Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,183,048 T→G L191F (TTA→TTC potC ← spermidine/putrescine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,183,0480TG97.7% 295.6 / ‑6.1 87L191F (TTA→TTCpotCspermidine/putrescine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (42/42);  minor base A (0/2);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGC  >  NC_000913/1182828‑1183279
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       
aTTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCgccgcc                                                                                                                                                                                                                       <  1:47739/239‑1 (MQ=255)
    aaCGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCg                                                                                                                                                                                                                   <  2:845622/239‑1 (MQ=255)
            ctttgtTACCGTGCGTTTTATCACGAGCAATAGGCTGGATGGCAAGAACCATCGCCAGCGACAGCCCCAGTAATATGGTTGCCAGCCAGTTAACTGCCGGCGAAACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCCCATCGTCCATCGACAGGGTAAATCTGAACACCCAGCCCGCCGCCCCCGCTGGCAtt                                                                                                                                                                                                           <  2:734802/236‑1 (MQ=255)
                       ttCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCCCCACCGCTGGCATTGCCAGTGGCAg                                                                                                                                                                                                >  1:58745/1‑239 (MQ=255)
                         cGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGaa                                                                                                                                                                                              >  1:542405/1‑239 (MQ=255)
                         cGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCCCAGGCATTGCCAGTGGCAGaa                                                                                                                                                                                              >  2:1193589/1‑239 (MQ=255)
                                     aGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTTCCAGTGGCAGAATGATTTTTCgac                                                                                                                                                                                  >  2:1025391/1‑237 (MQ=255)
                                         aTAAGCTGGCTGGCAATAACCATCCCCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAAt                                                                                                                                                                              <  2:440949/239‑1 (MQ=255)
                                          tAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACCTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATg                                                                                                                                                                             >  1:485026/1‑239 (MQ=255)
                                                 tctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGAGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGACAGTGGCAGAATGAATTTTCGCAAAAGGGGaaaa                                                                                                                                                                       >  2:765500/2‑237 (MQ=255)
                                                 gctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCg                                                                                                                                                                                                                   >  1:1063579/1‑194 (MQ=255)
                                                 gctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCg                                                                                                                                                                                                                   <  2:1063579/194‑1 (MQ=255)
                                                 gctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAAt                                                                                                                                                                       >  1:568924/1‑238 (MQ=255)
                                                  ctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTc                                                                                                                                                                     >  1:427518/1‑239 (MQ=255)
                                                               tCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGCGGCAGAAAGATTTTTCGCAGAATGGTAAATTCCCTGGCACCgaga                                                                                                                                                        >  1:430394/1‑239 (MQ=255)
                                                                cACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAATAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTAGCTAGCACCaaga                                                                                                                                                        >  2:660634/1‑238 (MQ=255)
                                                                             aCCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgc                                                                                                                                                <  2:781133/233‑1 (MQ=255)
                                                                                            gCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgcc                                                                                                                                               >  2:155189/1‑219 (MQ=255)
                                                                                            gCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcgcc                                                                                                                                               <  1:155189/219‑1 (MQ=255)
                                                                                            gCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTc                                                                                                                           >  1:226628/1‑239 (MQ=255)
                                                                                            gCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGACGCTTCCAGCATTTGGACGTc                                                                                                                           >  2:563422/1‑239 (MQ=255)
                                                                                                         cTTCCGTCGATACGCCGCCTTTGACCATCGAATAAATTTTTAACGGCAAAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCCCATCGTCCATCGACGGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAgg                                                                                                              <  2:640166/239‑1 (MQ=255)
                                                                                                         cTTCCGGGGATACTCAGACTTTGACCAGCGAATAAATTTTTAACGGCAGAATTTCAGAACTTGGTCCGGGGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAgg                                                                                                              <  2:574996/239‑1 (MQ=255)
                                                                                                         cTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGGATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAgg                                                                                                              <  1:1091999/239‑1 (MQ=255)
                                                                                                         cTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAgg                                                                                                              <  1:764547/239‑1 (MQ=255)
                                                                                                                    aCGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCa                                                                                                                                                                                  <  1:412877/160‑1 (MQ=255)
                                                                                                                    aCGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCa                                                                                                                                                                                  >  2:412877/1‑160 (MQ=255)
                                                                                                                         gACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTca                                                                                              <  2:226628/239‑1 (MQ=255)
                                                                                                                          aCTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAgg                                                                                                              >  1:658307/1‑222 (MQ=255)
                                                                                                                          aCTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAgg                                                                                                              <  2:658307/222‑1 (MQ=255)
                                                                                                                          aCTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTca                                                                                              >  1:1097417/1‑238 (MQ=255)
                                                                                                                           cTTTGACCATCTAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAATATACCACTTCGTCCATCGACAGGGTAAAGCTGACCACCCACCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTcacc                                                                                            <  2:805969/239‑1 (MQ=255)
                                                                                                                           cTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTcacc                                                                                            <  1:194659/239‑1 (MQ=255)
                                                                                                                            ttggACCATCGAATAAATTTTTAACGGCAGATTTTCATAACTTGGCCCGGTCACGAACGAATAAACTACCACATCGCCCATCGCCGGGGTAAAGCTGAACCCCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTcacca                                                                                           <  2:427518/236‑1 (MQ=255)
                                                                                                                             ttGACCATCGAATAAATTTTTAACGGAAGAATTTCAAACCTTGTGCCGGTGACGAACGAAGAAACTACCCCATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTcaccac                                                                                          <  2:568924/239‑1 (MQ=255)
                                                                                                                              tGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATaca                                                                                                    >  2:368377/1‑228 (MQ=255)
                                                                                                                              tGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATaca                                                                                                    <  1:368377/228‑1 (MQ=255)
                                                                                                                              tGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTcaccacc                                                                                         >  1:1194393/1‑239 (MQ=255)
                                                                                                                                aCCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACAcc                                                                                                  >  2:120001/1‑228 (MQ=255)
                                                                                                                                aCCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACAcc                                                                                                  <  1:120001/228‑1 (MQ=255)
                                                                                                                                  cATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcg                                                                                                                                                 <  2:235772/179‑1 (MQ=255)
                                                                                                                                  cATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTcg                                                                                                                                                 >  1:235772/1‑179 (MQ=255)
                                                                                                                                     cGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCgccgcc                                                                                                                                                                                                                       <  2:844523/106‑1 (MQ=255)
                                                                                                                                     cGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCgccgcc                                                                                                                                                                                                                       >  1:844523/1‑106 (MQ=255)
                                                                                                                                      gAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGAAAAGCTGAACCCCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATgg                                                                                 <  1:579438/239‑1 (MQ=255)
                                                                                                                                          aaaTTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATggcagg                                                                             <  1:615970/239‑1 (MQ=255)
                                                                                                                                             tttttAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTCCCACATCGTCCATCGACAGGGTAAAGCTGAACCCCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGgcaggcag                                                                          <  1:991723/239‑1 (MQ=255)
                                                                                                                                               tttAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGgcaggcag                                                                          >  1:462788/1‑237 (MQ=255)
                                                                                                                                               tttAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGgcaggcag                                                                          <  2:462788/237‑1 (MQ=255)
                                                                                                                                                  aaCGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGt                                                                     >  2:331906/1‑239 (MQ=255)
                                                                                                                                                        atatgttCATTACTTGGTGCGGTGAAGAACGAACAACCTACCACATCGGCCATGCACGGGGTAAGCCTAAAACCCCTGCCCGCCGCCACCGCTGGCATTGCCGGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGa                                                                   <  2:1071623/230‑1 (MQ=255)
                                                                                                                                                           aTTTCATAACTTGGTCCGGTGACGAACGAAGAAACTCCCACATCGTCCATCGACAGGGTAAAGCTGAACCCCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGatat                                                                <  1:85230/235‑1 (MQ=255)
                                                                                                                                                                   actgagTCGGGTGCCGAAAGAAAAAACTCCCCCATCAGCAATAGTAGGGTGAAAGCGCACCGCCCAGCCCGCCGCCGCAGCTGGCATTGCCAGTGGCCGAATGATTTTTCGCAGAATGGTAAATTCGCAGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAAcagca                                                    <  2:945671/234‑1 (MQ=255)
                                                                                                                                                                    cTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCtt                                                                                                                                                    <  1:1068726/142‑1 (MQ=255)
                                                                                                                                                                    cTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCtt                                                                                                                                                    >  2:1068726/1‑142 (MQ=255)
                                                                                                                                                                       ggTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCa                                                                                                                >  2:551777/1‑175 (MQ=255)
                                                                                                                                                                       ggTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCa                                                                                                                <  1:551777/175‑1 (MQ=255)
                                                                                                                                                                       ggTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAAcaggcgag                                                 >  1:39468/1‑233 (MQ=255)
                                                                                                                                                                       ggTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCg                                                 >  1:1169905/1‑238 (MQ=255)
                                                                                                                                                                         tCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGAcc                                              >  1:377509/1‑239 (MQ=255)
                                                                                                                                                                          ccGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGAcc                                              <  1:1140923/238‑1 (MQ=255)
                                                                                                                                                                          ccGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCa                                             >  2:994392/1‑239 (MQ=255)
                                                                                                                                                                                 cgaacgaaGAAACTACCACGTCGTCCTTCGACAGGGTAAAGCTGAACACCAAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGcc                                       <  1:1155393/238‑1 (MQ=255)
                                                                                                                                                                                 cgaacgaaGAAACTACCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGcc                                       <  2:1187805/238‑1 (MQ=255)
                                                                                                                                                                                  gaagaaagaaACTCCCACACCGTCCAGCGACGGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTTGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCaa                                     <  2:1069979/234‑1 (MQ=255)
                                                                                                                                                                                              tcccccctCGTCCAGCGAAAGGGTAACGACAAACCCCGAGCCAGCCCCCGCCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCca                         <  2:58745/232‑1 (MQ=255)
                                                                                                                                                                                               aCCACATCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCca                         >  2:1188136/1‑238 (MQ=255)
                                                                                                                                                                                                ccaaatcgcataTCGACAGGGTAACGCTGAACCCCACGCCCGCCGCCAACGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCcagc                       <  2:1194126/228‑1 (MQ=255)
                                                                                                                                                                                                    aTCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCg                                                                                                                                                                                    >  1:951203/1‑78 (MQ=255)
                                                                                                                                                                                                    aTCGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCg                                                                                                                                                                                    <  2:951203/78‑1 (MQ=255)
                                                                                                                                                                                                      cGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATa                 >  1:541115/1‑239 (MQ=255)
                                                                                                                                                                                                      cGTCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAACCGAAATACCCCACAGCATa                 >  2:770050/1‑239 (MQ=255)
                                                                                                                                                                                                        tCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAgg                                                                                                              <  1:103909/144‑1 (MQ=255)
                                                                                                                                                                                                        tCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAgg                                                                                                              >  2:103909/1‑144 (MQ=255)
                                                                                                                                                                                                        tCCATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAATGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATaa                >  1:341252/1‑238 (MQ=255)
                                                                                                                                                                                                          cATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCa                                                                                                                                                                                  <  2:1050395/74‑1 (MQ=255)
                                                                                                                                                                                                          cATCGACAGGGTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCa                                                                                                                                                                                  >  1:1050395/1‑74 (MQ=255)
                                                                                                                                                                                                                  gggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGcc                                       <  1:829401/205‑1 (MQ=255)
                                                                                                                                                                                                                  gggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGcc                                       >  2:829401/1‑205 (MQ=255)
                                                                                                                                                                                                                  gggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATa                             >  2:345370/1‑215 (MQ=255)
                                                                                                                                                                                                                  gggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATa                             <  1:345370/215‑1 (MQ=255)
                                                                                                                                                                                                                   ggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCg                                                                                                                                                                                    <  2:730406/63‑1 (MQ=255)
                                                                                                                                                                                                                   ggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCg                                                                                                                                                                                    >  1:730406/1‑63 (MQ=255)
                                                                                                                                                                                                                   ggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGgcaggcag                                                                          <  1:942070/169‑1 (MQ=255)
                                                                                                                                                                                                                   ggTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGgcaggcag                                                                          >  2:942070/1‑169 (MQ=255)
                                                                                                                                                                                                                    gTAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCAc         <  1:994392/233‑1 (MQ=255)
                                                                                                                                                                                                                     tAAAGCTGAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCagcagc  >  1:1032853/1‑239 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       
ATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCGCCGCCACCGCTGGCATTGCCAGTGGCAGAATGATTTTTCGCAGAATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGC  >  NC_000913/1182828‑1183279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: