Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,101,039 (C)7→8 coding (585/720 nt) yggN ← DUF2884 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,101,0321.C88.5% 289.7 / 36.2 96coding (592/720 nt)yggNDUF2884 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base . (8/3);  new base C (44/41);  total (52/44)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 110 total aligned reads displayed.

GGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAA  >  NC_000913/3100933‑3101123
                                                                                                    |                                                                                                
ggCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAg                                                                                                   <  2:828096/100‑1 (MQ=255)
 gCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTCTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c                                                                                                <  1:2119621/100‑1 (MQ=255)
  cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cc                                                                                              >  1:747451/1‑100 (MQ=255)
  cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cc                                                                                              >  1:746966/1‑100 (MQ=255)
   tttCTTCCGGAGTCACAATGGGGCTACAAACATCTTTGCCAAGCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccc                                                                                            <  2:2106180/98‑1 (MQ=255)
    aTCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccc                                                                                          >  1:1149654/1‑100 (MQ=255)
       ttCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccctccc                                                                                      <  1:2032359/100‑1 (MQ=255)
        tCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccccccc                                                                                     >  1:395088/1‑99 (MQ=255)
        tCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCCTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccccccc                                                                                     <  1:2510586/100‑2 (MQ=255)
         ccAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccn                                                                                    <  1:1544356/100‑3 (MQ=255)
         ccAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccccccca                                                                                    >  1:442541/1‑98 (MQ=255)
          cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccag                                                                                   <  2:2155833/100‑4 (MQ=255)
          cAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccag                                                                                   <  2:2478223/100‑4 (MQ=255)
           agagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTTTTTTTTTTTCTACTCGGTTTGGATTGAGGATTGCAG‑‑CCCGCCCCAgg                                                                                   >  2:1530675/1‑100 (MQ=255)
            gagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCt                                                                                 >  1:885386/1‑100 (MQ=255)
               tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTc                                                                               <  1:2020234/100‑1 (MQ=255)
                cacaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTcc                                                                              >  1:1420701/1‑100 (MQ=255)
                 acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTccc                                                                             <  2:1482139/100‑1 (MQ=255)
                 acaACGCGCCTACACGCATCTTTGCCAAACTGCGGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGCATTGAGGATTGCAGCCCCCCCCAGGCTTccc                                                                             <  2:2393931/100‑1 (MQ=255)
                  caAGGCGGCTACAAAGATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTTAGGATTGCAG‑‑CCACCCCTAGGCtttaca                                                                            >  2:405946/1‑96 (MQ=255)
                  caACGCGGCTACAATCTTCTTTTCCAATCTGCTGGAAATCTTTTTGCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCGCACCAGGCGTCCCa                                                                            <  2:508828/100‑1 (MQ=255)
                  caACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCa                                                                            >  1:437309/1‑100 (MQ=255)
                  caACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCa                                                                            >  1:588520/1‑100 (MQ=255)
                  caACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCACCAGGCTTCCCa                                                                            <  2:508800/100‑1 (MQ=255)
                    aCGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGc                                                                          <  1:652311/100‑1 (MQ=255)
                    aCGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGc                                                                          <  2:173644/100‑1 (MQ=255)
                      gcgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGcac                                                                        <  1:1091283/100‑1 (MQ=255)
                      gcgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGcac                                                                        <  2:1874325/100‑1 (MQ=255)
                      gcgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGcac                                                                        <  1:1041825/100‑1 (MQ=255)
                      gcgGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGcaca                                                                       >  2:320835/1‑100 (MQ=255)
                        ngCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACAt                                                                      >  1:2098269/2‑100 (MQ=255)
                         gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGATTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACAtt                                                                     >  2:427483/1‑100 (MQ=255)
                           tACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCt                                                                   <  1:2438917/100‑1 (MQ=255)
                            aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTg                                                                  >  2:830457/1‑100 (MQ=255)
                             cAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACATTCTg                                                                  >  1:185915/1‑100 (MQ=255)
                             cAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGt                                                                 >  1:742478/1‑100 (MQ=255)
                                aCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAt                                                              <  2:35125/100‑1 (MQ=255)
                                aCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAt                                                              <  2:950457/100‑1 (MQ=255)
                                 cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATg                                                             <  2:2279631/100‑1 (MQ=255)
                                  aTCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATgg                                                            >  2:1749610/1‑100 (MQ=255)
                                   tatctGCCAAACTCCCCGAACTCTTTTTCCTGCCCTTTACCCCCGATCTTGATTGAAGATTGCCGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATggg                                                           <  2:897568/96‑1 (MQ=255)
                                   tCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTTTTTTTTTTTTTTATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCCCATTCTGTAATggg                                                           >  2:901760/1‑100 (MQ=255)
                                   tCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATggg                                                           <  1:1614354/100‑1 (MQ=255)
                                   tCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATggg                                                           <  1:496989/100‑1 (MQ=255)
                                    cTTTGCGAAACTGATGGAAATCTTTTTCCTGCTTTTTCCACTCGTTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                          <  2:1981394/100‑1 (MQ=255)
                                    cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTTAGCATTGCAG‑‑CCGCCCCCAGGCTTCCCCGCACATTCTGTAATGGGt                                                          >  2:752859/1‑100 (MQ=255)
                                     tttGCCAAACTGCTGGCAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGtt                                                         >  2:1186694/1‑100 (MQ=255)
                                     tttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAAAGGGtt                                                         >  1:2499870/1‑100 (MQ=255)
                                       tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAc                                                       >  1:365606/1‑100 (MQ=255)
                                         ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTTTTTTTTTTTTTGATTAAGGATTGCAG‑‑CACCCCCCAGGCTTGCGAGCACATTCTGTAATTGGTTCccg                                                     >  2:1463712/1‑100 (MQ=255)
                                           aaaCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgcc                                                   >  1:307411/1‑100 (MQ=255)
                                           aaaCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgcc                                                   >  1:1058835/1‑100 (MQ=255)
                                             aCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACcgccgc                                                 <  2:2308738/100‑1 (MQ=255)
                                                  tgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTc                                            >  1:1546923/1‑100 (MQ=255)
                                                   ggAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCa                                           <  1:1239146/100‑1 (MQ=255)
                                                      aaTCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGc                                         <  2:1439610/100‑1 (MQ=255)
                                                       aTCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc                                       <  1:919852/100‑1 (MQ=255)
                                                         cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                     <  1:1101214/100‑1 (MQ=255)
                                                              tCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTc                                >  1:1007203/1‑100 (MQ=255)
                                                               ccTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcg                               >  2:789766/1‑100 (MQ=255)
                                                               ccTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcg                               <  1:2135799/100‑1 (MQ=255)
                                                               ccTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcg                               >  2:1045492/1‑100 (MQ=255)
                                                                cTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgc                              >  1:24485/1‑100 (MQ=255)
                                                                 tGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                             >  2:2196497/1‑100 (MQ=255)
                                                                 tGCTTTTTCCACGCGGTGTGGGTTGAGGATTGCGGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                             <  2:490899/100‑1 (MQ=255)
                                                                   cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGcc                           <  2:774781/100‑1 (MQ=255)
                                                                   cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGcc                           <  1:275219/100‑1 (MQ=255)
                                                                    tttttCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGccc                          >  1:904436/1‑100 (MQ=255)
                                                                       ttCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAtt                       >  1:2286042/1‑100 (MQ=255)
                                                                          cACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa                    <  1:107231/100‑1 (MQ=255)
                                                                          cACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCa                    <  1:2376360/100‑1 (MQ=255)
                                                                           aCTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt                   <  1:1484239/100‑1 (MQ=255)
                                                                            cTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt                  >  1:2269474/1‑100 (MQ=255)
                                                                            cTCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAtt                  <  2:5216/100‑1 (MQ=255)
                                                                             tCGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTa                 <  2:2179909/100‑1 (MQ=255)
                                                                              cGGTTTGGATTGAGGATTGCGGCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTa                 <  1:901586/100‑1 (MQ=255)
                                                                              cGGTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa                <  1:1204409/100‑1 (MQ=255)
                                                                                gTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg              <  1:893392/100‑1 (MQ=255)
                                                                                gTTTGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg              <  1:2323904/100‑1 (MQ=255)
                                                                                gTTTGGATTGAGGATTGCAG‑‑GCCGCCCAGGCTTCCCAGCACGTTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGc             >  1:1871951/1‑100 (MQ=255)
                                                                                 tttGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGc             <  1:674179/100‑1 (MQ=255)
                                                                                 tttGGATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGc             <  1:739978/100‑1 (MQ=255)
                                                                                   tGGATTGAGTGTTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTg           <  1:1099915/100‑1 (MQ=255)
                                                                                     gATTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTc         >  2:1086732/1‑100 (MQ=255)
                                                                                      aTTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTcc        >  2:1543284/1‑100 (MQ=255)
                                                                                      aTTGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTcc        >  2:84967/1‑100 (MQ=255)
                                                                                      aTTGAGGATTGCAG‑‑CCCGCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTcc        <  1:1572005/100‑1 (MQ=255)
                                                                                       ttGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt       >  1:2327335/1‑100 (MQ=255)
                                                                                       ttGAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTATGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCTTTAATGCTGTctt       >  2:916582/1‑98 (MQ=255)
                                                                                         gAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGt     >  1:1037805/1‑100 (MQ=255)
                                                                                         gAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGt     >  2:2433337/1‑100 (MQ=255)
                                                                                         gAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGt     >  1:1038156/1‑100 (MQ=255)
                                                                                         gAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGt     >  1:1871859/1‑100 (MQ=255)
                                                                                         gAGGATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCCCCGCTTTTCAGCACCGCTTTCGCGCCCATCTCATTAATGCTGTCCTGt     >  1:1069378/1‑100 (MQ=255)
                                                                                           ggATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAGTGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCGATTTCATTAATGCGGTCCTGTaa   >  1:2303902/1‑100 (MQ=255)
                                                                                           ggATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTaa   >  1:1328489/1‑100 (MQ=255)
                                                                                           ggATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTaa   >  1:2569164/1‑100 (MQ=255)
                                                                                           ggATTGCAGCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTaa   <  1:804128/100‑1 (MQ=255)
                                                                                           ggATTCCAGCCCCCCCCAGGCTTCCCAGCACATTCTGCAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTaa   >  1:2451682/1‑100 (MQ=255)
                                                                                            gATTGCAGCCCCCCCCAGGCGTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTaaa  >  1:2065649/1‑100 (MQ=255)
                                                                                                    |                                                                                                
GGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAA  >  NC_000913/3100933‑3101123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: