Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,912,404	A→C	V416V (GTT→GTG)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,912,404	0	A	C	100.0%	214.7 / NA	59	V416V (GTT→GTG)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/32);  total (27/32)

GAACGCCCACGGCTGGTTGTGACGTAACCGAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCAT  >  NC_000913/2912183‑2912625                                                                                                                                                                                                                              |                                                                                                                                                                                                                               gaccGCCCAGGGTTGGTTGGGACGTACCCGGTGTTTGGTTTAAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCttt                                                                                                                                                                                                                  <  1:168205/233‑1 (MQ=255) gAACGCCGACGGCTGGTTGTGACGTAACCGAGGTTTGGTTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCttt                                                                                                                                                                                                                  <  1:265365/236‑1 (MQ=255)          cGGCTGGTTGTGACGTAACCGAGTTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCg                                                                                                                                                                                                             <  1:765110/232‑1 (MQ=255)                 ttGTGACGTAACCGAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGAc                                                                                                                                                                                                      <  1:17835/232‑1 (MQ=255)                     gACGTAACCGAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTa                                                                                                                                                                                                   >  1:444386/1‑231 (MQ=255)                                                gTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTAAACCCGGCGGTCACAGACCTGACTACGTACt                                                                                                                                                                    >  2:308927/1‑235 (MQ=255)                                                           ggTTAGGCTGTTTCTGGGTGATAATTTCAATCGGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAAGGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCAt                                                                                                                                                         <  1:407051/235‑1 (MQ=255)                                                                        gtggatcaaattttCACTTTGGTCGCCCATCTCCGGGTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAg                                                                                                                                           <  2:141548/225‑1 (MQ=255)                                                                               aTAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCAtct                                                                                                                                     >  1:306846/1‑235 (MQ=255)                                                                                   tttCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTc                                                                                                                                 <  1:499369/235‑1 (MQ=255)                                                                                        aTCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCa                                                                                                                           <  1:516963/236‑1 (MQ=255)                                                                                             gccGCCCATCTGCAGCTGGTAGGGAACCGGCCCAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCaa                                                                                                                      <  1:625957/234‑1 (MQ=255)                                                                                             gTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCaa                                                                                                                      >  2:305302/1‑236 (MQ=255)                                                                                              tCGTCCTTCTGCAGCTGGAGAGAGAACGGCCCATTGCCACCGACAATTTTGGCCCCGATGCCGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGc                                                                                                                        <  2:444386/233‑1 (MQ=255)                                                                                                     tCTGCAGCTGTGAGGTAACCGGCACAGTGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTTGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTa                                                                                                              <  1:86822/236‑1 (MQ=255)                                                                                                     tCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTa                                                                                                              >  1:40326/1‑236 (MQ=255)                                                                                                     tCTGCAGCTGGAAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCACGATGCAGCGGTGTCCGACATCACGGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTa                                                                                                              <  1:308927/236‑1 (MQ=255)                                                                                                                        cGGCAAATTGCCACCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCgg                                                                                            <  2:306846/235‑1 (MQ=255)                                                                                                                              aaTGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCa                                                                                      <  1:141594/235‑1 (MQ=255)                                                                                                                                     ccgccAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTGCGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGa                                                                              >  1:134569/1‑236 (MQ=255)                                                                                                                                     ccgccAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGCGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGCCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCGTCCTGCCACTCAATCAGGTTACGCAGCCAGGCAATCCGGGCTTCATGTCCCg                                                                               >  2:132280/1‑235 (MQ=255)                                                                                                                                               cctaccagctGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGcgcc                                                                    <  1:601205/226‑1 (MQ=255)                                                                                                                                                 gCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCgccgcc                                                                 >  1:311464/1‑236 (MQ=255)                                                                                                                                                       aTGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑TTGCGACGCcatcacc                                                           >  2:537474/1‑234 (MQ=255)                                                                                                                                                           aGCGGTGTCCGACATCCCTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCCCCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCcgcg                                                       <  1:61973/236‑1 (MQ=255)                                                                                                                                                           aGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCCCCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCcgcg                                                       <  1:260485/236‑1 (MQ=255)                                                                                                                                                            gCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCcgcg                                                       <  2:311464/235‑1 (MQ=255)                                                                                                                                                                tgtCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCACCAACCGTGccccc                                                  >  2:612804/1‑234 (MQ=255)                                                                                                                                                                 ttCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGTCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCGGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCctc                                                  <  1:534938/234‑1 (MQ=255)                                                                                                                                                                 gtCCGACATCACTGTGGATGGGGTAAGCGAATTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGcc                                                     <  2:393435/232‑1 (MQ=255)                                                                                                                                                                 gaccGACCTCGCTGGTGATGTGGTAAGCGAAGGTCAGCGGCGTTGATCCCGCAAGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCct                                                   <  1:225498/232‑1 (MQ=255)                                                                                                                                                                 atCCGACATCACTTGTGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCctc                                                  <  2:86917/234‑1 (MQ=255)                                                                                                                                                                  tCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCctc                                                  <  1:485225/234‑1 (MQ=255)                                                                                                                                                                  tCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCctc                                                  >  2:485225/1‑234 (MQ=255)                                                                                                                                                                  tCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCCCtt                                                >  1:713178/1‑236 (MQ=255)                                                                                                                                                                  tCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCCCTGCCAGCAGCCGCGCCCGCtt                                                >  2:534938/1‑236 (MQ=255)                                                                                                                                                                          cACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCCCTTCCTGCCACGCAATCAGTTTACGCAGCCCGGCAATCCGGCCTTCATGTCCCGATCGGTGC‑TCGCCAGCAGCCCGGACCCCTTTAAAtt                                          >  2:534337/1‑234 (MQ=255)                                                                                                                                                                               tgGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTg                                   <  1:305302/236‑1 (MQ=255)                                                                                                                                                                               tgGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCCCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCCCCAGCAGCCGCGCCCCCCTTATATTTCCAATg                                   >  2:421660/1‑236 (MQ=255)                                                                                                                                                                                 gATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GGGCGCCGCCAGCAGCCGCGCCCCCTTAATATTTCCATTgcg                                 >  2:544627/1‑236 (MQ=255)                                                                                                                                                                                    gtgGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCgca                               >  1:99276/1‑235 (MQ=255)                                                                                                                                                                                    gtgGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGGTTAACCAGCCAGGCAATCCGGCCTTCATGTCCCGACC‑GTCCGCCGCCAGCAGCAGCGCCCTCGTTATATTTCCAATgccga                               >  2:651974/1‑232 (MQ=255)                                                                                                                                                                                     tgGTAAGCGAAGTCCAGCGGCGTGGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCgcag                              <  2:341404/235‑1 (MQ=255)                                                                                                                                                                                       gTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCcgcg                                                       <  1:164573/208‑1 (MQ=255)                                                                                                                                                                                       gTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCcgcg                                                       >  2:164573/1‑208 (MQ=255)                                                                                                                                                                                       gTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTCTCTTTCCAGtcggaagca                            >  2:18181/1‑227 (MQ=255)                                                                                                                                                                                       gTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTCCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCgcagca                            >  1:755788/1‑235 (MQ=255)                                                                                                                                                                                         aaGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAaca                         >  1:537291/1‑236 (MQ=255)                                                                                                                                                                                          aGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTCCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAaca                         <  2:755788/235‑1 (MQ=255)                                                                                                                                                                                                 tCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAActc                 >  2:762184/1‑236 (MQ=255)                                                                                                                                                                                                 tCCAGCGGCGTTGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAActc                 >  1:589818/1‑236 (MQ=255)                                                                                                                                                                                                           ttGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCctc                                                  >  2:441376/1‑193 (MQ=255)                                                                                                                                                                                                           ttGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCctc                                                  <  1:441376/193‑1 (MQ=255)                                                                                                                                                                                                           ttGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTg               <  2:432428/228‑1 (MQ=255)                                                                                                                                                                                                           ttGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTg               >  1:432428/1‑228 (MQ=255)                                                                                                                                                                                                           ttGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCa       >  1:602043/1‑236 (MQ=255)                                                                                                                                                                                                            tGATCCCGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCa       <  1:295997/235‑1 (MQ=255)                                                                                                                                                                                                            tGATCCCGAAGGCAAATCCACGAACTCCCCTTTCGGCGTAAAGACGTACCCCCGGTCGTCAAAGCCCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCa       <  1:301700/235‑1 (MQ=255)                                                                                                                                                                                                                cccGCAGGCAAATCCACGACATCACCTTTCGGCGTAAAGCCGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCAt  <  1:544627/236‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                               GAACGCCCACGGCTGGTTGTGACGTAACCGAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAAC‑GTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCAT  >  NC_000913/2912183‑2912625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑