BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,181,174	G→A	G252S (GGC→AGC)	ygiC →	ATP‑Grasp family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,181,174	0	G	A	95.2%	444.1 / 5.0	146	G252S (GGC→AGC)	ygiC	ATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand): ref base G (4/3); new base A (62/76); total (67/79)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 146 total aligned reads displayed.

ATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACC  >  NC_000913/3181082‑3181267

aTTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTAc                                                                                         >  2:2926050/1‑99 (MQ=255)

 tttACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACg                                                                                        >  1:1926674/1‑99 (MQ=255)

 tttACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACg                                                                                        >  1:351546/1‑99 (MQ=255)

   tACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgct                                                                                      >  2:2114759/1‑99 (MQ=255)

    aCAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctg                                                                                     >  1:1594153/1‑99 (MQ=255)

    aCAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctg                                                                                     >  1:2956270/1‑99 (MQ=255)

     cAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctgg                                                                                    >  2:2255752/1‑99 (MQ=255)

     cAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctgg                                                                                    <  1:3509266/99‑1 (MQ=255)

     cAGGACCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTCATGTTGCGTGAGATGTTCTCAACCCAGCTGGAGGATGCCAGCGTACgctgg                                                                                    >  1:525617/1‑99 (MQ=255)

       ggATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctggct                                                                                  >  2:3756876/1‑99 (MQ=255)

        gATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctggctg                                                                                 >  1:1024196/1‑99 (MQ=255)

        gATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctggctg                                                                                 <  1:4311244/99‑1 (MQ=255)

        gATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctggctg                                                                                 >  2:2707746/1‑99 (MQ=255)

        gATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctggctg                                                                                 >  1:2692352/1‑99 (MQ=255)

        gATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACgctggctg                                                                                 <  2:751451/99‑1 (MQ=255)

        gATCAGGTAATTTCCAACCTGTTCAAAATGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCACGCTGGAGGATGCAAGCGTACgctggctg                                                                                 <  2:415890/99‑1 (MQ=255)

          tCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGa                                                                               >  2:2851980/1‑99 (MQ=255)

           gcggTAATTTCCGAGCTGATAAAACAGTATCCGTGTGAATTTATGATGCGTGAGGTGTACTCAACCAAGGTGGAGGATGCAGGCGTTCGCTGGCTGaaa                                                                              <  2:1814273/97‑1 (MQ=255)

           cAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGaa                                                                              <  1:3905010/99‑1 (MQ=255)

            aGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAAc                                                                             <  1:1423375/99‑1 (MQ=255)

             ggTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAAcc                                                                            >  1:3220233/1‑99 (MQ=255)

             ggTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAAcc                                                                            >  1:2287390/1‑99 (MQ=255)

                aaTTTCCAACATGTTCAAACGGCATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACGGGc                                                                         <  2:3616875/99‑1 (MQ=255)

                 aTTTCCATCCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCg                                                                        <  2:3740898/99‑1 (MQ=255)

                 aTTTCCAACCTGTTCAAACTGTATCCGTGGAAATTTATGTTGCGTGAGATATTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCg                                                                        >  2:1641740/1‑99 (MQ=255)

                      cAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGaa                                                                   <  2:1275146/99‑1 (MQ=255)

                        aCCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAaga                                                                 <  2:355132/99‑1 (MQ=255)

                          cTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGc                                                               >  1:565238/1‑99 (MQ=255)

                          cTGTTCAAACTGTATCCGCGGGAATTTATGTTGCGTGGGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAGCCGGAGTGGAAGAGc                                                               <  2:1807307/99‑1 (MQ=255)

                           tGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCa                                                              <  1:820510/99‑1 (MQ=255)

                           tGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCa                                                              <  1:3008983/99‑1 (MQ=255)

                            gTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAGGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCAt                                                             <  2:345136/99‑1 (MQ=255)

                            gTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCAt                                                             <  2:126442/99‑1 (MQ=255)

                            gTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCAt                                                             <  2:2267629/99‑1 (MQ=255)

                             ttCAAACTGTATCCGTGGGACTTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGCGGAAGAGCAtt                                                            <  1:202062/99‑1 (MQ=255)

                             ttCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGTAGTACGCAATCGTACGCTGGCTGGAACCGGCGTGGAAGAGCagt                                                            >  2:72287/1‑97 (MQ=255)

                             tgcAAACTGTATCCGGGGGAATTTATGTGGCGTGAGATGTTCTCAACCAAGCGGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCAtt                                                            <  1:2599411/97‑1 (MQ=255)

                               cAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTAt                                                          >  1:1746730/1‑99 (MQ=255)

                               cAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTAt                                                          <  2:2015090/99‑1 (MQ=255)

                                aaaCTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTAtc                                                         >  1:4107404/1‑99 (MQ=255)

                                aaaCTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTAtc                                                         <  2:3038271/99‑1 (MQ=255)

                                 aaCTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTAtct                                                        <  1:3625457/99‑1 (MQ=255)

                                 aaCTGTATCCGTGGGAATCTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTAtct                                                        <  2:172469/99‑1 (MQ=255)

                                  aCTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTAtctc                                                       >  1:2034697/1‑99 (MQ=255)

                                  aCTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTAtctc                                                       <  1:3098668/99‑1 (MQ=255)

                                   cTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTcc                                                      <  1:763915/99‑1 (MQ=255)

                                       aTCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCcaaca                                                  <  2:652125/99‑1 (MQ=255)

                                        tCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCcaacaa                                                 <  2:3656859/99‑1 (MQ=255)

                                         ccGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAg                                                >  2:1966173/1‑99 (MQ=255)

                                          cGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAgg                                               >  1:3825254/1‑99 (MQ=255)

                                            tGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCa                                             >  1:2628273/1‑99 (MQ=255)

                                              ggAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACt                                           <  1:3688430/99‑1 (MQ=255)

                                                aaTTTATGTTGCGTGAGATGTTCTCAACTAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTc                                         <  2:159475/99‑1 (MQ=255)

                                                aaTTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTc                                         >  2:939/1‑99 (MQ=255)

                                                aaTTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTc                                         >  1:4238174/1‑99 (MQ=255)

                                                aaTTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTc                                         >  1:540516/1‑99 (MQ=255)

                                                 aTTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCt                                        >  2:1424339/1‑99 (MQ=255)

                                                   ttATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTAc                                      <  1:1374929/99‑1 (MQ=255)

                                                   ttATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTAc                                      <  2:233928/99‑1 (MQ=255)

                                                    tATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTAcc                                     <  2:2562217/99‑1 (MQ=255)

                                                     aTGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCCACAAGGCACTTCTACCg                                    >  1:3698795/1‑99 (MQ=255)

                                                        ttGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAGCCGGCGTGGAAGAGCATTATCGCCAACAAGGCACTTCTACCGCTa                                 <  2:409255/99‑1 (MQ=255)

                                                        ttGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTa                                 <  1:1420153/99‑1 (MQ=255)

                                                        ttGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTa                                 <  2:4189132/99‑1 (MQ=255)

                                                          gCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACt                               <  2:1804903/99‑1 (MQ=255)

                                                            gTGAGATGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACtgt                             <  2:403728/99‑1 (MQ=255)

                                                              gagaTGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTgg                           <  1:1896424/99‑1 (MQ=255)

                                                              gagaTGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTgg                           <  1:2889047/99‑1 (MQ=255)

                                                              gagaTGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTgg                           <  2:2492354/99‑1 (MQ=255)

                                                              gagaTGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTgg                           <  2:2832521/99‑1 (MQ=255)

                                                               agaTGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTggg                          <  2:3698106/99‑1 (MQ=255)

                                                               agaTGTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTggg                          <  2:3190451/99‑1 (MQ=255)

                                                                 aTGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGgag                        <  1:950/99‑1 (MQ=255)

                                                                   gTTCTCAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGAt                      >  2:2467233/1‑99 (MQ=255)

                                                                     tctcAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGt                    >  1:3154559/1‑99 (MQ=255)

                                                                      ctcAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGtt                   >  1:723510/1‑99 (MQ=255)

                                                                      ctcAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGtt                   <  2:1496054/99‑1 (MQ=255)

                                                                       tcAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTc                  >  2:26432/1‑99 (MQ=255)

                                                                       tcAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTc                  <  2:1728360/99‑1 (MQ=255)

                                                                       tcAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCACTACTGTGGGAGATGTTc                  >  2:1394855/1‑99 (MQ=255)

                                                                        cAACCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTcc                 <  1:1093462/99‑1 (MQ=255)

                                                                         aaCCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTccc                <  1:3626139/99‑1 (MQ=255)

                                                                         aaCCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTccc                <  2:3981169/99‑1 (MQ=255)

                                                                         aaCCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTccc                >  2:4046571/1‑99 (MQ=255)

                                                                         aaCCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTccc                <  1:3348136/99‑1 (MQ=255)

                                                                         aaCCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTccc                >  1:4188141/1‑99 (MQ=255)

                                                                          aCCAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCg               >  1:3077901/1‑99 (MQ=255)

                                                                            cAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGaa             >  1:964882/1‑99 (MQ=255)

                                                                            cAAGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGaa             <  1:891579/99‑1 (MQ=255)

                                                                             aaGCTGTAGGATGCAAGCGTACGCGCTTTCGTTCCGTCGTGGAAGAGGATTATCTCCAACTTGTCACTTCTACCGCCACTGTGGGAGATGTTCCCGCAt            <  2:921006/99‑1 (MQ=255)

                                                                             aaGCTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAAt            >  1:2089721/1‑99 (MQ=255)

                                                                                cTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCAc         <  1:2246731/99‑1 (MQ=255)

                                                                                cTGGAGGATGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCAc         >  1:3794101/1‑99 (MQ=255)

                                                                                   gaggaTGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCg      >  1:2656751/1‑99 (MQ=255)

                                                                                    aggaTGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGa     >  2:866028/1‑99 (MQ=255)

                                                                                     ggaTGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTAGTGTGGCAGATGTTCCCGAATCACCCGaa    >  1:3957915/1‑99 (MQ=255)

                                                                                      gaTGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAAc   <  2:1562062/99‑1 (MQ=255)

                                                                                      gaTGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAAc   <  2:472056/99‑1 (MQ=255)

                                                                                      gaTGCAAGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAAc   <  1:3387990/99‑1 (MQ=255)

                                                                                       aTGCAAGCGTACGATGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTGCTGTGGGAGATGTTCCCGAATCACCCGAAcc  >  2:14259/1‑99 (MQ=255)

ATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTGTATCCGTGGGAATTTATGTTGCGTGAGATGTTCTCAACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACC  >  NC_000913/3181082‑3181267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`