Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	109,887	A→C	I537L (ATT→CTT)	secA →	preprotein translocase subunit, ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	109,887	0	A	C	100.0%	215.9 / NA	56	I537L (ATT→CTT)	secA	preprotein translocase subunit, ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/40);  total (16/40)

AAATCGGAGCTGGTGTCAAACGAACTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCG  >  NC_000913/109659‑110091                                                                                                                                                                                                                                     |                                                                                                                                                                                                             aaaTCGGAGCTGGTGTCAAACGAACTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGa                                                                                                                                                                                                    <  1:998356/239‑1 (MQ=255)      gcaGCTGGTGTCAAACGAACTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGc                                                                                                                                                                                               <  1:62326/237‑1 (MQ=255)               gtCAAACGAACTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGt                                                                                                                                                                                       <  1:1160833/238‑1 (MQ=255)                 cAAACGAACTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCa                                                                                                                                                                                     <  1:962352/238‑1 (MQ=255)                 cAAACGAACTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCa                                                                                                                                                                                     <  1:355653/238‑1 (MQ=255)                     cGAACTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATg                                                                                                                                                                                <  1:684242/239‑1 (MQ=255)                      gAACTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGc                                                                                                                                                                               <  1:319160/239‑1 (MQ=255)                        aCTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCg                                                                                                                                                                              <  1:479726/238‑1 (MQ=255)                                        aTTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTg                                                                                                                                                              <  1:1019316/238‑1 (MQ=255)                                           aaGGACAACGTCCTTAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTAAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGc                                                                                                                                                            <  1:755073/237‑1 (MQ=255)                                               gcaACGGCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      <  1:972692/238‑1 (MQ=255)                                               acaACGTGCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      <  2:799448/239‑1 (MQ=255)                                               acaACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      <  2:1167116/239‑1 (MQ=255)                                               acaACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      <  1:665727/239‑1 (MQ=255)                                                       cTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCg                                                                                                                                               <  1:19754/238‑1 (MQ=255)                                                               cAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGc                                                                                                                                      <  2:994496/239‑1 (MQ=255)                                                                    tCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATatcat                                                                                                                                                 <  1:1025421/223‑1 (MQ=255)                                                                           cAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATcc                                                                                                                           <  1:661477/238‑1 (MQ=255)                                                                               gAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCcgtcg                                                                                                                      <  1:206431/239‑1 (MQ=255)                                                                                          tgttgCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTa                                                                                                                                                                           <  1:669418/175‑1 (MQ=255)                                                                                                         ttATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGATgcgc                                                                                                   <  1:1177167/232‑1 (MQ=255)                                                                                                          tATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCg                                                                                                                                                                              <  1:297188/156‑1 (MQ=255)                                                                                                                     ggTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAgg                                                                                <  1:315684/239‑1 (MQ=255)                                                                                                                       tGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAggg                                                                               <  2:557871/238‑1 (MQ=255)                                                                                                                                     aaTATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCTACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTgg                                                                                                                                                             <  1:970516/146‑1 (MQ=255)                                                                                                                                               gTCGTGGTCCAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTa                                                       <  1:819781/238‑1 (MQ=255)                                                                                                                                                tCGGGGTACAGATATTGTGCTCGGTGGGAGCTGGCAGGCAGAAGTTGCCGCGGTGGAAAATCCGACCGCAGAACAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTTTCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGAGGCTGGTTCTTCCCGTTTCTAc                                                      <  2:1098871/238‑1 (MQ=255)                                                                                                                                                 cGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGt                                                                                                                                                                                       <  1:201148/108‑1 (MQ=255)                                                                                                                                                    ggTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTc                                                 <  1:61304/239‑1 (MQ=255)                                                                                                                                                         aGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCCTCCCGAATCCCGCCGTAACGATAACCAGTTGCGCGGTCGTTCTGGTGGTCAGGGGGATGATGGGTCTTGCCTGTTCTACCTTTCGAAgg                                            >  2:312107/1‑239 (MQ=255)                                                                                                                                                           atatTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCTGCGCTGGAAAATCCGACCGCAGGGCAAATTGAAAAACTTAAAGCCGACCGGCAGGGCCGTCAAGATGCgg                                                                                                                                                                             >  2:201148/1‑108 (MQ=255)                                                                                                                                                                gtgCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTg                                                                                         >  1:501691/1‑187 (MQ=255)                                                                                                                                                                gtgCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTg                                                                                         <  2:501691/187‑1 (MQ=255)                                                                                                                                                                gtgCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTACGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATgc                                     >  1:1058410/1‑239 (MQ=255)                                                                                                                                                                  gCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATgcg                                    <  1:76575/238‑1 (MQ=255)                                                                                                                                                                   cTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATgc                                     >  1:257724/1‑236 (MQ=255)                                                                                                                                                                   cTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGCCACGATGCAGTACTGGAAGCAGGTCGCCTGCCTAATATCGGCACCGCGCGTCCCGAAACCCGGTGTTACGCTAACCAGTTGCGCGGGCGTTTTGGTCGACAGGGGGATGCCGGGTATTCTCGTTTATACCTTTTGATGGAAGAATCGCt                                  >  2:1007183/1‑239 (MQ=255)                                                                                                                                                                    tCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGACTACATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGGCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATgc                                     >  1:901255/1‑235 (MQ=255)                                                                                                                                                                     cGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      <  2:214960/121‑1 (MQ=255)                                                                                                                                                                     cGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      >  1:214960/1‑121 (MQ=255)                                                                                                                                                                      ggtggtAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      <  1:154668/120‑1 (MQ=255)                                                                                                                                                                      ggtggtAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      >  2:154668/1‑120 (MQ=255)                                                                                                                                                                           tAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGt                           <  2:257724/238‑1 (MQ=255)                                                                                                                                                                                ggcaggcaGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGcgt                                                                                                                                                      <  2:1042411/110‑3 (MQ=255)                                                                                                                                                                                ggcaggcaGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGcgt                                                                                                                                                      >  1:1042411/1‑108 (MQ=255)                                                                                                                                                                                  caggcagAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGAc                                                                                                                                                                                                   <  2:36982/63‑1 (MQ=255)                                                                                                                                                                                  caggcagAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGAc                                                                                                                                                                                                   >  1:36982/1‑63 (MQ=255)                                                                                                                                                                                  caggcagAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAggg                                                                               >  1:1001863/1‑179 (MQ=255)                                                                                                                                                                                  caggcagAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAggg                                                                               <  2:1001863/179‑1 (MQ=255)                                                                                                                                                                                  caggcagAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTg                                 >  2:1014771/1‑225 (MQ=255)                                                                                                                                                                                  caggcagAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTg                                 <  1:1014771/225‑1 (MQ=255)                                                                                                                                                                                         aaGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCg                                                                                                                                                                              <  2:400612/77‑1 (MQ=255)                                                                                                                                                                                           gTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGGGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGGCGGATCGATAAACAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGGAGGTGGATCTTCCCGGTTCTACCCGTCGATGGGAGATGCGCTGATGCGGATTTTTGGTTccgaccg          >  2:1173749/1‑239 (MQ=255)                                                                                                                                                                                                   gcTGGAAAATCCGACCGCAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGGGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTTGATGGAAGATGCGCTGATGCGTATTTTTGTTTCCGACCGCGTATCCg  >  2:771012/1‑239 (MQ=255)                                                                                                                                                                                                                    cAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      <  2:1149529/74‑1 (MQ=255)                                                                                                                                                                                                                    cAGAGCAAATTGAAAAACTTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCat                                                                                                                                                      >  1:1149529/1‑74 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                             AAATCGGAGCTGGTGTCAAACGAACTGACCAAAGCCGGTATTAAGCACAACGTCCTGAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCG  >  NC_000913/109659‑110091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑