Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,000,985	A→G	Y14H (TAT→CAT)	fliZ ←	RpoS antagonist; putative regulator of FliA activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,000,985	0	A	G	100.0%	291.5 / NA	84	Y14H (TAT→CAT)	fliZ	RpoS antagonist; putative regulator of FliA activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/45);  total (39/45)

ATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTAC  >  NC_000913/2000890‑2001080                                                                                                |                                                                                                aTTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGATTAATTTAGGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCgg                                                                                             >  1:2511832/1‑100 (MQ=255) aTTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCgg                                                                                             >  1:2512558/1‑100 (MQ=255)   tCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCt                                                                                           <  2:356296/100‑1 (MQ=255)    cACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCtt                                                                                          <  1:793894/100‑1 (MQ=255)      cTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTaa                                                                                        >  1:1924284/1‑100 (MQ=255)       tATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAAt                                                                                       <  1:1896351/100‑1 (MQ=255)       tATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAAt                                                                                       <  1:2329881/100‑1 (MQ=255)         ttttGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATgg                                                                                     <  1:1615631/100‑1 (MQ=255)            tGCCCTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAAAGGCCg                                                                                  <  1:2368921/100‑1 (MQ=255)              ccGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTc                                                                                <  1:560611/100‑1 (MQ=255)              ccGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTc                                                                                <  2:826087/100‑1 (MQ=255)                 tCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCttt                                                                             >  1:1691533/1‑100 (MQ=255)                  cgcgAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCtttt                                                                            >  1:1475006/1‑100 (MQ=255)                  cgcgAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCtttt                                                                            >  1:731122/1‑100 (MQ=255)                    ngAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCa                                                                          >  1:2106204/2‑100 (MQ=255)                        cTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGtg                                                                      >  2:1089338/1‑100 (MQ=255)                         tAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGtgc                                                                     >  1:1105565/1‑100 (MQ=255)                          aaGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGtgct                                                                    >  1:438781/1‑100 (MQ=255)                                aTGCGATCGAGTAATTTACGGCAATGCGCGCGATGGCTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTTATGGCCGTCTTTTCATTTGCTGCACca                                                              >  2:553934/1‑100 (MQ=255)                                   cGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACcatca                                                           <  1:2288836/100‑1 (MQ=255)                                   cGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACcatca                                                           >  1:2541280/1‑100 (MQ=255)                                     aTCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCAtt                                                         >  1:1323929/1‑100 (MQ=255)                                      tCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTa                                                        <  2:1449786/100‑1 (MQ=255)                                       cGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTaa                                                       <  2:1508767/100‑1 (MQ=255)                                        gAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAg                                                      <  1:1041137/100‑1 (MQ=255)                                        gAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAg                                                      <  1:1563688/100‑1 (MQ=255)                                        gAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAg                                                      <  2:2096226/100‑1 (MQ=255)                                         aGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGa                                                     <  1:1273252/100‑1 (MQ=255)                                          gTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGaa                                                    >  2:2391745/1‑100 (MQ=255)                                            aaTTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACt                                                  >  1:147678/1‑100 (MQ=255)                                             aTTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTc                                                 <  1:1304579/100‑1 (MQ=255)                                              tttACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTcn                                                <  1:2108319/100‑2 (MQ=255)                                              tttACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTcc                                                <  1:775191/100‑1 (MQ=255)                                                tACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTg                                              <  1:2466938/100‑1 (MQ=255)                                                 aCGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTgg                                             <  1:243635/100‑1 (MQ=255)                                                  cGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGt                                            <  1:1533032/100‑1 (MQ=255)                                                  cGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGt                                            <  2:1883098/100‑1 (MQ=255)                                                   ggCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTa                                           >  2:188798/1‑100 (MQ=255)                                                   ggCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTa                                           >  1:96870/1‑100 (MQ=255)                                                    gCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAg                                          <  1:304807/100‑1 (MQ=255)                                                    gCAATGCGCGCAATGGGTCTGGCTGTGGTTAAAGTCTTTAAGATGGCGGCTTAATGGCGGTCTTTTCCGGTGCTGCACCCTCATTAAGAACTCCGGGTAg                                          >  2:2181170/1‑100 (MQ=255)                                                       aTGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTTTTTTTTTTTTCCATCATTAAGAACTCCTGGTAGTCa                                       >  2:1064319/1‑100 (MQ=255)                                                        tGCGCGCGATGGGTCTGGCTGTGTTTAAAGTCTTTAAGGTGTCGGCTTAATGGCCGGCTTTTCAGGTACTGCAGCATCATTAGGAACTCCGGGGCGTCaa                                      >  2:641077/1‑100 (MQ=255)                                                          cgcgcAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAg                                    <  1:1881694/100‑1 (MQ=255)                                                           gcgcAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGt                                   <  1:2043681/100‑1 (MQ=255)                                                            cgcAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGtt                                  <  2:1736956/100‑1 (MQ=255)                                                                aTGGGTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAg                              <  1:167529/100‑1 (MQ=255)                                                                  gtgTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTg                            <  1:2420165/98‑1 (MQ=255)                                                                  gggTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTg                            >  2:308444/1‑100 (MQ=255)                                                                   ggTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGc                           <  1:1799863/100‑1 (MQ=255)                                                                   ggTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGc                           >  1:1720727/1‑100 (MQ=255)                                                                    gTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCg                          >  1:763553/1‑100 (MQ=255)                                                                    gTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCg                          <  2:310965/100‑1 (MQ=255)                                                                    gTCTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCg                          >  2:1476470/1‑100 (MQ=255)                                                                      cTGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGc                        <  1:2363134/100‑1 (MQ=255)                                                                       tGGCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCa                       <  2:19213/100‑1 (MQ=255)                                                                        ggCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGACCTCCTGGTAGTCAAAGTTAAAGTGCGGCAt                      >  2:1222438/1‑100 (MQ=255)                                                                        ggCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCAt                      >  1:1107389/1‑100 (MQ=255)                                                                         gCTGTGTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATCACGAACTCCTGGTCGTCCACGTCAAAATCCGCCAtt                     >  2:2222955/1‑100 (MQ=255)                                                                           tgtgTTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTa                   <  1:655968/100‑1 (MQ=255)                                                                            gtgtTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTAc                  >  1:1784778/1‑100 (MQ=255)                                                                             tgtTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACt                 <  2:989158/100‑1 (MQ=255)                                                                             tgtTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACt                 >  2:1349546/1‑100 (MQ=255)                                                                              gtTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTg                >  2:1545619/1‑100 (MQ=255)                                                                              gtTTAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTg                <  1:166239/100‑1 (MQ=255)                                                                                 tAAAGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACg             >  1:1866695/1‑100 (MQ=255)                                                                                  aaaGTCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGt            <  1:1404374/100‑1 (MQ=255)                                                                                   aaGGCTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGtt           <  1:1561004/100‑1 (MQ=255)                                                                                      tCTTTAAGAGGGCGGCTTAATGGTCGTCTTTTCAGGAGCTGCACCATCAGTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATCTACTGACGTtata        <  2:926080/100‑1 (MQ=255)                                                                                       cTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATaa       >  1:290155/1‑100 (MQ=255)                                                                                       cTTTAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATaa       <  2:490706/100‑1 (MQ=255)                                                                                        tttAAGATGTCTGCTTAATGGCCGTCTTTTCATGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAAc      <  2:381309/100‑1 (MQ=255)                                                                                        tttAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAAc      >  2:1747214/1‑100 (MQ=255)                                                                                        tttAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAAc      <  2:2039959/100‑1 (MQ=255)                                                                                        tttAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAAc      >  2:2439874/1‑100 (MQ=255)                                                                                        tttAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAAc      >  1:670868/1‑100 (MQ=255)                                                                                         ttAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACt     >  2:1722227/1‑100 (MQ=255)                                                                                         ttAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACt     >  2:258406/1‑100 (MQ=255)                                                                                          tAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACtt    >  2:2488227/1‑100 (MQ=255)                                                                                          tAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACtt    <  2:1345265/100‑1 (MQ=255)                                                                                          tAAGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACtt    <  2:162264/100‑1 (MQ=255)                                                                                           aaGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTa   >  1:925512/1‑100 (MQ=255)                                                                                           aaGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTa   <  2:1174047/100‑1 (MQ=255)                                                                                            aGATGGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTAc  <  2:810483/100‑1 (MQ=255)                                                                                                |                                                                                                ATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATAGCGGCTTAATGGCCGTCTTTTCAGGTGCTGCACCATCATTAAGAACTCCTGGTAGTCAAAGTTAAAGTGCGGCATTTACTGACGTTATAACTTAC  >  NC_000913/2000890‑2001080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑