Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 638,455 (G)5→6 coding (119/747 nt) dsbG ← thiol:disulfide interchange protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913638,4501.G91.9% 232.3 / 15.5 74coding (124/747 nt)dsbGthiol:disulfide interchange protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base . (2/4);  new base G (37/31);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAG  >  NC_000913/638351‑638548
                                                                                                      |                                                                                                    
cGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATtcctcc                                                                                                       >  2:1432088/1‑100 (MQ=255)
 gTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCg                                                                                                     >  2:759760/1‑100 (MQ=255)
  ttGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCgg                                                                                                    >  1:2044226/1‑100 (MQ=255)
    gTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCgggg                                                                                                 <  1:1271281/100‑1 (MQ=255)
    gTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCgggg                                                                                                 >  2:215489/1‑100 (MQ=255)
      aCATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCgggggg                                                                                               <  2:678046/100‑2 (MQ=255)
        aTGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCggggggca                                                                                             <  1:1225299/100‑4 (MQ=255)
         tGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCggggggcat                                                                                            >  1:1762864/1‑96 (MQ=255)
         tGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCggggggcat                                                                                            >  1:1825809/1‑96 (MQ=255)
            aaCCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGa                                                                                         >  2:1943272/1‑100 (MQ=255)
              ccAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAAt                                                                                       >  1:867647/1‑100 (MQ=255)
              ccAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAAt                                                                                       >  1:867610/1‑100 (MQ=255)
                agagATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGt                                                                                     <  2:1433652/100‑1 (MQ=255)
                  agaTAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGttt                                                                                   >  1:290404/1‑100 (MQ=255)
                   gaTAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCAGATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAAAGtttt                                                                                  >  1:2503199/1‑100 (MQ=255)
                     tAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCCTCGAATATTTtga                                                                                >  1:2584737/1‑100 (MQ=255)
                      aGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTtgat                                                                               >  2:651049/1‑100 (MQ=255)
                       gCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTtgatg                                                                              >  1:1821893/1‑100 (MQ=255)
                        cGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTtgatga                                                                             >  2:1398446/1‑100 (MQ=255)
                         gTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTgatgat                                                                            <  1:2134620/100‑1 (MQ=255)
                           gCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTg                                                                          >  2:419423/1‑100 (MQ=255)
                           gCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTg                                                                          <  2:212689/100‑1 (MQ=255)
                           gCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTg                                                                          <  2:342358/100‑1 (MQ=255)
                            cTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGt                                                                         >  2:306272/1‑100 (MQ=255)
                              tACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTaa                                                                       >  2:124470/1‑100 (MQ=255)
                              tACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTaa                                                                       <  1:1294692/100‑1 (MQ=255)
                               aCCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAAt                                                                      <  1:1452268/100‑1 (MQ=255)
                                ccATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATg                                                                     >  1:1784413/1‑100 (MQ=255)
                                    cTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCt                                                                 >  2:2485387/1‑100 (MQ=255)
                                     tGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTg                                                                >  2:951348/1‑100 (MQ=255)
                                      ggAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGt                                                               >  1:2539228/1‑100 (MQ=255)
                                           cAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTc                                                          <  1:351969/100‑1 (MQ=255)
                                           cAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTc                                                          <  1:2590283/100‑1 (MQ=255)
                                           cAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTc                                                          >  2:1159153/1‑100 (MQ=255)
                                             ggTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTcta                                                        >  2:24053/1‑98 (MQ=255)
                                             ggTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCaa                                                        <  2:775906/100‑1 (MQ=255)
                                             ggTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCaa                                                        >  1:1503653/1‑100 (MQ=255)
                                             ggTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCaa                                                        >  1:2039366/1‑100 (MQ=255)
                                              gTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAAt                                                       <  1:691726/100‑1 (MQ=255)
                                              gTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAAt                                                       >  1:808666/1‑100 (MQ=255)
                                               tAGATGCTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATc                                                      <  2:319684/100‑1 (MQ=255)
                                                aGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCTGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCg                                                     >  1:1463820/1‑100 (MQ=255)
                                                aGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCg                                                     <  1:312596/100‑1 (MQ=255)
                                                    ggTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCttt                                                 >  2:1272297/1‑100 (MQ=255)
                                                      tGACGCCCTTATCCTGATACTTTCCGCGATAACCTTTCATTCCTCCGGGAGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTa                                               >  2:2492915/1‑100 (MQ=255)
                                                      tGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTa                                               >  1:8356/1‑100 (MQ=255)
                                                         tgCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTg                                            <  1:2643733/99‑1 (MQ=255)
                                                         cGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTg                                            <  1:1663156/100‑1 (MQ=255)
                                                         cGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTg                                            <  1:80511/100‑1 (MQ=255)
                                                          gCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTgg                                           <  2:2577151/100‑1 (MQ=255)
                                                           cccATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGTCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGa                                          <  2:1178839/100‑1 (MQ=255)
                                                            ccATATCCTGATACTTTCCGAGATAACCTTTCCTTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAg                                         <  2:922348/100‑1 (MQ=255)
                                                             cATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGc                                        <  2:1162799/100‑1 (MQ=255)
                                                             cATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGc                                        >  1:1709878/1‑100 (MQ=255)
                                                             cATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGc                                        >  1:2563306/1‑100 (MQ=255)
                                                               tatCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAg                                      <  1:1147553/100‑1 (MQ=255)
                                                                atCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAgg                                     <  1:265166/100‑1 (MQ=255)
                                                                 tCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGa                                    <  2:827353/100‑1 (MQ=255)
                                                                 tCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGa                                    <  1:1285455/100‑1 (MQ=255)
                                                                 tCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGa                                    >  1:724286/1‑100 (MQ=255)
                                                                  ccTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGaa                                   <  2:1686737/100‑1 (MQ=255)
                                                                   cTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTTTTTTTTTTTTACTGGAGCAGGAAg                                  >  2:1050478/1‑100 (MQ=255)
                                                                   cTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTTTTTCTTTTTTTCTGGAGCAGGAAg                                  >  2:308510/1‑100 (MQ=255)
                                                                    tGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGt                                 <  1:572078/100‑1 (MQ=255)
                                                                    tGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGt                                 >  2:1650694/1‑100 (MQ=255)
                                                                       tACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGTATGTTTTCATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTcc                              >  2:1907748/1‑100 (MQ=255)
                                                                       tACTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTcc                              <  2:1477946/100‑1 (MQ=255)
                                                                         cTTTCCGAGATAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGCGCAGGAAGTTCctc                            >  2:780552/1‑100 (MQ=255)
                                                                              cGAGATTACCTGTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTCATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGa                       >  2:2504705/1‑100 (MQ=255)
                                                                                agaTAACCTTTCATTCCTGCCGGGGGCATCGAATGTTTTTATGATCGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTCCCTCTGCGAAg                     <  1:2471142/100‑1 (MQ=255)
                                                                                agaTAACCTTTCATTCCTACTGGCGGCATCGAATGTTTTCATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTACCTCTGCGAAg                     <  1:2470827/100‑1 (MQ=255)
                                                                                  aTAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGc                   <  2:1319600/100‑1 (MQ=255)
                                                                                   tAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCg                  <  2:1911770/100‑1 (MQ=255)
                                                                                   tAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCg                  <  2:1911760/100‑1 (MQ=255)
                                                                                   tAACCTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCg                  >  1:1956165/1‑100 (MQ=255)
                                                                                   tAACCTTTCATCCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCg                  >  2:666675/1‑100 (MQ=255)
                                                                                      ccTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTTTTTTTTTTTTACTGGAGAAGGAGGTTCCTCTGCCAAGGCGAtt               >  2:600752/1‑100 (MQ=255)
                                                                                      ccTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCTATTTTTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGAtt               >  2:444389/1‑100 (MQ=255)
                                                                                      ccTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGAtt               <  1:334898/100‑1 (MQ=255)
                                                                                      ccTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGAtt               <  1:335726/100‑1 (MQ=255)
                                                                                      ccTTTCATTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGAtt               <  1:639612/100‑1 (MQ=255)
                                                                                      ccTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTg              <  1:243106/100‑1 (MQ=255)
                                                                                            aTTCCTCCGGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGa         >  1:580192/1‑100 (MQ=255)
                                                                                              tcctccgGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAg       <  2:992062/100‑1 (MQ=255)
                                                                                                   ccGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTTATTTTCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGTTTGCCGGAAGCagag  >  2:871706/1‑100 (MQ=255)
                                                                                                      |                                                                                                    
CGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAG  >  NC_000913/638351‑638548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: