Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,798,659 (T)8→7 coding (654/1074 nt) waaU ← lipopolysaccharide core biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,798,6520T.90.6% 587.4 / 57.9 159coding (661/1074 nt)waaUlipopolysaccharide core biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (10/5);  new base . (69/75);  total (79/80)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

TCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCG  >  NC_000913/3798555‑3798746
                                                                                                 |                                                                                              
tCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGGTATATAACTTTTATTTGCTCAAACGTAAGACGGCATCtt                                                                                               >  1:2439963/1‑99 (MQ=255)
  ttGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttt                                                                                             >  1:3243657/1‑99 (MQ=255)
   tGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAttttt                                                                                            <  2:2924273/99‑1 (MQ=255)
   tGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGGTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAttttt                                                                                            <  1:1052242/99‑1 (MQ=255)
    gCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttt                                                                                           <  1:2016552/99‑1 (MQ=255)
     cGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAttttttt                                                                                          <  2:2463838/99‑1 (MQ=255)
       gTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttttgc                                                                                        >  1:2689162/1‑97 (MQ=255)
        tAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttttgca                                                                                       >  1:3054319/1‑96 (MQ=255)
         aaCCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttttgcac                                                                                      <  2:347458/99‑5 (MQ=255)
         aaCCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATAtttttttgcac                                                                                      <  2:76864/99‑5 (MQ=255)
          aCCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCAcc                                                                                    <  1:105637/99‑1 (MQ=255)
             cGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTaa                                                                                 <  2:3299370/99‑1 (MQ=255)
             cGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTaa                                                                                 <  1:1137279/99‑1 (MQ=255)
              ggTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAAt                                                                                >  1:1748822/1‑99 (MQ=255)
                     ataATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATtaa                                                                         >  1:1875633/1‑99 (MQ=255)
                       aaTCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATtaata                                                                       >  2:3111678/1‑99 (MQ=255)
                       aaTCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATtaata                                                                       >  1:988331/1‑99 (MQ=255)
                         tCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTaataat                                                                     <  1:2228992/99‑1 (MQ=255)
                          ccGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATg                                                                    >  1:3277952/1‑99 (MQ=255)
                           cGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGa                                                                   <  2:2448131/99‑1 (MQ=255)
                           cGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGa                                                                   <  1:2334346/99‑1 (MQ=255)
                             aTAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACt                                                                 >  1:2807425/1‑99 (MQ=255)
                               aaTTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTAt                                                               >  1:921755/1‑99 (MQ=255)
                                 ttttCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGTACCTACTGTATTAATAATGACTATTc                                                             >  2:1593468/1‑99 (MQ=255)
                                 ttttCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTc                                                             >  1:2875306/1‑99 (MQ=255)
                                  tttCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCt                                                            >  2:2651091/1‑99 (MQ=255)
                                   ttCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCtt                                                           <  1:1634460/99‑1 (MQ=255)
                                   ttCAAAGTGTGTTTGCACTTCTTGATGTGTAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTCACCTAATGGATTAATAATGACTATTCtt                                                           <  1:1278684/99‑1 (MQ=255)
                                    tCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCtt                                                           <  1:3086253/99‑1 (MQ=255)
                                    tCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTg                                                          >  2:936062/1‑99 (MQ=255)
                                    tCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTg                                                          <  2:1260831/99‑1 (MQ=255)
                                     cAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGt                                                         <  2:2203596/99‑1 (MQ=255)
                                      aaaGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTa                                                        >  1:474620/1‑99 (MQ=255)
                                       aaGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTAt                                                       <  1:647651/99‑1 (MQ=255)
                                         gtgtgtTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTAtct                                                     >  1:1962188/1‑99 (MQ=255)
                                              ttttCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAAt                                                >  1:272057/1‑99 (MQ=255)
                                              ttttCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAAt                                                >  2:2047881/1‑99 (MQ=255)
                                              ttttCACTTCTTGATATATAACTTGTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAAt                                                <  2:753868/99‑1 (MQ=255)
                                                ttCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATaa                                              >  2:626670/1‑99 (MQ=255)
                                                   aCTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAAtt                                           >  2:1986042/1‑99 (MQ=255)
                                                    cttcttGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTc                                          <  2:1777603/99‑1 (MQ=255)
                                                    cttcttGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTc                                          >  1:2516180/1‑99 (MQ=255)
                                                     ttcttGATATATAACTTTTATGTGCTCAAACGTAATACGGCATATTTTTTTTGATCTAATGGATTAATAATGTCTATTTTTTTATCTCCATTAAATTCt                                         >  2:1555953/1‑99 (MQ=255)
                                                        ttGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCtttt                                      <  1:1811216/99‑1 (MQ=255)
                                                          gATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttat                                    <  1:1489539/99‑1 (MQ=255)
                                                           atatatAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttatt                                   <  2:1322139/99‑1 (MQ=255)
                                                            tatataACTTTTATTTGCTCAAACGTAAGACGACATATTTTTTTGCAACTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATACttttattt                                  <  2:286946/99‑1 (MQ=255)
                                                             atataACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttatttt                                 <  1:2923929/99‑1 (MQ=255)
                                                              tataACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCttttatttta                                <  2:1340635/99‑1 (MQ=255)
                                                               ataACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTtttattttat                               >  1:840509/1‑99 (MQ=255)
                                                               ataACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTtttattttat                               <  2:2761283/99‑1 (MQ=255)
                                                                taACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTtttattttat                               >  2:3009840/1‑99 (MQ=255)
                                                                taACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTtttattttat                               <  1:311735/99‑1 (MQ=255)
                                                                taACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATTGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATc                              >  2:2246806/1‑99 (MQ=255)
                                                                 aaCTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTTTTTTTTTTGATTAATAATGACTATTCTTGTATCTCCATTTAATTCTtttattttattt                             >  2:3349695/1‑97 (MQ=255)
                                                                 aaCTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCt                             >  1:224320/1‑99 (MQ=255)
                                                                  aCTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCtt                            <  2:1718088/99‑1 (MQ=255)
                                                                  aCTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCtt                            >  2:1664434/1‑99 (MQ=255)
                                                                  aCTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCtt                            >  1:2582779/1‑99 (MQ=255)
                                                                  aCTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCtt                            >  1:3409794/1‑99 (MQ=255)
                                                                  aCTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCtt                            >  1:1429984/1‑99 (MQ=255)
                                                                   cTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATGTCCAATAAATTCTTTTATTTTATCTTc                           >  1:3014961/1‑99 (MQ=255)
                                                                   cTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTc                           <  1:71975/99‑1 (MQ=255)
                                                                    ttttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCa                          >  2:1846367/1‑99 (MQ=255)
                                                                    ttttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCa                          >  2:2804756/1‑99 (MQ=255)
                                                                    ttttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCa                          >  1:627578/1‑99 (MQ=255)
                                                                    ttttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATATTCa                          >  1:802367/1‑99 (MQ=255)
                                                                    ttttATTTGCTCAAACGTAAGAAGTCATATTTTTTTGAACCTACTGGATTATTTATGCCTATTCTTGTAGCTCCAATAAATTCTTTTATTTTATCttaa                          <  2:2425394/99‑3 (MQ=255)
                                                                     tttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCaa                         <  2:1604997/99‑1 (MQ=255)
                                                                      ttATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCCCCAATAAATTCTTTTATTTTATCTTCAAc                        <  2:1902553/99‑1 (MQ=255)
                                                                       tATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACa                       <  2:2140999/99‑1 (MQ=255)
                                                                       tATTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACa                       <  2:458810/99‑1 (MQ=255)
                                                                        aTTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACAt                      >  1:1739787/1‑99 (MQ=255)
                                                                        aTTTGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACAt                      <  2:849060/99‑1 (MQ=255)
                                                                         tttGCTCAAACGTAAGACGGCATATTTTTTTTTTTTTTTTTTATTAATAATGACTATTCTTGTATCTCTTATTAATTCTTTTTTTTTATCTTCAACCTc                     >  2:2517622/1‑99 (MQ=255)
                                                                         tttGCTCAAACGTAAGACGGCATATTTTTTTTCATTTTTTGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATc                     >  2:524562/1‑99 (MQ=255)
                                                                         tttGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATc                     >  2:3057728/1‑99 (MQ=255)
                                                                         tttGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATc                     <  1:1191791/99‑1 (MQ=255)
                                                                          ttGCTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCt                    >  1:323357/1‑99 (MQ=255)
                                                                             gtCAAACGTACGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACt                 >  2:3171825/2‑99 (MQ=255)
                                                                             cTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACt                 <  1:876105/99‑1 (MQ=255)
                                                                             cTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACt                 <  2:2173013/99‑1 (MQ=255)
                                                                             cTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACt                 <  2:1169879/99‑1 (MQ=255)
                                                                             cTCAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACt                 <  1:3451719/99‑1 (MQ=255)
                                                                             cTCAAACGTAAGACGGCATATTCTTTTGTACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACt                 <  1:3164453/99‑1 (MQ=255)
                                                                              ttaaaCGTAAGACGGCATATTTTTTTGCATCTAATGGTTTATTAATGACTATTTTTGTATTTTCAATTAATTATTTTATTTTATTTTTAATATTTATTg                >  1:2454962/3‑99 (MQ=255)
                                                                              tCAAACGTAAGACGGCATATTTTTTTGCACCTAATGACTTAATAATGACTATTCTTGTACCTCACATAAATTTTTTTGTTTTACCTTCATCCTCTACTg                >  2:2894142/1‑99 (MQ=255)
                                                                               cAAACGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTgg               >  1:1946273/1‑99 (MQ=255)
                                                                                aaaCGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGt              <  1:2729472/99‑1 (MQ=255)
                                                                                aaaCGTAAGACGGCATATGTTTTTACACCTACTCGCTTAATAACGACTAATCTTGTACCTCCAATAACCTCTTTTATTCTATCTTCAACATCTACtcat              >  2:1051192/1‑96 (MQ=255)
                                                                                 aaCGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTa             >  1:2162880/1‑99 (MQ=255)
                                                                                  aCGTAAGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTaa            >  2:679834/1‑99 (MQ=255)
                                                                                      aaGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGc        >  1:2098329/1‑99 (MQ=255)
                                                                                       aGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCa       >  2:956877/1‑99 (MQ=255)
                                                                                       aGACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCa       >  1:1703028/1‑99 (MQ=255)
                                                                                        gACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCaa      <  2:493553/99‑1 (MQ=255)
                                                                                        gACGGCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCaa      >  1:3282292/1‑99 (MQ=255)
                                                                                           ggCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTc   <  1:2415138/99‑1 (MQ=255)
                                                                                            gCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCg  >  2:3152254/1‑99 (MQ=255)
                                                                                            gCATATTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGCCAATGCAAGTCg  >  1:2622867/1‑99 (MQ=255)
                                                                                                 |                                                                                              
TCTTGCGGTAACCCGGTAAATATAATCCGATAATTTTCAAAGTGTGTTTTCACTTCTTGATATATAACTTTTATTTGCTCAAACGTAAGACGGCATATTTTTTTTGCACCTAATGGATTAATAATGACTATTCTTGTATCTCCAATAAATTCTTTTATTTTATCTTCAACATCTACTGGTAAATGCAAGTCG  >  NC_000913/3798555‑3798746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: