Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,182,881 A→T E546V (GAA→GTA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,8810AT95.9% 645.3 / 10.4 197E546V (GAA→GTA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (6/2);  new base T (100/88);  total (107/90)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

ATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTG  >  NC_000913/4182787‑4182975
                                                                                              |                                                                                              
aTGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGtt                                                                                            >  1:453530/1‑99 (MQ=255)
aTGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGtt                                                                                            >  2:3519010/1‑99 (MQ=255)
aTGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGtt                                                                                            >  1:1367505/1‑99 (MQ=255)
 tGGACCAGAACAAGCCGCTGGCTGAGAGTACGCGCAAAGGTCGTAGCTGCGCGCGCGGCCGAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGtgc                                                                                           <  2:1328908/99‑3 (MQ=255)
 tGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTc                                                                                           <  1:209705/99‑1 (MQ=255)
 tGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTc                                                                                           <  1:349308/99‑1 (MQ=255)
 tGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCAGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGCAGTTc                                                                                           >  1:3510661/1‑99 (MQ=255)
  ggACCAGAACAACCCGCTGTCTGAGATTACGCACAAATGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCg                                                                                          <  1:145009/99‑1 (MQ=255)
    aCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCgag                                                                                        <  1:2496416/99‑1 (MQ=255)
    aCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCgag                                                                                        <  2:2194934/99‑1 (MQ=255)
     ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCgaga                                                                                       <  1:1629353/99‑1 (MQ=255)
       aGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACg                                                                                     >  1:1767867/1‑99 (MQ=255)
         aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTa                                                                                   >  1:800774/1‑99 (MQ=255)
             acCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACAcc                                                                               <  1:980258/99‑1 (MQ=255)
              cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACAccc                                                                              >  1:26220/1‑99 (MQ=255)
               ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCg                                                                             >  1:40953/1‑99 (MQ=255)
                 gCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGAc                                                                           >  2:3658317/1‑99 (MQ=255)
                  cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACt                                                                          <  1:3778857/99‑1 (MQ=255)
                     tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCAc                                                                       >  1:3440679/1‑99 (MQ=255)
                     tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCAc                                                                       >  2:1663498/1‑99 (MQ=255)
                     tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCAc                                                                       >  2:1695637/1‑99 (MQ=255)
                     tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCAc                                                                       >  1:622902/1‑99 (MQ=255)
                     tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCAc                                                                       <  1:2183515/99‑1 (MQ=255)
                     tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCAc                                                                       >  2:2781854/1‑99 (MQ=255)
                       tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTa                                                                     <  2:4156185/99‑1 (MQ=255)
                          gaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACgg                                                                  >  2:2090546/1‑99 (MQ=255)
                          gaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACgg                                                                  <  1:564540/99‑1 (MQ=255)
                           aTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGt                                                                 <  2:1504562/99‑1 (MQ=255)
                            ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTc                                                                <  1:1307975/99‑1 (MQ=255)
                              aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTcgc                                                              <  1:191105/99‑1 (MQ=255)
                              aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTcgc                                                              <  2:3683169/99‑1 (MQ=255)
                               cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGCGACGTACACCCGACTCACTACGGTcgcg                                                             <  2:70432/99‑1 (MQ=255)
                                gCACAAACGCCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGt                                                            >  2:3934069/1‑99 (MQ=255)
                                 cacaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTa                                                           >  2:458526/1‑99 (MQ=255)
                                  acaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTAt                                                          >  1:3159921/1‑99 (MQ=255)
                                  acaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCGTCGTAGTTCGAGACGTACACCCGACTCACTCCCGTCGCGTAt                                                          >  2:2886059/1‑99 (MQ=255)
                                    aaaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGt                                                        <  1:1833685/99‑1 (MQ=255)
                                    aaaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGt                                                        >  1:2901942/1‑99 (MQ=255)
                                         tcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAAt                                                   >  1:532138/1‑99 (MQ=255)
                                         tcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAAt                                                   <  2:935363/99‑1 (MQ=255)
                                         tcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAAt                                                   >  2:1175249/1‑99 (MQ=255)
                                          cgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATc                                                  <  1:2750586/99‑1 (MQ=255)
                                          cgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATc                                                  <  1:2176744/99‑1 (MQ=255)
                                          cgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATc                                                  <  1:43141/99‑1 (MQ=255)
                                           gtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCg                                                 >  1:684036/1‑99 (MQ=255)
                                            tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGa                                                <  2:1626235/99‑1 (MQ=255)
                                             aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaa                                               <  1:286333/99‑1 (MQ=255)
                                             aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGCCGCGTATGTCCAATCGaa                                               >  2:1212258/1‑99 (MQ=255)
                                              tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaaa                                              <  1:2461156/99‑1 (MQ=255)
                                               ctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAc                                             >  1:2327398/1‑99 (MQ=255)
                                                 ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAccc                                           <  2:436057/99‑1 (MQ=255)
                                                 ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAccc                                           <  2:3767967/99‑1 (MQ=255)
                                                  cGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCCCTACGGTCGCGTATGTCCAATCGAAAcccc                                          >  2:316334/1‑99 (MQ=255)
                                                  cGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAcccc                                          >  2:758419/1‑99 (MQ=255)
                                                  cGCACTCGGCCCAGGCGGTCTGACCCGGGAAGGTGTAGGCTTCGTAGTTCGAGACGTACACTCGACTCACTACGGTCGCGTATGTGCAATCGAAAcccc                                          >  2:2349060/1‑99 (MQ=255)
                                                   gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCt                                         >  2:1674002/1‑99 (MQ=255)
                                                   gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACCGTCGCGTATGTCCAATCGAAACCCCt                                         >  2:2243978/1‑99 (MQ=255)
                                                          gcgcAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTc                                  >  2:2122522/4‑99 (MQ=255)
                                                          gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTGCACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTc                                  <  1:4210872/99‑1 (MQ=255)
                                                          gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTc                                  >  2:331452/1‑99 (MQ=255)
                                                           cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc                                 >  2:3534613/1‑99 (MQ=255)
                                                           cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc                                 <  2:3080062/99‑1 (MQ=255)
                                                           cccAGGCGGTCTGACCCGTGAACGTGCAAGCATCGTAGGTCGAGACAGACATACAACTCACTACGGTCACGGATGTACAATCTAAACCCCTGAAGGAcc                                 <  2:2614801/99‑1 (MQ=255)
                                                            ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg                                >  2:2380778/1‑99 (MQ=255)
                                                             cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa                               >  2:1398135/1‑99 (MQ=255)
                                                              aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa                              <  1:3726961/99‑1 (MQ=255)
                                                                gCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACa                            >  1:3057231/1‑99 (MQ=255)
                                                                 cGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAt                           <  1:1454595/99‑1 (MQ=255)
                                                                  ggTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc                          <  1:746598/99‑1 (MQ=255)
                                                                   gTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                         <  1:13976/99‑1 (MQ=255)
                                                                     cTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                       <  1:3549101/99‑1 (MQ=255)
                                                                     cTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                       <  1:1139802/99‑1 (MQ=255)
                                                                      tGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc                      >  1:3311628/1‑99 (MQ=255)
                                                                      tGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc                      <  1:1420283/99‑1 (MQ=255)
                                                                      tGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc                      <  2:2315860/99‑1 (MQ=255)
                                                                        aCCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGGAGGTCCGAACATCGGTCTg                    >  2:357952/1‑99 (MQ=255)
                                                                        aCCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg                    >  1:1496213/1‑99 (MQ=255)
                                                                        aCCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg                    >  1:110861/1‑99 (MQ=255)
                                                                          ccGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAt                  <  1:2663466/99‑1 (MQ=255)
                                                                          ccGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAt                  >  1:2798137/1‑99 (MQ=255)
                                                                          ccGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAt                  >  1:2038211/1‑99 (MQ=255)
                                                                            gTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa                >  1:2231095/1‑99 (MQ=255)
                                                                             tGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCaa               >  1:391865/1‑99 (MQ=255)
                                                                             tGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCaa               <  1:89663/99‑1 (MQ=255)
                                                                             tGAACGTGCAGGCTTCGTACTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAGCCCCTGAAGGTCCGAACATCGGTCTGATCaa               <  1:3868990/99‑1 (MQ=255)
                                                                              gAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAAc              >  2:142485/1‑99 (MQ=255)
                                                                               aaCGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAAct             >  2:1764849/1‑99 (MQ=255)
                                                                                    gccggCTTCGTAGTTAGAGACGTTCACCCGCCTCCTTTCGATCGCGTATGTACAATCGAAACATCTGCGCGTCAGCACATCAGTCTGATCAActctcta        >  2:652957/4‑98 (MQ=255)
                                                                                    gCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg        <  1:489615/99‑1 (MQ=255)
                                                                                    gCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg        <  1:4072437/99‑1 (MQ=255)
                                                                                    gCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg        <  2:1043380/99‑1 (MQ=255)
                                                                                     cAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGt       >  2:1198827/1‑99 (MQ=255)
                                                                                     cAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGt       >  2:2740980/1‑99 (MQ=255)
                                                                                       ggCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc     <  1:2253981/99‑1 (MQ=255)
                                                                                       ggCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTACAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACCCCCTGTcc     >  2:4027225/1‑99 (MQ=255)
                                                                                         cTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt   <  1:3646217/99‑1 (MQ=255)
                                                                                         cTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt   <  2:1138816/99‑1 (MQ=255)
                                                                                         cTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt   >  2:3175014/1‑99 (MQ=255)
                                                                                          ttCGTAGTTCGAGACGTGCACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtg  >  1:1099503/1‑99 (MQ=255)
                                                                                          ttCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtg  >  2:1893660/1‑99 (MQ=255)
                                                                                              |                                                                                              
ATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTG  >  NC_000913/4182787‑4182975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑