| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,874,193 | (C)5→6 | intergenic (‑11/‑159) | yoaI ← / → yeaL | uncharacterized protein/UPF0756 family putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,874,188 | 1 | . | C | 91.8% | 234.5 / 15.3 | 73 | intergenic (‑6/‑164) | yoaI/yeaL | uncharacterized protein/UPF0756 family putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/6); new base C (27/40); total (27/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
TTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAG > NC_000913/1874090‑1874282 | ttCGATAAGAAGAACGGACAACACCTGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑c < 2:2455241/100‑1 (MQ=255)ttCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑c < 1:925424/100‑1 (MQ=255)ttCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑c < 1:416451/100‑1 (MQ=255) tCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑cc > 1:1952661/1‑100 (MQ=255) gATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑cc‑cc > 2:990009/1‑100 (MQ=255) gaagaaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑Ccggg < 2:1324397/100‑2 (MQ=255) aagaaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑Ccggga < 1:629401/100‑3 (MQ=255) aagaaCGGACAAAACCAGTACAAAAAAAAAAAAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑Ccggga < 2:452610/100‑3 (MQ=255) aagaaCGGACAAAACCAGTACAAAAAAAAAAAAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑Ccggga < 2:1046888/100‑3 (MQ=255) agaaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGgag < 1:540949/100‑1 (MQ=255) agaaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGgag < 1:241501/100‑1 (MQ=255) aaCGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGAt < 1:1958578/100‑1 (MQ=255) cGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAg < 1:606569/100‑1 (MQ=255) ggACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGc > 2:2122845/1‑100 (MQ=255) cAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGt > 1:452317/1‑100 (MQ=255) cAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGt > 1:1826295/1‑100 (MQ=255) aaaaCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGtt < 1:1162646/100‑1 (MQ=255) aaaCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGttt > 2:173582/1‑100 (MQ=255) aaaCCAGTACAACAGAAAAAGAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGttt < 2:776455/100‑1 (MQ=255) taCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGtttt < 2:409216/99‑1 (MQ=255) aCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGtttta > 1:417643/1‑99 (MQ=255) ccAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCa > 2:2438605/1‑100 (MQ=255) cAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCAc < 1:596722/100‑1 (MQ=255) aGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACg < 2:678660/100‑1 (MQ=255) gTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGt < 2:869898/100‑1 (MQ=255) gTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGt < 2:564122/100‑1 (MQ=255) acaacaGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTa < 2:776267/100‑1 (MQ=255) cAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAg < 1:1606943/100‑1 (MQ=255) aaTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGc > 2:1083400/1‑100 (MQ=255) aTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCg < 2:1467110/100‑1 (MQ=255) aTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCg > 1:443199/1‑100 (MQ=255) aTGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCg < 2:1342371/100‑1 (MQ=255) aTGGCAAAAAACGATGACGTGATAACCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCg < 1:2166663/100‑1 (MQ=255) tGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGt > 2:452654/1‑100 (MQ=255) ggCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTg < 1:1342726/100‑1 (MQ=255) cAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGca > 1:204531/1‑100 (MQ=255) cAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGca < 1:947972/100‑1 (MQ=255) aaaaaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGcac < 2:2559772/100‑1 (MQ=255) aaaaaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGcac < 2:2400036/100‑1 (MQ=255) aaaaaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGACATAGCTGTTTTCACGTTATTGTTACCGTCcac > 2:2512628/1‑100 (MQ=255) aaaaaCGATCACGTGATAATCAGTGTCTCGACAAACATTTGAGCGTTCATAGCACG‑CC‑CCTCGGGAGATAGCTGTTTTCACGTTCTTGTTAGCGTGcaca < 1:2300244/100‑1 (MQ=255) aaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACaaa < 1:608994/100‑1 (MQ=255) aaaCGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACaaa > 1:92404/1‑100 (MQ=255) cGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATgg > 2:1310345/1‑100 (MQ=255) aCGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGtt < 1:1569520/100‑1 (MQ=255) cGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGttt > 2:886690/1‑100 (MQ=255) gTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTtg < 2:1266171/100‑1 (MQ=255) gTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTtg < 2:421092/100‑1 (MQ=255) gTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTtg > 1:1677488/1‑100 (MQ=255) taTAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGTGATAGCTGTTTTCACGATATTGTTAGAGCACACTCATCGCAGttgctc < 2:599167/99‑5 (MQ=255) aaTCAGTGTCTCGACAAACATTTGCTCGTCCATAGCATG‑CCACC‑CGGGAGATAGCTGTGTTCACGTTATTGTTAGCGTGCACAAATGGCAGTCTGATGAc < 1:2242022/100‑1 (MQ=255) aaTCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGAc < 1:1683430/100‑1 (MQ=255) aaTCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGAc < 1:736141/100‑1 (MQ=255) aTCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGCTAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACa > 2:2493316/1‑100 (MQ=255) tCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAg < 1:741729/100‑1 (MQ=255) tgtCTCGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCg < 1:160621/100‑1 (MQ=255) tctcGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGcc < 2:91033/100‑1 (MQ=255) ctcGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCt > 1:1467399/1‑100 (MQ=255) cGACAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGa > 2:514843/1‑100 (MQ=255) cAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAttt < 2:105739/100‑1 (MQ=255) cAAACATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAttt > 1:1580241/1‑100 (MQ=255) aaCATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAttttt > 2:786621/1‑100 (MQ=255) aaCATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAttttt < 1:2207091/100‑1 (MQ=255) cATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTa < 1:1724191/100‑1 (MQ=255) cATTTGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTa < 1:1724183/100‑1 (MQ=255) tttGATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATg > 1:1275799/1‑100 (MQ=255) gATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaa > 2:1434726/1‑100 (MQ=255) gATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaa > 2:1414376/1‑100 (MQ=255) gATCGTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaa > 2:98798/1‑100 (MQ=255) gTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaaaaaa > 1:495964/1‑100 (MQ=255) gTTCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCaaaaaa > 1:1175796/1‑100 (MQ=255) ttCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAAc < 1:423224/100‑1 (MQ=255) ttCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAAc < 2:115152/100‑1 (MQ=255) tCATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACg < 1:401564/100‑1 (MQ=255) cATAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGt < 1:1767867/100‑1 (MQ=255) aTAGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTa < 2:1724783/100‑1 (MQ=255) aGCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTaaa < 1:2179403/100‑1 (MQ=255) gCATGCCC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAACAAACGTAAAg > 2:370878/1‑100 (MQ=255) | TTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CC‑CC‑CGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAG > NC_000913/1874090‑1874282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |