Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,440,106 G→A S5S (AGC→AGT yfcJ ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,440,1060GA97.9% 307.6 / ‑2.4 94S5S (AGC→AGTyfcJputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (46/46);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

TAACCGGCAACGGCAACCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGG  >  NC_000913/2440009‑2440201
                                                                                                 |                                                                                               
tAACCGGCAACGGCAACCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAACTGGCAGAAGATCGTGTTTCGGTTTGACt                                                                                               <  1:2322372/100‑1 (MQ=255)
 aaCCGGCAACGGCAACCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACtt                                                                                              <  1:278576/100‑1 (MQ=255)
  aCCGGCAACGGCAACCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTa                                                                                             >  1:75481/1‑100 (MQ=255)
   ccGGCAACGGCAACCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTAc                                                                                            <  1:1733030/100‑1 (MQ=255)
   ccGGCAACGGCAACCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTAc                                                                                            <  2:1843945/100‑1 (MQ=255)
    cggcaacggcaaCCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTAca                                                                                           >  2:1298964/1‑100 (MQ=255)
     ggcaacggcaacCCCACGGACAAGTAGGTGAGAAAAACCGCAAAAGCGATGAAAAAAAAAAAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTAcag                                                                                          <  2:427531/100‑1 (MQ=255)
       caacggcaacCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTAcagca                                                                                        >  1:1454862/1‑100 (MQ=255)
         acggcaacACTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGt                                                                                      <  2:2335036/100‑1 (MQ=255)
          cggcaacCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTc                                                                                     <  2:2271311/100‑1 (MQ=255)
                cccTACGGTCATGGAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGAAGTCATGCAt                                                                               >  2:884414/1‑100 (MQ=255)
                  cTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGAGGCGGAAGGGCGAAAAATTGGCAGAAGATCGTGTTTCGGGTTGACTTACAGCAGTCATGCATTa                                                                             >  2:2466894/1‑100 (MQ=255)
                   tACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTAc                                                                            <  2:71837/100‑1 (MQ=255)
                      ggTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCGTGCATTACTcc                                                                         <  2:506290/100‑1 (MQ=255)
                      ggTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTcc                                                                         <  2:2179048/100‑1 (MQ=255)
                      ggTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGAAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTcc                                                                         <  1:1138735/100‑1 (MQ=255)
                       gTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCa                                                                        <  1:1249697/100‑1 (MQ=255)
                       gTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCa                                                                        <  1:414447/100‑1 (MQ=255)
                         cATGTAGGTGAGAAAAACCGCAAAACCGATGCGGAAGAGCGAAAAATGGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGa                                                                      >  1:2365087/1‑100 (MQ=255)
                           tgtgGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAAt                                                                    <  1:765126/100‑1 (MQ=255)
                           tGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAAt                                                                    >  2:2316680/1‑100 (MQ=255)
                           tGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAAt                                                                    >  2:2423519/1‑100 (MQ=255)
                           tGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAAt                                                                    >  1:10763/1‑100 (MQ=255)
                           tGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAAt                                                                    >  1:855785/1‑100 (MQ=255)
                            gTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTAAAGCAGTCATGAATTACTCCAGAATg                                                                   >  2:1225124/1‑100 (MQ=255)
                             tAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGc                                                                  <  2:875443/100‑1 (MQ=255)
                             tAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGc                                                                  >  1:580696/1‑100 (MQ=255)
                             tAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGc                                                                  >  1:903331/1‑100 (MQ=255)
                             tAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACCCCAGAATGc                                                                  >  2:800031/1‑100 (MQ=255)
                              aGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCa                                                                 <  2:2324762/100‑1 (MQ=255)
                                gTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAgc                                                               >  2:2049571/1‑100 (MQ=255)
                                gTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAgc                                                               >  1:1948041/1‑100 (MQ=255)
                                gTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAgc                                                               >  1:1414767/1‑100 (MQ=255)
                                 tGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAgcg                                                              >  1:38951/1‑100 (MQ=255)
                                   agaAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCa                                                            >  2:887487/1‑100 (MQ=255)
                                    gaAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGCAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCaa                                                           >  2:95911/1‑100 (MQ=255)
                                    gaAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCaa                                                           >  2:630664/1‑100 (MQ=255)
                                    gaAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCaa                                                           >  2:2062125/1‑100 (MQ=255)
                                    gaAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCaa                                                           >  1:1832985/1‑100 (MQ=255)
                                    gaAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCaa                                                           >  2:1635735/1‑100 (MQ=255)
                                    gaAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTGCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCaa                                                           >  2:1015693/1‑100 (MQ=255)
                                     aaaaaCCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAg                                                          <  2:1818720/100‑1 (MQ=255)
                                        aaCCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCg                                                       <  2:1085983/100‑1 (MQ=255)
                                           cGCAAAATTTATGCTGTAGAGCGAAAAATCGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCgagg                                                    >  2:645618/1‑100 (MQ=255)
                                           cGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCgagg                                                    >  1:1472829/1‑100 (MQ=255)
                                            gCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCgagga                                                   >  1:2194744/1‑100 (MQ=255)
                                            gCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCgagga                                                   <  2:592752/100‑1 (MQ=255)
                                             cAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCgaggag                                                  <  2:1489918/100‑1 (MQ=255)
                                              aaaaGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGt                                                 >  1:451875/1‑100 (MQ=255)
                                               aaaGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTa                                                >  2:238349/1‑100 (MQ=255)
                                                aaGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTAt                                               <  2:2511239/100‑1 (MQ=255)
                                                aaGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACGCCAGAATGCAGCGCAAGGCGAGGAGTAt                                               <  1:655023/100‑1 (MQ=255)
                                                  gCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATcc                                             <  1:2415326/100‑1 (MQ=255)
                                                   cGATGCGAGAGAGCGAAACATTGGCAGAAGATCGTGTTTCGGTTTGACTGACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATccc                                            <  2:682824/100‑1 (MQ=255)
                                                       gcagAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGtc                                        <  2:751939/97‑1 (MQ=255)
                                                       gCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGtc                                        <  1:1542194/100‑1 (MQ=255)
                                                       gCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGtc                                        <  2:1124187/100‑1 (MQ=255)
                                                         ggAAGGGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGtctc                                      >  2:1117337/1‑100 (MQ=255)
                                                         ggAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGtctc                                      <  2:987304/100‑1 (MQ=255)
                                                          gAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCa                                     >  1:2629074/1‑100 (MQ=255)
                                                           aaGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCAt                                    >  2:1890752/1‑100 (MQ=255)
                                                            agagCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCAtc                                   <  1:2586650/100‑1 (MQ=255)
                                                              agCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCAtctc                                 <  1:2599254/100‑1 (MQ=255)
                                                               gCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCAtctct                                <  1:1585324/100‑1 (MQ=255)
                                                               gCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCAtctct                                >  2:57491/1‑100 (MQ=255)
                                                               gCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAAGGCAGCGCAAGGCGAAGAGTATCCCCGTCTCAtctct                                >  2:2458544/1‑100 (MQ=255)
                                                                 gAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATctctct                              <  1:510866/100‑1 (MQ=255)
                                                                 gAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATctctct                              <  1:633739/100‑1 (MQ=255)
                                                                 gAAAAATTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATctctct                              >  1:2361401/1‑100 (MQ=255)
                                                                   aaaaTTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTgg                            <  1:2495798/100‑1 (MQ=255)
                                                                     aaTTGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGtt                          <  2:1741234/100‑1 (MQ=255)
                                                                       ttGGCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTc                        <  1:508892/100‑1 (MQ=255)
                                                                         ggCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAg                      <  1:362812/100‑1 (MQ=255)
                                                                          gCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAgg                     >  1:1758605/1‑100 (MQ=255)
                                                                          gCAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAgg                     >  2:309730/1‑100 (MQ=255)
                                                                           cAGAAGATCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAggg                    <  2:1434656/100‑1 (MQ=255)
                                                                            agaagaTCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGt                   >  1:242146/1‑100 (MQ=255)
                                                                             gaagaTCGTATTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTATCTGGTTTCAGGGtt                  >  2:180634/1‑100 (MQ=255)
                                                                             gaagaTCGGGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGGGTATCCCCGTCTCATCTCTCTGGTTTCAGGGtt                  >  2:180845/1‑100 (MQ=255)
                                                                              aagaTCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTa                 <  1:1402022/100‑1 (MQ=255)
                                                                               agaTCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTAc                <  2:1734915/100‑1 (MQ=255)
                                                                                 gtCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCCGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTAcag              >  1:2509108/2‑98 (MQ=255)
                                                                                 aTCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTAcag              >  1:1944330/1‑98 (MQ=255)
                                                                                  tCGTGTTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGt             >  1:413682/1‑100 (MQ=255)
                                                                                     tgtTTCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCg          >  2:2313287/1‑100 (MQ=255)
                                                                                        ttCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTg       >  1:421396/1‑100 (MQ=255)
                                                                                         tCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCTGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTgg      <  1:167631/100‑1 (MQ=255)
                                                                                         tCGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTgg      <  2:1361873/100‑1 (MQ=255)
                                                                                          cGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGc     <  2:1370342/100‑1 (MQ=255)
                                                                                          cGGTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGc     <  2:1371558/100‑1 (MQ=255)
                                                                                           ggTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCa    >  1:963386/1‑100 (MQ=255)
                                                                                           ggTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCa    <  2:2237444/100‑1 (MQ=255)
                                                                                            gTTTGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAg   <  2:633927/100‑1 (MQ=255)
                                                                                             tttGACTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAgg  >  2:1393452/1‑100 (MQ=255)
                                                                                                 |                                                                                               
TAACCGGCAACGGCAACCCTACGGTCATGTAGGTGAGAAAAACCGCAAAAGCGATGCGGAAGAGCGAAAAATTGGCAGAAGATCGTGTTTCGGTTTGGCTTACAGCAGTCATGCATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGG  >  NC_000913/2440009‑2440201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: