breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 108 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6860GT51.5% ‑5.0 / 22.3 33intergenic (+40/+25)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_0009132,151,6890GT51.5% ‑5.0 / 15.1 33intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_000913417,5330TG47.3% 39.0 / 17.8 56V131G (GTG→GGG) phoBresponse regulator in two‑component regulatory system with PhoR
*NC_0009133,862,6610TG35.9% 34.4 / 10.7 42pseudogene (943/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_000913433,6490TG34.2% 110.5 / 27.7 115G65G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*NC_000913753,1250TA32.5% 171.6 / 43.7 83intergenic (‑330/+60)ybgD/gltAputative fimbrial‑like adhesin protein/citrate synthase
*NC_0009133,862,6770GA31.2% 8.9 / 12.2 39pseudogene (927/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_0009134,429,9840TG30.8% 242.3 / 23.0 107L41V (TTG→GTG) cycAD‑alanine/D‑serine/glycine transporter
*NC_000913433,6340TG30.2% 148.8 / 20.5 123G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*NC_0009134,429,9900AT29.7% 243.2 / 42.0 113M43L (ATG→TTG) cycAD‑alanine/D‑serine/glycine transporter
*NC_0009133,844,2190TG29.5% 286.4 / 11.4 122F86V (TTT→GTT) adeDcryptic adenine deaminase
*NC_0009132,608,2440TG29.3% 161.6 / 14.3 99V443G (GTG→GGG) hyfFhydrogenase 4, membrane subunit
*NC_0009133,745,0190CG29.0% 85.1 / 17.7 69G72G (GGC→GGGyiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
*NC_0009132,151,6760CG28.2% 98.6 / 14.0 39intergenic (+30/+35)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_000913599,5790TG27.8% 257.7 / 19.4 108V289G (GTG→GGG) cusAcopper/silver efflux system, membrane component
*NC_0009131,876,0150AG27.7% 259.5 / 29.4 114I115V (ATT→GTT) yeaNputative MFS transporter, inner membrane protein
*NC_0009131,658,1670GT27.5% 154.8 / 16.7 70L33F (TTG→TTTynfEputative selenate reductase, periplasmic
*NC_0009134,296,0600CT27.5% 334.5 / 191.0 218intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009134,098,3910AC27.3% 260.6 / 25.7 131Y219S (TAC→TCC) rhaStranscriptional activator of rhaBAD and rhaT
*NC_0009132,151,7100CA27.3% 97.7 / 12.6 44intergenic (+64/+1)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein

Marginal new junction evidence (lowest skew 10 of 20 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =241 (1.190)36 (0.190) 31/324 4.3 14.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 194 (1.050)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805227 (1.120)39 (0.210) 27/324 4.9 16.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 189 (1.020)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 2765420 =271 (1.330)8 (0.050) 7/268 9.2 4.2% intergenic (‑267/+93) yfjL/yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage; uncharacterized protein
?NC_000913 = 2765411 158 (1.040)intergenic (‑258/+102) yfjL/yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage; uncharacterized protein
* ? NC_000913 4289016 =228 (1.120)8 (0.040) 8/338 10.2 3.6% coding (1253/1437 nt) nrfA nitrite reductase, formate‑dependent, cytochrome
?NC_000913 4289092 = 208 (1.080)coding (1329/1437 nt) nrfA nitrite reductase, formate‑dependent, cytochrome
* ? NC_000913 = 2331858209 (1.030)7 (0.040) 7/340 10.7 3.4% pseudogene (2545/4605 nt) yfaS pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
?NC_000913 = 2331859 196 (1.010)pseudogene (2544/4605 nt) yfaS pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
* ? NC_000913 = 454269069 (0.340)5 (0.030) 5/338 11.6 4.9% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 129 (0.670)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 1082864 =227 (1.120)5 (0.030) 5/336 11.6 2.5% coding (622/1128 nt) efeO inactive ferrous ion transporter EfeUOB
?NC_000913 1083032 = 183 (0.960)coding (790/1128 nt) efeO inactive ferrous ion transporter EfeUOB
* ? NC_000913 = 3010000198 (0.980)6 (0.030) 5/340 11.7 3.1% intergenic (+25/‑28) ygeY/hyuA putative peptidase/D‑stereospecific phenylhydantoinase
?NC_000913 = 3010004 182 (0.940)intergenic (+29/‑24) ygeY/hyuA putative peptidase/D‑stereospecific phenylhydantoinase
* ? NC_000913 = 1946034186 (0.920)6 (0.030) 5/340 11.7 3.3% intergenic (‑58/+81) ruvA/yobI component of RuvABC resolvasome, regulatory subunit/uncharacterized protein
?NC_000913 = 1946036 178 (0.920)intergenic (‑60/+79) ruvA/yobI component of RuvABC resolvasome, regulatory subunit/uncharacterized protein
* ? NC_000913 3515982 =209 (1.030)5 (0.030) 4/338 12.2 2.4% intergenic (‑69/+38) dam/damX DNA adenine methyltransferase/cell division protein that binds to the septal ring
?NC_000913 3516010 = 201 (1.040)intergenic (‑97/+10) dam/damX DNA adenine methyltransferase/cell division protein that binds to the septal ring