Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,134,118	A→C	Q106P (CAG→CCG)	flgF →	flagellar component of cell‑proximal portion of basal‑body rod

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,134,118	0	A	C	100.0%	248.3 / NA	69	Q106P (CAG→CCG)	flgF	flagellar component of cell‑proximal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (37/32);  total (37/32)

TGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCAGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAG  >  NC_000913/1133885‑1134346                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      tGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGGATACGCGGAATGGCAGCATTCCgg                                                                                                                                                                                                                                     >  1:199257/1‑236 (MQ=255)                 ttgcgcgagTAGGTGAATGCTTGACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCGCGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCa                                                                                                                                                                                                                     <  2:643967/233‑1 (MQ=255)                    cgcgcgCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCCGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGGATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACt                                                                                                                                                                                                                  >  1:254047/1‑236 (MQ=255)                    cgcgcgCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACt                                                                                                                                                                                                                  <  1:570051/236‑1 (MQ=255)                      cgcgcAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGa                                                                                                                                                                                                                <  2:65548/236‑1 (MQ=255)                      cgcgcAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTGCTCTGCCCACGCGCACGGTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGa                                                                                                                                                                                                                <  1:676967/236‑1 (MQ=255)                                  tGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCGCGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGc                                                                                                                                                                                                    <  1:267046/236‑1 (MQ=255)                                     tttACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCCCGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATc                                                                                                                                                                                                 <  1:645414/236‑1 (MQ=255)                                     tttACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACGCCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATc                                                                                                                                                                                                 <  2:157489/236‑1 (MQ=255)                                     tttACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATc                                                                                                                                                                                                 >  1:107159/1‑236 (MQ=255)                                     tttACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATc                                                                                                                                                                                                 <  1:463927/236‑1 (MQ=255)                                     tttACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATc                                                                                                                                                                                                 >  1:368891/1‑236 (MQ=255)                                              ggTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGGATAAGCGGAAGTGCAGCCTTCCCGTTGATCCCACCGGGCAACAGACAATCCAGGGGCGACCggcggtgc                                                                                                                                                                                        >  2:393179/1‑230 (MQ=255)                                                ttcaaGTGGAAGGGCGTGCCCGGCCCAAGCGCAAGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGAGATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAgg                                                                                                                                                                                       <  2:687846/231‑1 (MQ=255)                                                   cAGTGGAAGGGCTTTCTCCGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAAGGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAgggg                                                                                                                                                                                                     <  1:335198/218‑1 (MQ=255)                                                          aGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGc                                                                                                                                                                            >  1:107826/1‑236 (MQ=255)                                                               tttCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGGTGATCCCACCGGGCAACTGACAATTCAGGGGCCTACGGTGAAAGGCGAGGCTGGGCCAAtt                                                                                                                                                                       >  2:67648/1‑236 (MQ=255)                                                                  ctctGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGc                                                                                                                                                                     <  1:81096/235‑1 (MQ=255)                                                                       cccACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCGGCATTCCGGTTGATCCCACCGGGCAACTGACAATTACGGGGCATACGGTGATAGGCGAGGCTGGGCGAATTGCTGTGcc                                                                                                                                                               >  2:30272/1‑236 (MQ=255)                                                                         cACGCCCACGTTGGTC‑CGGCCGCACACGGCGGGCGCAGATATGACGCACGGCAAAGTGGATTACCCCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCgg                                                                                                                                                             <  2:634437/236‑1 (MQ=255)                                                                           cgcgCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTTCCGGaa                                                                                                                                                           >  2:143489/1‑236 (MQ=255)                                                                           cgcgCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCAGGTGATAGGCTAGGCTGGGCCAAATGCTGTGCCGGac                                                                                                                                                           >  2:276182/1‑235 (MQ=255)                                                                            gcgcACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGGGCCGGAAg                                                                                                                                                          >  1:472456/1‑236 (MQ=255)                                                                             cgcACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAgg                                                                                                                                                                                                       <  2:671582/190‑1 (MQ=255)                                                                             cgcACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAgg                                                                                                                                                                                                       >  1:671582/1‑190 (MQ=255)                                                                                 tgTTGGTCACTGCGTCA‑ACGCCGGGCGCAGATATGACGCCAGGCAAAATGGATTACGCCGCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCg                                                                                                                                                     <  2:107159/235‑1 (MQ=255)                                                                                          cGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTg                                                                                                                                                                                            <  1:672244/188‑1 (MQ=255)                                                                                          cGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTg                                                                                                                                                                                            >  2:672244/1‑188 (MQ=255)                                                                                                cA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATtgct                                                                                                                                      >  1:724164/1‑236 (MQ=255)                                                                                                  tttgCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATtgctg                                                                                                                                     >  1:463907/4‑236 (MQ=255)                                                                                                     gCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCg                                                                                                                                  <  2:689126/236‑1 (MQ=255)                                                                                                       cGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGAt                                                                                                                                <  2:472456/236‑1 (MQ=255)                                                                                                        gggCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGc                                                                                                                                                                                      <  2:252959/181‑1 (MQ=255)                                                                                                        gggCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGc                                                                                                                                                                                      >  1:252959/1‑181 (MQ=255)                                                                                                         ggCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCCGGGGCATCCGGTGATAGGCGAGGCCGGGCCACTTGCTGTTCAGGTAGGGGCGGGAATCACTATTGCTGCCGAttg                                                                                                                              >  2:595216/1‑234 (MQ=255)                                                                                                              aGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCGGGGCCAATTGCTGTGCCGGAATGGGGGGAAATCACCACTGATGCCGATGGcaca                                                                                                                          >  2:542129/1‑235 (MQ=255)                                                                                                                    gACGCCCGGCAAAGTGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑tctc                                                                                                                    <  1:321062/234‑1 (MQ=255)                                                                                                                    gACGCCCGGCAAAAGGGATTACCCCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCg                                                                                                                   <  1:30272/235‑1 (MQ=255)                                                                                                                     aCGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGgc                                                                                                                 >  1:113198/1‑236 (MQ=255)                                                                                                                        cccgcaaaaaTGGATTCCCCCTCGCGCCCCGTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGgcg                                                                                                                <  2:199257/228‑1 (MQ=255)                                                                                                                          cGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTc                                                                                                             >  1:606319/1‑235 (MQ=255)                                                                                                                           ggCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCGGTGCCGGCAGGGGCGGAAATCACTATTGCTGCCGATGGCTCAAGTCT‑GGCGCTCaa                                                                                                           >  2:50787/1‑236 (MQ=255)                                                                                                                            gCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAAt                                                                                                          <  1:669129/236‑1 (MQ=255)                                                                                                                                       tACACCTCGCGCCGGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGAt                                                                                                 <  2:254047/234‑1 (MQ=255)                                                                                                                                              cgcgCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGGAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCCCAATCCGGCCGATCCgggcaaa                                                                                        >  2:472158/1‑231 (MQ=255)                                                                                                                                               gcgcCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGGGGTAGGCGAGGCTGGGCCAATTGCTGTGCCGGACGGGGCGGAAATCACTATTGCTGCCGCTGGCACAA‑ATTCGGGCGTCAATCCCGGGGATCCTGCa                                                                                           >  2:6177/1‑232 (MQ=255)                                                                                                                                                cgcCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCCGTGCCGGCAGGGGCGGAAATCACCATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCCGGCCATACGGCCAATAc                                                                                      >  2:686793/1‑236 (MQ=255)                                                                                                                                                               gcgTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAgg                                                                       >  1:53487/1‑236 (MQ=255)                                                                                                                                                               gcgTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAgg                                                                       <  2:113198/236‑1 (MQ=255)                                                                                                                                                               gcgTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAgg                                                                       <  2:463907/236‑1 (MQ=255)                                                                                                                                                                      agcagGAAGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAgg                                                                       <  1:311984/229‑1 (MQ=255)                                                                                                                                                                        cagGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGgcg                                                                                                                <  1:448918/186‑1 (MQ=255)                                                                                                                                                                             tggctggctggcCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGGGCACGTAGGGCGGCTTaaa                                                             >  2:311984/1‑232 (MQ=255)                                                                                                                                                                               gctggctggcCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGGTTGCACCA‑CCTCGGCGCTCAATc                                                                                                         >  2:448918/1‑186 (MQ=255)                                                                                                                                                                                  ggctggcCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATcc                                                                                                                                                                                                <  2:333065/97‑1 (MQ=255)                                                                                                                                                                                  ggctggcCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATcc                                                                                                                                                                                                >  1:333065/1‑97 (MQ=255)                                                                                                                                                                                     tggcCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGcc                                                 >  1:713694/1‑236 (MQ=255)                                                                                                                                                                                         cGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACgg                                             >  1:405790/1‑236 (MQ=255)                                                                                                                                                                                          gTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGGCTTAAAATGGTGAAAAGCACgtg                                            >  2:586413/1‑234 (MQ=255)                                                                                                                                                                                           tGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACggg                                            <  1:143489/235‑1 (MQ=255)                                                                                                                                                                                           tGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGc                                           >  1:648070/1‑236 (MQ=255)                                                                                                                                                                                             cAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAg                                         >  1:284045/1‑236 (MQ=255)                                                                                                                                                                                                      gACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCa                                <  1:388952/236‑1 (MQ=255)                                                                                                                                                                                                            aGCGAAGGGTATACGCGTAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAgaccgg                          >  1:14903/1‑231 (MQ=255)                                                                                                                                                                                                                   ggTATACGCGTAATGGAAGCATTCCGGTTGATCCCGCCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTgacga                    <  1:256543/235‑1 (MQ=255)                                                                                                                                                                                                                             tAATGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTc         >  1:44694/1‑236 (MQ=255)                                                                                                                                                                                                                              aaTGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACAGGCAGCGAAGGTCAGCCCGGGGGTGACGGCAttttcat        >  2:473835/1‑233 (MQ=255)                                                                                                                                                                                                                               aTGGCAGCATTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGt       >  2:339589/1‑236 (MQ=255)                                                                                                                                                                                                                                    agcaTTCCGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAg  <  2:648070/236‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      TGCCTCAACGCCCGGTTTTCGCGCGCAGTTGAATGCTTTACGCGCGGTGCCAGTGGAAGGGCTTTCTCTGCCCACGCGCACGTTGGTCACGGCGTCA‑ACGCCGGGCGCAGATATGACGCCCGGCAAAATGGATTACACCTCGCGCCCGCTGGACGTCGCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACGGCAGCGAAGGGTATACGCGTAATGGCAGCATTCAGGTTGATCCCACCGGGCAACTGACAATTCAGGGGCATCCGGTGATAGGCGAGGCTGGGCCAATTGCTGTGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAA‑TCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAG  >  NC_000913/1133885‑1134346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑