New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3561225100 (1.050)3 (0.030) 3/546 10.6 3.0% coding (228/831 nt) glpG rhomboid intramembrane serine protease
?NC_000913 = 3561234 99 (1.070)coding (219/831 nt) glpG rhomboid intramembrane serine protease
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATAAACACCACCACGCAGGCGATCATCATCACCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3560930‑3561225
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcaggccaTACCGGCAGTGGCCTGCATTATCGCCGTTATCCTTTCTTTGCCGCCTTGCGTGAACGCGCAGGTCCGGTAACCTGGGTGATGATGATCGCCTGCGTGGTGGTGTTTATTGCCATGCAAATTCTCGGCGATCAGGAAGTGATGTTATGGCTGGCCTGGCCATTCGATCCAACACTGAAATTTGAGTTCTGGCGTTACTTCACCCACGCGTTAATGCACTTCTCGCTGATGCATATCCTCTTTAACCTGCTCTGGTGGTGGTATCTCGGCGGTG  <  NC_000913/3561234‑3560963
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
TTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATAAACACCACCACGCAGGCGATCATCATCACCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATG                                                                                                                                                                                                                                                                                         <  1:813738/289‑1
                                                                       ATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATAAACACCACCACGCAGGCGATCATCATCACCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATG                                                                                                                                                                                                                                                                                         <  1:656213/218‑1
                                                                       ATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATAAACACCACCACGCAGGCGATCATCATCACCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATG                                                                                                                                                                                                                                                                                         >  2:656213/1‑218
                                                                                                                                                                                                                  CGCAGGCGATCATCATCACCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCATACCGGCAGTGGCCTGCATTATCGCCGTTATCCTTTCTTTGCCGCCTTGCGTGCACGCGCATGTCCGGTAACCTTGGTTATGATTATCGCCTGCGTGGTGGTGTTTATTGACATGCCAATTTTCGGCGATCAGTAAGTGATGTTATGGCTGGCCTGGCTATTCGATCTAACACTGAAATTTGAGTTCTGGCGTGACTTCACCC                                                                       >  2:245952/1‑289
                                                                                                                                                                                                                          ATCATCATCACCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCATACCGGCAGTGGCCTGCATTATCGCCGTTATCCTTTCTTTGCCGCCTTGCGTGAACGCGCAGGTCCGGTAACCTGGGTGATGATGATCGCCTGCGTGGTGGTGTTTATTGCCATGCAAATTCTCGGCGATCAGGAAGTGATGTTATGGCTGGCCTGGCCATTCGATCCAACACTGAAATTTGAGTTCTGGCGTTACTTCACCCACGCGTT                                                                >  1:355788/1‑288
                                                                                                                                                                                                                                                                                     cgagctgtgagtCAGGCCATACCGGCAGGGGCCTGCATTATCGCCGTTATCCTTTCTTTACCGCGTTGCGTGAACCCGCAGGTCCGGTAACCTGGGTGATTATGATCGCCTGGGTGGTGGTGTGTATTGCCATGCAAATTCTCGGCGATCAGGAAGTGATGTTATGGCTTGCCTGGCCATTCGATCCAACACTGAAATTTGAGTTCTGGCGTTACTTCACCCACGCGTTAATGCACTTCTCGCTGATGCATATCCTCTTTAACCTGCTCTGGTGGTGGTATCTCGGCGG    <  2:815591/277‑1
                                                                                                                                                                                                                                                                                       GGCGATAATGCAGGCCATACCGGCAGTGGCCTGCATTATCGCCGTTATCCTTTCTTTGCCGCCTTGCGTGAACGCGCAGGTCCGGTAACCTTGGTGATGATGATCGCCTGCGTGGTGGTGTTTATTGCCATGCAAATTCTCGGCGATCAGGAAGTGATGTTATGGCTGGCCTGGCCATTCGATCCAACACTGAAATTTGAGTTCTGGCGTTACTTCACCCACGCGTTAATGCACTTCTCGCTGATGCATATCCTCTTTAACCTGCTCTGGTGGTGGTATCTCGGCGGTG  <  1:245952/289‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
TTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATAAACACCACCACGCAGGCGATCATCATCACCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3560930‑3561225
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcaggccaTACCGGCAGTGGCCTGCATTATCGCCGTTATCCTTTCTTTGCCGCCTTGCGTGAACGCGCAGGTCCGGTAACCTGGGTGATGATGATCGCCTGCGTGGTGGTGTTTATTGCCATGCAAATTCTCGGCGATCAGGAAGTGATGTTATGGCTGGCCTGGCCATTCGATCCAACACTGAAATTTGAGTTCTGGCGTTACTTCACCCACGCGTTAATGCACTTCTCGCTGATGCATATCCTCTTTAACCTGCTCTGGTGGTGGTATCTCGGCGGTG  <  NC_000913/3561234‑3560963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.