Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,667,681	T→G	S56R (AGT→CGT)	hcaT ←	putative 3‑phenylpropionic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,667,681	0	T	G	100.0%	250.5 / NA	72	S56R (AGT→CGT)	hcaT	putative 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/35);  total (37/35)

GCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACTCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAACCATGAGTCCCTCCCGTCAGGAGGCGTTAGTCTGGGGTTAATCGAATGTTGGTGCAAGTTGAAAAATAAGA  >  NC_000913/2667468‑2667913                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         gCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCt                                                                                                                                                                                                                 >  1:1042384/1‑239 (MQ=255) gCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCAACGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGAACGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAAACAGa                                                                                                                                                                                                                    >  1:1134078/1‑236 (MQ=255)   gggAACTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTg                                                                                                                                                                                                                <  1:77798/238‑1 (MQ=255)     gAACTGATTTTTCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGcc                                                                                                                                                                                                              <  2:894040/238‑1 (MQ=255)       aCTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGCCCTGccccc                                                                                                                                                                                                           >  1:588249/1‑239 (MQ=255)       aCTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGcccc                                                                                                                                                                                                            <  2:1145396/238‑1 (MQ=255)            ttttGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATaa                                                                                                                                                                                                      <  1:694727/239‑1 (MQ=255)            ttttGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATaa                                                                                                                                                                                                      <  1:1133877/239‑1 (MQ=255)                gCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCCAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGCAACGGGCAACCAGACCTGCCCCCACTAACAg                                                                                                                                                                                                   >  1:755013/1‑238 (MQ=255)                gCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAgg                                                                                                                                                                                                  <  1:606655/239‑1 (MQ=255)                                tGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCCCCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCCTCCCCCAATAACACGGCGGAGGATTCTGGCg                                                                                                                                                                                  >  2:238430/1‑239 (MQ=255)                                tGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCg                                                                                                                                                                                  >  2:689923/1‑239 (MQ=255)                                tGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGCGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGCAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGAGGGTTTCTGGCg                                                                                                                                                                                  >  1:858086/1‑239 (MQ=255)                                 gCATCGGTCAACGTTACCAGTGGTGAGAAAAAGGGGTTAAAGCCATTCATCACCAGCATCAGCCCCGCTACGGGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGt                                                                                                                                                                                 <  1:934158/239‑1 (MQ=255)                                          aaCGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAAt                                                                                                                                                                        <  2:362678/239‑1 (MQ=255)                                               tACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATc                                                                                                                                                                       >  1:403096/1‑235 (MQ=255)                                                      ggtgAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCa                                                                                                                                                            >  2:199427/1‑239 (MQ=255)                                                       gtgAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACgg                                                                                                                                                                                                                             >  1:855301/1‑173 (MQ=255)                                                       gtgAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACgg                                                                                                                                                                                                                             <  2:855301/173‑1 (MQ=255)                                                         gagaAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCCGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAAACAATCCCTTTAAGCCAGa                                                                                                                                                          >  2:1096073/1‑238 (MQ=255)                                                         gagaAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGAc                                                                                                                                                         >  1:19195/1‑239 (MQ=255)                                                           gaAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGc                                                                                                                                                       >  2:71247/1‑239 (MQ=255)                                                               aaGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACGGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTCAGCCAGACGCTCCa                                                                                                                                                   >  2:716095/1‑239 (MQ=255)                                                               aaGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCa                                                                                                                                                   >  1:53086/1‑239 (MQ=255)                                                                      tAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAgg                                                                                                                                             <  1:949679/238‑1 (MQ=255)                                                                               tcatcaCCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATg                                                                                                                                   <  2:1042384/239‑1 (MQ=255)                                                                                   caCCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGt                                                                                                                               <  2:53086/239‑1 (MQ=255)                                                                                   caCCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGt                                                                                                                               <  2:403096/239‑1 (MQ=255)                                                                                   caCCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGt                                                                                                                               <  1:1194436/239‑1 (MQ=255)                                                                                         cATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTg                                                                                                                                                                                                                >  1:277838/1‑152 (MQ=255)                                                                                         cATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTg                                                                                                                                                                                                                <  2:277838/152‑1 (MQ=255)                                                                                          aTCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACt                                                                                                                           <  1:71247/236‑1 (MQ=255)                                                                                                         tGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAAt                                                                                                                                                                        <  1:633113/176‑1 (MQ=255)                                                                                                         tGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAAt                                                                                                                                                                        >  2:633113/1‑176 (MQ=255)                                                                                                         tGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGa                                                                                                                                                          >  1:75041/1‑190 (MQ=255)                                                                                                         tGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGa                                                                                                                                                          <  2:75041/190‑1 (MQ=255)                                                                                                         tGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAgg                                                                                                                                             >  1:163566/1‑203 (MQ=255)                                                                                                         tGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAgg                                                                                                                                             <  2:163566/203‑1 (MQ=255)                                                                                                             cccccGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAgg                                                                                                                                                                                                  >  2:1022872/1‑146 (MQ=255)                                                                                                             cccccGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAgg                                                                                                                                                                                                  <  1:1022872/146‑1 (MQ=255)                                                                                                             cccccGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCCATCCCTTTAAGCCAGACGCTCCAGAAATGTAGAAAAATGCCGTAACTACAAAAGTATGTGAAATAGACg                                                                                                     >  2:625047/1‑239 (MQ=255)                                                                                                             cccccGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGCATGTGAAATAGCCg                                                                                                     >  1:632298/1‑239 (MQ=255)                                                                                                             cccccGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCCCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGGTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCg                                                                                                     >  2:61213/1‑239 (MQ=255)                                                                                                                    ccAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGcc                                                                                               <  2:552860/238‑1 (MQ=255)                                                                                                                         aGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCaa                                                                                             <  2:156726/235‑1 (MQ=255)                                                                                                                          ggCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCCgc                                                                                        >  2:242220/1‑239 (MQ=255)                                                                                                                             gACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGcc                                                                                                                                 <  1:739253/195‑1 (MQ=255)                                                                                                                             gACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGcc                                                                                                                                 >  2:739253/1‑195 (MQ=255)                                                                                                                              aCAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCCGCGCGt                                                                                    >  1:202952/1‑239 (MQ=255)                                                                                                                                            tgtCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGcccc                                                                                                                                                                                                            <  2:1047662/105‑1 (MQ=255)                                                                                                                                            tgtCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGcccc                                                                                                                                                                                                            >  1:1047662/1‑105 (MQ=255)                                                                                                                                            tgtCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAgg                                                                                                                                                                                                  <  1:1030604/115‑1 (MQ=255)                                                                                                                                            tgtCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAgg                                                                                                                                                                                                  >  2:1030604/1‑115 (MQ=255)                                                                                                                                                 gcagTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCCCCAGAAAGGGAGAAAAATGCCGTAACTAAAAAAGGATGTGAAATAGCCGAGCGCCAACCCGCGCGAGGATTGCAAAACCATGAg                                                                  >  1:1105730/1‑238 (MQ=255)                                                                                                                                                   aggggcAGCACGCGCAAGGCGGAAATCAGGGGGGAAGGGTCCCTGACGCGGGGCCCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGGCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCCGCGCGTGGATTGCAAAACCa                                                                      <  2:1134078/227‑1 (MQ=255)                                                                                                                                                   agTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGa                                                                                                                                                          >  1:1032252/1‑148 (MQ=255)                                                                                                                                                   agTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGa                                                                                                                                                          <  2:1032252/148‑1 (MQ=255)                                                                                                                                                        cAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAAt                                                                                                                                                                        >  2:33445/1‑129 (MQ=255)                                                                                                                                                        cAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAAt                                                                                                                                                                        <  1:33445/129‑1 (MQ=255)                                                                                                                                                          gCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                                                                                           <  2:80214/188‑1 (MQ=255)                                                                                                                                                          gCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                                                                                           >  1:80214/1‑188 (MQ=255)                                                                                                                                                               cgcAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGa                                                                                                                                                 >  2:759634/1‑145 (MQ=255)                                                                                                                                                               cgcAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGa                                                                                                                                                 <  1:759634/145‑1 (MQ=255)                                                                                                                                                               cgcAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTAtg                                                                                                                >  2:941570/1‑178 (MQ=255)                                                                                                                                                               cgcAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTAtg                                                                                                                <  1:941570/178‑1 (MQ=255)                                                                                                                                                               cgcAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCCGCGCGTGGATTGCAAAACCATGAGTCCCTCCCGTCagga                                                   >  1:271981/1‑239 (MQ=255)                                                                                                                                                               cgcAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCCGCGCGTGGATTGCAAAACCATGAGTCCCTCCCGTCagga                                                   >  2:1038196/1‑239 (MQ=255)                                                                                                                                                                   aGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCCGCGCGTGGATTGCAAAACCATGAGTCCCTCCCGTCAGGAGGCg                                               <  2:202952/239‑1 (MQ=255)                                                                                                                                                                   aGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCCGCGCGTGGATTGCAAAACCATGAGTCCCTCCCGGCAGGAGGCg                                               <  1:199427/239‑1 (MQ=255)                                                                                                                                                                          aaTCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCCGCGCGTGGATTGCAAAACCATGAGTCCCTCCCGTCAGGAGGCGTTAGTCt                                        <  2:588249/239‑1 (MQ=255)                                                                                                                                                                                                                gCAAACGCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCCGCGCGTGGATTGCACAACCATGAGTCCCTCCCGTCAGGAGGCGTTAGTCTGGGGTTAATCGAATGTTGTGGCAAGTTGAAAAATAAGa  >  2:579092/1‑239 (MQ=255)                                                                                                                                                                                                                  aaaCGCCCGAGGAAACGGGCAACCAGCCCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAGCCCGCGCGTGGATTGCAAAACCATGAGTCCCGCCCGTCAGGAGGCGTTAGTCTGGGGTTAATCGAATGTTGGTGCAAGTTGAAAAATAAGa  <  1:238430/237‑1 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         GCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCATCGGTCAACGGTACCAGTGGTGAGAAAAAGAGGTTAAAGCCAATCATCACCAGCATCAGCCACGCTACGTGCGCCCCCGCCCAGAAGGCGACAGCAAAGAGAAGTGTCAGCAGTGCCAGCACGCGCAAGGCGGAAATCAGGCGGGAAGGATCGCTGACGCGGGGCGCGATGAGCAAACTCCCGAGGAAACGGGCAACCAGACCTGCCCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAACCATGAGTCCCTCCCGTCAGGAGGCGTTAGTCTGGGGTTAATCGAATGTTGGTGCAAGTTGAAAAATAAGA  >  NC_000913/2667468‑2667913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑