| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,554,000 | 0 | T | G | 38.5% | 146.3 / 15.3 | 97 | Y557S (TAC→TCC) | maeA | malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme |
| Reads supporting (aligned to +/- strand): ref base T (9/50); new base G (37/0); total (47/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-18 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGAC > NC_000913/1553780‑1554233 | cgcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑GAGTTGCGGGATTCGGCTTGCCCGaaaa > 1:2051810/1‑238 (MQ=255) tataCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGGCGCGGGATTTGGCTTGCCCGGAATTAT‑Cggccat > 1:1457440/1‑234 (MQ=255) gTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGGTGCGGTGATCGGCTTTCCCGAAATAATACGTC‑atgg > 2:1759211/1‑233 (MQ=255) aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGGTTCGGCTTGCCCGAAATTAT‑CGTC‑AATGGCCCGt > 1:1541782/1‑239 (MQ=255) aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTTCGCTTTCCCTGAATTTT‑CTTT‑AATggg > 1:859230/1‑234 (MQ=255) aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTCGGCTTGCCAGAAATTAA‑CGTC‑AATGGc > 1:1188501/1‑235 (MQ=255) aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTAGCGGGATTTGGCTTTCCCGAAAATaatgtccatagg > 1:113694/1‑224 (MQ=255) aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑GAGGCGCGGGATTCGGCTTGGCAGAAAATAT‑CGTC‑AATGGCCTgg > 1:1489606/1‑238 (MQ=255) aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGA‑GGAGGTACGGCGG‑TAGTGGCGGGATTCGGGTTGGCCGAAAATAT‑TGTCTAATGGCCGGt > 2:542309/1‑239 (MQ=255) cATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGGCGCGGTATTTGGCTTTCCAGAAATTAA‑CGTC‑AATGGCCTGGTGCAgggg > 1:452016/1‑238 (MQ=255) gatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTCGGCCTGCCAGAAAATAT‑CGTC‑AAAGGGCTGTTTCAGGGCTTCgg > 1:830992/1‑238 (MQ=255) tctttCCTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGTTGTTCTGCATAGCAGGTGAGGCATATGAGATTTATTCGCCACTACCCAGTATGGTTGAG‑ATCTGAAAATGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGACATCCGGCGCTTTCGTCTCGCCAGAAATTAT‑CGAC‑AACGGCCGTCTGCAGGGCtctgcca > 2:808780/4‑233 (MQ=255) ttCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTCGGCTTGCCAGAAATTAT‑CTTC‑AAAGGCCTGTTGCAGGGCTTCGGCAgggg > 1:1924381/1‑239 (MQ=255) cGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTGGCGGGATTCGGGTTTCCAGAAATTAT‑CTGC‑ACAGGCCCGTTTCAGGGCTTTGGGCGAgggt > 2:854826/1‑237 (MQ=255) cGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTCGGCTTGCCGGAAAATAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCTGCcga > 2:195419/1‑235 (MQ=255) cGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTACCGGGATTTGGCTTTGCACAAAATAA‑CGTC‑CATGGCCTGTTTGCGGGGTTCGGCAGCgggt > 2:295712/1‑237 (MQ=255) aacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTCTGCCTGCCAGAAATTAT‑CGTT‑AAAGGACCGGTGCAGGGCTTCGGCGGAGGTTTTTAcc > 1:891644/1‑239 (MQ=255) acTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTGGCGGGATTTGGCTTGCCCGAAAATTT‑CGTC‑AAATGCCTGTTTGCGGGGTTCGGGCGAGGgttttccc > 2:1412390/1‑234 (MQ=255) cTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTTGGCTTTTCAGAAAATTT‑TGTC‑AAAGGGCCGTTGGAGGGCTTCGGGTGAGGgttttcccg > 1:582046/1‑233 (MQ=255) tGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTCGGGTTGGCAGAAATTAT‑CGTC‑AAAGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGc > 1:353484/1‑238 (MQ=255) cAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTTGGCCTGCCCGAAATTTT‑CGTC‑AAATGCCTGTTGCAGGGCTTCGGGAGGGGGTTTTCCCGccc > 1:1669578/1‑237 (MQ=255) aTAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGCCCGTTGCAGGGGTTCGGCAGAGGGTTTTACCGCCACGccct > 1:2122152/1‑234 (MQ=255) aTAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑GAGTCGCGGGATTCCGCGTGCCCGAAAATAT‑TGTC‑AAAAGACTGTTTCAGGGCGTCGGCAGAGGGTTTTACCGcccccccct > 2:1322317/1‑229 (MQ=255) cctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTTGGCTTGGCAGCAAATAT‑CCTC‑CATGGGCTTTTGCCGGGGTTCGGCAGGGGGTTTTCCCGCCACGCCTtgctgctgc > 2:1706851/1‑238 (MQ=255) gtggatgTGCTGCATAGCAGGTGAGGAAAATGATATTTTTTCGCCACTCCCCAGTATGGATGAG‑ATCGAAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTGCACTACGGGGCGCGGGATTAGATGGAGGTAGGGGGG‑TATCCGCGGTATTCGGTTGGCCGGAAATTAT‑CGCA‑AATGGCCTGTTGCAGGGCTTCGGCAGGGTTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGcc < 2:1585738/235‑1 (MQ=255) gTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTCGGGCTGCCAGGAAATAT‑CGTC‑AAAGGCCTGTTGCAGGGGGTTGGCCGGGGGTTTTACCGCCCCGCCCTGCCGCTGCCGCCTTTTTCCCACCGCCAACGCC‑ACTTcgcg > 2:1006996/1‑239 (MQ=255) gCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑GAGTCGCGGGGTTTGGCCTTCCAGCAAATAT‑CGTC‑CATGGGCTGTTTCAAGGCGTTGGGCGAGGGTTTTATCCCCCCCCCCTTCTTCTGCGCCCCTTTTTCAAACGCAAAAGCACATTGC‑CGGgag > 2:174115/1‑238 (MQ=255) ttttCGCCACTACCAAGTATGGAGGAG‑ATCGGAAAAAGGGAGGGGGAAATAGCCCGGTAGCCTTCACTACGGGGCGCAGGTTTAGAGGGAGGTACGGCGG‑TAGTCGGGGTATTCGGCTGGCCAAAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGTTTCGGCAGAGGTTTTCACCGc < 2:345004/172‑1 (MQ=255) ctcaccatgTATGGA‑GAGAATCTGAAAAGGGGAGGGGGAAATAGCCGGGTCGCCTTCCCTCCCGGGCGCAGGCTTGGATGGAGGTACGGCGG‑TAGTCGGGGTTTGCGGCTTGCCAGAAATTGT‑CGTC‑AAGGGCCTGTTGCGGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGt < 2:1226731/231‑1 (MQ=255) tACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTTGGCTTGCCAGAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCACGCCTtgc > 1:345004/1‑174 (MQ=255) ccaaGTTTGGAGAAG‑ATCGGAAAAAGGGAGGGGAAAATGCCCCGGTGCCCTTCACTCCGGGGGGAAGTCTTAGATGGAGGTAGGGCGG‑TGGCCGCGGTATCGGGCTTGCCGGAAATTAG‑CGTA‑ATTGGCCTGTTGCGGGGTTTCGGAAGAGGTTTGAACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGtt < 2:452016/233‑1 (MQ=255) aGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTTTGCTTGTCAGGAATTAT‑CGGC‑AATGGGCTGTTTCAGGGCTTCGGCAGAGGGTTTTACCCCCCCGCCCTTCTTCTGCGCCCTTTTTCCCACCCCAAACGCA‑ATTTCGCGGGGGACTTTTTTACTATCTTTTAGTTCC‑CGcc > 2:1276416/1‑238 (MQ=255) tATGGATGAG‑ATCTGAAAAAGGGAGGGGGAAATACCCCGGTAGCCTTCCCTACCGGGCGCAGGTTTAAATGGAGGTACGGCGG‑TAGTCGCGTTATTCGGTTTCCCAGAATTTAT‑CGTC‑ATTGGCCTGTTGCAGGGTTTCGGCAGAGTTTTTc < 2:1384431/152‑1 (MQ=255) tATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTTGGCTTGCCCGAAATTAT‑TGGC‑AATGGCCTGTTGCAggg > 2:1927940/1‑135 (MQ=255) tATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGGCGCGGGATTCGGCTTGCCAGaa > 1:52048/1‑109 (MQ=255) gaTGGATGAG‑TTCTAAAAAAGGGAGAGGGAAATCGCCCGGTACCCTTCCCTACGGGGCGAAGGTTTAGAGGGAGGTACGGGGT‑TAGTCGGGGTATTCGGCTTGCCAGAAATTTT‑CGTC‑AATGGCCTGTTGCAGGGTTTCGGCAGAGTTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGc < 2:1624038/235‑1 (MQ=255) ggATGAG‑ACTTGAAAAGGGGGGAGGAAAATGGCCGGGTAGCTTTCACTACCGGGCGCGGGCTAAGTTGGGGGTACGGCGG‑TAGTCGCGGTATTGGGCTTGCCAGAAATTAT‑CGTC‑AATGCCCTGTGGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTAcc < 2:1541782/239‑1 (MQ=255) aTGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTTGGCGTGCCCGAAAATAT‑TGTC‑AAAGGCCCGTTTGCGGGGTTCGGCGGAGGGTTTTACCCCCACGCCCTGCTGCCGCGCCCTTTTTCCCACCGCCAAACCC‑ATTTCCCGGGGGACTTTCTGTATATTTTTCTGTTTC‑g > 2:747907/1‑229 (MQ=255) aaagcgaaaaaaGGGAGAGGAAAAACGCCCGGTGCCCTCCACTCCCGGGGCCAGCCTAAGAGGGAGGTCGGGGGG‑TAGCCGGGGTATCCGGCTTGCCAGAAATTTC‑CGTA‑AAGGGCCGGTGGCAGGGCTTCGGAAGAGTTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATAc < 2:1457440/231‑1 (MQ=255) aTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGGCGCGGGATTCGGCTTTCCAGAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCGCCACGCCTtgctg > 1:1648934/1‑159 (MQ=255) aTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑GAGTCGCGGGATTCGGGTTGCCAGAAATTAT‑CGTC‑AATGGGCCGTTGCAGGGGTTCGGGAGAGGGTTTTACCGCCAAGc > 1:1384431/1‑152 (MQ=255) taaaaaaGGGAGAGGGAAATAGCGGGGTGGCCTCCACTCCGGGGCGCAGTTTAAGAGGGGGGTCGGGCGG‑TAGTCGGGTTATTCGGCTTCCCAGAATTTAC‑CGTC‑AATGGCCTGTTGCGGGGTTTCGGAAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGc < 2:2245672/237‑1 (MQ=255) aaaaaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGGACGGCGG‑TAGTCGCGGGATTCGGCTTGTCACAAATTAT‑AGTC‑AAAGGGCCGTTGCGGGGCTTCTGGAGAGGGTTTCACCGCCACGCCTTTCTGCTGCGCCGTTTTTCCCAACGCAAACGCA‑ACTGAGCTGGGTACTTTTTGTATATCTTTCTATTCC‑GGCAGAACCATAACTTcac > 2:646818/1‑235 (MQ=255) aaaaaGGGAGAGGAAAATAGCCGGGTACCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGGCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AAGGGCCTGTTGCAGGGTTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑gg < 1:388755/220‑1 (MQ=255) gggAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑aa < 1:507565/100‑1 (MQ=255) agGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCCGAAATTAT‑CCTC‑AATCGTCTGTTTCAGGGCTTCGGCCGAGGTTTTCACCGCCACGCCCTGCTGCTGCGCCCTTTTGCCAACCGCCAACGCA‑ATTGCGCGGGGGAGTTTCTGAATATCTTTCAGTTTC‑GGCCGTACCATa > 2:388755/1‑220 (MQ=255) gggAAAGAGCCGGGTGCCCTTCACTCCCGGGCGCAGGCTTAGTTGGAGGTCCGGCGG‑TGGTCGCGGTATTCGGCTTGCCAAAAATTAT‑CGTA‑AATGGCCTGTTGCAGGTCTTCGGCAGAGGTTTCCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCa < 2:582046/238‑1 (MQ=255) ggaaaaTAGCCCGTTAGCCTGCACTACCGGGCGCAGGCTTAGAGGGAGGTACGGGGG‑TAGTCGCGGTATTCGGCTTGCCAAAAATTAT‑CGTC‑AATGGCCTGTGGCAGGGCTTCGGCAGAGTTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCa < 2:295573/235‑1 (MQ=255) gAAATGGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGAGGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AAGGGCCTGTTGCAGGGTTTCGGAAGAGTTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑gg < 1:1412390/207‑1 (MQ=255) gAAATCGCCCGGTAGCCTTCCCTACCGGGCGCAGGCTTAGATGGGGGTCGGGGGG‑TAGTCGCGGTTTTCGGCTTGCCAGAAATTTT‑CGTC‑AATGGCCTGTTGCGGGGTTTCGGCAGAGGTTTTCACCGCCa < 2:1648934/131‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGGGG‑TAGTCGCGGTATTCGGCTTGCCAAAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGc < 1:295712/208‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCgg < 2:52048/71‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCgg < 2:1117409/71‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCt < 1:2091591/73‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGaaa < 1:2148315/82‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGt < 2:336938/89‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑aa < 1:1749331/92‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATg < 1:386592/94‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGcc < 1:1927940/97‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGcc < 1:929173/97‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGcc < 1:814716/97‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGc < 2:1799518/96‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGc < 2:862297/96‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGc < 1:1828751/96‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGc < 1:2118083/96‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGCCTGTTGCAg < 2:536317/105‑1 (MQ=255) gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCGTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑aa < 1:1309408/92‑1 (MQ=255) aaaaaaccccGGTAGCCTTCACTCCCGGGCGCAGGTTTAGATGGGGGTACGGCGG‑TGGTCGCGGTATTCGGTTTGCCAGAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCGGCAGAGTTTTTCACCGCCACGCCTTGCTGCTgcg < 1:1648793/139‑1 (MQ=255) aaTAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑GAGGCGCGGGATTTGGCTTGCCAGAAAATTT‑TGTC‑AATGGCCTGTTg > 2:507565/1‑100 (MQ=255) cGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTTGGCCTGCCCGAAAATTT‑CGTC‑AAAGGGCCGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCCTGCTGCCGCGCCCTTTTTCCAACCCCAAAAACA‑ATTTCGCGGGGGACTTTCTTAATATCTTTTCGTTCC‑CGCCGTACCATACCTTTGCCGGTCAGCACCAATGGTGAAt > 2:1630233/1‑236 (MQ=255) tggctttCACTCCCGGGCGCAGGCTTAGAGGGAGGTACGGCGG‑TAGTCGCGGTTTTCGGCTTGCCAAAAATTAT‑CGTC‑AAGGGCTTGTTGCAGGGTTTCGGCAGAGTTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTg < 1:328902/225‑1 (MQ=255) cTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAt < 1:1741843/70‑1 (MQ=255) cGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGTTTCGGCTTGCCCGAAATTAT‑TTTC‑AAAGGCCTGTTGGCGGGGTTTGGCGGAGGGTTTTTCCCCCCCCCCCTGCTGCTGCCCCATTTTTCCCACCCCCAACCCA‑ATTTCGCGGGGGAGTTTTTTTATATCTTTTACTTTC‑CGCCGTACCATACCCTCCCCGCTCCGCACCAAAAGGGTAAACTGCGCCAGCGCTTTACt > 2:269808/1‑239 (MQ=255) gggCGCAGGCTTAGATGGAGGTACGGCGG‑TAGGCGCGGGATTTGGCTTGCCAGAAATTAT‑CGTC‑AATGGc > 2:1741843/1‑70 (MQ=255) ggCGCAGGCTTAGATGGAGGTACGGCGG‑AAGACCCCGGATTCGGCTTGCCCGAAAATAT‑CGGT‑AATGGCCTGTTGCTGGGGTTCGGGAGAGGGTTTTACCGCCCCGCCCTTCTTCTGCGGCCTTTTTTCAACCCCAAACCCA‑ATTGCGCGGGGGAATTTTTTAATATCTTTTATTTCCTGCCGGAACCACACC‑TCCCCGGTCAGCACCCATAGGGAATACTGCGCCAGCGGTTTAcct > 2:504790/1‑236 (MQ=255) gcgcAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAAAAATTAT‑CGTC‑AATGGCCTTTTGCGGGGCTTCGGCAGAGGTTTTCACCGCCACg < 2:1549841/105‑1 (MQ=255) aGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGGATTTGGCCTGCCCGAAAATAT‑CGTC‑CAAGGCCCGTTGCAGGGGGTCGGCAGAGGGTTTTACCCCCCCCCCTTGCTGCTGCGCCATTTTTTCCACCCCAAACGCC‑AATGCGCCGGGGACTTTTTGAATATTTTTTAGTTTC‑GGCCGTACCATACCCTCGCCGTTTCGCCCCAATGGTGGATAACGCGCCAGAGGTTCACTTTCCGAc > 2:853943/1‑239 (MQ=255) gtttAGATGGGGGTACGGCGG‑TAGTCGCGGTATTCGGTTTGCCAAAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCa < 1:1974717/173‑1 (MQ=255) gCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCGGCa < 1:786109/81‑1 (MQ=255) gCTTAGAGGGAGGTACGGGGG‑TAGTCGCGGTATTCGGCTGCCGAGAAATTTT‑CTTC‑AATGCCCTGTTGCGGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTgcg < 2:1775146/112‑1 (MQ=255) tAGGTGGAGGTGCGGCGG‑TAGCCGCGGTATTGGGCTTGCCAGAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCGGAAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAg < 1:1706851/239‑1 (MQ=255) aGATGGAGGTACGGCGG‑TAGTCGCGGGATTCGGCTTTCCAGAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGGTTCGGCAGAGGGTTTTACCGCCCCGCCTtgctgct > 1:1549841/1‑105 (MQ=255) tGGGGGTAGGGGGG‑TGGTCGGGGTTTTCGGTTTGCAAAAAATTAG‑CGTC‑AATGGCCTGTTGCAGGGTTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCAt < 1:1302621/238‑1 (MQ=255) gggAGTTCCGGCGT‑TAGTCCGGTTTTTCGCTTTGCCAGAATTTAT‑CGTC‑AATGGCCTTTTGCAGGGCTTCGGCAGAGTTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCAt < 1:1414832/237‑1 (MQ=255) ggAGGTACGGCGG‑TAGTCGCGGTATTCTGCTTGGCCGAAAATAT‑CCGC‑AAAGGGCCGTTGCCGGGGGTCGGCCGGGGTTTTTACCGCCCCGCCTTTCTTCTTCGCCCATTTTCCAACCCCTAACGCA‑ATTTCGCGGGGGGATTTTTGAAAATTTTTTAGATTC‑CGCCGGACCATACCTTCGCCCTTCCGCACCCATGGTGTAAAATTCGCCCGCGTTTCACTTGCCCAcatcatcata > 2:1733738/1‑237 (MQ=255) aGGTACGGGGG‑TAGTGGCGGTATTCGGCTTGCAAGAAATTAT‑CGTC‑AAGGGCCTGTGGCAGGGTTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTAc < 1:1138621/169‑1 (MQ=255) aGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGCCTGTTGc > 1:171545/1‑59 (MQ=255) aGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGCCTGTTGc < 2:171545/59‑1 (MQ=255) aGGTACGGCGG‑TAGTCGCGGGTTTCGGCTTGTCCGAAATTAT‑CGTC‑AATGGCCTGTTGCCAGGGTTCGGCCGAGGTTTTCACCGCCACGCCTTGTTTCTGCGCCCTTTTGCCCACCGCCAACGCC‑ATTTCGCGGGGGACTTTCTGCATATTTTTCCGTTTC‑GGCCGGAACCTACCCTCGCCGTTCCTCACCAACTGTGCAAACTTCGCCAGCGCTTCCATTTCCGAcctacgcactc > 2:1170807/1‑227 (MQ=255) gtcgggggg‑TAGTCGCGGTATTCGGCTTGCCAAAAATTAT‑CGTC‑AATGGCCTGTGGCAGGGCTTCGGCAGAGTTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCAt < 1:1276416/231‑1 (MQ=255) gTACGGGGG‑TAGTCGGGGTATTCGGCTTGCCAGAAATTAT‑CGTA‑AATGGCCTGTTGCGGGGCTTCGGCAGAGGTTTCCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCAt < 1:195419/238‑1 (MQ=255) ggtgtcgTCGCGGTTTTCGTCTTGCAAGAAATTAT‑CGTC‑AATGGCCTTTTGCAGGGTTTCGGCAAAGTTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCg < 1:646818/229‑1 (MQ=255) g‑TAGTCGCGGTATTCGGCTTGCCAAAAAATAT‑CGGC‑AATGGCCTGTTGTAGGGTTTCGGGCGAGGGTTTCACCGCCACGCCTTTCTTCTGCGCCCTTTTTCCCACCGCCAACCCC‑ATTTTGCGGGGGACCTTCTGTATATTTTTTTATTCC‑GGCCGTTCCATACCtttg > 2:1138621/1‑167 (MQ=255) agtgggggTTTTCGGCTGGCCAGAAATTAT‑CTTC‑AAGGGCGTGTTGCAGGGCTTCGGCGGAGTTTTCCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGt < 1:854826/233‑1 (MQ=255) ggcgGTATTCGGCTTGAGAAAATTTCT‑CCTC‑AGTGGCGTTTTGACGGTTTTCGCCAGGGGTTTACACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTTAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGAc < 2:1294330/238‑1 (MQ=255) ggTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGcc < 1:1635349/35‑1 (MQ=255) ggTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGcc > 2:1635349/1‑35 (MQ=255) | CGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAG‑ATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGG‑TAGTCGCGGTATTCGGCTTGCCAGAAATTAT‑CGTC‑AATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCA‑ATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCC‑GGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGAC > NC_000913/1553780‑1554233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |