Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 95,418 T→G S257A (TCG→GCG)  murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091395,4180TG100.0% 247.3 / NA 69S257A (TCG→GCG) murFUDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (38/31);  total (38/31)

GCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCTACAACG  >  NC_000913/95184‑95652
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          
gggccggTCAACAACCTGGCAGCGTCCCATCGTGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGGGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCGCCCAATGCCGCAAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                        <  2:708723/234‑1 (MQ=255)
gcgcTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                        <  1:310239/239‑1 (MQ=255)
 cgcTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGCGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGCGTGGCGTTTCTCACCCAATGCCGCCAAAAGCGATTTCACCGCCACCAATATCCATGTAACCGCGCAc                                                                                                                                                                                                                                       >  1:208895/1‑239 (MQ=255)
         aacaacCTGGCATGGGGGCATCTGGAAGGTTTTGGCTGGCTTGCGGGTGTCGCGAAAGCGAAAGGGGAAATCTTTAGCGGCCTGCCGAAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCTTTTCTCCCCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                        <  2:368469/230‑1 (MQ=255)
             acCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAAttt                                                                                                                                                                                                                           <  2:1038977/239‑1 (MQ=255)
                   cAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGAGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGaa                                                                                                                                                                                                                              >  1:368469/1‑230 (MQ=255)
                                   agtttttGGCTGGATTGCGGTGTTCGCGAAAGGGAAAGGTAAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGt                                                                                                                                                                                                      <  1:422094/235‑1 (MQ=255)
                                        ttGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTc                                                                                                                                                                                                >  1:75755/1‑239 (MQ=255)
                                                           cgcgAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAAt                                                                                                                                                                                                                             >  1:322251/1‑191 (MQ=255)
                                                           cgcgAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAAt                                                                                                                                                                                                                             <  2:322251/191‑1 (MQ=255)
                                                                    gaaagGGGAATTTTTTAGCGGCCTACCGGAAAACAGTAACGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTATTTGGCTCACGAATAGTGGGGCGTTTCTCCCCAAATGCCGCCAACAGCAATTTCTCCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCTTTGCCGGGGCGTc                                                                                                                                                                    <  2:208895/239‑1 (MQ=255)
                                                                                                  aaaCGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTg                                                                                                                                      <  2:75755/239‑1 (MQ=255)
                                                                                                      ggTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCCCGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGct                                                                                                                                  <  1:1074780/239‑1 (MQ=255)
                                                                                                       gTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACACTATTGCGAATGCGCTGGCAGCAGCTgcgcc                                                                                                                                  >  2:647971/1‑237 (MQ=255)
                                                                                                                ttATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTcc                                                                                                                        >  1:184980/1‑239 (MQ=255)
                                                                                                                       cGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGgc                                                                                                                  >  1:728086/1‑238 (MQ=255)
                                                                                                                        gCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAg                                                                                                                                                                                                    >  2:499673/1‑155 (MQ=255)
                                                                                                                        gCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAg                                                                                                                                                                                                    <  1:499673/155‑1 (MQ=255)
                                                                                                                          cGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCa                                                                                                               >  2:1104350/1‑238 (MQ=255)
                                                                                                                            acaacaACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGt                                                                                                                                                                                                 >  1:954206/1‑154 (MQ=255)
                                                                                                                            acaacaACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGt                                                                                                                                                                                                 <  2:954206/154‑1 (MQ=255)
                                                                                                                                  acGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                        >  1:445379/1‑109 (MQ=255)
                                                                                                                                  acGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                        <  2:445379/109‑1 (MQ=255)
                                                                                                                                   gggttGGCTGAACTGGCAGGGCGTAATTGGCTCACGCAAAGTGTGGCGTTCCTCACCCAATGCCCCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATg                                                                                                     <  2:55333/235‑1 (MQ=255)
                                                                                                                                      cTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                        >  1:444237/1‑105 (MQ=255)
                                                                                                                                      cTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                        <  2:444237/105‑1 (MQ=255)
                                                                                                                                       tGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCCCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTAt                                                                                                 <  1:1034707/239‑1 (MQ=255)
                                                                                                                                        ggCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACa                                                                                                                                                                                                                   >  1:1004153/1‑124 (MQ=255)
                                                                                                                                        ggCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACa                                                                                                                                                                                                                   <  2:1004153/124‑1 (MQ=255)
                                                                                                                                        ggCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAAccc                                                                                                                                                                                                              <  1:632765/129‑1 (MQ=255)
                                                                                                                                        ggCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAAccc                                                                                                                                                                                                              >  2:632765/1‑129 (MQ=255)
                                                                                                                                              aCTGGAAGAGCGTAATTGGCTCCCGCAAAGGTTGGCGTTTCTCCCCCAATGCCGCCAACAGCGATTTCCCCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGa                                                                                                       <  2:461697/226‑1 (MQ=255)
                                                                                                                                                tGGCAGAGCTTAATTGGCTCACGCAAAGTGGGGCGTTTCTCACCCAATCCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCggg                                                                                        <  2:1030489/239‑1 (MQ=255)
                                                                                                                                                   cAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCtgctgc                                                                                                                                                                                   >  2:332712/1‑145 (MQ=255)
                                                                                                                                                   cAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCCCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCtgctgc                                                                                                                                                                                   <  1:332712/145‑1 (MQ=255)
                                                                                                                                                       gCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCCCGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGGCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGCGGCGGGc                                                                                  >  1:593806/1‑238 (MQ=255)
                                                                                                                                                       gCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCa                                                                                 >  2:772130/1‑239 (MQ=255)
                                                                                                                                                        cGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCcaa                                                                                             >  1:461697/1‑226 (MQ=255)
                                                                                                                                                        cGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCaa                                                                                >  2:734001/1‑239 (MQ=255)
                                                                                                                                                        cGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCaa                                                                                >  1:1030489/1‑239 (MQ=255)
                                                                                                                                                          tAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAAt                                                                                                                                                                                                                             <  2:1063003/96‑1 (MQ=255)
                                                                                                                                                          tAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAAt                                                                                                                                                                                                                             >  1:1063003/1‑96 (MQ=255)
                                                                                                                                                          tAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCaaa                                                                               >  1:269679/1‑238 (MQ=255)
                                                                                                                                                                 cTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGaaa                                                                        >  1:719378/1‑238 (MQ=255)
                                                                                                                                                                     cGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATgcg                                                                                                                                                   >  2:609088/1‑159 (MQ=255)
                                                                                                                                                                     cGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATgcg                                                                                                                                                   <  1:609088/159‑1 (MQ=255)
                                                                                                                                                                     cGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGaaa                                                                        >  2:222010/1‑234 (MQ=255)
                                                                                                                                                                     cGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAAACTGAAAGCTgg                                                                   >  1:1158908/1‑238 (MQ=255)
                                                                                                                                                                          aGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAg                                                              >  2:599900/1‑239 (MQ=255)
                                                                                                                                                                              tgGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGCTGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAACTCTGAAAGCTGTTCCAg                                                              >  1:1061017/1‑235 (MQ=255)
                                                                                                                                                                                gCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCggg                                                                                        <  2:763494/207‑1 (MQ=255)
                                                                                                                                                                                gCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCggg                                                                                        >  1:763494/1‑207 (MQ=255)
                                                                                                                                                                                 gtttccccccccAAGGCCGCCACCAGCAATTTCCCCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTc                                                        <  2:184980/229‑1 (MQ=255)
                                                                                                                                                                                               tGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCa                                           >  1:1113863/1‑237 (MQ=255)
                                                                                                                                                                                                  cgccAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACt                                        <  1:1104350/237‑1 (MQ=255)
                                                                                                                                                                                                   gccAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGc                                     >  1:771743/1‑239 (MQ=255)
                                                                                                                                                                                                           cGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAAcc                             >  2:1019056/1‑239 (MQ=255)
                                                                                                                                                                                                               ttCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGtt                         >  1:653833/1‑239 (MQ=255)
                                                                                                                                                                                                                tCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTtg                        <  1:772130/239‑1 (MQ=255)
                                                                                                                                                                                                                   ccGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCa                                                                                 <  2:65767/179‑1 (MQ=255)
                                                                                                                                                                                                                   ccGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCa                                                                                 >  1:65767/1‑179 (MQ=255)
                                                                                                                                                                                                                   ccGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGtt                         >  2:492903/1‑235 (MQ=255)
                                                                                                                                                                                                                       ccccaATCTCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAGCCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTcg                 <  1:1185168/237‑1 (MQ=255)
                                                                                                                                                                                                                        accaATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCCcg                 >  2:1185168/1‑238 (MQ=255)
                                                                                                                                                                                                                             tatCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACt           <  2:1113863/239‑1 (MQ=255)
                                                                                                                                                                                                                               tCCATGTGACCGCGCACGGTACGGAATTTACCCTa                                                                                                                                                                                                                     <  1:62696/35‑1 (MQ=255)
                                                                                                                                                                                                                                    gtgACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTcc                                                  >  1:508533/1‑193 (MQ=255)
                                                                                                                                                                                                                                    gtgACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTcc                                                  <  2:508533/193‑1 (MQ=255)
                                                                                                                                                                                                                                       aCCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCTACAACg  >  2:895981/1‑238 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          
GCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCTACAACG  >  NC_000913/95184‑95652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: