Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 691,280 T→G I169L (ATT→CTT)  ybeX ← putative ion transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913691,2800TG100.0% 281.2 / NA 75I169L (ATT→CTT) ybeXputative ion transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/35);  total (40/35)

GCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGAT  >  NC_000913/691048‑691502
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   
gCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGAt                                                                                                                                                                                                                               <  2:61864/239‑1 (MQ=255)
  cTGCATCACTAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGAt                                                                                                                                                                                                                               <  1:8245/237‑1 (MQ=255)
  cTGCATCACTAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGAt                                                                                                                                                                                                                               >  2:8245/1‑237 (MQ=255)
   tGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCg                                                                                                                                                                                                                             >  2:481915/1‑238 (MQ=255)
     cATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCa                                                                                                                                                                                                                          <  2:31325/239‑1 (MQ=255)
               ccaccGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAAc                                                                                                                                                                                                                 >  2:1163056/1‑238 (MQ=255)
                  ccGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAAc                                                                                                                                                                                                                 >  1:78470/1‑235 (MQ=255)
                       aGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGc                                                                                                                                                                                                        >  2:1023751/1‑239 (MQ=255)
                           tCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGACACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGATAGCGaa                                                                                                                                                                                                     >  1:306146/1‑238 (MQ=255)
                                  cttcATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTc                                                                                                                                                                                             <  2:484215/239‑1 (MQ=255)
                                   ttcATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTACAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGCCTTCATTCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGCCCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCGGAGAGCGAACCTCtttta                                                                                                                                                                                            >  2:126737/1‑237 (MQ=255)
                                        cGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCCCCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATc                                                                                                                                                                                       <  1:126737/239‑1 (MQ=255)
                                                     gCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGCCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCt                                                                                                                                                                          >  1:803036/1‑239 (MQ=255)
                                                            gCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCa                                                                                                                                                                                                                          >  1:534401/1‑184 (MQ=255)
                                                            gCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCa                                                                                                                                                                                                                          <  2:534401/184‑1 (MQ=255)
                                                              ttCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGaaa                                                                                                                                                                                                    <  2:839624/204‑1 (MQ=255)
                                                              ttCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGaaa                                                                                                                                                                                                    >  1:839624/1‑204 (MQ=255)
                                                                          ttCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AAcg                                                                                                                                                 >  2:392055/1‑237 (MQ=255)
                                                                          ttCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGCCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AAcc                                                                                                                                                 >  2:851752/1‑238 (MQ=255)
                                                                            aaaTGGAAGCCAGTGCGCGTACGGTCAAGGTATGACGACTCAGCTGACGGAAGTCGATTTCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAAACCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGcc                                                                                                                                              <  1:570722/238‑1 (MQ=255)
                                                                                                aCGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCa                                                                                                                           <  1:865205/238‑1 (MQ=255)
                                                                                                   gTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTga                                                                                                                                                                                                            >  2:1076755/1‑159 (MQ=255)
                                                                                                   gTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTga                                                                                                                                                                                                            <  1:1076755/159‑1 (MQ=255)
                                                                                                              acgacTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTga                                                                                                                                                                                                            <  1:1149432/148‑1 (MQ=255)
                                                                                                              acgacTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTga                                                                                                                                                                                                            >  2:1149432/1‑148 (MQ=255)
                                                                                                              acgacTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCa                                                                                                             >  2:716303/1‑238 (MQ=255)
                                                                                                              acgacTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCa                                                                                                             >  1:99246/1‑238 (MQ=255)
                                                                                                              acgacTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCa                                                                                                             >  2:6720/1‑238 (MQ=255)
                                                                                                              acgacTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCa                                                                                                             >  2:694663/1‑238 (MQ=255)
                                                                                                              acgacTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACAGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAg                                                                                                            >  2:74145/1‑239 (MQ=255)
                                                                                                                  cTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGcc                                                                                                                                              <  1:131359/201‑1 (MQ=255)
                                                                                                                  cTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGcc                                                                                                                                              >  2:131359/1‑201 (MQ=255)
                                                                                                                     aGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑Ac                                                                                                                                                        <  1:188429/191‑1 (MQ=255)
                                                                                                                     aGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑Ac                                                                                                                                                        >  2:188429/1‑191 (MQ=255)
                                                                                                                        tGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGc                                                                                                      <  2:78470/235‑1 (MQ=255)
                                                                                                                                 tCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACg                                                                                          >  2:290481/1‑238 (MQ=255)
                                                                                                                                          tcttctTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTg                                                                                                                                            <  2:1042010/179‑1 (MQ=255)
                                                                                                                                          tcttctTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTg                                                                                                                                            >  1:1042010/1‑179 (MQ=255)
                                                                                                                                           cttcttCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCt                                                                               >  1:867071/1‑239 (MQ=255)
                                                                                                                                                 cGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTc                                                                                                                             >  1:508024/1‑187 (MQ=255)
                                                                                                                                                 cGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTc                                                                                                                             <  2:508024/187‑1 (MQ=255)
                                                                                                                                                             cTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCt                                                             <  1:481915/239‑1 (MQ=255)
                                                                                                                                                                cAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGGAACGATAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAACCGGCAGGAAGTCTTTCGCCATCAGAATc                                                                >  2:525833/1‑238 (MQ=255)
                                                                                                                                                                 aaTCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑a                                                                                                                                                    <  1:1175619/148‑1 (MQ=255)
                                                                                                                                                                 aaTCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑a                                                                                                                                                    >  2:1175619/1‑148 (MQ=255)
                                                                                                                                                                   tctcACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGTTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAtg                                                       <  2:803036/239‑1 (MQ=255)
                                                                                                                                                                   tctcACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAtg                                                       <  2:768386/239‑1 (MQ=255)
                                                                                                                                                                     tcACCGACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAtgtg                                                     <  1:968553/239‑1 (MQ=255)
                                                                                                                                                                       aCCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATc                                                                                                                                                                                       <  2:34765/112‑1 (MQ=255)
                                                                                                                                                                       aCCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATc                                                                                                                                                                                       >  1:34765/1‑112 (MQ=255)
                                                                                                                                                                        ccAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGa                                                    >  1:716856/1‑237 (MQ=255)
                                                                                                                                                                              aTCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGt                                                                                                                                        <  1:563370/147‑1 (MQ=255)
                                                                                                                                                                              aTCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGt                                                                                                                                        >  2:563370/1‑147 (MQ=255)
                                                                                                                                                                               tCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACt                                                                                                                                  <  2:380656/152‑1 (MQ=255)
                                                                                                                                                                               tCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACt                                                                                                                                  >  1:380656/1‑152 (MQ=255)
                                                                                                                                                                               tCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCaa                                                         <  2:308617/225‑1 (MQ=255)
                                                                                                                                                                               tCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCaa                                                         >  1:308617/1‑225 (MQ=255)
                                                                                                                                                                                   ttCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTc                                         >  1:38589/1‑237 (MQ=255)
                                                                                                                                                                                    tCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCg                                        <  2:38589/237‑1 (MQ=255)
                                                                                                                                                                                    tCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCt                                      <  1:6720/239‑1 (MQ=255)
                                                                                                                                                                                               ttCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGc                                                                                                                 <  1:374273/153‑1 (MQ=255)
                                                                                                                                                                                               ttCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGc                                                                                                                 >  2:374273/1‑153 (MQ=255)
                                                                                                                                                                                               ttCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGa                           >  2:681350/1‑239 (MQ=255)
                                                                                                                                                                                                  aaTGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAAc                        >  1:47822/1‑239 (MQ=255)
                                                                                                                                                                                                   aTGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTc                                                                                                                                                                                             >  1:274119/1‑78 (MQ=255)
                                                                                                                                                                                                   aTGGTCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTc                                                                                                                                                                                             <  2:274119/78‑1 (MQ=255)
                                                                                                                                                                                                       tCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACttt                                                                                                                                                                    <  1:234651/99‑1 (MQ=255)
                                                                                                                                                                                                       tCACCAGACCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACttt                                                                                                                                                                    >  2:234651/1‑99 (MQ=255)
                                                                                                                                                                                                              aCCGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATGTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCgg            >  2:826775/1‑239 (MQ=255)
                                                                                                                                                                                                               ccGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTggg               <  1:716303/235‑1 (MQ=255)
                                                                                                                                                                                                               ccGGAAACCCCACCGAATTCGTCAAGAACGATCGCCATGTGGTAACGCGGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAATCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTggg               <  1:1163056/235‑1 (MQ=255)
                                                                                                                                                                                                                   aaaCCCCACCGAATGCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCg             <  1:1139494/233‑1 (MQ=255)
                                                                                                                                                                                                                        ccACCGAATTCGTCAAGACCGATCGCCATGTGGTAACGCTGAGAGCGAACCTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCgatgat  <  2:873159/239‑1 (MQ=255)
                                                                                                                                                                                                                                 tCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCt                                                                                                                       <  1:148170/113‑1 (MQ=255)
                                                                                                                                                                                                                                 tCGTCAAGAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCt                                                                                                                       >  2:148170/1‑113 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   
GCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTCGTCATACTCGTCTTCAATCTCACCAACAATCAGTTCCAGGATGTCTTCAATGGTCACCAGACCGGAAACCCCACCGAATTCGTCAATAACGATCGCCATGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACG‑‑AC‑‑‑AACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGGCGGACTCGATGAT  >  NC_000913/691048‑691502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: