Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,174,146 C→T V417V (GTG→GTA parE ← DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,174,1460CT98.4% 422.1 / ‑2.5 126V417V (GTG→GTAparEDNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (66/56);  total (68/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 129 total aligned reads displayed.

CTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGCACC  >  NC_000913/3174049‑3174239
                                                                                                 |                                                                                             
 ttCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGTGGAGTCACCTTCtaca                                                                                            <  2:2517318/100‑1 (MQ=255)
  tCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACaa                                                                                           >  1:2565881/1‑100 (MQ=255)
   cccAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAg                                                                                          >  1:625580/1‑100 (MQ=255)
   cccAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAg                                                                                          <  1:1483567/100‑1 (MQ=255)
    ccAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAgg                                                                                         <  2:1065253/100‑1 (MQ=255)
     cAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGa                                                                                        <  2:62417/100‑1 (MQ=255)
     cAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGa                                                                                        <  2:1368619/100‑1 (MQ=255)
       gggtttAATGCTCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGCTCGCCCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACACGGAAc                                                                                      <  2:306625/96‑1 (MQ=255)
       ggTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAAGAAc                                                                                      <  2:1766220/100‑1 (MQ=255)
        gtgtTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACACGGcaca                                                                                     >  2:1187788/1‑100 (MQ=255)
        gtgtTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACa                                                                                     >  2:914427/1‑100 (MQ=255)
         tgtTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAg                                                                                    >  1:158635/1‑100 (MQ=255)
            tAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTc                                                                                 >  1:1622699/1‑100 (MQ=255)
             aaGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCg                                                                                >  2:29667/1‑100 (MQ=255)
             aaGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCg                                                                                >  1:1834913/1‑100 (MQ=255)
               ggATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGt                                                                              >  1:1289858/1‑100 (MQ=255)
                 aTCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTAc                                                                            <  1:1603631/100‑1 (MQ=255)
                   cTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACgg                                                                          >  1:844727/1‑100 (MQ=255)
                    ttACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGt                                                                         <  2:742318/100‑1 (MQ=255)
                     tACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGtt                                                                        >  2:160260/1‑100 (MQ=255)
                     tACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATACGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGtt                                                                        <  2:1706419/100‑1 (MQ=255)
                       ccTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTaa                                                                      >  1:2634500/1‑100 (MQ=255)
                       ccTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTaa                                                                      >  1:4201/1‑100 (MQ=255)
                       ccTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTaa                                                                      >  1:1195978/1‑100 (MQ=255)
                       ccTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTaa                                                                      >  1:2380200/1‑100 (MQ=255)
                         tttCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAgg                                                                    >  2:1944499/1‑100 (MQ=255)
                         tttCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAgg                                                                    >  1:207665/1‑100 (MQ=255)
                         tttCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAgg                                                                    <  2:2224075/100‑1 (MQ=255)
                         tttCAGTGGCATGATCGCCTGATATTCGCGATCCCTCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGGACAGCTCGGTCCGGTTAAgg                                                                    >  2:1649050/1‑100 (MQ=255)
                           tCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTc                                                                  <  2:754936/100‑1 (MQ=255)
                            cAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTcc                                                                 <  2:1174142/100‑1 (MQ=255)
                             aGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCt                                                                <  1:693523/100‑1 (MQ=255)
                              gTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTg                                                               >  2:2565006/1‑100 (MQ=255)
                                ggCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGGCTGGGGAGTCACCTTCGACAAGGAACAGCTCGGTACGGCTAGGGTCCTgcg                                                             >  1:1727930/1‑100 (MQ=255)
                                ggCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTgcg                                                             <  2:1686091/100‑1 (MQ=255)
                                 gCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTgcgc                                                            <  1:847079/100‑1 (MQ=255)
                                 gCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTgcgc                                                            >  1:656011/1‑100 (MQ=255)
                                 gCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTgcgc                                                            <  1:165348/100‑1 (MQ=255)
                                  cATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGcgcg                                                           >  1:1402766/1‑100 (MQ=255)
                                   aTGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCgg                                                          <  2:958135/100‑1 (MQ=255)
                                   aTGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCgg                                                          <  1:1540455/100‑1 (MQ=255)
                                    tGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGt                                                         <  1:1032673/100‑1 (MQ=255)
                                    tGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGt                                                         <  1:131725/100‑1 (MQ=255)
                                    tGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGt                                                         <  1:155880/100‑1 (MQ=255)
                                    tGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGt                                                         <  1:1791903/100‑1 (MQ=255)
                                    tGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGt                                                         <  1:1983921/100‑1 (MQ=255)
                                    tGATCGCCTGATATTCGCGACCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGt                                                         >  1:2060074/1‑100 (MQ=255)
                                     gATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACCAGGACCCGCTCGGTACGGTTAAGGTCCTGCGCGGTa                                                        >  1:373659/1‑100 (MQ=255)
                                     gATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTa                                                        <  2:1569447/100‑1 (MQ=255)
                                      aTCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTAc                                                       <  2:1676477/100‑1 (MQ=255)
                                          ccTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATc                                                   <  1:1484916/100‑1 (MQ=255)
                                            tGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAg                                                 <  1:2579436/100‑1 (MQ=255)
                                            tGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAg                                                 >  1:2154595/1‑100 (MQ=255)
                                            tGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAg                                                 >  1:442869/1‑100 (MQ=255)
                                              atatTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGcc                                               <  1:933744/100‑1 (MQ=255)
                                               tatTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCa                                              >  1:2261204/1‑100 (MQ=255)
                                               tatTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCa                                              >  1:2323810/1‑100 (MQ=255)
                                                atTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAg                                             >  2:2574803/1‑100 (MQ=255)
                                                atTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAg                                             >  1:1489775/1‑100 (MQ=255)
                                                 ttCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGt                                            >  1:1047250/1‑100 (MQ=255)
                                                  tCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGtt                                           <  1:2268726/100‑1 (MQ=255)
                                                  tCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGtt                                           >  1:2231599/1‑100 (MQ=255)
                                                  tCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGtt                                           >  2:2582/1‑100 (MQ=255)
                                                     cgATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGc                                        >  2:1939813/1‑100 (MQ=255)
                                                     cgATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGc                                        >  1:776608/1‑100 (MQ=255)
                                                      gATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGcc                                       >  2:2430371/1‑100 (MQ=255)
                                                        tCGCGTGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAg                                     <  2:733445/100‑1 (MQ=255)
                                                           cgcgcCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCa                                  <  2:1754109/100‑1 (MQ=255)
                                                                cTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGcc                             <  1:35170/100‑1 (MQ=255)
                                                                   cTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCggg                          >  1:790109/1‑100 (MQ=255)
                                                                   cTTGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCggg                          <  2:1040481/100‑1 (MQ=255)
                                                                     tGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGcc                        <  2:1885365/100‑1 (MQ=255)
                                                                     tGGCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGcc                        <  1:849355/100‑1 (MQ=255)
                                                                      ggCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCg                       >  1:1734416/1‑100 (MQ=255)
                                                                       gCAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGc                      >  2:1863488/1‑100 (MQ=255)
                                                                        cAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCt                     <  1:1770463/100‑1 (MQ=255)
                                                                        cAGATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCt                     >  2:1359066/1‑100 (MQ=255)
                                                                          gATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTgg                   <  1:547757/100‑1 (MQ=255)
                                                                          gATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTgg                   >  2:517763/1‑100 (MQ=255)
                                                                          gATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTgg                   >  2:1594361/1‑100 (MQ=255)
                                                                          gATCCGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTgg                   <  1:108102/100‑1 (MQ=255)
                                                                            tCCGCCTGCGGAGTCACCTTCAACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGTTACAATCAGCCAGTTTGCCAGGCACCGCCGGGCCGCTGGTc                 <  2:1216006/100‑1 (MQ=255)
                                                                              cGCCTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAg               >  2:378238/1‑100 (MQ=255)
                                                                                 cTGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCtt            >  1:1859030/1‑100 (MQ=255)
                                                                                  tGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCttt           >  1:948083/1‑100 (MQ=255)
                                                                                  tGCGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCttt           >  1:284135/1‑100 (MQ=255)
                                                                                    cGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCCGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCttttt         >  2:1746982/1‑100 (MQ=255)
                                                                                    cGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCttttt         <  1:1724834/100‑1 (MQ=255)
                                                                                    cGGAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCttttt         <  1:157874/100‑1 (MQ=255)
                                                                                    cGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCttttt         <  2:1398719/100‑1 (MQ=255)
                                                                                      gAGTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTAc       <  1:2488906/100‑1 (MQ=255)
                                                                                        gTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGc     >  1:2453996/1‑100 (MQ=255)
                                                                                        gTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGc     <  2:2266972/100‑1 (MQ=255)
                                                                                        gTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGc     <  1:336844/100‑1 (MQ=255)
                                                                                        gTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGc     >  1:845975/1‑100 (MQ=255)
                                                                                        gTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGc     <  1:162651/100‑1 (MQ=255)
                                                                                        gTCACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGATACAATCAGCCAGTTAGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGc     >  2:578299/1‑100 (MQ=255)
                                                                                          cACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGcac   <  1:584761/100‑1 (MQ=255)
                                                                                          cACCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGcac   >  2:908312/1‑100 (MQ=255)
                                                                                           aCCTTCTACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGcacc  >  2:302997/1‑100 (MQ=255)
                                                                                                 |                                                                                             
CTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGCACC  >  NC_000913/3174049‑3174239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: