Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 682,878 A→C D172E (GAT→GAG)  hscC ← Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913682,8780AC100.0% 264.0 / NA 73D172E (GAT→GAG) hscCHsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/38);  total (35/38)

TGCAATGGCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGA  >  NC_000913/682648‑683111
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
tgCAATGGCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAaca                                                                                                                                                                                                                                    >  2:619235/1‑238 (MQ=255)
           ttgattgCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACgg                                                                                                                                                                                                                         <  1:625809/238‑1 (MQ=255)
           ttgattgCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACgg                                                                                                                                                                                                                         <  1:241618/238‑1 (MQ=255)
            tgattgCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGt                                                                                                                                                                                                                       <  1:912497/239‑1 (MQ=255)
                     cATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACCCCAGCGAACGGGGATTttgttg                                                                                                                                                                                                              >  2:841243/1‑239 (MQ=255)
                             cGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCa                                                                                                                                                                                                      <  1:102290/239‑1 (MQ=255)
                             cGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCa                                                                                                                                                                                                      <  1:21021/239‑1 (MQ=255)
                                          gCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTttgttg                                                                                                                                                                                                              <  1:679239/218‑1 (MQ=255)
                                          gCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTttgttg                                                                                                                                                                                                              >  2:679239/1‑218 (MQ=255)
                                           cGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGAGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATc                                                                                                                                                                                        >  2:738963/1‑239 (MQ=255)
                                                     tGCCAGTTCACTCTCGGTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAgctgct                                                                                                                                                                               <  1:599293/238‑1 (MQ=255)
                                                                tctcGTTAAGCTTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCatta                                                                                                                                                                   <  2:866618/239‑1 (MQ=255)
                                                                  tcGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCa                                                                                                                                                                                                      <  2:649145/202‑1 (MQ=255)
                                                                  tcGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGGCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCa                                                                                                                                                                                                      >  1:649145/1‑202 (MQ=255)
                                                                     ttAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCAttaattaa                                                                                                                                                              >  2:1166004/1‑239 (MQ=255)
                                                                      acaGCGTGGTCCTGACGACATCCGCGCGTGTTAAACCCTCATCGACCAGCAGATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCTCCGTATCGTCAAACGTGCCGCCCCCGAGCACAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCAttaattaa                                                                                                                                                              <  2:1154119/236‑1 (MQ=255)
                                                                          cGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATg                                                                                                                                                                                                        >  2:554975/1‑192 (MQ=255)
                                                                          cGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATg                                                                                                                                                                                                        <  1:554975/192‑1 (MQ=255)
                                                                                    gCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCCCCAGCGCAGGCGTGAACTTCCATAACCGGCGTGGCGTAATAAAGCACCGTAACGTAAAACGTGCCGCCACCGGGCTCAAACACCACCGACCGGGGATTTTGTTGGGTATGCAGGCCCTTCGCCCTCGCAGCAGCTTTGGGTTCCTTAATTTAGCGttctctcattg                                                                                                                                                >  2:1104086/1‑228 (MQ=255)
                                                                                         aTCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGctgctg                                                                                                                                                                              >  2:69574/1‑203 (MQ=255)
                                                                                         aTCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGctgctg                                                                                                                                                                              <  1:69574/203‑1 (MQ=255)
                                                                                            cgcgcgTTTTAAAACCTCATCTACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTa                                                                                                                                       <  1:792440/239‑1 (MQ=255)
                                                                                            cgcgcgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAAc                                                                                                                                              <  1:619235/232‑1 (MQ=255)
                                                                                              cgcgTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTa                                                                                                                                       >  2:895234/1‑237 (MQ=255)
                                                                                                                       cATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGc                                                                                                            >  1:193759/1‑239 (MQ=255)
                                                                                                                        atatGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGc                                                                                                                <  1:1166004/234‑1 (MQ=255)
                                                                                                                                 aaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAAc                                                                                                                                                                                                                           >  1:98827/1‑118 (MQ=255)
                                                                                                                                 aaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAAc                                                                                                                                                                                                                           <  2:98827/118‑1 (MQ=255)
                                                                                                                                 aaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTCTTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATc                                                                                                  >  1:478229/1‑239 (MQ=255)
                                                                                                                                 aaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTgg                                                                                                                                                                           <  2:32210/166‑1 (MQ=255)
                                                                                                                                 aaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTgg                                                                                                                                                                           >  1:32210/1‑166 (MQ=255)
                                                                                                                                 aaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATc                                                                                                  >  2:319722/1‑239 (MQ=255)
                                                                                                                                 aaaTCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGCATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACCACGCTGCTAAACGGGTATGCTTGCGTTTTTCATc                                                                                                  >  1:245004/1‑239 (MQ=255)
                                                                                                                                   aTCTTCGCCACCAATAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGGGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGc                                                                                                <  2:986130/239‑1 (MQ=255)
                                                                                                                                              caAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCatta                                                                                                                                                                   >  1:302046/1‑161 (MQ=255)
                                                                                                                                              caAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCatta                                                                                                                                                                   <  2:302046/161‑1 (MQ=255)
                                                                                                                                                       tGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGaa                                                                                 <  1:722833/234‑1 (MQ=255)
                                                                                                                                                                               cAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAGCGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGt                                                    <  1:375628/239‑1 (MQ=255)
                                                                                                                                                                               cAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAAc                                                                                                                                                                                                                           <  1:215556/72‑1 (MQ=255)
                                                                                                                                                                               cAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAAc                                                                                                                                                                                                                           >  2:215556/1‑72 (MQ=255)
                                                                                                                                                                                       ggcgtggcgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGaaa                                            >  2:664755/1‑239 (MQ=255)
                                                                                                                                                                                           tggcgtACTCAAGCACCGTAACGTCAAACGTGCCCCCACCGAGCTCAAACACCAGCGAACGGGTATTTTATTGGGTATGCAGGCCATACGCCATCGCCGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTCGCGTTGTGCATCGCTGAAATAAGCCGGAACGCAGATCACCACATCTTTAATCGGACTTTCCAGAATttct                                        >  2:546688/1‑239 (MQ=255)
                                                                                                                                                                                            ggcgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTggg                                                                                                                                                                          >  1:832976/1‑108 (MQ=255)
                                                                                                                                                                                            ggcgtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTggg                                                                                                                                                                          <  2:832976/108‑1 (MQ=255)
                                                                                                                                                                                               gtACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCgg                                    <  1:319722/239‑1 (MQ=255)
                                                                                                                                                                                                   tCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCCGCTGCTGTGGGTTCATTAATTAAGCGTAc                                                                                                                                                        >  1:1000584/1‑119 (MQ=255)
                                                                                                                                                                                                   tCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTAc                                                                                                                                                        <  2:1000584/119‑1 (MQ=255)
                                                                                                                                                                                                   tCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCAtc                                <  1:895234/239‑1 (MQ=255)
                                                                                                                                                                                                        cACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGc                                                                                      >  1:563347/1‑180 (MQ=255)
                                                                                                                                                                                                        cACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGc                                                                                      <  2:563347/180‑1 (MQ=255)
                                                                                                                                                                                                        cACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGAt                                                                          >  2:755435/1‑192 (MQ=255)
                                                                                                                                                                                                        cACCGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGAt                                                                          <  1:755435/192‑1 (MQ=255)
                                                                                                                                                                                                           cGTAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTa                        >  1:454900/1‑239 (MQ=255)
                                                                                                                                                                                                             tAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAAt                      >  1:707408/1‑239 (MQ=255)
                                                                                                                                                                                                             tAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAAt                      >  1:644325/1‑239 (MQ=255)
                                                                                                                                                                                                             tAACGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGGATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAAt                      >  1:338838/1‑239 (MQ=255)
                                                                                                                                                                                                                cGTCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGTCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAg                   <  1:576608/239‑1 (MQ=255)
                                                                                                                                                                                                                  tCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCg                 <  1:237991/239‑1 (MQ=255)
                                                                                                                                                                                                                  tCAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCg                 >  1:1093043/1‑239 (MQ=255)
                                                                                                                                                                                                                   cAAACGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCg                 <  1:664755/238‑1 (MQ=255)
                                                                                                                                                                                                                     aaCGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTAcc                                                                                                                                                       >  1:25764/1‑102 (MQ=255)
                                                                                                                                                                                                                     aaCGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTAcc                                                                                                                                                       <  2:25764/102‑1 (MQ=255)
                                                                                                                                                                                                                     aaCGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATcac                                                                       <  1:944772/182‑1 (MQ=255)
                                                                                                                                                                                                                     aaCGTGCCGCCACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATcac                                                                       >  2:944772/1‑182 (MQ=255)
                                                                                                                                                                                                                           ccgccACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAAc                                                                                                                                              >  1:711581/1‑105 (MQ=255)
                                                                                                                                                                                                                           ccgccACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAAc                                                                                                                                              <  2:711581/105‑1 (MQ=255)
                                                                                                                                                                                                                           ccgccACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCa                                                                                                    >  2:189740/1‑147 (MQ=255)
                                                                                                                                                                                                                           ccgccACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCa                                                                                                    <  1:189740/147‑1 (MQ=255)
                                                                                                                                                                                                                           ccgccACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGt                                                    >  1:638952/1‑195 (MQ=255)
                                                                                                                                                                                                                           ccgccACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGt                                                    <  2:638952/195‑1 (MQ=255)
                                                                                                                                                                                                                           ccgccACCGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCaa        >  2:380852/1‑239 (MQ=255)
                                                                                                                                                                                                                                 ccGAGCTCAAACCCCCGCGAACGGGTTTTTTGTTGGGTATGCAGGCCATACGCCATCGCCGGTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGa  <  1:681095/239‑1 (MQ=255)
                                                                                                                                                                                                                                 ccGAGCTCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGa  <  1:738963/239‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
TGCAATGGCGATTGATTGCTACATTTTGCCGCTTCCACACAGGCGTACAAGGCTGCCAGTTCACTCTCGTTAAGCGTGGTCCTGGCGACATCCGCGCGTTTTAAAACCTCATCGACCAGCATATGGGTAAAATCTTCGCCACCAAGAAAGTTGTCGCCAGCGGAGGCGTGAACTTCAATCACCGGCGTGGCGTACTCAAGCACCGTAACGTCAAACGTGCCGCCACCGAGATCAAACACCAGCGAACGGGTATTTTGTTGGGTATGCAGGCCATACGCCATCGCAGCTGCTGTGGGTTCATTAATTAAGCGTACCGCATTTAACCCGGCTAACTCCGCTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGA  >  NC_000913/682648‑683111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑