breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 76,539 T→G 6.4% E254A (GAA→GCA)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 708,109 A→G 5.3% intergenic (+352/‑225) glnS → / → chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
RA 930,957 A→C 5.7% N2K (AAT→AAG cydD ← glutathione/cysteine ABC transporter export permease/ATPase
RA 1,269,450 C→T 5.3% intergenic (+61/+253) rdlA → / ← ldrB sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA/toxic polypeptide, small
RA 1,269,612 T→A 5.2% intergenic (+223/+91) rdlA → / ← ldrB sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA/toxic polypeptide, small
JC JC 1,292,997 IS2 (–) +5 bp 70.2% intergenic (‑75/‑526) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
JC JC 1,293,004 IS1 (–) +9 bp 72.0% intergenic (‑82/‑515) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
JC JC 1,293,074 IS1 (+) +9 bp 16.5% intergenic (‑152/‑445) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
JC JC 1,293,196 IS5 (+) +4 bp 13.1% intergenic (‑274/‑328) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,343,516 A→C 5.5% intergenic (‑188/+81) osmB ← / ← yciT osmotically and stress inducible lipoprotein/global regulator of transcription; DeoR family
RA 1,466,210 G→A 6.2% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,604 G→A 8.5% pseudogene (1213/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,729 G→A 5.3% pseudogene (1338/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,703,098 A→C 31.1% H289P (CAC→CCC)  add → adenosine deaminase
RA 1,708,930 C→A 12.6% P671Q (CCG→CAG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
JC JC 2,052,434 IS1 (–) +9 bp 13.6% pseudogene (887‑895/1053 nt) yeeL ← pseudogene, glycosyltransferase homology
RA 2,075,512 T→G 5.3% L245R (CTG→CGG)  yeeR → CP4‑44 prophage; putative membrane protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
JC JC 2,224,288 IS5 (+) +4 bp 8.9% coding (580‑583/933 nt) pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,323,353 T→G 8.4% intergenic (+102/‑94) atoC → / → atoD fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
RA 2,649,866 G→C 5.8% L808V (CTG→GTG)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,809,761 G→T 6.8% E49* (GAA→TAA)  ygaZ → putative L‑valine exporter, norvaline resistance protein
RA 3,006,859 A→C 100% S200R (AGC→CGC)  ygeW → putative carbamoyltransferase
RA 3,233,264 A→G 7.7% I534V (ATC→GTC)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,577,196 T→G 5.8% M133L (ATG→CTG)  gntK ← gluconate kinase 2
RA 3,697,854 A→C 5.5% Q466P (CAA→CCA)  bcsE → cellulose production protein
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
JC 4,001,527 +GACGAC 34.5% coding (102/951 nt) corA → magnesium/nickel/cobalt transporter
JC 4,001,538 Δ99 bp 15.3% coding (113‑211/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,002,011 C→T 27.7% Q196* (CAG→TAG)  corA → magnesium/nickel/cobalt transporter
RA 4,002,319:1 +A 8.6% coding (894/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,183,258 G→A 100% E672K (GAA→AAA)  rpoB → RNA polymerase, beta subunit
RA 4,296,190 A→G 21.6% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 23.3% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 17.1% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 5.4% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 381689NA (NA)8 (0.060) 6/530 NT NA noncoding (430/1331 nt) IS2 repeat region
?NC_000913 = 381743 NA (NA)noncoding (484/1331 nt) IS2 repeat region
* ? NC_000913 1207790 =75 (0.540)77 (0.590) 57/516 NT 56.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 50 (0.380)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780572 (0.520)90 (0.690) 58/516 NT 60.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 50 (0.380)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)94 (0.690) 60/540 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1630598 =90 (0.650)9 (0.060) 9/548 NT 9.1% coding (316/1461 nt) ydfI putative NAD‑dependent D‑mannonate oxidoreductase
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 19785020 (0.000)79 (0.590) 59/532 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein