Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,572,080 T→G intergenic (+165/‑334) yjiS → / → yjiT DUF1127 family protein/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,572,0800TG100.0% 338.7 / NA 89intergenic (+165/‑334)yjiS/yjiTDUF1127 family protein/pseudogene
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (50/39);  total (50/39)

GTTACAGCAGATGAGTGATGAGCGGTTGAAGGATATCGGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAAT  >  NC_000913/4571852‑4572307
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   
gtTACAGCAGATGAGTGATGAGCGGTTGAAGGATATCGGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGtt                                                                                                                                                                                                                            <  1:257034/238‑1 (MQ=255)
           tGAGTGATGAGCGGTTGAAGGATATCGGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTtgact                                                                                                                                                                                                                    <  2:886823/235‑1 (MQ=255)
                                 tatCGGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTa                                                                                                                                                                                          <  2:478291/239‑1 (MQ=255)
                                                     aTGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTa                                                                                                                                                                          >  1:682329/1‑235 (MQ=255)
                                                        tgGAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGttt                                                                                                                                                                   >  1:1160051/1‑239 (MQ=255)
                                                         ggAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                                                                                                                  <  2:682329/239‑1 (MQ=255)
                                                               aGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCattatt                                                                                                                                                                                               >  2:514956/1‑204 (MQ=255)
                                                               aGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCattatt                                                                                                                                                                                               <  1:514956/204‑1 (MQ=255)
                                                               aGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTAGATAACGGTCAAAAAACGCACAGTGATGACTTGAGTTTTTTGACTGACTTTACAACTATCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATAc                                                                                                                                                              >  2:458908/1‑237 (MQ=255)
                                                                  ggggATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGATCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCattatt                                                                                                                                                                                               >  2:868590/1‑201 (MQ=255)
                                                                  ggggATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGATCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCattatt                                                                                                                                                                                               <  1:868590/201‑1 (MQ=255)
                                                                              ttATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                                                                                                         >  1:240881/1‑211 (MQ=255)
                                                                              ttATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                                                                                                         <  2:240881/211‑1 (MQ=255)
                                                                                atatataATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                                                                                                                  >  1:1136582/1‑216 (MQ=255)
                                                                                atatataATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                                                                                                                  <  2:1136582/216‑1 (MQ=255)
                                                                                      aataaAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCt                                                                                                                                        >  1:106413/1‑236 (MQ=255)
                                                                                             cGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGa                                                                                                                                                        <  2:968561/213‑1 (MQ=255)
                                                                                             cGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGa                                                                                                                                                        >  1:968561/1‑213 (MQ=255)
                                                                                                        ttttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCa                                                                                                                                                                                   >  2:645841/1‑175 (MQ=255)
                                                                                                        ttttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCa                                                                                                                                                                                   <  1:645841/175‑1 (MQ=255)
                                                                                                        ttttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGtt                                                                                                                   >  1:997975/1‑239 (MQ=255)
                                                                                                         tttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTa                                                                                                                                       >  2:903505/1‑218 (MQ=255)
                                                                                                         tttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTa                                                                                                                                       <  1:903505/218‑1 (MQ=255)
                                                                                                                                       aCAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                                                                                                         >  1:247584/1‑154 (MQ=255)
                                                                                                                                       aCAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACGGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                                                                                                         <  2:247584/154‑1 (MQ=255)
                                                                                                                                            tcatcaATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGaa                                                                                                                                  >  1:415444/1‑188 (MQ=255)
                                                                                                                                            tcatcaATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGaa                                                                                                                                  <  2:415444/188‑1 (MQ=255)
                                                                                                                                                caATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATa                                                                           >  1:491414/1‑239 (MQ=255)
                                                                                                                                                      gTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACa                                                                                                                                                                                                           >  1:609582/1‑105 (MQ=255)
                                                                                                                                                      gTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACa                                                                                                                                                                                                           <  2:609582/105‑1 (MQ=255)
                                                                                                                                                       tATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAg                                                                     <  2:1160051/238‑1 (MQ=255)
                                                                                                                                                              tgtACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                                                                                                                  <  2:819468/138‑1 (MQ=255)
                                                                                                                                                              tgtACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                                                                                                                  >  1:819468/1‑138 (MQ=255)
                                                                                                                                                               gtACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGAt                                                              <  2:491414/237‑1 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTa                                                                                                                                                                                          >  2:588945/1‑112 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTa                                                                                                                                                                                          <  1:588945/112‑1 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGaa                                                                                                                                  >  1:115562/1‑168 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGaa                                                                                                                                  <  2:115562/168‑1 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAg                                                                     <  2:258698/229‑1 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAg                                                                     >  1:258698/1‑229 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGtt                                                                   <  2:44864/231‑1 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGtt                                                                   >  1:44864/1‑231 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTaa                                                           >  1:600221/1‑239 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTaa                                                           >  2:782629/1‑239 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTaa                                                           >  2:624869/1‑239 (MQ=255)
                                                                                                                                                                tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTaa                                                           >  2:903972/1‑239 (MQ=255)
                                                                                                                                                                     gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGa                                                                                                                                                        <  2:271327/141‑1 (MQ=255)
                                                                                                                                                                     gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGa                                                                                                                                                        >  1:271327/1‑141 (MQ=255)
                                                                                                                                                                     gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTTAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGAt                                                      >  1:1080138/1‑239 (MQ=255)
                                                                                                                                                                     gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGtt                                                                   >  1:442005/1‑226 (MQ=255)
                                                                                                                                                                     gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGtt                                                                   <  2:442005/226‑1 (MQ=255)
                                                                                                                                                                     gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGAt                                                      >  2:1145089/1‑239 (MQ=255)
                                                                                                                                                                        tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAAt                                                                                    >  1:537106/1‑206 (MQ=255)
                                                                                                                                                                        tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAAt                                                                                    <  2:537106/206‑1 (MQ=255)
                                                                                                                                                                        tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGt                                                   <  2:600221/239‑1 (MQ=255)
                                                                                                                                                                        tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGt                                                   >  1:701309/1‑239 (MQ=255)
                                                                                                                                                                        tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGt                                                   >  2:946181/1‑239 (MQ=255)
                                                                                                                                                                          tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGttt                                                                                                                  >  2:1130475/1‑174 (MQ=255)
                                                                                                                                                                          tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGttt                                                                                                                  <  1:1130475/174‑1 (MQ=255)
                                                                                                                                                                          tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGat                                                                                                             >  2:151902/1‑179 (MQ=255)
                                                                                                                                                                          tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGat                                                                                                             <  1:151902/179‑1 (MQ=255)
                                                                                                                                                                          tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGtt                                                                   >  1:583958/1‑221 (MQ=255)
                                                                                                                                                                          tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGtt                                                                   <  2:583958/221‑1 (MQ=255)
                                                                                                                                                                               ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTAc                                                                                                                                                                                                            >  2:930716/1‑79 (MQ=255)
                                                                                                                                                                               ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTAc                                                                                                                                                                                                            <  1:930716/79‑1 (MQ=255)
                                                                                                                                                                               ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAAt                                                                                                                                               <  2:671413/140‑1 (MQ=255)
                                                                                                                                                                               ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAAt                                                                                                                                               >  1:671413/1‑140 (MQ=255)
                                                                                                                                                                               ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGTTGATATc                                            >  1:1073903/1‑239 (MQ=255)
                                                                                                                                                                                      aCCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTa                                     >  1:773486/1‑239 (MQ=255)
                                                                                                                                                                                      aCCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGAGATTAATAGATGGTTGATATCATTTTTa                                     >  2:76804/1‑239 (MQ=255)
                                                                                                                                                                                         tctTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATtgtg                                                                                                                                                                                     >  2:520466/1‑92 (MQ=255)
                                                                                                                                                                                         tctTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATtgtg                                                                                                                                                                                     <  1:520466/92‑1 (MQ=255)
                                                                                                                                                                                           tttAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAgg                                                                                <  2:491344/191‑1 (MQ=255)
                                                                                                                                                                                           tttAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAgg                                                                                >  1:491344/1‑191 (MQ=255)
                                                                                                                                                                                           tttAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGAt                                                              >  2:203524/1‑209 (MQ=255)
                                                                                                                                                                                           tttAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGAt                                                              <  1:203524/209‑1 (MQ=255)
                                                                                                                                                                                             tAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTaaaa                              <  2:997975/239‑1 (MQ=255)
                                                                                                                                                                                                    aTGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGt                                                                               <  1:412497/183‑1 (MQ=255)
                                                                                                                                                                                                    aTGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGt                                                                               >  2:412497/1‑183 (MQ=255)
                                                                                                                                                                                                     tGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGGCTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAAt                                                                                    <  1:966156/177‑1 (MQ=255)
                                                                                                                                                                                                     tGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGGCTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAAt                                                                                    >  2:966156/1‑177 (MQ=255)
                                                                                                                                                                                                     tGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                                                                                                         <  1:898527/92‑1 (MQ=255)
                                                                                                                                                                                                     tGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                                                                                                         >  2:898527/1‑92 (MQ=255)
                                                                                                                                                                                                     tGTCGATAACGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTta                      >  2:874713/1‑239 (MQ=255)
                                                                                                                                                                                                              cGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                                                                                                         >  1:875944/1‑83 (MQ=255)
                                                                                                                                                                                                              cGGTCAAAAAACGCACAGTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                                                                                                         <  2:875944/83‑1 (MQ=255)
                                                                                                                                                                                                                         cGCACAGCGATGACTTGTGTTTTTTGACTGACTTTACAACTTTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAAt  >  1:403505/1‑239 (MQ=255)
                                                                                                                                                                                                                               gTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGAt                                                              >  2:162104/1‑173 (MQ=255)
                                                                                                                                                                                                                               gTGATGACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGAt                                                              <  1:162104/173‑1 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   
GTTACAGCAGATGAGTGATGAGCGGTTGAAGGATATCGGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAAT  >  NC_000913/4571852‑4572307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: