BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	171,072	A→G	K166K (AAA→AAG)	fhuD →	iron(3+)‑hydroxamate import ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	171,072	0	A	G	100.0%	424.2 / NA	122	K166K (AAA→AAG)	fhuD	iron(3+)‑hydroxamate import ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (58/64); total (58/64)

Only 100 of 122 total aligned reads displayed.

CGCGTAAATCGCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAACCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCA  >  NC_000913/170975‑171167

cgcgTAAATCGCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCCATATGAAGACTTTATCCGCAGCATGAAGcc                                                                                               >  1:1042661/1‑100 (MQ=255)

cgcgTAAATCGCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGcc                                                                                               >  1:2282558/1‑100 (MQ=255)

 gcgTAAATCGCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGccc                                                                                              <  2:1113644/100‑1 (MQ=255)

  cgTAAATCGCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGcccc                                                                                             >  1:1245126/1‑100 (MQ=255)

      aaTCGCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCtt                                                                                         <  1:1000224/100‑1 (MQ=255)

         cGCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTtgt                                                                                      <  1:2071901/100‑1 (MQ=255)

         cGCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTtgt                                                                                      <  1:2385063/100‑1 (MQ=255)

          gCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTtgtg                                                                                     <  2:723553/100‑1 (MQ=255)

           cTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGa                                                                                    <  1:2498871/100‑1 (MQ=255)

              aCGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGc                                                                                 <  1:1148750/100‑1 (MQ=255)

                 gAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCgtg                                                                              >  2:1939214/1‑100 (MQ=255)

                  aaaTGGCAGATTTACTTAACCTGCAAAGCGCAGCGGACACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCgtgg                                                                             <  1:2460874/100‑1 (MQ=255)

                  aaaTGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCgtgg                                                                             <  1:1404174/100‑1 (MQ=255)

                  aaaTGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCgtgg                                                                             <  2:1674042/100‑1 (MQ=255)

                    aTGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGACGCgtggtg                                                                           >  2:100139/1‑100 (MQ=255)

                      ggCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTgcg                                                                         <  1:2308874/100‑1 (MQ=255)

                      ggCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTgcg                                                                         >  2:1658609/1‑100 (MQ=255)

                      ggCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTgcg                                                                         <  1:1534475/100‑1 (MQ=255)

                        cAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGcgcg                                                                       >  2:11600/1‑100 (MQ=255)

                        cAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGcgcg                                                                       <  1:1345497/100‑1 (MQ=255)

                        cAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGcgcg                                                                       >  1:1252717/1‑100 (MQ=255)

                         aGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGt                                                                      <  1:565685/100‑1 (MQ=255)

                          gATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTc                                                                     <  2:844427/100‑1 (MQ=255)

                           aTTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTcc                                                                    >  1:1367183/1‑100 (MQ=255)

                            tttACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCg                                                                   <  2:1809919/100‑1 (MQ=255)

                              tACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGtt                                                                 >  2:385481/1‑100 (MQ=255)

                               aCTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTa                                                                <  1:61524/100‑1 (MQ=255)

                               aCTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTa                                                                <  1:1164420/100‑1 (MQ=255)

                                 ttAACCTGCAAAGCGAAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTAtt                                                              <  2:1585768/100‑1 (MQ=255)

                                  tAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTg                                                             >  1:2274023/1‑100 (MQ=255)

                                   aaCCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGc                                                            <  1:1838073/100‑1 (MQ=255)

                                   aaCCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGc                                                            <  2:444201/100‑1 (MQ=255)

                                      cTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTga                                                         >  2:331152/1‑100 (MQ=255)

                                       tGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTgac                                                        >  1:1902996/1‑100 (MQ=255)

                                       tGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTgac                                                        >  1:962249/1‑100 (MQ=255)

                                       tGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTgac                                                        >  1:1304477/1‑100 (MQ=255)

                                       tGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGCCCGTTATTGCTgac                                                        >  1:2220531/1‑100 (MQ=255)

                                        gCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTgacg                                                       >  2:556257/1‑100 (MQ=255)

                                          aacgcgcAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTgacgac                                                     >  1:1578541/4‑100 (MQ=255)

                                            aGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGc                                                   <  2:2285849/100‑1 (MQ=255)

                                            aGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGc                                                   <  2:256217/100‑1 (MQ=255)

                                             gcgcAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCt                                                  >  1:1665373/1‑100 (MQ=255)

                                              cgcAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCtt                                                 >  1:1055914/1‑100 (MQ=255)

                                               gcAGCGGAAACGCATGTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTa                                                <  2:753842/100‑1 (MQ=255)

                                                cAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTAt                                               >  1:1757981/1‑100 (MQ=255)

                                                 aGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATc                                              <  1:14968/100‑1 (MQ=255)

                                                 aGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATc                                              <  2:421325/100‑1 (MQ=255)

                                                 aGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATc                                              <  2:1409114/100‑1 (MQ=255)

                                                   cGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGa                                            <  1:221369/100‑1 (MQ=255)

                                                    ggAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGAt                                           >  1:785963/1‑100 (MQ=255)

                                                    ggAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGAt                                           >  2:75244/1‑100 (MQ=255)

                                                    ggAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGAt                                           >  2:75261/1‑100 (MQ=255)

                                                      aaaCGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATcc                                         <  1:1036926/100‑1 (MQ=255)

                                                       aaCGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCcg                                        <  1:2223119/100‑1 (MQ=255)

                                                         cGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCcgcg                                      >  1:1868/1‑100 (MQ=255)

                                                         cGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCcgcg                                      >  2:466819/1‑100 (MQ=255)

                                                         cGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCACGTCCGGTATTGCTGACGAAGCTTATCGCTCcgcg                                      >  2:1210071/1‑100 (MQ=255)

                                                           cATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGcc                                    >  1:1002447/1‑100 (MQ=255)

                                                           cATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGGGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGcc                                    <  2:1757617/100‑1 (MQ=255)

                                                             tttAGCGCAATATGCAGACATTATAAGCAGCATGAAGCCACGCTTACTAAAGCGTGGTGCGCGTCCGTCATCGATGTCGATGCATATCGTTCAGCGCCac                                  >  2:1702710/1‑99 (MQ=255)

                                                             tttAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCat                                  <  1:507771/100‑1 (MQ=255)

                                                              ttAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCata                                 <  2:1556893/100‑1 (MQ=255)

                                                               tAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCatat                                >  1:1778104/1‑100 (MQ=255)

                                                               tAGCGCAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCatat                                >  1:326338/1‑100 (MQ=255)

                                                                 gcgcAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGc                              <  1:2192328/100‑1 (MQ=255)

                                                                 gcgcAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGATGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGc                              >  2:498646/1‑100 (MQ=255)

                                                                  cgcAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCt                             <  2:525379/100‑1 (MQ=255)

                                                                  cgcAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCt                             >  1:1205940/1‑100 (MQ=255)

                                                                  cgcAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATACGCt                             >  1:1216792/1‑100 (MQ=255)

                                                                    cAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTgg                           <  1:76359/100‑1 (MQ=255)

                                                                    cAATATGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTgg                           <  1:76376/100‑1 (MQ=255)

                                                                      atatGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTc                         <  1:1410808/100‑1 (MQ=255)

                                                                       tatGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCt                        >  2:1900807/1‑100 (MQ=255)

                                                                        atGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCtt                       >  1:1634216/1‑100 (MQ=255)

                                                                        atGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCtt                       >  2:631704/1‑100 (MQ=255)

                                                                         tGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTc                      >  2:1589874/1‑100 (MQ=255)

                                                                         tGAAGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTc                      >  2:1948828/1‑100 (MQ=255)

                                                                           aaGACTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCgg                    <  2:911015/100‑1 (MQ=255)

                                                                              aCTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTcc                 <  2:1249925/100‑1 (MQ=255)

                                                                               cTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCa                >  2:785396/1‑100 (MQ=255)

                                                                               cTTTATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCa                >  2:2235670/1‑100 (MQ=255)

                                                                                 ttATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCaaa              <  2:1217608/100‑1 (MQ=255)

                                                                                 ttATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCaaa              >  1:183193/1‑100 (MQ=255)

                                                                                 ttATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCaaa              <  2:1217313/100‑1 (MQ=255)

                                                                                  tATCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAAc             <  1:882064/100‑1 (MQ=255)

                                                                                    tCCGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAg           >  2:621841/1‑100 (MQ=255)

                                                                                     ccGCAGCATGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGc          >  2:1911000/1‑100 (MQ=255)

                                                                                       gcagcaTGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCtt        >  2:161380/1‑100 (MQ=255)

                                                                                       gcagcaTGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCtt        >  1:64922/1‑100 (MQ=255)

                                                                                       gcagcaTGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCtt        <  1:476451/100‑1 (MQ=255)

                                                                                        cagcaTGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTg       <  2:1285795/100‑1 (MQ=255)

                                                                                        cagcaTGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTg       <  1:750188/100‑1 (MQ=255)

                                                                                            aagAAGCCCCGCTTTGTAAAGCGTGGTACGCGTCTGTTATTGCTGACGACGCTTATCTATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTcc   <  2:1409251/98‑1 (MQ=255)

                                                                                            aTGAAGCCCCGCTTTGTGCACCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTcc   <  1:2477825/100‑1 (MQ=255)

                                                                                            aTGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTcc   <  2:417187/100‑1 (MQ=255)

                                                                                            aTGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTcc   >  2:134755/1‑100 (MQ=255)

                                                                                             tGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCa  >  2:363436/1‑100 (MQ=255)

                                                                                             tGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCa  >  2:1663029/1‑100 (MQ=255)

                                                                                             tGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCa  <  1:543733/100‑1 (MQ=255)

                                                                                             tGAAGCCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCa  >  2:2095517/1‑100 (MQ=255)

CGCGTAAATCGCTGACGGAAATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGACTTTATCCGCAGCATGAAACCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACGACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCA  >  NC_000913/170975‑171167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`