGACCACGACTGGTTTGAAAGCGGCGCCCACGCCCGTACAGATCTGGTTTGCGTTGATTATGATCCTGTCa                                                                      <  2:2327012/96‑1 (MQ=255)
                           gTTAAGCAGATTTCAAAATTGATTGCCGTGATCACGACTGTTTTGAAAGCCTCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCa                                                                      <  2:2281850/99‑1 (MQ=255)
                           gTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCa                                                                      <  1:5366444/99‑1 (MQ=255)
                           gTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCa                                                                      >  1:1791329/1‑99 (MQ=255)
                           gTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCa                                                                      <  2:3395026/99‑1 (MQ=255)
                                gCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTa                                                                 <  1:1959609/99‑1 (MQ=255)
                                gCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTa                                                                 >  1:166279/1‑99 (MQ=255)
                                gCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTa                                                                 >  2:4923522/1‑99 (MQ=255)
                                 cAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTaa                                                                >  2:1454071/1‑99 (MQ=255)
                                 cAGAGGTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTaa                                                                >  2:815531/1‑99 (MQ=255)
                                  agagTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTaaa                                                               <  1:4575692/99‑1 (MQ=255)
                                    agTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAaagc                                                             <  2:3022775/99‑1 (MQ=255)
                                      ttCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAaagcaa                                                           >  1:959762/1‑99 (MQ=255)
                                      ttCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAaagcaa                                                           >  1:2933532/1‑99 (MQ=255)
                                         aaaaTTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCg                                                        <  1:2431474/99‑1 (MQ=255)
                                          aaaTTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAGCCTGTCAGCCTAAAGCAAGCgg                                                       >  1:5137023/1‑99 (MQ=255)
                                          aaaTTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCgg                                                       >  1:347043/1‑99 (MQ=255)
                                          aaaTTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCgg                                                       >  2:4273016/1‑99 (MQ=255)
                                          aaaTTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCgg                                                       <  1:267073/99‑1 (MQ=255)
                                          aaaTTGATTGCCGTGATCACGACTGCTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCgg                                                       <  1:842573/99‑1 (MQ=255)
                                              tgattgCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATgg                                                   >  1:4276295/1‑99 (MQ=255)
                                                   gCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCATTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGAt                                              <  1:3270330/99‑1 (MQ=255)
                                                    ccGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATg                                             >  2:3615360/1‑99 (MQ=255)
                                                    ccGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATg                                             >  1:3079111/1‑99 (MQ=255)
                                                    ccGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATg                                             >  2:2994417/1‑99 (MQ=255)
                                                     cGTGATCACGACTGGTTTGAAAGCCGCGCCTAAGCCTGTACAGATCTGCTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGa                                            <  2:4997403/99‑1 (MQ=255)
                                                     cGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGa                                            <  2:4047854/99‑1 (MQ=255)
                                                      gTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAg                                           >  2:2038262/1‑99 (MQ=255)
                                                         ataaCGACTGGTTTGAATGCCGCGCCCAAGCCTGGAGAGGTCGGGTTTGCGTTGATTATGAACCGGTCAGCCTAACGCAGGCGGAGGGACGATGAGGAt                                        >  2:124121/4‑99 (MQ=255)
                                                         aTCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTAt                                        <  2:458286/99‑1 (MQ=255)
                                                         aTCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTAt                                        >  1:3787606/1‑99 (MQ=255)
                                                           cACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTg                                      <  1:5178840/99‑1 (MQ=255)
                                                           cACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTg                                      <  1:38790/99‑1 (MQ=255)
                                                           cACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTg                                      <  1:3421431/99‑1 (MQ=255)
                                                              gACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTa                                   >  1:5259985/1‑99 (MQ=255)
                                                              aaCTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTa                                   >  2:2000357/2‑99 (MQ=255)
                                                               aCTGGTTTGAACGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGTTAGCGGATGGACGAGGAGTATTGGTaa                                  <  1:4963809/99‑1 (MQ=255)
                                                               aCTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTaa                                  <  1:2479363/99‑1 (MQ=255)
                                                                cTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAAt                                 >  2:2897612/1‑99 (MQ=255)
                                                                 tGGTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATc                                >  2:3936397/1‑99 (MQ=255)
                                                                  ggTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCt                               <  1:3515810/99‑1 (MQ=255)
                                                                   gTTTGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCtt                              >  2:2132741/1‑99 (MQ=255)
                                                                    tttGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTCTTGGTAATCttt                             >  2:4333087/1‑99 (MQ=255)
                                                                     ttGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTc                            <  2:1010478/99‑1 (MQ=255)
                                                                     ttGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTc                            >  2:4067369/1‑99 (MQ=255)
                                                                      tGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCa                           <  1:5440540/99‑1 (MQ=255)
                                                                      tGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCa                           <  2:2583211/99‑1 (MQ=255)
                                                                      tGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCa                           <  1:4413972/99‑1 (MQ=255)
                                                                      tGAAAGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCa                           <  1:4218056/99‑1 (MQ=255)
                                                                        aaaGCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCaga                         >  1:371431/1‑99 (MQ=255)
                                                                         aaGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCagag                        >  1:1156316/1‑99 (MQ=255)
                                                                           gCCGCGCCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTc                      >  1:3157980/1‑99 (MQ=255)
                                                                             cgtgcCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCg                    <  1:2379630/96‑1 (MQ=255)
                                                                              gcgcCCAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCgg                   >  1:409357/1‑99 (MQ=255)
                                                                                 cccAAGCCTGTACAGATCTGTTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATTAGTATTGGTAATCTTTCAGAGTCCGGaaa                <  2:1035561/99‑1 (MQ=255)
                                                                                  ccAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGaaaa               >  2:4866946/1‑99 (MQ=255)
                                                                                   cAAGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAg              <  2:5211648/99‑1 (MQ=255)
                                                                                    aaGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGt             >  1:3240338/1‑99 (MQ=255)
                                                                                    aaGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGt             >  2:3441801/1‑99 (MQ=255)
                                                                                     aGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGtt            >  2:1082664/1‑99 (MQ=255)
                                                                                     aGCCTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGtt            >  2:1744998/1‑99 (MQ=255)
                                                                                       ccTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCa          >  2:2534655/1‑99 (MQ=255)
                                                                                        cTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAg         >  1:4726234/1‑99 (MQ=255)
                                                                                        cTGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAg         >  1:3012888/1‑99 (MQ=255)
                                                                                         tGTACAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGc        >  1:1809342/1‑99 (MQ=255)
                                                                                             cAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAATCAAGCGGAGAGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCa    >  2:572619/1‑99 (MQ=255)
                                                                                             cAGATCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCa    <  2:472910/99‑1 (MQ=255)
                                                                                               gCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGt  <  1:2571848/99‑1 (MQ=255)
                                                                                                |                                                                                                 
TGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCCCAGT  >  NC_000913/4510428‑4510621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: