Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA29.4% 177.8 / 18.2 68A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (44/4);  new base A (0/20);  total (44/24)
Fisher's exact test for biased strand distribution p-value = 1.29e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATA  >  NC_000913/3665466‑3665922
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                              
tctTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCa                                                                                                                                                                                                                               <  2:205523/238‑1 (MQ=255)
   tttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCaaaa                                                                                                                                                                                                                            >  1:174418/1‑238 (MQ=255)
            cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCa                                                                                                                                                                                                                  >  1:1723640/1‑238 (MQ=255)
                      tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCa                                                                                                                                                                                                                               >  2:570541/1‑216 (MQ=255)
                      tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCa                                                                                                                                                                                                                               <  1:570541/216‑1 (MQ=255)
                                 ttaagtgtGCGATTGTGCCACAACTGCATAAAAACAAGTAATATCTCTCAGACGGTAATAAACGAG‑AAAGAAAAAATTTTTTTAATGT‑GA‑ATAAAGTTTTAA‑TG‑CCCCCTCCTTGAGCAG‑GGGGAAAAATAATGAAGGCGTG‑CCCCTAGACACAAAAAAAAAATTTTC‑AACCCCCTTA‑TCTACAAAATTT‑TGCCTTGCATCCGAAAAAACC‑GGGCAC‑CCGCCATTAAAATAGCGATAtt                                                                                                                                                                                           <  2:1537716/232‑1 (MQ=255)
                                        tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTaaaa                                                                                                                                                                                                     >  1:1162307/1‑222 (MQ=255)
                                        tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                    >  1:311799/1‑239 (MQ=255)
                                        tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                    >  1:1300481/1‑239 (MQ=255)
                                          cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                                   >  1:644193/1‑238 (MQ=255)
                                          cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                                   >  1:372105/1‑238 (MQ=255)
                                                  gcgcAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGCTATTCACCATTAACCATGGTg                                                                                                                                                                          >  1:979881/1‑239 (MQ=255)
                                                                       gAATATCTCTACAAAGGTAATAAACGAGAAATGAAAAACTTTTTTT‑ATGA‑GA‑AAAAAATTTTAA‑TC‑CCTCCTCTTTAACAAT‑GGGAAAAAATAATGAAAGCGTC‑TCCCTTAACACAAATAAAAAATTTAA‑AACACCTTTA‑TCACCTAGATTT‑TGTTTTACCCCCAAAAAACCC‑AGGCCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                           <  2:299514/217‑1 (MQ=255)
                                                                         attgctctAATAAAGTTATGAACGAATAAAAAAAAAATGTGTTA‑ATGG‑AA‑AAGAA‑ATTTAACTC‑CCCCCTCTTTAAGCAT‑CGGAAAAAAAAATAAAAGCGTT‑CCCTTTAACACAAAAAAAAAATTTAC‑AACCCACTTA‑TCTACTCAATTT‑TGGTTTGCACCCAAAAAAACA‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                                   <  2:134550/235‑1 (MQ=255)
                                                                              tatataaaaGTGAAAAAAGAATAAAGAAAAATTTTTTTA‑ATGT‑GA‑ATAAATATTTAA‑TC‑CCTCCTCCTGAAGAAT‑GGGGAAAAATAATGAAAGCGTG‑CCCATTAACAAAAAAAAAAAATTACC‑AACCCACTTC‑TCTACTAAAATT‑TGTTTTGCCTCCAAAAAAACA‑GGTTCC‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                                   <  2:387160/226‑1 (MQ=255)
                                                                                tactacAGGGAAGAAGAATAAATAAAGATATTTTTTT‑ATGT‑GA‑ATGAATATTTAA‑TG‑CCCCCTCTTGGAGCAT‑CGGAAAAAATAATAAAAGCGTG‑TCCCTTGACACAAAAAAAAAATTTCC‑AACACACTTA‑TCTACTAGAATT‑TGGTTTCCCCCCAAAAAAACC‑GGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                                      <  2:1300729/213‑1 (MQ=255)
                                                                                 actacAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATg                                                                                                                                                                             >  1:949577/1‑205 (MQ=255)
                                                                                 actacAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgagac                                                                                                                                                                      >  1:444460/1‑211 (MQ=255)
                                                                                 actacAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                                                                                 >  1:299514/1‑217 (MQ=255)
                                                                                   aagtataaataaCAAGAAATGAAGAATTTTTTTT‑ATGG‑AA‑AAAAAATTTTAA‑AC‑CCTCATTGTTAAGAAT‑GGGAAAAAATAATAAAGGTGTT‑CCCCTTAACAAGAAAAAAAAATTTAC‑ACAACACTTT‑TCTAATAAATTT‑TGTCTAAC‑CCCCAAAAAAAC‑AGGTTACCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                                         <  2:1028605/229‑1 (MQ=255)
                                                                                      agtaaggaACGAGTAAAGAAAAAATTTTTAT‑ATGT‑AC‑ATGAAGTTTTAA‑CG‑CCACCTCCTTGAGCAT‑CGGGAAAAAAAAAGAAAGCGTG‑TCCCTTGAAAAAAAAAAAAAATTTTC‑AACACATTTA‑TCTACTAGAATT‑TGGCTTCAACCCAAAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                      <  2:1511056/233‑1 (MQ=255)
                                                                                      aGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                      >  1:1933993/1‑239 (MQ=255)
                                                                                      aGTGAAGAACTAATAATGAAGAAATTTTTTA‑ATGT‑GC‑ATAAAGTTTTTA‑CT‑CCTCCACTTTGAGAAG‑GGGAAAAAAAAAAAAACGCGTC‑TCCCTTAACCATAATAAAAAATTTCC‑AACACTTTTA‑TCTACTAAATTT‑TGCCTTCCATCCACAAAACCC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                      <  2:469454/239‑1 (MQ=255)
                                                                                       ataaagaagaaGTAATAAAATAATTTTTTA‑ATGA‑GA‑AAGAAGTTTTAA‑GC‑CCTCCTTCTTAAACAT‑GGAAAAAAATAATAAAAGCGTG‑TCCCTTAACAAAAAAAAAAAATTTCC‑AACCCCCTTA‑TCTACAAGATTT‑TGGCTAGCACCCAAAAACACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                                                                                                           <  2:615968/223‑1 (MQ=255)
                                                                                        tgatgaACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                                                                                                >  1:1930789/1‑211 (MQ=255)
                                                                                        tgatgaACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                                         >  1:387160/1‑234 (MQ=255)
                                                                                        tgatgaACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACAAACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACA‑AGGTCA‑CCGCCATTAAAATAGCGATATTAACCATTAACCATgga                                                                                                                                                                           >  1:1216030/1‑199 (MQ=255)
                                                                                         gatgaAAGAGTAAGGAAGAATTTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TC‑CCTCCTCCTAGGGCAA‑CGAGAGAAAAAATGAAAGCTGA‑TCCTATGCAACAAATCAAAAATTTCA‑AACACACTTA‑TCTACTGAAATT‑GGGCATACACCCGAAAAACCG‑TGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAtt                                                                                                                                    <  2:1335788/238‑1 (MQ=255)
                                                                                          atgaACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                                                                                                            >  1:1300729/1‑213 (MQ=255)
                                                                                          atgaACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                   >  1:2265997/1‑238 (MQ=255)
                                                                                           tgaagaaatAATGAAAAAATTTTTTT‑ATGT‑AC‑ATAATGATTTAA‑TC‑CCCCCCCGTTAAACAT‑CGAGAAAAAAAATAAAAGCGTG‑CCCCTTACAAAAAATAAAAAATTTTC‑AACCCTCTTA‑TCTTCTAAATTT‑TGGTTTCCACCCAAAAAAACC‑GGTTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                                                                                                 <  2:644193/231‑1 (MQ=255)
                                                                                           gaaaCGAGTAA‑AAAAAAACTTTTTTAATTG‑GA‑AAGAAAATTAAA‑TG‑CCCCCTCTTTGAAAAA‑CGGGAAAAAAAATAAGCGCCTG‑TCCCTTGAAACAAATAAAAAATTTTA‑AAC‑TATTTATTCTACACAATTT‑TGTCTTCCTCCCAAAAAACCC‑GGGTAA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                                                                                                 <  2:311799/237‑1 (MQ=255)
                                                                                                 aGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                                                                                                 >  1:615968/1‑233 (MQ=255)
                                                                                                  gTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑ACGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatata                                                                                                                                                                  >  1:2056841/1‑199 (MQ=255)
                                                                                                  aagaataagaaACTTTTTT‑‑TGTGGCAATGAATATTTAA‑T‑‑CCTCCCCCTTTGGCATGAGGGAAAAATAAAG‑AACTGTGTTCTTTTTACAAAAAAAAAAAATTTCA‑ACCACCCTTT‑TCAACACAAATT‑TGTTTTACACCCAAAAAAACC‑AGGACA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                             <  2:1300481/231‑1 (MQ=255)
                                                                                                    aaTGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTa                                                                                                                            >  1:655188/1‑235 (MQ=255)
                                                                                                             tgtttttt‑ATGA‑GA‑ATAAATA‑TTAA‑TGCCCCCCTCCTGGAAAAA‑GGGAAAAAATAATGAAAGAGTG‑CCCTTTAAAAAAAAAAAAAAATTTTC‑ACCCCATTTT‑TCTAATCAATTT‑TGGCTTGAACCCAAAAAACCC‑GGGCAA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                               <  2:1723640/237‑1 (MQ=255)
                                                                                                                   ttatggt‑gA‑ATAAATATTTAA‑TG‑CCTCCACATGAACAAT‑GGGAAAAAATAAGAAAAGCTTT‑CCCCTTGACA‑AAAAAAAAAATTTTCAAACACATTTA‑CCATCACAATTT‑TGGTTTGCAACAAAAAAACCC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                          <  2:372105/234‑1 (MQ=255)
                                                                                                                                     tttAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTa                                                                                                                            >  1:1970874/1‑205 (MQ=255)
                                                                                                                                        taaagccctcctcctTGACCAG‑CGGAAAAAAAAAGAAATGCGTG‑CCCCTAGAAAAAAAAAAAAATTTAAC‑AACACTCTTA‑TCTACT‑GATTTGTGGTTTACACCCAAAAAAACC‑AGGCCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                                                                                         <  2:693885/153‑1 (MQ=255)
                                                                                                                                        aa‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                                                                                                     >  1:952624/1‑225 (MQ=255)
                                                                                                                                                tcctcctTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                                                                                 >  1:130937/1‑143 (MQ=255)
                                                                                                                                                  ctcctTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                                                                                                               >  1:693885/1‑159 (MQ=255)
                                                                                                                                                  ctcctTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TACCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                                                                                 >  1:1192305/1‑157 (MQ=255)
                                                                                                                                                    gctagcgCAT‑GGGAAAAAATAATGAAACGTTG‑CCCCTTGACATAAAAAAAAAATTCAC‑AACCCCCTTG‑TCTATTCAATTG‑TGGCTCACTCCAAAAAAAACG‑AGAACT‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                             <  2:312126/185‑1 (MQ=255)
                                                                                                                                                    cctTGAGCAG‑CGGGAAAAATAGTGAAAGCGTG‑TCCTTGGACAAAAAAAAAAAATTTCA‑AACACACTTA‑TCTACTGTAATT‑TGGTTTGCACCCAAAAAAACC‑GGTTAA‑CCGCCATTAAAATAGCGATATTCACCATTAACCa                                                                                                                                                                               <  2:653451/141‑1 (MQ=255)
                                                                                                                                                      ttGAGCAT‑GGGAAAAAAAAATAAATGCGTG‑TCCTTGAACAAGAAAAAAAAATTTCC‑ACCCCCCTTT‑TCTACTTAATTT‑TGTTTTCCCCCCGAAAAACCC‑AGGTCA‑CCGCCATTAAAATAGCg                                                                                                                                                                                                <  2:118915/122‑1 (MQ=255)
                                                                                                                                                               cGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCa                                                                                                                   >  1:312126/1‑191 (MQ=255)
                                                                                                                                                               cGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                                                                                                   >  1:208724/1‑207 (MQ=255)
                                                                                                                                                               cGGGAGAAATAATGAAAGCGTG‑TACCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                                                                                                     >  1:653451/1‑141 (MQ=255)
                                                                                                                                                                gggAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttatta                                                                           >  1:1186149/1‑230 (MQ=255)
                                                                                                                                                                 ggAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCa                                                                                                                                                                                      >  1:118915/1‑122 (MQ=255)
                                                                                                                                                                          atGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAg                                                                                                                >  1:1638641/1‑183 (MQ=255)
                                                                                                                                                                           tGAAAGCGTG‑TCCTTTAACAAAAAAAAAAAATTTAC‑AACCCTCTTT‑TCTACACGATTT‑TGGCTACCA‑CCAAAAAAACCGAGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                                         <  2:875912/157‑1 (MQ=255)
                                                                                                                                                                                gCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGAACGCCGCCCTCTTAAGTc                                                  >  2:1114925/1‑239 (MQ=255)
                                                                                                                                                                                      tCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                                                                                               >  1:875912/1‑157 (MQ=255)
                                                                                                                                                                                      tCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAatt                                             >  1:169943/1‑239 (MQ=255)
                                                                                                                                                                                           tGACACGAATACAAAATTTTC‑AAAACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                                                                                              >  1:2324188/1‑153 (MQ=255)
                                                                                                                                                                                                  aaTACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                                                                                                     >  1:1733803/1‑171 (MQ=255)
                                                                                                                                                                                                                      acTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGgag              >  1:980041/1‑239 (MQ=255)
                                                                                                                                                                                                                          a‑TCTACACGAATG‑TGGCTTGCACCAAAAAAAACG‑AGGTAA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                      <  2:290962/115‑1 (MQ=255)
                                                                                                                                                                                                                                 tCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAGATATTACTGTTTATTAATGTAGCACgccgcc                                                            >  1:365518/1‑183 (MQ=255)
                                                                                                                                                                                                                                 tCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGgatga    >  1:653722/1‑239 (MQ=255)
                                                                                                                                                                                                                                   gAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATa  >  1:1729321/1‑239 (MQ=255)
                                                                                                                                                                                                                                     aTT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTa                                                                                                                            >  1:290962/1‑115 (MQ=255)
                                                                                                                                                                                                                                            cTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                                      <  1:931510/67‑1 (MQ=255)
                                                                                                                                                                                                                                            cTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                                      >  2:931510/1‑67 (MQ=255)
                                                                                                                                                                                                                                                cATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                                                                                                            >  1:482121/1‑73 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                              
TCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑ATGT‑GC‑ATGAAGATTTAA‑TG‑CCTCCTCCTTGAGCAT‑CGGGAGAAATAATGAAAGCGTG‑TCCCTTGACACGAATACAAAATTTTC‑AACACACTTA‑TCTACTCGAATT‑TGGCTTGCATCCGCAAAAACC‑AGGTCA‑CCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATA  >  NC_000913/3665466‑3665922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑