Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 380,021 (G)10→8 intergenic (‑140/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0130G.98.3% 273.9 / ‑1.9 60intergenic (‑132/+56)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (29/30);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
*NC_000913380,0140G.96.7% 267.0 / 2.4 60intergenic (‑133/+55)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base . (29/29);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

AGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGA  >  NC_000913/379915‑380105
                                                                                                  ||                                                                                           
aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                                                                             <  1:2051589/100‑1 (MQ=255)
  gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                                           <  1:2317940/100‑1 (MQ=255)
   ggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggg                                                                                          >  2:1439123/1‑100 (MQ=255)
      gTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                                                                       >  2:1571045/1‑100 (MQ=255)
       tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggc                                                                                      <  2:1620575/100‑2 (MQ=255)
        aTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggct                                                                                     <  1:1697081/100‑3 (MQ=255)
        aTTCTATATGTCAATGCATACCCCCCTAACGTATATAACACTGGAGAATAAAATTTCTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggcn                                                                                     <  1:2114206/100‑3 (MQ=255)
           cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaat                                                                                  >  1:2011760/1‑95 (MQ=255)
           cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaat                                                                                  >  2:215229/1‑95 (MQ=255)
              tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCgg                                                                             <  1:2480798/100‑1 (MQ=255)
               atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGc                                                                            <  1:332876/100‑1 (MQ=255)
                tGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCa                                                                           <  1:1461368/100‑1 (MQ=255)
                    aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGa                                                                       >  2:1214173/1‑100 (MQ=255)
                      tGCATACCCCCCTATATTACATAACACTGGAGAATAAAATTTATCCGGTTAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAg                                                                     <  2:966506/100‑1 (MQ=255)
                        cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGgc                                                                   >  2:2154876/1‑100 (MQ=255)
                           aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGgccgc                                                                >  1:1263859/1‑100 (MQ=255)
                            ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                               >  1:979541/1‑100 (MQ=255)
                            ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGACGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGAAAGaccgca                                                               >  2:1074200/1‑99 (MQ=255)
                               cccTATAGTATATAACACTGGGGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                            <  1:1550355/100‑1 (MQ=255)
                               cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                            <  2:1067790/100‑1 (MQ=255)
                               cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                            <  1:2053057/100‑1 (MQ=255)
                                  tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGTAGGCCGCCCCGGAt                                                         >  2:1996252/1‑100 (MQ=255)
                                  tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAt                                                         >  1:2489695/1‑100 (MQ=255)
                                     aGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                      <  2:666548/100‑1 (MQ=255)
                                     aGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                      <  2:334486/100‑1 (MQ=255)
                                      gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                      <  2:1203435/100‑1 (MQ=255)
                                      gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                     >  1:108635/1‑100 (MQ=255)
                                       tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                    <  2:1533525/100‑1 (MQ=255)
                                        atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                   >  2:1808806/1‑100 (MQ=255)
                                         tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGCTAgcgggc                                                  <  2:2487661/100‑1 (MQ=255)
                                         tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc                                                  <  1:1071229/100‑1 (MQ=255)
                                           taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                >  2:682031/1‑100 (MQ=255)
                                           taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                >  2:235045/1‑100 (MQ=255)
                                           taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                <  1:1930168/100‑1 (MQ=255)
                                            aaCACTGGAGAATAAAATTTATCCGGTGACTGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                               <  1:1189523/100‑1 (MQ=255)
                                            aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                               >  2:6788/1‑100 (MQ=255)
                                             acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                              >  2:2062015/1‑100 (MQ=255)
                                                cTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                           >  2:946460/1‑100 (MQ=255)
                                                   gagaAGAAGATTGAGGCGGTGAAGGTGGTCGGGAAAAAGCGGGGGAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                          <  1:1411395/100‑1 (MQ=255)
                                                       aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                    <  1:918663/100‑1 (MQ=255)
                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                   >  1:1001956/1‑100 (MQ=255)
                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                   <  1:2244114/100‑1 (MQ=255)
                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                   >  1:1544639/1‑100 (MQ=255)
                                                        tAAAATTTATCCGGTGAATGCGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                   >  1:2060575/1‑100 (MQ=255)
                                                         aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                  >  2:1225490/1‑100 (MQ=255)
                                                          aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                 >  1:825969/1‑100 (MQ=255)
                                                           aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                >  2:837028/1‑100 (MQ=255)
                                                           aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATAGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGagt                                >  2:2090510/1‑98 (MQ=255)
                                                             tttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAttt                              >  1:2642295/1‑100 (MQ=255)
                                                               tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                            >  1:1837599/1‑100 (MQ=255)
                                                                aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                           <  1:627805/100‑1 (MQ=255)
                                                                 tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGTAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                          <  1:2022938/100‑1 (MQ=255)
                                                                 tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                          <  1:1855076/100‑1 (MQ=255)
                                                                 tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                          <  1:179002/100‑1 (MQ=255)
                                                                    ggTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGAGGCAGAAGGAATCAGGATTTCCAGGTc                       >  2:1356612/1‑100 (MQ=255)
                                                                      tGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg                     <  2:1764120/100‑1 (MQ=255)
                                                                       gAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                    <  2:1214733/100‑1 (MQ=255)
                                                                         aTGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg                  <  1:1235422/100‑1 (MQ=255)
                                                                            tgGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc               <  2:989433/100‑1 (MQ=255)
                                                                            tgGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc               >  1:1155646/1‑100 (MQ=255)
                                                                             ggTCGGAAAACAAAGAGGATA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt              >  2:2370054/1‑100 (MQ=255)
                                                                             ggTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt              <  2:801630/100‑1 (MQ=255)
                                                                                cGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa           <  1:2063880/100‑1 (MQ=255)
                                                                                  gAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg         <  2:395944/100‑1 (MQ=255)
                                                                                    aaaCAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGGCAGACGGGCTGCCAGtt       >  2:797589/1‑100 (MQ=255)
                                                                                      aCAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc     <  2:1625276/100‑1 (MQ=255)
                                                                                      aCAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc     <  1:2358032/100‑1 (MQ=255)
                                                                                       cAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa    >  2:14262/1‑100 (MQ=255)
                                                                                         aaGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa  >  1:1226256/1‑100 (MQ=255)
                                                                                                  ||                                                                                           
AGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGA  >  NC_000913/379915‑380105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: