Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,689,996	A→C	L89* (TTA→TGA)	bcsZ ←	endo‑1,4‑D‑glucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,689,996	0	A	C	100.0%	182.4 / NA	55	L89* (TTA→TGA)	bcsZ	endo‑1,4‑D‑glucanase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/27);  total (28/27)

CCCGGT‑AACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTAAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCAGGTACAGGCTGCCTGAACACTAAAGGCA  >  NC_000913/3689765‑3690217                                                                                                                                                                                                                                         |                                                                                                                                                                                                                               gccGGTAAACAGCGTGGACCCCGGCCCAGCCACCGTCACCACTCCCCCCCGCGCGATCCGTTTAAGCACCGCCCTGCCGATGTCGGTATGACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAg                                                                                                                                                                                                                             <  2:450992/235‑1 (MQ=255)    ggT‑AAGAAACTGGACCCCAGACCAGGCACTCTCACTACTCCCGCCCTCGCGATACGTTTGAGCAACGCGCTGCGTATGTCGGTATAACGCTGCTCTTTCCACAACCGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGGAcc                                                                                                                                                                                                                         <  2:674208/236‑1 (MQ=255)      gtaccacacgggACCCCAGCCCGGGCCCCGTCACCACTTCCCCCCGCCAGATCCGTTTTAGCAACGCGCTGCCGAGGTCGGTATAACGCTGCTCTTTCCACAACCGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAAcc                                                                                                                                                                                                                         <  2:739828/225‑1 (MQ=255)           accttgaaacccaacccAGGCCCGGTACCCACTTCCTCCCGCCTATTACGTTTAGACCGCCCGGCGCCGTAGTCGGTAAACCCCTGCCCTTCCCCCAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAg                                                                                                                                                                                                                    <  2:499209/228‑1 (MQ=255)           aacgTGGACCCCGGCCCAGGCACCGTCACCCCTTCCTCCCGCGCGATCCGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAACCGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAg                                                                                                                                                                                                                    <  2:112313/235‑1 (MQ=255)               tGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGc                                                                                                                                                                                                                   >  1:623261/1‑232 (MQ=255)                        gcccagGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGACCGAGATTGTTCTg                                                                                                                                                                                                       >  1:165564/1‑235 (MQ=255)                             ggCCCCTTCACCAGTTCCCCCCCGGCGTACCGTTTTAGCCACGCCTTGCCGAGTTCGGTATAACCCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCa                                                                                                                                                                                                 <  2:509429/236‑1 (MQ=255)                                       cgttttCCTCCCGCGCAATACGTTTTAGCAACGGCCTGCCGATGCCGGTATACCGCTGCTCTTTCCACAAGCGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCCCCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAata                                                                                                                                                                                       <  1:450785/232‑1 (MQ=255)                                          cTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAAtatta                                                                                                                                                                                    >  1:651601/1‑236 (MQ=255)                                            tcctccCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCCAGAACCCCGAGCGAGATTGTTCTTGGTGCAGTCGAGAATATTATc                                                                                                                                                                                  >  2:57156/1‑236 (MQ=255)                                              ctccCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGGGAAaattattg                                                                                                                                                                                 >  2:95811/1‑233 (MQ=255)                                               cgccGCGAGATCCTTTGTAGCAACGCGCTGCCGATGTCGGTAAAACGCTGCTCTTTCCCAAAACGCCCCGCCGCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGaa                                                                                                                                                                               <  2:651601/234‑1 (MQ=255)                                                     gcgATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGc                                                                                                                                                                         >  2:730124/1‑236 (MQ=255)                                                      agATACTTTTTAGCAACGCGCTCCGAATGTGGATACACCGCTCCTCTTTCCACAGACGCCCCGCCTACAGCAACGACCAGGCCATCCAGACATCCCCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCa                                                                                                                                                                        <  2:467043/235‑1 (MQ=255)                                                          aCGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGt                                                                                                                                                                    <  1:730124/236‑1 (MQ=255)                                                                      cgcgCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTTTGCGTCCAGTCGAGAATATTATCGAAAGTTGCCAGGACGTTAgccgcc                                                                                                                                                         >  2:329607/1‑235 (MQ=255)                                                                                  tgcgtaaaaccGCTCCTCTTCCAAAAACCGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACa                                                                                                                                            <  2:165564/226‑1 (MQ=255)                                                                                       tataACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGCTTGTTCTGCGTCCAGTCTGGAATATTATCGAAAACTGCCCGGTCGTTAGACGCCACGGCCGGGAAAATGcc                                                                                                                                        >  2:651463/1‑235 (MQ=255)                                                                                       tataACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTa                                                                                                                                                               >  1:340009/1‑212 (MQ=255)                                                                                       tataACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTa                                                                                                                                                               <  2:340009/212‑1 (MQ=255)                                                                                       taaaaCGCTGCTTTTTCCACAAACCCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCCCTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGACCCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCg                                                                                                                                       <  1:57156/233‑1 (MQ=255)                                                                                               tgctCTTTCCACAACCGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGACCCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTg                                                                                                                               <  1:334346/236‑1 (MQ=255)                                                                                               tgctCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACCGTCTTTTCAAGAACCCTGAGAGAGATTGTTCTGCGTCCAGCCGAGAAAATTTACGAAAGCTGCACGGTCGTTAGCCGCACTGGCAGAGAGCAtcacgaaacct                                                                                                                                 >  2:222151/1‑224 (MQ=255)                                                                                               tcctgttcccAAAACCGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCCCTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGACCCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTg                                                                                                                               <  2:369778/228‑1 (MQ=255)                                                                                                 ctctTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAATCCTGAGCGAGATTGTTCTGCGTCCCGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCGGCGAGGGCAGAGAACATGCCCTaaactt                                                                                                                                >  2:334346/1‑229 (MQ=255)                                                                                                 ctctTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGCGAACATGCCGTAAATTTgt                                                                                                                              >  2:274721/1‑234 (MQ=255)                                                                                                                    gCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑ttt                                                                                                          >  1:622648/1‑235 (MQ=255)                                                                                                                                gACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTa                                                                                                                                                               >  2:579875/1‑171 (MQ=255)                                                                                                                                gACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTa                                                                                                                                                               <  1:579875/171‑1 (MQ=255)                                                                                                                                gACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCt                                                                                             <  1:95811/236‑1 (MQ=255)                                                                                                                                          tCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGGGGTGATTTTTGCGC‑CCTCGCTGGGGTcagta                                                                                   >  2:623181/1‑232 (MQ=255)                                                                                                                                                   cACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGACCCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGAcgcg                                                                              <  1:274721/232‑1 (MQ=255)                                                                                                                                                    aCCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGcgcgcg                                                                                                  <  2:254283/211‑1 (MQ=255)                                                                                                                                                    aCCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGcgcgcg                                                                                                  >  1:254283/1‑211 (MQ=255)                                                                                                                                                    aCCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGcc                                                                            >  1:516921/1‑233 (MQ=255)                                                                                                                                                    aCCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGGGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGcc                                                                            >  1:352984/1‑233 (MQ=255)                                                                                                                                                        tCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGcc                                                                            >  1:757822/1‑229 (MQ=255)                                                                                                                                                         cGGAAGCCGAATTGCTGTCCAGCCCTTCCCACTTACTGTTCTTTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGACCCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCctga                                                                    <  1:197130/236‑1 (MQ=255)                                                                                                                                                              gCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCCTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGAt                                                               >  2:491403/1‑236 (MQ=255)                                                                                                                                                                cGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCa                                                                                                                                                                        >  1:537322/1‑130 (MQ=255)                                                                                                                                                                cGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCa                                                                                                                                                                        <  2:537322/130‑1 (MQ=255)                                                                                                                                                                   atggCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAAt                                                          <  1:623181/233‑1 (MQ=255)                                                                                                                                                                         tcccAGCCCTTCCCTCTTACTGTTCCTTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGACCCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCtttt                                                    <  1:329607/234‑1 (MQ=255)                                                                                                                                                                                    cccACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCCGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTcc                                         >  1:615609/1‑236 (MQ=255)                                                                                                                                                                                    cccACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTcc                                         >  1:56101/1‑236 (MQ=255)                                                                                                                                                                                             tGTTCTCTTGCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCaggcaggc                                <  2:757822/236‑1 (MQ=255)                                                                                                                                                                                             tGTTCCGTTTCTGGCCCCGACGCCAGGCGGGCAACCGTGCTTGTCAAGTCCCCTGGGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCaggcaggc                                <  2:622648/236‑1 (MQ=255)                                                                                                                                                                                               ttCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATTACGCGCCCTTCCTGCCTGATGTAATCCTTTTTAAATTGCTCCCCGGCAGGACa                              >  2:529497/1‑236 (MQ=255)                                                                                                                                                                                               ttCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCa                              >  2:155772/1‑236 (MQ=255)                                                                                                                                                                                                 ctctTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTa                                                                                                                                                               <  1:334480/106‑1 (MQ=255)                                                                                                                                                                                                 ctctTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTa                                                                                                                                                               >  2:334480/1‑106 (MQ=255)                                                                                                                                                                                                 ctctTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCCTTTTAAACTGCTCCCAGGCAGGCCAgg                            >  1:726933/1‑236 (MQ=255)                                                                                                                                                                                                             ccACAGCCAGGCGGGCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCAGGTACAGGCTGCCt                <  1:155772/236‑1 (MQ=255)                                                                                                                                                                                                                           gCAAACGTTCTTTTCAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCAGGTACAGGCTGCCCGACCACTAAAGGca  >  2:166630/1‑236 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                               CCCGGT‑AACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACAAACGCCCCGCCTCCAGCAACGACCAGGCCATCCAGACATCACCATCGGAGGCCGAATTGCTGTCCAGCACTTCCCACTTACTGTTCTCTTTCTTGCCCCACAGCCAGGCGGGCAAACGTTCTTTTAAAGAACCCTGAGCGAGATTGTTCTGCGTCCAGTCGAGAATATTATCGAAAGCTGCACGGTCGTTAGCCGCCAGGGCAGAGAACATGCCGTAACTTTGCCCTTCGGAGGTGGTGA‑TTTTGCGCGCGTCGCTGGGGTCGATGACGCGCCCTTCCTGACTGATGTAATCCTTTTTAAACTGCTCCCAGGCAGGCCAGGTACAGGCTGCCTGAACACTAAAGGCA  >  NC_000913/3689765‑3690217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑