Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,774,470 A→G M385V (ATG→GTG)  yfjW → CP4‑57 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,774,4700AG98.3% 418.1 / ‑2.9 121M385V (ATG→GTG) yfjWCP4‑57 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (70/48);  total (73/48)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 123 total aligned reads displayed.

ATGATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGATGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAACCAGA  >  NC_000913/2774378‑2774564
                                                                                            |                                                                                              
aTGATCAAACACACGACATAATAATAAATGATAAAAAAAATAAAATAGAAAATCATCTTGCACATGAATGTAGGCACCATAAAAATAAGAAGCTGAAgt                                                                                          >  2:836739/1‑99 (MQ=255)
 tGATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtg                                                                                         <  1:1055444/99‑1 (MQ=255)
  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:237190/99‑1 (MQ=255)
  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:643639/99‑1 (MQ=255)
  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:1918604/99‑1 (MQ=255)
  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:1894449/99‑1 (MQ=255)
  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:149444/99‑1 (MQ=255)
  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATAATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        >  2:3068131/1‑99 (MQ=255)
   aTTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGtgtg                                                                                       <  1:2612896/99‑1 (MQ=255)
    ttATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAACAATAAGAAGGTGAAGTGTGa                                                                                      >  2:2439678/1‑99 (MQ=255)
    ttATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGa                                                                                      >  2:1782103/1‑99 (MQ=255)
      atacgtacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATAATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAAt                                                                                    <  1:3120505/99‑1 (MQ=255)
      atacatacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTACACCTCTATTTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAAt                                                                                    >  2:2307635/1‑99 (MQ=255)
       tacatacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATa                                                                                   >  2:853438/1‑99 (MQ=255)
        acatacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATAc                                                                                  <  1:1762002/99‑1 (MQ=255)
          atacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCa                                                                                <  1:1378531/99‑1 (MQ=255)
          atacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCa                                                                                >  2:2622549/1‑99 (MQ=255)
          atacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCa                                                                                >  2:2049284/1‑99 (MQ=255)
          atacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCa                                                                                >  2:2041928/1‑99 (MQ=255)
           tacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAg                                                                               >  1:1020159/1‑99 (MQ=255)
           tacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAg                                                                               >  1:1574082/1‑99 (MQ=255)
            acGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGc                                                                              <  1:2430145/99‑1 (MQ=255)
            acGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACAAGc                                                                              >  2:1120848/1‑99 (MQ=255)
              gATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCaa                                                                            <  1:543125/99‑1 (MQ=255)
              gATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCaa                                                                            >  1:520819/1‑99 (MQ=255)
              gATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGAACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCaa                                                                            >  1:3114723/1‑99 (MQ=255)
                tataATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAat                                                                          >  1:1513137/1‑99 (MQ=255)
                 ataataataAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAata                                                                         >  1:1131245/1‑99 (MQ=255)
                   aataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATc                                                                       >  2:216713/1‑99 (MQ=255)
                   aataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATc                                                                       >  1:3176029/1‑99 (MQ=255)
                   aataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATc                                                                       >  1:2181378/1‑99 (MQ=255)
                   aataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGAAAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATc                                                                       <  1:3039204/99‑1 (MQ=255)
                    ataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCg                                                                      >  1:1358772/1‑99 (MQ=255)
                    ataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCg                                                                      >  2:2651562/1‑99 (MQ=255)
                     taataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACTTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCgg                                                                     >  1:617163/1‑99 (MQ=255)
                      aataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGACGTGTGAATACCAGCAAATATCGGa                                                                    <  1:3112868/99‑1 (MQ=255)
                       ataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGAt                                                                   >  1:2856419/1‑99 (MQ=255)
                        taaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGAtt                                                                  >  1:3051321/1‑99 (MQ=255)
                          aaTGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTa                                                                >  1:1679923/1‑99 (MQ=255)
                           aTGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTaa                                                               >  2:1519551/1‑99 (MQ=255)
                            tGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAAc                                                              <  1:1896363/99‑1 (MQ=255)
                               tAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACaaa                                                           >  2:623216/1‑99 (MQ=255)
                                 aaaaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATa                                                         >  2:792739/1‑99 (MQ=255)
                                  aaaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATAc                                                        <  1:1017565/99‑1 (MQ=255)
                                  aaaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATAc                                                        >  1:1249347/1‑99 (MQ=255)
                                   aaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACg                                                       >  1:1092792/1‑99 (MQ=255)
                                   aaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGATGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACg                                                       >  1:2693570/1‑99 (MQ=255)
                                      tataAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGtat                                                    <  1:1964351/99‑1 (MQ=255)
                                       ataAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGtata                                                   >  1:1233047/1‑99 (MQ=255)
                                         aaaaTTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAAc                                                 <  1:2924008/99‑1 (MQ=255)
                                           aaTTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGCAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAAAAAATACGTCTAatat                                               >  1:1735723/1‑99 (MQ=255)
                                           aaTTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACACGCAAATATCGGATTTAACAAATACGTATAACAt                                               >  2:1266918/1‑99 (MQ=255)
                                             ttGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTa                                             >  2:3148287/1‑99 (MQ=255)
                                             ttGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTa                                             >  2:2463695/1‑99 (MQ=255)
                                             ttGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTa                                             >  2:491186/1‑99 (MQ=255)
                                               gAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAAt                                           >  1:3003998/1‑99 (MQ=255)
                                                aaaaTCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATg                                          >  2:831872/1‑99 (MQ=255)
                                                    tCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCaaa                                      <  1:1588787/99‑1 (MQ=255)
                                                    tCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCaaa                                      <  1:954208/99‑1 (MQ=255)
                                                     cATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAAt                                     <  1:1690711/99‑1 (MQ=255)
                                                      aTGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAAtt                                    <  1:2290066/99‑1 (MQ=255)
                                                        gTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGTCTGTAACAAATACGTAAAACATTAATGCAAATTag                                  >  2:1487577/1‑98 (MQ=255)
                                                        gTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTAc                                  <  1:1206223/99‑1 (MQ=255)
                                                          tGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACtt                                <  1:2130253/99‑1 (MQ=255)
                                                          tGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACtt                                >  2:1815485/1‑99 (MQ=255)
                                                           gCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTa                               <  1:1836172/99‑1 (MQ=255)
                                                            cACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTaa                              <  1:2918088/99‑1 (MQ=255)
                                                            cACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTaa                              <  1:1642114/99‑1 (MQ=255)
                                                             aCCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAAc                             <  1:2072208/99‑1 (MQ=255)
                                                             aCCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAAc                             <  1:479731/99‑1 (MQ=255)
                                                             aCCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAAc                             <  1:478690/99‑1 (MQ=255)
                                                             aCCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAAc                             >  2:2785677/1‑99 (MQ=255)
                                                              ccTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACg                            <  1:2490594/99‑1 (MQ=255)
                                                              ccTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACg                            >  1:1057264/1‑99 (MQ=255)
                                                               ctctATCTAGGCTTGATAAAAATAAGAAGGTGAGGGGTGAATACGAGCAAATATCGGATTTAACAAATACGTATGACATTAATGCAAATTACTTAACgg                           >  2:1597625/1‑99 (MQ=255)
                                                               ctctATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACgg                           >  1:76587/1‑99 (MQ=255)
                                                                  tatctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtt                        <  1:3443854/99‑1 (MQ=255)
                                                                  tatctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtt                        >  2:2294096/1‑99 (MQ=255)
                                                                  tatctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtt                        <  1:1772962/99‑1 (MQ=255)
                                                                   atctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGttt                       >  1:3354790/1‑99 (MQ=255)
                                                                    tctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtttt                      <  1:3143948/99‑1 (MQ=255)
                                                                    tctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGCATTTAACAAATACGTATAACATTGATGCAAATTATTTACCGGGtttt                      >  2:1902470/1‑99 (MQ=255)
                                                                     ctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATGTAACAAATACGTATAACATTAATGCAACTTACTTAACGGGtgttt                     >  2:2622087/1‑99 (MQ=255)
                                                                      taGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtttttt                    >  1:1814016/1‑99 (MQ=255)
                                                                       atgCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACACTAATGCAAATTACTTAACGTGTTTTTTa                   <  2:2819626/97‑1 (MQ=255)
                                                                       aGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTa                   >  1:89920/1‑99 (MQ=255)
                                                                       aGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTa                   >  1:1485626/1‑99 (MQ=255)
                                                                       aGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTa                   <  1:1793456/99‑1 (MQ=255)
                                                                        ggCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTAc                  >  1:3032685/1‑99 (MQ=255)
                                                                         gCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACt                 >  2:853731/1‑99 (MQ=255)
                                                                         gCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAAAATTAATGCAAATTACTTAACGGGTTTTTTACt                 >  2:1385905/1‑99 (MQ=255)
                                                                          cTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCTGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACtt                <  1:1638971/99‑1 (MQ=255)
                                                                            tGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGt              >  2:104229/1‑99 (MQ=255)
                                                                               tAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCt           <  1:1921934/99‑1 (MQ=255)
                                                                                aaaaaTAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTa          >  1:1764245/1‑99 (MQ=255)
                                                                                  aaaTAAGAAGATGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTaaa        >  2:356904/1‑99 (MQ=255)
                                                                                   aaTAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTaaaa       >  1:2344845/1‑99 (MQ=255)
                                                                                     tAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAAcc     >  2:1661217/1‑99 (MQ=255)
                                                                                        gaaggTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAACCAga  <  2:3123141/99‑1 (MQ=255)
                                                                                        gaaggTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAACCAga  <  1:2010006/99‑1 (MQ=255)
                                                                                            |                                                                                              
ATGATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGATGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAACCAGA  >  NC_000913/2774378‑2774564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: