Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,287,710	0	G	A	21.7%	141.2 / 10.1	60	intergenic (‑339/‑54)	acs/nrfA	acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base G (36/11);  new base A (0/13);  total (36/24)
Fisher's exact test for biased strand distribution p-value = 4.83e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGA  >  NC_000913/4287499‑4287945                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              ggTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGcaac                                                                                                                                                                                                                  >  2:210652/1‑239 (MQ=255)            aaGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAAt                                                                                                                                                                                                       >  1:31704/1‑239 (MQ=255)             aGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGACAAACAATAAAAA‑‑CCGCCATTGCACCAATGGCGCact                                                                                                                                                                                                       >  2:168300/1‑236 (MQ=255)                     aacaaAAGTTGATTAATTCTATGAGGAACATGCAGTAATGCATGCTGTTAAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTATTTAAC‑ATGAACTTAAAATTGATTAAAGACAACATACACAGGGTGGTTATTTGTTACACATGGGGGCAAGAAATGTCATGACAGTGGAGGTGGGGTTACTGTCGTAAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGaa                                                                                                                                                                                              <  2:267594/239‑1 (MQ=255)                                      tttttGAGAAACATGCATTTTGGCTTGCTGTAAAAAAAGAGAAATATACTGACTAACT‑AAAAGGGGTTTTTTTCAATGCACTTAAAATTGATAAAAAAAAACATCCCAAGTGTGGTTATTTTTAACACATAGGGGCAAGAAATGACATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAAAAAAAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGAt                                                                                                                                                                             <  2:31704/237‑1 (MQ=255)                                                                                    ttcTGACAACCTCAAAGGTGTTTTTTTAC‑ATGCACTTACAATTGATAAAAAAAAACATTCACAGTGTGGTTTTTTTTTACACATGGGGGCGAGAAATGTCATGACAGTGTAGGGGCGGTTACTGTCGTAAAAAACAAAAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATg                                                                                                                                                                                                <  2:661778/172‑1 (MQ=255)                                                                                             aCTCTAAAGTGGTATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACt                                                                                                                      >  2:237360/1‑239 (MQ=255)                                                                                             aCTCTAAAGTGGTATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACt                                                                                                                      >  1:680701/1‑239 (MQ=255)                                                                                              ctctAAAGTGGTATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTAt                                                                                                                                                                                      >  1:661778/1‑174 (MQ=255)                                                                                                         tATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCAc                                                                                                             >  2:545824/1‑236 (MQ=255)                                                                                                         tATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCt                                                                                                          >  1:521108/1‑239 (MQ=255)                                                                                                                    gCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAAtt                                                                                                                                                                                                      >  2:19744/1‑137 (MQ=255)                                                                                                                    gCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAAtt                                                                                                                                                                                                      <  1:19744/137‑1 (MQ=255)                                                                                                                      aCTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGaa                                                                                                                                                                                              <  1:823700/143‑1 (MQ=255)                                                                                                                      aCTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGaa                                                                                                                                                                                              >  2:823700/1‑143 (MQ=255)                                                                                                                                gATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGAtt                                                                                                                                        >  1:181100/1‑187 (MQ=255)                                                                                                                                          aacaTTCACAGTGTGGTTTTTTTTTACAAATGGGGGCGAGAAATGTAATGACAGTGTAGGTGGGGTTACTGTCGTAAAAAAAAAAAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATg                                                                                                                                                                                                <  2:503801/121‑1 (MQ=255)                                                                                                                                                    gtgtGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTAt                                                                                                                                                                                      >  1:503801/1‑121 (MQ=255)                                                                                                                                                     tgtgGTTTTTTTTTACACATAGGGGCGAGAAATGTCATGACAGTGTAGGTGGGTTTACTGTCGTAAAAAACAATAAAAC‑‑CCGCCATTGCAACAATGGCGCAATTCGGAt                                                                                                                                                                                                 <  2:627050/109‑1 (MQ=255)                                                                                                                                                      gtgGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGcc                                                                                                                                                                                                                          <  2:514603/83‑1 (MQ=255)                                                                                                                                                      gtgGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGcc                                                                                                                                                                                                                          >  1:514603/1‑83 (MQ=255)                                                                                                                                                       tgGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCgaag                                                             >  1:326993/1‑239 (MQ=255)                                                                                                                                                        ggTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATg                                                                                                                                                                                                              >  1:367022/1‑93 (MQ=255)                                                                                                                                                            aTTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGa                                                        <  1:237360/239‑1 (MQ=255)                                                                                                                                                               tGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTa                                                                                                                                                                                       >  1:627050/1‑109 (MQ=255)                                                                                                                                                               tGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCtt                                                                                                                                                     >  1:613209/1‑143 (MQ=255)                                                                                                                                                               tGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACAGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGaaa                                                      >  1:170847/1‑238 (MQ=255)                                                                                                                                                               tGTTACACATAGGGACGAGAAATGTAATGACAGTGTGGGTGGGGTTACTTTCGAAAAAAAAAAAAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGAt                                                                                                                                         <  2:691048/155‑1 (MQ=255)                                                                                                                                                                 ttACACATAGGGGCGACAAATGTCATGACAGTGTAGGTGGGGTTACTGTCGTAAAAAACAAAAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGAt                                                                                                                                                                                                 <  2:809557/97‑1 (MQ=255)                                                                                                                                                                 tcacacAGAGGGGAGAAAAGTGTAATGCAAGTGAAGGTGCGTTTACTGTCAAAAAAAACAAAAAAAC‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCt                                                   <  2:680701/237‑1 (MQ=255)                                                                                                                                                                 taacacATGGGGGCGAGAAATGTCATGACGGTGTAGGTGCGGTTACTGTCGTAAAAAACAAAAAAAA‑‑c                                                                                                                                                                                                                              <  2:645215/66‑1 (MQ=255)                                                                                                                                                                   acacATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGaaa                                                      >  1:548090/1‑234 (MQ=255)                                                                                                                                                                      caTAGGGGCGAGAAATGTCATGACAGAGTGGGTGCGGTTACTGTCGTAAAAAACAAAAAAAA‑‑CCGCCATTGCAACAATGgcg                                                                                                                                                                                                           <  2:913359/82‑1 (MQ=255)                                                                                                                                                                       atgggggCGAACAATGTCATGCCGGTGTAGGTGCGGTTACTGTCGTAAAAAACAAAAAAAC‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGaa                                                                                                                                                                                              <  2:478985/91‑1 (MQ=255)                                                                                                                                                                         aGGGGCGAGCAGTTTCAGGACAGTGTGGGGGCGTTAACTGGCGTAAAAAAAAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCg                                           <  1:168300/239‑1 (MQ=255)                                                                                                                                                                         aGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttc                                                                                                                               >  1:691048/1‑155 (MQ=255)                                                                                                                                                                           gggCGAGCAATTTCATGACATTGTAGGTGCGTTTACTGTCGTAAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCg                                           <  1:832203/237‑1 (MQ=255)                                                                                                                                                                           gggCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGca                                                                                                                                                                                                                    >  1:645215/1‑68 (MQ=255)                                                                                                                                                                           gggCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTa                                                                                                                                                                                       >  1:809557/1‑97 (MQ=255)                                                                                                                                                                           gggCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCtt                                                                                                                                                     >  1:520571/1‑131 (MQ=255)                                                                                                                                                                               gAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCgcagca                                      >  1:159023/1‑238 (MQ=255)                                                                                                                                                                               gAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGATCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCCTTGGCCCgcagca                                      >  2:832203/1‑238 (MQ=255)                                                                                                                                                                                aGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGa                                                                                                                                                                                                  >  2:704629/1‑81 (MQ=255)                                                                                                                                                                                aGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGa                                                                                                                                                                                                  <  1:704629/81‑1 (MQ=255)                                                                                                                                                                                aGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGAt                                                                                                                                                                                                 >  1:913359/1‑82 (MQ=255)                                                                                                                                                                                aGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCAt                                     >  1:313308/1‑238 (MQ=255)                                                                                                                                                                                 gCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttc                                                                                                                               >  2:255403/1‑147 (MQ=255)                                                                                                                                                                                 gCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttc                                                                                                                               <  1:255403/147‑1 (MQ=255)                                                                                                                                                                                 gCAATGTCATGACAGCGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGa                                                                                                                                                                                    >  1:478985/1‑94 (MQ=255)                                                                                                                                                                                                ttgtggggctgTTACTGGCGAAAAAAAAAAAAAACC‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAatat                         <  2:326993/224‑1 (MQ=255)                                                                                                                                                                                                tgtAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAg                                                                                                                                                                                             <  1:1014585/70‑1 (MQ=255)                                                                                                                                                                                                tgtAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAg                                                                                                                                                                                             >  2:1014585/1‑70 (MQ=255)                                                                                                                                                                                                tgtAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAg                                                                                                                                                  <  1:345473/113‑1 (MQ=255)                                                                                                                                                                                                tgtAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAg                                                                                                                                                  >  2:345473/1‑113 (MQ=255)                                                                                                                                                                                                tgtAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGt                                                                             >  2:533907/1‑182 (MQ=255)                                                                                                                                                                                                tgtAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGt                                                                             <  1:533907/182‑1 (MQ=255)                                                                                                                                                                                                          gTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTATTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGaaa                                                      >  1:706132/1‑195 (MQ=255)                                                                                                                                                                                                           gtttcgtgaggAAAAACAAAAAAAAACCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAg            <  2:159023/228‑1 (MQ=255)                                                                                                                                                                                                            tACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACa                                                                                                                                                                                  >  2:493582/1‑69 (MQ=255)                                                                                                                                                                                                            tACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACa                                                                                                                                                                                  <  1:493582/69‑1 (MQ=255)                                                                                                                                                                                                                   gTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTTTCCTGGAAAGCCACCTCGGa  >  1:287492/1‑238 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              GGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTAC‑ATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAA‑‑CCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGA  >  NC_000913/4287499‑4287945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑