Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,676	0	A	C	32.9%	155.4 / 16.2	70	V88G (GTG→GGG)	flc	fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base A (34/13);  new base C (0/23);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.45e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTC  >  NC_000913/657441‑657895                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              agagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTTTAACAAAAA‑cacc                                                                                                                                                                                                                             >  1:721247/1‑238 (MQ=255)      tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑c                                                                                                                                                                                                                       <  1:294834/238‑1 (MQ=255)       cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACt                                                                                                                                                                                                                          >  1:25480/1‑235 (MQ=255)       cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACt                                                                                                                                                                                                                          >  1:812249/1‑235 (MQ=255)             cACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGaaa                                                                                                                                                                                                               <  2:62648/239‑1 (MQ=255)                      tGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTgaga                                                                                                                                                                                                     >  1:612607/1‑239 (MQ=255)                       gCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGaa                                                                                                                                                                                                    <  1:1105751/239‑1 (MQ=255)                       gCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGaa                                                                                                                                                                                                    <  2:589536/239‑1 (MQ=255)                         cAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAgg                                                                                                                                                                                                  <  1:1352404/239‑1 (MQ=255)                                ccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTCCCGAACA‑TGATGAGAAGGTTGTTAg                                                                                                                                                                                           >  1:672292/1‑239 (MQ=255)                                   tCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGAcc                                                                                                                                                                                        >  1:362927/1‑239 (MQ=255)                                        tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGCCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACAACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGcctc                                                                                                                                                                                   >  2:16048/1‑237 (MQ=255)                                                          gTAAGAGACGACAGCAAGAATTCAAACCCCTCTT‑AAT‑CGGTGGT‑TTTTTTTT‑GGTTTTTAGGGGGGGGTG‑GAGGCCAAAAACGGCAAGAAGGCCGGTGCGGTTAGGGCAAAAGCCCCC‑AGAAGGTTGAAAAAATCGTTCAGTATTGCCAAACAAAAGCGGCCCTCTTG‑AAAAAAAA‑CCCCCCTTGCTGCCAAAAA‑TGTTGAGAAGGTTGTTAGGCCGCCACAAAATCCGGTGGTGATTa                                                                                                                                                                 <  2:612607/239‑1 (MQ=255)                                                                 aCGTCAGCAAGTATTAAAACCCCGCAT‑AAG‑C‑GGGGT‑ATTTTTTG‑GGTTCTTAAAGTGGGGTTATAGGCAAAAACCAACCAGAAACCCAGTGGGGTACTGGAAAAAACCCCC‑AGAAGTTTGACAAAAACTTTCAGTAATCCCCAACCAAGGCGCCCCGCGTGAAAAAACAC‑CCCCCCTT‑CTGCCAAAAT‑TTTTGAAAAGGTTGTAAAACCGCCACAAAATCCGGTGGTGATTAATACttt                                                                                                                                                          <  2:883001/239‑1 (MQ=255)                                                                   gTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTgag                                                                                                                                                                                                      <  2:305906/193‑1 (MQ=255)                                                                   gTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTgag                                                                                                                                                                                                      >  1:305906/1‑193 (MQ=255)                                                                   gTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGaa                                                                                                                                                                                                    >  2:413847/1‑195 (MQ=255)                                                                   gTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGaa                                                                                                                                                                                                    <  1:413847/195‑1 (MQ=255)                                                                   gTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTcc                                                                                                                                                        >  1:1598739/1‑239 (MQ=255)                                                                     cAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGa                                                                                                                                                                                                                 >  1:914352/1‑181 (MQ=255)                                                                     cAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGa                                                                                                                                                                                                                 <  2:914352/181‑1 (MQ=255)                                                                      cgaaaagaagtaaaaCCCGTTT‑AAA‑CCGGGGGTTTTTTTTT‑GGTCTATAGGGTGCGGTG‑GAGGCCAAAACCAGCCAGAAGGCCAGTGGGGTCAGGACAAAACCCCCC‑AGAAGGTTAAAAAAAACGTTCAGTAATGCCCAGCAAAGGCGGCCTTCTTAAAAAAAAAC‑CCCCCCTT‑CGGCAAAAAT‑TGTTGAGAGGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCca                                                                                                                                                       <  2:787426/227‑1 (MQ=255)                                                                        gaaagaataaaaaCCCCGTT‑AAA‑CAGGGGG‑TTTTTTTTGGGTGTTGAGTGTGGGGGA‑GAGCCCAAAAAAACCCAGAATGCCAGGGGGGTCAGGACAAAAGCCCCC‑AAAAGTTTAAAGAAAACGTGCAGTAATCCCACACCAAGGCGCCCCTTTTGTAAAAAAAA‑CCCCACTT‑CGGCCAAAAA‑TTTTGAGAGGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCacac                                                                                                                                                    <  2:539713/230‑1 (MQ=255)                                                                          aGAAATCAAAACCCGACT‑AGTACGGCGGG‑TTTTTTTG‑GTTTTTGAGTGGGCGTTG‑GTGGCGAAAACCACCCAGAATGCCAGTGCGTTAAGGAAAAAAGCCCCC‑AGAAGGTTGCCGAAGGCTTCCAGAAATCCCCAGCAAAGGCGGCCATATAGGAAAAAAAC‑CCCCACTT‑CTCCCAAAAA‑TTGTGAGAAGTGTTTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCcaca                                                                                                                                                     <  2:362927/236‑1 (MQ=255)                                                                            atttCAAACCCCGCTA‑AAT‑CAGCGGT‑TTTTTTTT‑GTTCTTTGTTGTGCGGTG‑GAGGCAAAAAACAGCAAGACTCCCAGTGCGGTAAGGCCAAAAGCCCCA‑AGAAGGTGAACGAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTTTTGAAAAAAAAA‑CCCCACTT‑CTCCCAAAAA‑TGTTGAAAGGGTTGTTAGAc                                                                                                                                                                                         <  2:656811/195‑1 (MQ=255)                                                                             aataaaaaCCCCCTTAAAA‑CGGGGGT‑TTTTTTTT‑GTTTTTTAGTGTGGGGTG‑GACGCCAAAAACAGCCAGAATGCCGGGGCGGTCAGGGAAAAAGCCCCC‑AAAAGGTTGAAGAAAACGTTCAGTAACGCCCAGCAAAGGCGGCCCTTTTAAAAAAAAAC‑CCCCCCTC‑CGGCGAAAAA‑TGTTGAGAGGTTTGTAAGACCGCCACAAAATCCggtg                                                                                                                                                                        <  2:462724/210‑1 (MQ=255)                                                                             aTTCAAAACCCGTGT‑AAT‑CGGCGGT‑TTTTTTTT‑GTTCTTTAGTGGGCGGTT‑GAGGCAAAAAACAGCCAGAATGCCAGTGCGGTAATGGCAAAAGCCCCC‑AGAAGGTAGACAAAAACGTCCAATAATGCCCAGCCAAAGCGGCCCTTTTGAAAAAAAAA‑CCCCCCTT‑CGGCCAAAAA‑TGTTGAGAAGTTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCcaca                                                                                                                                                     <  2:812249/232‑1 (MQ=255)                                                                                   aaCCCGCTT‑AAT‑CAGCGGG‑TTTTTTTC‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGt                                                                                                                                         >  1:1576251/1‑238 (MQ=255)                                                                                      ccGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACaaaa                                                                                                                                                                               >  1:656811/1‑197 (MQ=255)                                                                                      ccGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATa                                                                                                                                                              >  1:462724/1‑214 (MQ=255)                                                                                        attt‑AAT‑CAGGGTG‑TTTTTTTG‑GGTTTTTGGTGGGCGTTG‑GAGCCAGAAAACACCCAAAATGCCAGGGCTGTCGT‑GAAAAACACCCCAAGAAGGTTAAAAAAAACTTTCAGTAATGCCAAGCAAAAGCGCCCCTTTTAAAAAAAAAC‑CCCCCCTC‑CGGCCAAAAA‑TGTTGAAAATTTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCa                                                                                                                                   <  2:540264/237‑1 (MQ=255)                                                                                         cTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCa                                                                                                                                   >  1:278258/1‑238 (MQ=255)                                                                                                     ggg‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGTTCAATGTTCGTCATCCTGCTGaa                                                                                                                         >  1:1242646/1‑238 (MQ=255)                                                                                                     ggg‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGaa                                                                                                                         >  1:1100300/1‑238 (MQ=255)                                                                                                                               cGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTc                                                                                                                                       >  1:1288896/1‑200 (MQ=255)                                                                                                                                        gCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGcccc                                                                                        >  1:1076257/1‑239 (MQ=255)                                                                                                                                             aaaCAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCa                                                                                   >  1:894816/1‑239 (MQ=255)                                                                                                                                             aaaCAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCa                                                                                   >  1:751186/1‑239 (MQ=255)                                                                                                                                                   ccAAAATGCCGGTGCGGTCAGGGCAAAACCCCCC‑AGAAGGTAGAAGAAAACGTTCAATAATGCCCAGCAAAAGCGGCCCTCTTGAAAAAAAAA‑CACCCCTT‑CTGCCAAAAA‑TGTTGAGAAGGTTTTAAGACCGCCACAAAATCCGGTGGTGATTAAt                                                                                                                                                               <  2:1271916/157‑1 (MQ=255)                                                                                                                                                       aaTGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGttt                                                                              >  1:1260667/1‑234 (MQ=255)                                                                                                                                                            cgggggggTCATGGCAAAAGCCCCC‑AGAAGGTTAACAAAAACTTTCAGTATGGCCCAGCCAAAGCGGCCCTCTTGAAAAAAAAA‑CCCCCCTT‑CGGCAGAAAA‑TTTTGAAAGGGTTGTTGAACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGc                                                                     <  2:1209382/232‑1 (MQ=255)                                                                                                                                                             aGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCcaca                                                                                                                                                     >  1:1271916/1‑157 (MQ=255)                                                                                                                                                                 cGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTcccc                                                               >  1:520336/1‑239 (MQ=255)                                                                                                                                                                        ggaaaaaGCCCCA‑AGAAGTTGGAAAAAAACGTTAAGTAATGCCAAGCCAAAGCGGCCTTTTTGAAAAAAAAA‑CCCCACTT‑CTGCAGAAAA‑TGTTGAGAGGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGaa                                                        <  1:727944/236‑1 (MQ=255)                                                                                                                                                                             aaGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCg                                                     >  1:270566/1‑237 (MQ=255)                                                                                                                                                                                  cgc‑AGAGGGTAGACAAAAACGTCCAGTAACGCCCAGCAAAAGCGGCCCTCTTGTAAAAAAAA‑CCCCCCTT‑CTGCAGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAc                                                                                                                        <  2:1091714/163‑1 (MQ=255)                                                                                                                                                                                  ccc‑AGAAGGTTAAAAAAAACTTTCAGTAATCCCCAGCCAAAGCGGCCCTTTTGAAACAAAAC‑CCCCCCTT‑CTGCCAAAAA‑TGTTGAGAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATc                                                                                                                                 <  2:827627/156‑1 (MQ=255)                                                                                                                                                                                      aaaaGGTAGAAGAAAACGTTCAATAATGCCCAGCAAAAGGGGCCCTTTTGAAAAAAAAA‑CCCCCCTT‑CTCCAAAAAA‑TGTTGAGATGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAg                                           <  2:1598739/237‑1 (MQ=255)                                                                                                                                                                                            tgaaCGAAACCGTCCAGAAATCCCCACCCAAAGCGCCCCTCTTGAAAAAAAAA‑CCCCTTTT‑CTGCCAAAAA‑TGTTGAAAGGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAAt                                                                                                                                                               <  1:181784/114‑1 (MQ=255)                                                                                                                                                                                            agatagAATACTTTGAGAAATACCCAGCCAAACGGTCCTTTTTTTAAAAAAAC‑CACCATTT‑TTGCAAAAAA‑TTTTGAAAGGGTTGTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCa                                            <  2:1242646/227‑1 (MQ=255)                                                                                                                                                                                             tGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAcc                                                                                                                       >  1:827627/1‑156 (MQ=255)                                                                                                                                                                                             tGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtc                                                                                                              >  1:1091714/1‑165 (MQ=255)                                                                                                                                                                                             agACGAAACCTTTCAGTAATGCCCACCAAAGGCGGCCCTCTTGTAAAAAAAA‑CCCCACTT‑CTGCAGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGtt                                     <  1:16048/237‑1 (MQ=255)                                                                                                                                                                                               aCGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGaa                                                                                                                         >  1:308265/1‑152 (MQ=255)                                                                                                                                                                                                 aaaaaCGTTCAGAAACGCCCA‑CCAAAGGCGCCCCTTTGAAACAAAAACCCCCACTC‑CTGCCAAAAG‑TTTTAAGAAGGTTTTTGGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATc                                <  2:279107/238‑1 (MQ=255)                                                                                                                                                                                                    aaCGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCggtg                                                                                                                                                                        >  1:1406878/1‑100 (MQ=255)                                                                                                                                                                                                    aaCGTTCAGTAATGCCCAGCAAAGGCGTCCCTTTTATAAAAAAAA‑CCCCACTC‑CTGCCAAAAT‑TGTTGAGAAGGTTGTTGGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCa                             <  1:1248067/239‑1 (MQ=255)                                                                                                                                                                                                      cGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCcaca                                                                                                                                                     >  2:181784/1‑117 (MQ=255)                                                                                                                                                                                                        ttCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGa                                                                                                                                                                                                                 >  2:692257/1‑56 (MQ=255)                                                                                                                                                                                                        ttCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGa                                                                                                                                                                                                                 <  1:692257/56‑1 (MQ=255)                                                                                                                                                                                                           aGTAACCCCCAGCC‑AAGGGGCCCTTTTGGAAAAAAAA‑ACCCCCCT‑CCGGCGAAAATTGTTGAGAAGTTTTTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTc                                                                                                                                                         <  2:93951/108‑1 (MQ=255)                                                                                                                                                                                                               acgCCCAGCAAAAGCGGCTCTTTAAAAAAAAAAC‑CCCCTCTC‑CTGCAAAAAT‑TTTTGAAAGGTTTGTTACACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGc                  <  2:520336/237‑1 (MQ=255)                                                                                                                                                                                                                     aGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGAt                                                                                                                                               >  1:93951/1‑108 (MQ=255)                                                                                                                                                                                                                       ccAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCg           >  1:961749/1‑238 (MQ=255)                                                                                                                                                                                                                       caaaaGCGCCCCTTTTAAAACAAAAC‑CCCCTCTT‑CGGCCAAAAA‑TGTTGAAAAGGTTTTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGc                                                                     <  2:1196434/178‑1 (MQ=255)                                                                                                                                                                                                                           accGGCCCTTTTGTAAAAAAAA‑CCCCACTT‑CTGCAGAAAA‑TTTTGAAAGGTTTGTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACg      <  2:270566/237‑1 (MQ=255)                                                                                                                                                                                                                           aGCGGCCTTCTTGAAAAAAAAC‑CCCCCCTC‑CGGCCGAAAA‑TGTTAAGAGGTTTATTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACg      <  2:1076257/239‑1 (MQ=255)                                                                                                                                                                                                                             caccccTGTTGAAAAAAAAA‑CACCACTC‑CTGCCAAAAA‑TGATAAGAAGGTTTTGAGCCCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACg      <  2:25480/234‑1 (MQ=255)                                                                                                                                                                                                                              cgcctttttGAAACAAAAC‑CACCACTC‑CGGCAGAAAA‑GTGTGAGAGGTTTGTAAACCCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCt    <  2:1100300/231‑1 (MQ=255)                                                                                                                                                                                                                                cccTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTc  >  1:528813/1‑238 (MQ=255)                                                                                                                                                                                                                                 ccTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACg                                                           >  1:1196434/1‑180 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              AGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT‑AAT‑CAGCGGG‑TTTTTTTT‑GGTCTTTAGTGTGCGGTT‑GAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCC‑AGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAA‑CACCACTT‑CTGCCGAAAA‑TGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTC  >  NC_000913/657441‑657895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑