Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	354,812	C→T	intergenic (+220/‑110)	prpE → / → codB	propionate‑‑CoA ligase/cytosine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,812	0	C	T	100.0%	244.7 / NA	62	intergenic (+220/‑110)	prpE/codB	propionate‑‑CoA ligase/cytosine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/29);  total (33/29)

GATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCC  >  NC_000913/354603‑355026                                                                                                                                                                                                                  |                                                                                                                                                                                                                       gATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCAc                                                                                                                                                                                           >  2:117789/1‑239 (MQ=255)                                gCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGaa                                                                                                                                                             <  2:917988/238‑1 (MQ=255)                                     tGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAAt                                                                                                                                                       >  2:65006/1‑239 (MQ=255)                                       ccTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAACAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGACTAGAAtac                                                                                                                                                     >  2:600000/1‑237 (MQ=255)                                       ccTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTc                                                                                                                                                                                                >  2:20105/1‑196 (MQ=255)                                       ccTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTc                                                                                                                                                                                                <  1:20105/196‑1 (MQ=255)                                       ccTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATgc                                                                                                                                                     >  1:104670/1‑239 (MQ=255)                                            tGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTcccc                                                                                                                                                                                             >  2:539478/1‑194 (MQ=255)                                            tGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTcccc                                                                                                                                                                                             <  1:539478/194‑1 (MQ=255)                                                    tGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttttt                                                                                                                                         <  1:65006/238‑1 (MQ=255)                                                             ttATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTCTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCaaa                                                                                                                               <  1:812242/239‑1 (MQ=255)                                                              tATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATGTAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAAc                                                                                                                              <  2:717917/239‑1 (MQ=255)                                                                tCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt                                                                                                                                                                     >  1:273743/1‑198 (MQ=255)                                                                tCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt                                                                                                                                                                     <  2:273743/198‑1 (MQ=255)                                                                         cGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGc                                                                                                                           <  1:160660/231‑1 (MQ=255)                                                                         cGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGc                                                                                                                           >  2:160660/1‑231 (MQ=255)                                                                         cGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAggg                                                                                                                   >  1:153307/1‑239 (MQ=255)                                                                               tGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCatat                                                                                                                                                                                                              >  2:884765/1‑142 (MQ=255)                                                                               tGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCatat                                                                                                                                                                                                              <  1:884765/142‑1 (MQ=255)                                                                                gAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAg                                                                                                                                                                  <  2:320539/185‑1 (MQ=255)                                                                                gAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAg                                                                                                                                                                  >  1:320539/1‑185 (MQ=255)                                                                                 aaCCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtca                                                                                                                                                                                                                     <  1:319479/133‑1 (MQ=255)                                                                                 aaCCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtca                                                                                                                                                                                                                     >  2:319479/1‑133 (MQ=255)                                                                                      tAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCAtata                                                                                                                                                                                                             >  1:751035/1‑136 (MQ=255)                                                                                      tAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCAtata                                                                                                                                                                                                             <  2:751035/136‑1 (MQ=255)                                                                                       aGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCg                                                                                                     <  2:104670/239‑1 (MQ=255)                                                                                        ggTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt                                                                                                                                                                     >  1:837452/1‑174 (MQ=255)                                                                                        ggTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt                                                                                                                                                                     <  2:837452/174‑1 (MQ=255)                                                                                                aaGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaa                                                                                             <  2:153307/238‑1 (MQ=255)                                                                                                   gcgcTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttttt                                                                                                                                         >  1:190320/1‑191 (MQ=255)                                                                                                   gcgcTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttttt                                                                                                                                         <  2:190320/191‑1 (MQ=255)                                                                                                        cgcgTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTc                                                                                                                                                                                                >  2:573320/1‑131 (MQ=255)                                                                                                        cgcgTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTc                                                                                                                                                                                                <  1:573320/131‑1 (MQ=255)                                                                                                        cgcgTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTggg                                                                                                                                      >  1:481880/1‑189 (MQ=255)                                                                                                        cgcgTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTggg                                                                                                                                      <  2:481880/189‑1 (MQ=255)                                                                                                        cgcgTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCACAAAGGGGGAATTTCGTGTCGCAAGTTAACAACTTTa                                                                                     >  2:341587/1‑238 (MQ=255)                                                                                                              gCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt                                                                                                                                                                     >  2:640655/1‑152 (MQ=255)                                                                                                              gCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt                                                                                                                                                                     <  1:640655/152‑1 (MQ=255)                                                                                                                          aTGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCa                                                                                                                                 >  2:938637/1‑176 (MQ=255)                                                                                                                          aTGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCa                                                                                                                                 <  1:938637/176‑1 (MQ=255)                                                                                                                           tGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTccc                                                                     >  1:514641/1‑235 (MQ=255)                                                                                                                           tGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCa                                                                  >  1:529884/1‑238 (MQ=255)                                                                                                                                  aTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCAtata                                                                                                                                                                                                             <  1:623943/92‑1 (MQ=255)                                                                                                                                  aTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCAtata                                                                                                                                                                                                             >  2:623943/1‑92 (MQ=255)                                                                                                                                  aTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGgcgc                                                          >  2:757312/1‑239 (MQ=255)                                                                                                                                   tGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCGTTCATATAAAAAATATATTTCCCCACGAAAACGAt                                                                                                                                                                                  <  1:55552/118‑1 (MQ=255)                                                                                                                                   tGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCGTTCATATAAAAAATATATTTCCCCACGAAAACGAt                                                                                                                                                                                  >  2:55552/1‑118 (MQ=255)                                                                                                                                              aCGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTcccc                                                                                                                                                                                             <  1:176067/96‑1 (MQ=255)                                                                                                                                              aCGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTcccc                                                                                                                                                                                             >  2:176067/1‑96 (MQ=255)                                                                                                                                                                  gCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCa                                                                                 >  1:371755/1‑184 (MQ=255)                                                                                                                                                                  gCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCa                                                                                 <  2:371755/184‑1 (MQ=255)                                                                                                                                                                  gCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCAt                           >  2:47718/1‑238 (MQ=255)                                                                                                                                                                   ccTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGc                         >  1:602688/1‑239 (MQ=255)                                                                                                                                                                            tGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAg                                                                                                                                                                  <  2:680294/93‑1 (MQ=255)                                                                                                                                                                            tGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAg                                                                                                                                                                  >  1:680294/1‑93 (MQ=255)                                                                                                                                                                                      ccGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt                                                                                                                                                                     >  1:763402/1‑80 (MQ=255)                                                                                                                                                                                      ccGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt                                                                                                                                                                     <  2:763402/80‑1 (MQ=255)                                                                                                                                                                                        gATAATTTTTCCCCCACCTTTTTGCATtcattcat                                                                                                                                                                                                                >  1:285767/1‑35 (MQ=255)                                                                                                                                                                                        gATAATTTTTCCCCCACCTTTTTGCATtcattcat                                                                                                                                                                                                                <  2:285767/35‑1 (MQ=255)                                                                                                                                                                                          tAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGcc  <  1:47718/239‑1 (MQ=255)                                                                                                                                                                                                        ccTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTa                                               <  1:929484/180‑1 (MQ=255)                                                                                                                                                                                                        ccTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTa                                               >  2:929484/1‑180 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                       GATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCC  >  NC_000913/354603‑355026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑