Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,090,136 (G)5→6 intergenic (+90/‑56) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1311.G85.6% 309.4 / 50.7 104intergenic (+85/‑61)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base . (6/9);  new base G (41/48);  total (47/57)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGC  >  NC_000913/2090032‑2090225
                                                                                                      |                                                                                                  
catcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                                                                                     >  1:2539436/1‑100 (MQ=255)
    atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                              <  2:2587122/100‑1 (MQ=39)
     tcCTGACTAGTCTTTCCGGCGATGTGTGCTGGAAGACATTAAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggaag                                                                                               <  2:1561503/100‑4 (MQ=255)
      ccTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCGGGGGc                                                                                          <  1:1525339/100‑1 (MQ=38)
       cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCCGGAAGGTGATCTTCCGGGGGc                                                                                          <  1:1763234/100‑1 (MQ=38)
       cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggggc                                                                                          <  2:615390/100‑3 (MQ=255)
       cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggggc                                                                                          >  1:1130800/1‑98 (MQ=255)
           cTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCtttt                                                                                      <  1:2463568/100‑1 (MQ=255)
            tAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCCGGAAGGTGATCTTCCGGGGGCtttct                                                                                     <  1:107040/100‑3 (MQ=37)
            tAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAAAAAGCCCCCCGGAAGGTGATCTTCCGGGGGCtttct                                                                                     <  2:1048865/100‑3 (MQ=25)
             aGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCtttttt                                                                                    <  2:1850490/100‑1 (MQ=255)
              gTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTa                                                                                   <  1:753790/100‑1 (MQ=255)
              gTCTTTCAGGCGATGTCTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCCGGAAGGTGATCTTCCGGGGGCtttctca                                                                                   <  1:2176561/100‑5 (MQ=25)
               tCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCAGGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTAt                                                                                  <  2:1697848/100‑1 (MQ=255)
                cTTTCAGGCGATGTGTGCTGAAAGACATTCAGCTCTTCCAGTGGTTGATGTACGCATGGGAAAGCGCCCGGGAGATCACCTTCCGGCGGGCTTTTTTAtt                                                                                 >  2:1691402/1‑100 (MQ=255)
                     aGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGcgcg                                                                            >  1:2148286/1‑100 (MQ=255)
                        cGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGtt                                                                         <  2:1031379/100‑1 (MQ=255)
                         gATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTg                                                                        <  2:800959/100‑1 (MQ=255)
                          aTGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGa                                                                       <  1:467626/100‑1 (MQ=255)
                           tgtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGAt                                                                      >  1:2572386/1‑100 (MQ=255)
                             tgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                    >  1:1390113/1‑100 (MQ=255)
                              gtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAAc                                                                   >  1:2529696/1‑100 (MQ=255)
                              gtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAAc                                                                   <  2:593916/100‑1 (MQ=255)
                              gtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAAc                                                                   >  1:104104/1‑100 (MQ=255)
                               tgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACg                                                                  >  1:1275480/1‑100 (MQ=255)
                               tgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACg                                                                  <  1:883116/100‑1 (MQ=255)
                                gCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACgg                                                                 >  2:1723064/1‑100 (MQ=255)
                                gCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACgg                                                                 <  1:1845760/100‑1 (MQ=255)
                                gCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACgg                                                                 <  1:760939/100‑1 (MQ=255)
                                   ggAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                              >  2:1643360/1‑100 (MQ=255)
                                   ggAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                              >  2:1956685/1‑100 (MQ=255)
                                    gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCa                                                             <  2:1098666/100‑1 (MQ=255)
                                    gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCa                                                             <  2:2028022/100‑1 (MQ=255)
                                       gACATTCAGATCTTCCGGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGCGGCTTTTTTATTGCGCGGTTGATAACGGGTCAGAc                                                          >  2:515287/1‑100 (MQ=255)
                                         cATTCAGATCTTCCAGTGGTGTATGAATGCAGGTGATAGCCCTCGGAAGATCACATTACGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAg                                                        >  2:1232341/1‑100 (MQ=255)
                                         cATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAg                                                        >  1:436683/1‑100 (MQ=255)
                                          aTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAgg                                                       <  1:626232/100‑1 (MQ=255)
                                           ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGt                                                      >  1:2192347/1‑100 (MQ=255)
                                           ttCAGATCTTCCAGTGGTGCATGAACGCATGAAAAAGCACCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAATGGTTCAGACAGGt                                                      >  2:264878/1‑100 (MQ=255)
                                             cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGAGGCTGTTTTATTGCGCGGTTGATAACGGTTCAGAAAgggct                                                    >  1:2157792/1‑97 (MQ=255)
                                               gATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                  >  1:2081077/1‑100 (MQ=255)
                                               gATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                  >  1:668730/1‑100 (MQ=255)
                                               gATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                  >  1:265155/1‑100 (MQ=255)
                                                 tCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGTGCGGTTGATAACGGTTCAGACAGGTTTAAag                                                <  1:1008984/100‑1 (MQ=255)
                                                 tCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag                                                <  1:1731159/100‑1 (MQ=255)
                                                 tCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag                                                <  1:1731183/100‑1 (MQ=255)
                                                   ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAagag                                              >  1:2151678/1‑100 (MQ=255)
                                                    tCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAgg                                             <  1:1293313/100‑1 (MQ=255)
                                                    tCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAgg                                             <  1:584238/100‑1 (MQ=255)
                                                    tCCAATGGAGCATGAACGAATGAGACGTCACCCGAACGTTCACCTGCCGGGCGGCTTTTTTATTGCGCTGTTGATAACGGTTCAGTGAAGTTTAAagagt                                             <  2:47334/100‑2 (MQ=255)
                                                     ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGATGATCACCTTCCGGCGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGa                                            <  2:2557443/100‑1 (MQ=255)
                                                     ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGa                                            <  2:1091629/100‑1 (MQ=255)
                                                     ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGa                                            <  2:2380907/100‑1 (MQ=255)
                                                     ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGa                                            >  1:798338/1‑100 (MQ=255)
                                                      cAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                           <  1:2487333/100‑1 (MQ=255)
                                                      cAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                           <  1:161639/100‑1 (MQ=255)
                                                         tggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATaa                                        <  1:1949244/100‑1 (MQ=255)
                                                          ggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAAc                                       >  1:494972/1‑100 (MQ=255)
                                                           gtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACa                                      <  2:817302/100‑1 (MQ=255)
                                                           gtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACa                                      <  1:1822594/100‑1 (MQ=255)
                                                           gtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTACTGCGCGGTTGATAACGGTTCAGACAGTTTTAAAGAGAAATAACa                                      >  1:2427145/1‑100 (MQ=255)
                                                           gtgCATGAACGCATGAGAAAGCCCCCGGAAGATAACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACa                                      <  2:817405/100‑1 (MQ=255)
                                                            tgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGGTAACGGTTCAGACAGGTTTAAAGAGGAATAACaa                                     >  2:335561/1‑100 (MQ=255)
                                                               aTGAACGCATGATAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAAt                                  <  2:1672799/100‑1 (MQ=255)
                                                                 gAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCCGACAGGTTTAAAGTGGAATAACAAAATga                                >  2:193626/1‑100 (MQ=255)
                                                                 gAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGTCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATga                                <  1:1695557/100‑1 (MQ=255)
                                                                  aaCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgac                               <  1:2381380/100‑1 (MQ=255)
                                                                  aaCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgac                               <  2:1351473/100‑1 (MQ=255)
                                                                    cGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacag                             <  1:1666084/100‑1 (MQ=255)
                                                                      cATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                           >  2:1657289/1‑100 (MQ=255)
                                                                      cATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                           >  1:285351/1‑100 (MQ=255)
                                                                      cATAAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                           >  1:2036214/1‑100 (MQ=255)
                                                                       aTGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagaca                          <  1:1123516/100‑1 (MQ=255)
                                                                       aTGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagaca                          <  2:357493/100‑1 (MQ=255)
                                                                       aTGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCGTTTTTATTGCGCGGTTGATAACGGGTCAGACAGGTTTAAAGAGGAATAACAAAATgacagaca                          >  2:314772/1‑100 (MQ=255)
                                                                        tGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaa                         <  1:67408/100‑1 (MQ=255)
                                                                         gagaAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaac                        <  1:1681844/100‑1 (MQ=255)
                                                                          agaAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaaca                       >  2:1818703/1‑100 (MQ=255)
                                                                            naaGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACt                     >  1:1118301/2‑100 (MQ=255)
                                                                            aaaGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACt                     <  1:309119/100‑1 (MQ=255)
                                                                              agccccccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCg                   >  1:2484327/4‑100 (MQ=38)
                                                                               gCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGGCAGGTATAAAGAGGAATAACAAAAGTACAGACAACACTCGt                  >  2:1559493/1‑100 (MQ=255)
                                                                                 cccccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                 >  1:1029635/1‑100 (MQ=255)
                                                                                 cacccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                 <  1:457578/98‑1 (MQ=255)
                                                                                   cccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa               <  1:1531564/100‑1 (MQ=255)
                                                                                    ccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc              >  2:2100536/1‑100 (MQ=255)
                                                                                    ccGGAAGATCACCTTCCGGGGGGCTTTTCTATTGCGCGGTTGATCACTGTTCAGACAGGCTTCAAGAGGCATATCAAAATCACAGACAACACTCTTTTAc              >  2:2100130/1‑100 (MQ=255)
                                                                                     cGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACg             <  1:1205951/100‑1 (MQ=255)
                                                                                      ggAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGc            <  1:1577146/100‑1 (MQ=255)
                                                                                       gAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCa           >  2:257764/1‑100 (MQ=255)
                                                                                       gAAGATCACCTTCCGGGGCGCTTTATTATTGCGCGGTTGACAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCa           <  1:864257/100‑1 (MQ=255)
                                                                                         aGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATa         <  1:1004467/100‑1 (MQ=255)
                                                                                         aGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATa         <  2:2277742/100‑1 (MQ=255)
                                                                                           aTCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGc       >  2:138140/1‑100 (MQ=255)
                                                                                           aTCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGc       >  1:767767/1‑100 (MQ=255)
                                                                                           aTCACCTTCCGGGGGGCTTGTTTATTGCGCGGTTGATATCGGGTCAGACGGGTTTAAAGCCGAATAACAACATTACGGACAACTCTCGTTTACGCATAGc       <  2:561553/100‑1 (MQ=255)
                                                                                              aCCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt    <  1:1426280/100‑1 (MQ=255)
                                                                                               ccTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATACCAAAATGACAGACAACACTCGTTTACGCATAGCTATg   <  2:2536792/100‑1 (MQ=255)
                                                                                               ccTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATg   <  1:1289545/100‑1 (MQ=255)
                                                                                                cTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGc  >  2:1393600/1‑100 (MQ=255)
                                                                                                      |                                                                                                  
CATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGC  >  NC_000913/2090032‑2090225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: