Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,183,258 G→A E672K (GAA→AAA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,183,2580GA96.9% 425.1 / 1.6 129E672K (GAA→AAA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (59/66);  total (60/69)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 129 total aligned reads displayed.

GGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTA  >  NC_000913/4183162‑4183354
                                                                                                |                                                                                                
ggCGAATCCAGCTTATTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAac                                                                                               <  2:513572/100‑1 (MQ=255)
  cGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAacac                                                                                             >  2:1434962/1‑100 (MQ=255)
    aaTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGa                                                                                           <  1:514170/100‑1 (MQ=255)
     aTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGAt                                                                                          <  2:1116973/100‑1 (MQ=255)
     aTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGAt                                                                                          >  2:358920/1‑100 (MQ=255)
        cAGCTTGTTCCGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATACCGTTCCTGAAACACGATGAc                                                                                       >  2:2455779/1‑100 (MQ=255)
        cAGCTTGTTCAGCCGCGACCAGGTTGACTAGATGAACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGAc                                                                                       <  2:2147088/100‑1 (MQ=255)
          gCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGc                                                                                     <  2:2325772/100‑1 (MQ=255)
          gCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGc                                                                                     <  2:1864103/100‑1 (MQ=255)
           cTTGTTCAGCCGCGACCAGGTTGACTACAGGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGcc                                                                                    <  2:1184239/100‑1 (MQ=255)
            ttGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCa                                                                                   <  1:694526/100‑1 (MQ=255)
               ttCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAAcc                                                                                >  2:690922/1‑100 (MQ=255)
               ttCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAAcc                                                                                <  2:2085641/100‑1 (MQ=255)
               tacAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCACGATCCTGAAACACGATGACGCCAAcc                                                                                >  2:197891/3‑100 (MQ=255)
                 cAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGt                                                                              >  2:1997864/1‑100 (MQ=255)
                   gCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGc                                                                            >  2:1348652/1‑100 (MQ=255)
                   gCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGc                                                                            <  2:232525/100‑1 (MQ=255)
                   gCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGc                                                                            >  2:1348672/1‑100 (MQ=255)
                   gCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGCATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGc                                                                            >  1:579099/1‑100 (MQ=255)
                    ccGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCa                                                                           <  1:2266848/100‑1 (MQ=255)
                    ccGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCa                                                                           <  1:1941970/100‑1 (MQ=255)
                      gcgACCAGGTTGACTACATTGACGTATCCACCCAGCACGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACATGATGACGCCAACCGTGCAtt                                                                         <  2:551664/100‑1 (MQ=255)
                          ccAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATg                                                                     <  1:2226080/100‑1 (MQ=255)
                          ccAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATg                                                                     <  2:2071524/100‑1 (MQ=255)
                           cAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATgg                                                                    >  1:85081/1‑100 (MQ=255)
                            aGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATggg                                                                   >  1:977552/1‑100 (MQ=255)
                             ggTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGt                                                                  <  2:690063/100‑1 (MQ=255)
                             ggTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGt                                                                  >  2:1839524/1‑100 (MQ=255)
                             ggTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGt                                                                  >  2:694785/1‑100 (MQ=255)
                             ggTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGt                                                                  >  1:1043667/1‑100 (MQ=255)
                              ggtGACTACATGGACGTACCCACCCAGCAGGTGGTCTCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTg                                                                 <  1:2530306/98‑1 (MQ=255)
                              gTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTg                                                                 <  2:1079739/100‑1 (MQ=255)
                              gTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTg                                                                 >  1:2553750/1‑100 (MQ=255)
                                tGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCg                                                               >  1:1648573/1‑100 (MQ=255)
                                tGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCg                                                               <  1:1434414/100‑1 (MQ=255)
                                 gACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGa                                                              <  1:2510018/100‑1 (MQ=255)
                                 gACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGa                                                              <  2:67803/100‑1 (MQ=255)
                                  aCTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGaa                                                             <  1:748977/100‑1 (MQ=255)
                                   cTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAAc                                                            >  2:560264/1‑100 (MQ=255)
                                      cATGGACGTATGCACCCCGCAAGTGGCATCCGTCGCTGCGTCCCTGACCCCGTTCCTGAAACACGATGTCGCCAACCGTGCATTGATGGGAGTGAACATg                                                         >  2:1547935/1‑100 (MQ=255)
                                      cATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                         <  1:1690456/100‑1 (MQ=255)
                                      cATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                         >  1:419528/1‑100 (MQ=255)
                                      cATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                         <  1:1439253/100‑1 (MQ=255)
                                      cATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTACATTGATGGGTGCGAACATg                                                         >  2:1560917/1‑100 (MQ=255)
                                       aTGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGc                                                        >  1:1041887/1‑100 (MQ=255)
                                       aTGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGc                                                        <  2:1025607/100‑1 (MQ=255)
                                        tGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCa                                                       <  2:750578/100‑1 (MQ=255)
                                        tGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAAACGTGCAGTGATGGGTGCGAACATGCa                                                       >  2:2125248/1‑100 (MQ=255)
                                          gACGTATCCACCCAGCAGGTGGTATCCGGCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGGGCGAACATGCAAc                                                     >  2:880932/1‑100 (MQ=255)
                                              tATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCa                                                 >  2:967394/1‑100 (MQ=255)
                                              tATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCa                                                 >  2:213623/1‑100 (MQ=255)
                                                tCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                               >  2:1202059/1‑100 (MQ=255)
                                                 ccccccAGCAGGTGGTATGCCTCGGTTCGTCCCTGATCCTGTTCCCGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                              <  1:2472003/97‑1 (MQ=255)
                                                 ccACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                              <  2:1789314/100‑1 (MQ=255)
                                                  cACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGcc                                             <  2:1998990/100‑1 (MQ=255)
                                                  cACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGcc                                             >  1:390140/1‑100 (MQ=255)
                                                     ccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGtt                                          >  1:328675/1‑100 (MQ=255)
                                                       agcagGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTcc                                        <  1:2179611/100‑1 (MQ=255)
                                                       agcagGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTcc                                        <  1:2154496/100‑1 (MQ=255)
                                                        gcagGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCg                                       >  2:1652456/1‑100 (MQ=255)
                                                         cagGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGa                                      >  1:79308/1‑100 (MQ=255)
                                                           ggtggtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGTGTGCGAACATGCAACGGCAGGCCGTTCCGAct                                    >  1:1446659/1‑100 (MQ=255)
                                                           ggtggtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                    >  1:1436319/1‑100 (MQ=255)
                                                           ggtggtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                    >  2:1954221/1‑100 (MQ=255)
                                                              ggtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTg                                 >  2:212138/1‑100 (MQ=255)
                                                              ggtATCCGTCAGTGCGACCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTg                                 >  1:2313255/1‑100 (MQ=255)
                                                               gtATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgc                                <  2:1146353/100‑1 (MQ=255)
                                                                tATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg                               >  1:2294933/1‑100 (MQ=255)
                                                                tATCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg                               >  1:80626/1‑100 (MQ=255)
                                                                 aTCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgc                              >  1:2216162/1‑100 (MQ=255)
                                                                  tCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcg                             <  1:1249742/100‑1 (MQ=255)
                                                                  tCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcg                             <  1:939129/100‑1 (MQ=255)
                                                                  tCCGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcg                             <  2:1155579/100‑1 (MQ=255)
                                                                    cGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCCACGTCAGGCCGTTCCGACTCTGCGCGCt                           >  2:1241621/1‑100 (MQ=255)
                                                                    cGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCt                           <  1:293276/100‑1 (MQ=255)
                                                                    cGTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACAGGCAACGTCAGGCCGTTCCGACTCTGCGCGCt                           <  1:2120801/100‑1 (MQ=255)
                                                                     gTCGGTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTg                          <  1:676811/100‑1 (MQ=255)
                                                                        ggTGCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATa                       <  1:1625213/100‑1 (MQ=255)
                                                                           gCGTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGc                    <  1:1950927/100‑1 (MQ=255)
                                                                            cGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGcc                   <  1:1262134/100‑1 (MQ=255)
                                                                             gTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                  >  2:2300496/1‑100 (MQ=255)
                                                                             gTCCCTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                  <  1:778845/100‑1 (MQ=255)
                                                                               cccTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCt                >  1:1161389/1‑100 (MQ=255)
                                                                               cccTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCt                >  2:37548/1‑100 (MQ=255)
                                                                                 cTGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtgg              <  1:1134452/100‑1 (MQ=255)
                                                                                  tGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt             <  2:2028951/100‑1 (MQ=255)
                                                                                  tGATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt             <  1:351423/100‑1 (MQ=255)
                                                                                   gATCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggtt            <  1:2271080/100‑1 (MQ=255)
                                                                                    aTCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttg           <  2:1273775/100‑1 (MQ=255)
                                                                                    aTCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttg           >  1:195452/1‑100 (MQ=255)
                                                                                     tCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg          <  1:654295/100‑1 (MQ=255)
                                                                                     tCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg          <  2:743510/100‑1 (MQ=255)
                                                                                     tCCCGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg          <  2:788910/100‑1 (MQ=255)
                                                                                       ccGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGTGCTGATAAGCCGCTGGTTGGTa        >  2:707187/1‑100 (MQ=255)
                                                                                       ccGTGCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa        <  1:1986293/100‑1 (MQ=255)
                                                                                        cGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc       >  2:1264907/1‑100 (MQ=255)
                                                                                        cGTTCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc       <  1:2357676/100‑1 (MQ=255)
                                                                                           tCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTgg    <  1:1360084/100‑1 (MQ=255)
                                                                                           tCCTGAAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTgg    <  2:1487077/100‑1 (MQ=255)
                                                                                             cTGAAACACGATGCCGCCAACCGTGCATTCATGGGTGCGAACATGCAACGCCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACGGGTa  >  2:37764/1‑100 (MQ=255)
                                                                                                |                                                                                                
GGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTA  >  NC_000913/4183162‑4183354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: