Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,798,659 (T)8→7 coding (654/1074 nt) waaU ← lipopolysaccharide core biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,798,6520T.94.6% 139.8 / 3.2 37coding (661/1074 nt)waaUlipopolysaccharide core biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (20/15);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

ACAGAAATGACAAAATCACTATACTTGGTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAATAGTAACGCTTATACCAATGATCA  >  NC_000913/3798456‑3798875
                                                                                                                                                                                                    |                                                                                                                                                                                                                               
aCAGAAATGACAAAATCACTATACTTGGTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTaa                                                                                                                                                                                                                  >  1:777779/1‑212 (MQ=255)
aCAGAAATGACAAAATCACTATACTTGGTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTaa                                                                                                                                                                                                                  <  2:777779/212‑1 (MQ=255)
                          ggTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTc                                                                                                                                                            >  2:624850/1‑239 (MQ=255)
                          ggTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑‑TTTTTTGCACCTAATGGa                                                                                                                                                                                                              >  2:619442/1‑188 (MQ=255)
                          ggTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATA‑‑TTTTTTGCACCTAATGGa                                                                                                                                                                                                              <  1:619442/188‑1 (MQ=255)
                                                 aCTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTACTAATGa                                                                                                                                                                                                    >  2:526218/1‑176 (MQ=255)
                                                 aCTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGa                                                                                                                                                                                                    <  1:526218/176‑1 (MQ=255)
                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTaataat                                                                                                                                                                                                      >  2:915810/1‑173 (MQ=255)
                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTaataat                                                                                                                                                                                                      >  2:35187/1‑173 (MQ=255)
                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTaataat                                                                                                                                                                                                      <  1:915810/173‑1 (MQ=255)
                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTaataat                                                                                                                                                                                                      <  1:35187/173‑1 (MQ=255)
                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTAtt                                                                                                                                                                                               >  1:275523/1‑180 (MQ=255)
                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTAtt                                                                                                                                                                                               <  2:275523/180‑1 (MQ=255)
                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCCATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTc                                                                                                                                    >  2:896233/1‑239 (MQ=255)
                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAAt                                                                                                                                           >  1:893502/1‑232 (MQ=255)
                                                  cTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAAt                                                                                                                                           <  2:893502/232‑1 (MQ=255)
                                                     atcaAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGa                                                                                                                                                                                                              >  2:808920/1‑162 (MQ=255)
                                                     atcaAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGa                                                                                                                                                                                                              <  1:808920/162‑1 (MQ=255)
                                                                       gATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTAcc                                                                                                               <  1:624850/239‑1 (MQ=255)
                                                                        aTTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTAcc                                                                                                               >  2:147311/1‑238 (MQ=255)
                                                                                    ggtattgGCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAata                                                                                                  <  2:187845/239‑1 (MQ=255)
                                                                                          gTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTaa                                                                                                                                                                                                                  >  2:242976/1‑121 (MQ=255)
                                                                                          gTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTaa                                                                                                                                                                                                                  <  1:242976/121‑1 (MQ=255)
                                                                                                       gCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCt                                                                                                                                                                                                                    >  1:631206/1‑106 (MQ=255)
                                                                                                       gCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCt                                                                                                                                                                                                                    <  2:631206/106‑1 (MQ=255)
                                                                                                       gCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTg                                                                               <  1:147311/239‑1 (MQ=255)
                                                                                                                            tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATaaa                                                                                                    <  2:415439/197‑1 (MQ=255)
                                                                                                                            tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATaaa                                                                                                    >  1:415439/1‑197 (MQ=255)
                                                                                                                                               tgtTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTaa                                                                                                                                                                                                                  <  2:626856/68‑1 (MQ=255)
                                                                                                                                               tgtTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTaa                                                                                                                                                                                                                  >  1:626856/1‑68 (MQ=255)
                                                                                                                                                       cttcttGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTaaa                               >  1:926789/1‑239 (MQ=255)
                                                                                                                                                             gATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAAt                         >  2:56866/1‑239 (MQ=255)
                                                                                                                                                                  ataACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTTATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAATAGTa                     >  2:849091/1‑238 (MQ=255)
                                                                                                                                                                         ttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAATAGTAACGCTtat             >  1:105655/1‑239 (MQ=255)
                                                                                                                                                                                    aaCGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCACCGTGGGGATGGTAAAATGAATAGTAACGCTTATACCAATGATcc  >  2:813557/1‑238 (MQ=255)
                                                                                                                                                                                               gCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATaaa                                                                                                                                                                              >  2:264606/1‑56 (MQ=255)
                                                                                                                                                                                               gCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATaaa                                                                                                                                                                              <  1:264606/56‑1 (MQ=255)
                                                                                                                                                                                                    |                                                                                                                                                                                                                               
ACAGAAATGACAAAATCACTATACTTGGTCAACGCAACGGTATAAATAAACTCATCAAATGGTAGTGTTTCGATTTCAAGTATTGGTATTGTCAATAAATCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCGTAGTTGGTGGAAAATTTACCCTCACCATAAATATGCTTCAGTATTTCTATTGCTCGTGTACTCATATGTTCTTTTAAACATTCATCGTGGGGATGGTAAAATGAATAGTAACGCTTATACCAATGATCA  >  NC_000913/3798456‑3798875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: