Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,438,767 C→T A166T (GCG→ACG)  ytfJ ← putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,438,7670CT99.1% 355.8 / ‑4.5 110A166T (GCG→ACG) ytfJputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (61/48);  total (62/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

Only 100 of 113 total aligned reads displayed.

AGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGCCA  >  NC_000913/4438670‑4438861
                                                                                                 |                                                                                              
aGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGtcc                                                                                              <  2:1384921/100‑1 (MQ=255)
  gggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCGCCTCTTCCGGTGTGAGCGTCCCg                                                                                            <  2:783205/100‑1 (MQ=255)
  gggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCg                                                                                            >  1:2118748/1‑100 (MQ=255)
  gggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCg                                                                                            <  1:2564406/100‑1 (MQ=255)
  gggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCg                                                                                            <  1:1252733/100‑1 (MQ=255)
  agcaaaaTTATTTTTGCGAAATTTATGTTGTTGCCATTATTTTAGCAACTTTTGGAGCAGGTCCATCACTTTCTGCCTCTCTTTCGGTGTGAGCGTCCCg                                                                                            <  2:74814/97‑1 (MQ=255)
   ggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGt                                                                                           <  2:862095/100‑1 (MQ=255)
      aaaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGTTCCTCTTCCGGTGTTAGCGTCCCGTCtt                                                                                        <  1:2326254/100‑1 (MQ=255)
        attattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTg                                                                                      <  1:637941/100‑1 (MQ=255)
         ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCTACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTgg                                                                                     <  2:2170630/100‑1 (MQ=255)
          tattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGc                                                                                    <  2:1802041/100‑1 (MQ=255)
          tattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTGTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGc                                                                                    <  1:1444860/100‑1 (MQ=255)
               ttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCAtt                                                                               >  1:1743405/1‑100 (MQ=255)
               ttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCAtt                                                                               <  1:1491091/100‑1 (MQ=255)
               ttGCAGAATTTATGTTATTGCCATTATTTAAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCAtt                                                                               >  2:1169714/1‑100 (MQ=255)
                 gCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGc                                                                             >  1:744555/1‑100 (MQ=255)
                 gCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGc                                                                             >  2:2257971/1‑100 (MQ=255)
                 gCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGc                                                                             >  2:2258287/1‑100 (MQ=255)
                 gCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGc                                                                             >  1:1598565/1‑100 (MQ=255)
                  cAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCa                                                                            >  2:683944/1‑100 (MQ=255)
                  cAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCCGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCa                                                                            >  1:1044326/1‑100 (MQ=255)
                    gAATTTATGTTATTGCCATTATTTGAGCACCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCAcg                                                                          <  2:1458427/100‑1 (MQ=255)
                    gAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCAcg                                                                          >  2:1350639/1‑100 (MQ=255)
                      aTTTATGTTATTGCCATTATTTGAGCAACTTTTTCAGCAGGTCCATCACTTGCTCCACCTCTTCCGGTGTGAGCGTCCCTTCTTTGGCCCATTGCAcgcg                                                                        <  2:1542390/100‑1 (MQ=255)
                      aTTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCAcgcg                                                                        <  2:2538505/100‑1 (MQ=255)
                        ttATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGAc                                                                      <  2:138755/100‑1 (MQ=255)
                         tATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGAcc                                                                     >  1:276657/1‑100 (MQ=255)
                           tggtATTGCCATTATTTGCGCATCTTTTGCTGCTGGTCCATCACTGGCTGCACCCCTTCCGGGGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCAt                                                                   <  2:529839/97‑1 (MQ=255)
                            gTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATc                                                                  <  1:1082259/100‑1 (MQ=255)
                             ttATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATcc                                                                 >  1:1097738/1‑100 (MQ=255)
                              tATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCt                                                                <  1:308268/100‑1 (MQ=255)
                              tATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCt                                                                <  2:2325432/100‑1 (MQ=255)
                              tATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCt                                                                >  1:1557077/1‑100 (MQ=255)
                               aTTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCtt                                                               <  2:1509559/100‑1 (MQ=255)
                                 tGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTAt                                                             >  2:481253/1‑100 (MQ=255)
                                 tGCCATTATTTGAGCAACTTGTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTAt                                                             <  2:1188996/100‑1 (MQ=255)
                                  gCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATc                                                            >  1:40671/1‑100 (MQ=255)
                                  gCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATc                                                            >  1:40651/1‑100 (MQ=255)
                                  gCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATc                                                            >  2:363731/1‑100 (MQ=255)
                                  gCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATc                                                            <  2:1097954/100‑1 (MQ=255)
                                   ccATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATcc                                                           >  1:1706329/1‑100 (MQ=255)
                                   ccATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATcc                                                           >  1:233210/1‑100 (MQ=255)
                                       tattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGca                                                       <  1:408964/100‑1 (MQ=255)
                                         tttGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGcacc                                                     <  2:236596/100‑1 (MQ=255)
                                           tGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGcaccac                                                   <  1:1687397/100‑1 (MQ=255)
                                           tGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGcaccac                                                   >  1:2590007/1‑100 (MQ=255)
                                           tGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTGGGCCCATTGCACGCGACCATCCTTATCCAGcaccac                                                   >  1:2489020/1‑100 (MQ=255)
                                             aGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCCACCATCCTTATCCAGCACCACTa                                                 >  2:824154/1‑100 (MQ=255)
                                              gCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTAc                                                >  2:615402/1‑100 (MQ=255)
                                              gCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTAc                                                >  2:1037666/1‑100 (MQ=255)
                                              gCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTAc                                                <  1:966701/100‑1 (MQ=255)
                                              gCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTAc                                                >  2:1892708/1‑100 (MQ=255)
                                              gCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTGGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTAc                                                >  2:321120/1‑100 (MQ=255)
                                               cAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACa                                               >  1:690646/1‑100 (MQ=255)
                                                aaCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGTGACCATCCTTATCCAGCACCACTACAg                                              <  1:2557427/100‑1 (MQ=255)
                                                 aCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGc                                             >  1:1242975/1‑100 (MQ=255)
                                                 aCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGc                                             <  1:159918/100‑1 (MQ=255)
                                                 aCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGc                                             <  2:1485405/100‑1 (MQ=255)
                                                 aCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGc                                             >  2:293484/1‑100 (MQ=255)
                                                 aCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCGTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGc                                             <  2:1485406/100‑1 (MQ=255)
                                                  cTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCg                                            >  1:1374980/1‑100 (MQ=255)
                                                   ttttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCgg                                           <  2:844079/100‑1 (MQ=255)
                                                   ttttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCgg                                           >  1:1047727/1‑100 (MQ=255)
                                                       gcagcaGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACt                                       >  1:148834/1‑100 (MQ=255)
                                                       gcagcaGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACt                                       >  2:1095270/1‑100 (MQ=255)
                                                       gcagcaGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACt                                       <  2:1524664/100‑1 (MQ=255)
                                                        cagcagGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACtt                                      >  1:522686/1‑100 (MQ=255)
                                                         agaagGTCCATCACTAGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCCTTGGCCCCCTGCACGCGACCACCCTTATCCCGCGCCACTACAGCGGAAGttt                                     >  2:1756022/4‑100 (MQ=255)
                                                             ggTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTttcttc                                 <  1:127429/100‑1 (MQ=255)
                                                                 cATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATcc                             >  1:1041133/1‑100 (MQ=255)
                                                                     aattGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTCCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCt                         >  2:815262/3‑100 (MQ=255)
                                                                     aCTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCt                         >  1:1067589/1‑100 (MQ=255)
                                                                      cTTGCTGCACCTCTTCCGGTGTGAGCCTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTg                        <  1:1006120/100‑1 (MQ=255)
                                                                        tgctgcACCTCTTCCTGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCATCACCACTACAGCGGAACTTTCTTCATCTAGCTGcc                      >  1:922570/1‑100 (MQ=255)
                                                                          ctgcCCCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAg                    >  2:2198178/1‑100 (MQ=255)
                                                                          ctgcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAg                    >  1:837991/1‑100 (MQ=255)
                                                                          ctgcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAg                    >  1:769010/1‑100 (MQ=255)
                                                                          ctgcACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAg                    >  1:2513480/1‑100 (MQ=255)
                                                                           tgcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAgg                   <  1:1229207/100‑1 (MQ=255)
                                                                           tgcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAgg                   >  1:120638/1‑100 (MQ=255)
                                                                            gcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGc                  <  1:1605012/100‑1 (MQ=255)
                                                                               ccTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCAcc               >  2:448296/1‑100 (MQ=255)
                                                                               ccTCGTCCTTTGTGATCGTCCCGTCTTTTGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCAcc               <  1:530281/100‑1 (MQ=255)
                                                                                ctctTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCg              >  1:1523868/1‑100 (MQ=255)
                                                                                 tctTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGa             >  1:206642/1‑100 (MQ=255)
                                                                                  ctTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAg            <  1:491200/100‑1 (MQ=255)
                                                                                  ctTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAg            >  1:1413321/1‑100 (MQ=255)
                                                                                  ctTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAg            >  1:1384140/1‑100 (MQ=255)
                                                                                   ttCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAgc           >  1:992613/1‑100 (MQ=255)
                                                                                    tCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAgcg          <  2:780302/100‑1 (MQ=255)
                                                                                     ccGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAgcgc         >  2:1708495/1‑100 (MQ=255)
                                                                                      cGGTGTGAGTGTCCCGTCTTTGGTCCATTGCACGCGACCATCTTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGcgcg        <  1:2302283/100‑1 (MQ=255)
                                                                                       ggTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGa       >  1:2177448/1‑100 (MQ=255)
                                                                                        gtgtGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGAc      <  2:40001/100‑1 (MQ=255)
                                                                                        gtgtGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGAc      <  2:40021/100‑1 (MQ=255)
                                                                                         tgtgAGCGTCCCGTCTTTGGCCCATAGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACg     >  1:2363690/1‑100 (MQ=255)
                                                                                          gtgAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGc    >  1:1170786/1‑100 (MQ=255)
                                                                                           tgAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTTATCCAGCTGCCAGGCACCGAGCGCGACGcc   <  2:451745/100‑1 (MQ=255)
                                                                                           tgAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGcc   >  1:869570/1‑100 (MQ=255)
                                                                                            gAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGCCa  >  2:175126/1‑100 (MQ=255)
                                                                                                 |                                                                                              
AGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGCCA  >  NC_000913/4438670‑4438861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: