Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,571,499 A→C L26V (TTG→GTG)  yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,571,4990AC100.0% 288.8 / NA 74L26V (TTG→GTG) yjiRputative DNA‑binding transcriptional regulator/putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (41/33);  total (41/33)

GCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATTGTGAACTGCCAGAAGGTGGATGATGAA  >  NC_000913/4571274‑4571724
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
gcgcCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCCCCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACCGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGa                                                                                                                                                                                                                      <  1:259004/239‑1 (MQ=255)
gcgcCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGa                                                                                                                                                                                                                      <  1:441406/239‑1 (MQ=255)
      ccATCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATAc                                                                                                                                                                                                                <  1:1149446/239‑1 (MQ=255)
      ccAGCATATCCAGCCCCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATAc                                                                                                                                                                                                                <  1:231756/239‑1 (MQ=255)
      ccAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCCCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATAc                                                                                                                                                                                                                <  1:388522/239‑1 (MQ=255)
           atatCCAGCCCCTGATCCAACTGGGTAATTTCCACCGGGCGCTGCCCCGGACGCGTCATCGGCGGTACGGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGcc                                                                                                                                                                                                            <  2:710424/238‑1 (MQ=255)
                                         tCCACCGGGCGGTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGc                                                                                                                                                                             <  1:958559/239‑1 (MQ=255)
                                                     tGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGTTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATa                                                                                                                                                                 <  1:1156042/239‑1 (MQ=255)
                                                     tGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGAt                                                                                                                                                                  <  1:295645/238‑1 (MQ=255)
                                                       cACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGccc                                                                                                                                                                                                           <  1:967485/195‑1 (MQ=255)
                                                       cACCGGACGCGTCATCGGCGGGACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAc                                                                                                                                                               <  1:587533/239‑1 (MQ=255)
                                                              cgcgTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCa                                                                                                                                                         <  1:1131188/238‑1 (MQ=255)
                                                                       ggcggTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTc                                                                                                                                               <  1:173197/239‑1 (MQ=255)
                                                                                  cggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGa                                                                                                                                     <  1:20142/238‑1 (MQ=255)
                                                                                              aCGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAgagaga                                                                                                                        <  1:1112454/239‑1 (MQ=255)
                                                                                                   gtgCGACCAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGGCGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAAc                                                                                                                   <  1:1175852/239‑1 (MQ=255)
                                                                                                    tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAAcc                                                                                                                  >  2:729618/1‑239 (MQ=255)
                                                                                                            aaTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGa                                                                                                          <  1:27902/239‑1 (MQ=255)
                                                                                                              tAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCg                                                                                                                                                                                                                         <  2:890308/126‑1 (MQ=255)
                                                                                                              tAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCg                                                                                                                                                                                                                         >  1:890308/1‑126 (MQ=255)
                                                                                                                 cccGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCagag                                                                                                                                                                                 >  1:475730/1‑163 (MQ=255)
                                                                                                                 cccGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCagag                                                                                                                                                                                 <  2:475730/163‑1 (MQ=255)
                                                                                                                     aaCGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTaa                                                                                                 >  2:363812/1‑239 (MQ=255)
                                                                                                                          ggCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAtgt                                                                                                                                        <  1:242232/195‑1 (MQ=255)
                                                                                                                          ggCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAtgt                                                                                                                                        >  2:242232/1‑195 (MQ=255)
                                                                                                                            cTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGcc                                                                                                                                                                                                            <  2:475473/125‑1 (MQ=255)
                                                                                                                            cTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGcc                                                                                                                                                                                                            >  1:475473/1‑125 (MQ=255)
                                                                                                                            cTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt                                                                                                                                          <  2:1059607/191‑1 (MQ=255)
                                                                                                                            cTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt                                                                                                                                          >  1:1059607/1‑191 (MQ=255)
                                                                                                                               cGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCg                                                                                                                                                                                           <  2:775638/139‑1 (MQ=255)
                                                                                                                               cGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCg                                                                                                                                                                                           >  1:775638/1‑139 (MQ=255)
                                                                                                                                              cATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGCGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGAt                                                                                 >  1:1049193/1‑230 (MQ=255)
                                                                                                                                              cATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTa                                                                        >  2:656438/1‑239 (MQ=255)
                                                                                                                                                                      ctgctgCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAcgcg                                                                                                                                                            <  1:915087/131‑1 (MQ=255)
                                                                                                                                                                      ctgctgCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAcgcg                                                                                                                                                            >  2:915087/1‑131 (MQ=255)
                                                                                                                                                                          tgcACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAat                                            >  1:10904/1‑239 (MQ=255)
                                                                                                                                                                                gTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATa                                                                                                                                                                                                                 <  1:371555/68‑1 (MQ=255)
                                                                                                                                                                                gTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATa                                                                                                                                                                                                                 >  2:371555/1‑68 (MQ=255)
                                                                                                                                                                                   cTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAAc                                                                      >  1:989235/1‑204 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTg                                                                                                                                                                                                        <  2:511860/66‑1 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTg                                                                                                                                                                                                        >  1:511860/1‑66 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGc                                                                                                                                                      <  1:830064/116‑1 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGc                                                                                                                                                      >  2:830064/1‑116 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACa                                                                                                                              <  1:292125/140‑1 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACa                                                                                                                              >  2:292125/1‑140 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAAc                                                                      >  1:459360/1‑196 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATg                                                           >  1:34743/1‑207 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTAtt                             >  1:668131/1‑237 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATtgt                           >  2:95547/1‑239 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATtgt                           >  2:810789/1‑239 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATtgt                           >  1:1027508/1‑239 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATtgt                           >  1:142067/1‑239 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATtgt                           >  1:278499/1‑239 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATtgt                           >  1:545764/1‑239 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATtgt                           >  1:653689/1‑239 (MQ=255)
                                                                                                                                                                                           gACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATtgt                           >  1:602132/1‑239 (MQ=255)
                                                                                                                                                                                             cGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTgg                                                                                                                                      >  1:527405/1‑130 (MQ=255)
                                                                                                                                                                                             cGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTgg                                                                                                                                      <  2:527405/130‑1 (MQ=255)
                                                                                                                                                                                                cGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAgagaga                                                                                                                        <  1:758187/141‑1 (MQ=255)
                                                                                                                                                                                                cGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAgagaga                                                                                                                        >  2:758187/1‑141 (MQ=255)
                                                                                                                                                                                                cGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAAc                                                                                                                   >  1:78229/1‑146 (MQ=255)
                                                                                                                                                                                                cGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAAc                                                                                                                   <  2:78229/146‑1 (MQ=255)
                                                                                                                                                                                                  tGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATTGTGa                         >  2:1099794/1‑234 (MQ=255)
                                                                                                                                                                                                  tGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATTGTGa                         >  2:679398/1‑234 (MQ=255)
                                                                                                                                                                                                            ttAAGCTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACct                                                                    >  1:799915/1‑181 (MQ=255)
                                                                                                                                                                                                                 cTGCGCACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATTGTGAACTGCCAGAAg              >  1:983863/1‑230 (MQ=255)
                                                                                                                                                                                                                    cgcACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATTGTGAACTGCCAGAAGGTGga         >  1:852852/1‑232 (MQ=255)
                                                                                                                                                                                                                    cgcACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATTGTGAACTGCCAGAAGGTGGATGATGaa  >  2:485541/1‑239 (MQ=255)
                                                                                                                                                                                                                    cgcACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATTGTGAACTGCCAGAAGGTGGATGATGaa  >  1:905313/1‑239 (MQ=255)
                                                                                                                                                                                                                    cgcACCGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATTGTGAACTGCCAGAAGGTGGATGATGaa  >  1:1047636/1‑239 (MQ=255)
                                                                                                                                                                                                                         cGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAtgt                                                                                                                                        <  1:547463/100‑1 (MQ=255)
                                                                                                                                                                                                                         cGACGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAtgt                                                                                                                                        >  2:547463/1‑100 (MQ=255)
                                                                                                                                                                                                                           aCGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGc                                                                                                                                                                             <  1:571319/61‑1 (MQ=255)
                                                                                                                                                                                                                           aCGGCACTTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGc                                                                                                                                                                             >  2:571319/1‑61 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
GCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTGGACCATACAGAGAGAAAAACCGGTACAGATGAGGCTAAAAAACCGGATGCAGATGCTGTTTTAACCTGTCTGTATGTTAAATAAAAGTAATATTTGAATCTGTATTGTGAACTGCCAGAAGGTGGATGATGAA  >  NC_000913/4571274‑4571724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: