Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	95,418	T→G	S257A (TCG→GCG)	murF →	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	95,418	0	T	G	100.0%	198.1 / NA	58	S257A (TCG→GCG)	murF	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/28);  total (30/28)

CGCTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCT  >  NC_000913/95185‑95646                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                     cgcTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACcgcg                                                                                                                                                                                                                                    >  1:136526/1‑236 (MQ=255) cgcTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACcgcg                                                                                                                                                                                                                                    >  1:165253/1‑236 (MQ=255)   tttgctaacaccCTGGCAGGGGCCCTTCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTAAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                  <  2:263554/230‑1 (MQ=255)   cTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                  <  2:98955/236‑1 (MQ=255)   cTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                  <  2:165253/236‑1 (MQ=255)      gTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGGGAGGCGTTTCTCACCCAATG‑CCGCCAACAGCCATTTCACCGCCACCAATATCCATTGGACCGCGCACgg                                                                                                                                                                                                                               >  1:432843/1‑236 (MQ=255)        caccaacCTGGCAGCGGCGCATCTGGAAGGTTTTGGCCCCTTTGCGGGTGTCGCGAAAGCGAAAGGTAAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTa                                                                                                                                                                                                                             <  2:608017/233‑1 (MQ=255)               gtGGCAGCGGCGCATCTGGAAGGTTTGGGCGCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGAAAAACGGTATCGCCATTATGACCGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAAtt                                                                                                                                                                                                                      <  2:324975/235‑1 (MQ=255)               atGGAAGGGGCACATTGGGAAGGTTTGGGCTCGCTTGCGGGGGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAAtt                                                                                                                                                                                                                      <  2:123696/235‑1 (MQ=255)                 ggCAGCGGCGCATCTGGAATGTTTTGGCTCGCTTGCTGGTGTCGCGAGAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTa                                                                                                                                                                                                                    <  2:136526/236‑1 (MQ=255)                      cggcgCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTTACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTa                                                                                                                                                                                                               >  1:184401/1‑236 (MQ=255)                          gcATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGGGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTa                                                                                                                                                                                                               <  1:750611/232‑1 (MQ=255)                               tgaagggTTTGGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCa                                                                                                                                                                                                      <  2:292404/231‑1 (MQ=255)                                 gAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACa                                                                                                                                                                                                             >  2:128852/1‑227 (MQ=255)                                 gAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACa                                                                                                                                                                                                             <  1:128852/227‑1 (MQ=255)                                       tttGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTa                                                                                                                                                                                               >  1:141767/1‑235 (MQ=255)                                                                 agcgaaagGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgg                                                                                                                                                                    <  2:207077/236‑1 (MQ=255)                                                                   cgaaagGTAAACTCTGTGGCTGCCTACCGGAAACCGGTATCGCCATTAGGAACGCCGACAACAACGACGGGCTGAACTGGCAGAGCGAAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgggg                                                                                                                                                                  <  2:153454/236‑1 (MQ=255)                                                                             cgtCTTTAGCGGCCTGCCGGAAACCGGTACCCCACTTAGGAACCCCGACAACAGCGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACaa                                                                                                                                                          <  2:400854/232‑1 (MQ=255)                                                                             aaTCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgggg                                                                                                                                                                  <  1:190612/226‑1 (MQ=255)                                                                                         ccTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCCCAATATTGCGaa                                                                                                                                                 >  1:698922/1‑231 (MQ=255)                                                                                         ccTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGaa                                                                                                                                                 >  1:630422/1‑231 (MQ=255)                                                                                                       gTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGTGCTGGCAGCCgtcgg                                                                                                                               >  2:47688/1‑231 (MQ=255)                                                                                                       gTATCCCCATTAGGACCGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTgcg                                                                                                                              <  1:756028/236‑1 (MQ=255)                                                                                                           cacattgaTGAACGCCGACAAGAACGACTGGCTGAACTGGCAGACCGTAATTGGCTCACGCAAGGTGTGGCTTTTCTCACCCAATG‑CCGCCAACAGCGATTCCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTgcg                                                                                                                              <  2:402810/225‑1 (MQ=255)                                                                                                                ttATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATg                                                                                                                     >  1:164418/1‑236 (MQ=255)                                                                                                                  aTGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCCCGGTACGGAATTTACCCTACCAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGCCACAATATTGGGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTc                                                                                                                   >  2:665878/1‑236 (MQ=255)                                                                                                                  aTGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAAAATTGCGAATGCGCTGGCAGCCCCTGGGCTCTCCATGTc                                                                                                                   >  2:621213/1‑236 (MQ=255)                                                                                                                        gCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTggg                                                                                                             >  1:534344/1‑236 (MQ=255)                                                                                                                            acaacaACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                  >  1:248127/1‑115 (MQ=255)                                                                                                                            acaacaACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa                                                                                                                                                                                                                                  <  2:248127/115‑1 (MQ=255)                                                                                                                                   cGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGGGCAACGCt                                                                                                    >  1:67809/1‑234 (MQ=255)                                                                                                                                         gCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGc                                                                                                                                        <  2:42701/192‑1 (MQ=255)                                                                                                                                         gCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGc                                                                                                                                        >  1:42701/1‑192 (MQ=255)                                                                                                                                          ttGAACTGGCTAGCCGTATTTGGCTAACGCAAAGTTTGCCTTTGCCCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATCTCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTAt                                                                                           <  2:184401/235‑1 (MQ=255)                                                                                                                                          ctaaccTGGCAGGGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCCCACCCAATG‑CCCCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTAt                                                                                           <  2:324586/231‑1 (MQ=255)                                                                                                                                                        cGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCGCTCCATGTCCGTGGGCGCCACGCTTGATGCTATCAAAGCggctcggg                                                                             >  2:418635/1‑230 (MQ=255)                                                                                                                                                            aTTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCa                                                                           <  1:47688/234‑1 (MQ=255)                                                                                                                                                                    aCGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTg                                                                     >  1:424364/1‑232 (MQ=255)                                                                                                                                                                              tgGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAg                                                        >  2:434445/1‑235 (MQ=255)                                                                                                                                                                              tgGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCGTTCAAACCGGGGCTGGCAATCCTGAAACATGTTCCAg                                                        >  2:302774/1‑235 (MQ=255)                                                                                                                                                                                gCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCCCCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGc                                                      <  1:576309/235‑1 (MQ=255)                                                                                                                                                                                      ctcACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTgcg                                                                                                                              <  2:755667/157‑1 (MQ=255)                                                                                                                                                                                      ctcACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTgcg                                                                                                                              >  1:755667/1‑157 (MQ=255)                                                                                                                                                                                      ctcACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTTGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTg                                                >  1:306850/1‑235 (MQ=255)                                                                                                                                                                                      ctcACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTg                                                >  1:237969/1‑235 (MQ=255)                                                                                                                                                                                        cACCCAAAGCCCGCCAACAGCATTTTCACCGCCACCAATATCCATGTGGCCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGtt                                              <  2:202114/236‑1 (MQ=255)                                                                                                                                                                                           ccAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTg                                                >  2:600151/1‑230 (MQ=255)                                                                                                                                                                                           ccAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTg                                                <  1:600151/230‑1 (MQ=255)                                                                                                                                                                                              aTG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGTGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCTCGTCCGTCTGTTTCCCAt                                        >  2:100832/1‑235 (MQ=255)                                                                                                                                                                                               tG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATcc                                      >  1:35517/1‑236 (MQ=255)                                                                                                                                                                                               tG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATcc                                      >  1:531123/1‑236 (MQ=255)                                                                                                                                                                                                   cgccAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAAc                                   <  1:434445/236‑1 (MQ=255)                                                                                                                                                                                                     ccAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTg                                 >  1:545415/1‑236 (MQ=255)                                                                                                                                                                                                                         accaATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTgctgct             >  2:346035/1‑236 (MQ=255)                                                                                                                                                                                                                             attgccATGTGACCGGACACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTcgac         <  2:630422/232‑1 (MQ=255)                                                                                                                                                                                                                              tacccATGTGACCGGGCAGGGTACGGAATTTCCCCTACAACCCCCAACCGGTAGCGTGGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTcgacg        <  2:534344/233‑1 (MQ=255)                                                                                                                                                                                                                                    tgtgACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCt  >  1:741849/1‑236 (MQ=255)                                                                                                                                                                                                                                    tgtgACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCt  >  1:196187/1‑236 (MQ=255)                                                                                                                                                                                                                                     gtgACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCt  >  1:466676/1‑235 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                     CGCTGGTCAACAACCTGGCAGCGGCGCATCTGGAAGGTTTTGGCTCGCTTGCGGGTGTCGCGAAAGCGAAAGGTGAAATCTTTAGCGGCCTGCCGGAAAACGGTATCGCCATTATGAACGCCGACAACAACGACTGGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATG‑CCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCT  >  NC_000913/95185‑95646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑