Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,8810C.95.0% 878.9 / 42.8 203intergenic (+235/‑131)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (8/2);  new base . (87/103);  total (96/107)
Fisher's exact test for biased strand distribution p-value = 4.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

TAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATAT  >  NC_000913/2462782‑2462975
                                                                                                   |                                                                                              
tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTc                                                                                                >  1:4280842/1‑100 (MQ=255)
tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCGCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTc                                                                                                >  2:878122/1‑100 (MQ=255)
  aGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccc                                                                                              >  1:2092776/1‑100 (MQ=255)
       tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                                         >  2:3457037/1‑100 (MQ=255)
       tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                                         <  1:4252543/100‑1 (MQ=255)
       tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACCTTTCTGCATTTCCAGGATACTcccccccc                                                                                         <  2:260633/100‑1 (MQ=255)
        aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccct                                                                                        >  1:560654/1‑99 (MQ=255)
         tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctg                                                                                       <  1:1594036/100‑3 (MQ=255)
         tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctg                                                                                       <  2:1267981/100‑3 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     <  1:3091952/100‑5 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     >  1:536255/1‑96 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     >  2:2859732/1‑96 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     >  2:5236651/1‑96 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     <  1:1395599/100‑5 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctggc                                                                                     >  1:3724930/1‑96 (MQ=255)
           cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGCCCTTCTCAAAGACTTTACTTTTCTGCATTTTCAGGATACTcccccccctctc                                                                                     >  2:1144179/1‑96 (MQ=255)
              aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTAt                                                                                 >  1:1821869/1‑100 (MQ=255)
               gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTAtt                                                                                >  1:3212295/1‑100 (MQ=255)
                aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtg                                                                               <  1:2386992/100‑1 (MQ=255)
                 cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgt                                                                              >  2:2455702/1‑100 (MQ=255)
                 cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgt                                                                              >  1:5242246/1‑100 (MQ=255)
                 cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACGCCCCCCCCTGGCTATtgt                                                                              <  1:1039930/100‑1 (MQ=255)
                  aaaCCTGGAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                             <  2:2286786/100‑1 (MQ=255)
                  aaaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                             <  2:2905191/100‑1 (MQ=255)
                   aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgc                                                                            >  1:116488/1‑100 (MQ=255)
                      cTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCt                                                                         >  2:3253942/1‑100 (MQ=255)
                       tGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTc                                                                        >  1:2860895/1‑100 (MQ=255)
                       tGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTc                                                                        <  1:2278664/100‑1 (MQ=255)
                         aaGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCAt                                                                      <  1:3489411/100‑1 (MQ=255)
                          aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATa                                                                     <  2:1662140/100‑1 (MQ=255)
                           gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATac                                                                    >  2:817893/1‑100 (MQ=255)
                           gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATac                                                                    <  2:4467968/100‑1 (MQ=255)
                           gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGCTTCCTTTTCTGCATTTCCAGAATCCTACCCCCCCTGCCGATTGTTCGCTCAtcc                                                                    >  1:4761161/1‑98 (MQ=255)
                            ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATaca                                                                   >  1:460235/1‑100 (MQ=255)
                              aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                 >  1:225057/1‑100 (MQ=255)
                              aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                 <  2:1829015/100‑1 (MQ=255)
                                 ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCaa                                                              >  1:3564470/1‑100 (MQ=255)
                                 ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCaa                                                              >  2:4035154/1‑100 (MQ=255)
                                   cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAt                                                            <  2:2154789/100‑1 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAt                                                            >  2:1732027/1‑100 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAtt                                                           >  1:3866803/1‑100 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAtt                                                           <  2:2833716/100‑1 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAtt                                                           <  1:482686/100‑1 (MQ=255)
                                    atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGATTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAtt                                                           >  2:738571/1‑100 (MQ=255)
                                     tatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                          >  2:70795/1‑100 (MQ=255)
                                       ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTaaa                                                        >  1:3593664/1‑100 (MQ=255)
                                       ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTaaa                                                        <  1:3669984/100‑1 (MQ=255)
                                        tttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAg                                                       <  1:828678/100‑1 (MQ=255)
                                           cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATa                                                    >  2:515861/1‑100 (MQ=255)
                                           cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATa                                                    >  2:4128299/1‑100 (MQ=255)
                                           cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATa                                                    >  1:3391401/1‑100 (MQ=255)
                                           cAGGCGTTCTGCTCGTCCATCTCAAAGAGTTTAATTTTCTGCATTTCCAGGATACAAAACAACCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATa                                                    <  2:4288115/100‑1 (MQ=255)
                                             ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                  >  1:3816957/1‑100 (MQ=255)
                                               cGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                <  2:3747657/100‑1 (MQ=255)
                                                gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTc                                               >  2:1968658/1‑100 (MQ=255)
                                                     gCTTGTCCTTCACAAACAGTTAACTTTTCTAAATTAGCAGGATACTCACCCACCTGGCTATTATCCTCCAATACACTCAAATTAAATATAGGTTCtaaat                                          <  2:210780/100‑1 (MQ=255)
                                                     gCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                          >  2:385734/1‑100 (MQ=255)
                                                      cTCGTCCTTCTCAAAGAGTTTACTTTTTTTTATTTCCAGGATACTCCCCCCCCCGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                         <  2:1230424/100‑1 (MQ=255)
                                                      cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                         >  2:4783538/1‑100 (MQ=255)
                                                      cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                         <  1:5245508/100‑1 (MQ=255)
                                                        cGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataaa                                       <  2:854449/100‑1 (MQ=255)
                                                           ccTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGa                                    >  1:362134/1‑100 (MQ=255)
                                                              tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg                                 >  2:4999865/1‑100 (MQ=255)
                                                              tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg                                 >  2:5274190/1‑100 (MQ=255)
                                                                tcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtt                               <  2:3182627/100‑1 (MQ=255)
                                                                 cAAAGAGTTTACTTGTCGGCATGTCCAGGATACGCCCCCCCCGGGCTATTGGGCGCTCATACACTCAAATTAAAGAGAGGTTCTAAATAATGGATCGttt                              >  2:3025571/1‑100 (MQ=255)
                                                                  aaaGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttt                             >  1:2978439/1‑100 (MQ=255)
                                                                   aaGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttttt                            <  2:3338207/100‑1 (MQ=255)
                                                                    agagTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttttt                           <  1:5427045/100‑1 (MQ=255)
                                                                       gTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                        <  2:427735/100‑1 (MQ=255)
                                                                       gTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                        <  1:2709156/100‑1 (MQ=255)
                                                                        tttACTTTTCTGCATTTCCAGGATCCTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                       <  1:4166560/100‑1 (MQ=255)
                                                                         ttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      <  1:3437221/100‑1 (MQ=255)
                                                                         ttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      >  2:297423/1‑100 (MQ=255)
                                                                         ttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      >  1:1712953/1‑100 (MQ=255)
                                                                          tACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      >  1:467654/1‑100 (MQ=255)
                                                                          tACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                      >  1:3183828/1‑100 (MQ=255)
                                                                          tACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAGATAAATGAGCGTTTTTTGATAGt                     >  2:1851234/1‑100 (MQ=255)
                                                                          tACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                     >  1:2958228/1‑100 (MQ=255)
                                                                           aCTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATaaga                    >  2:511289/1‑97 (MQ=255)
                                                                           aCTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTc                    <  1:2031678/100‑1 (MQ=255)
                                                                           aCTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTc                    <  2:624439/100‑1 (MQ=255)
                                                                            cTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCt                   >  2:4932599/1‑100 (MQ=255)
                                                                            cTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCt                   <  1:1282686/100‑1 (MQ=255)
                                                                             ttttCTGCATTTCCAGGATACT‑‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAt                 >  2:5436079/1‑100 (MQ=255)
                                                                                tCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt               >  2:378748/1‑100 (MQ=255)
                                                                                tCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt               <  2:2387153/100‑1 (MQ=255)
                                                                                tCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt               >  1:906725/1‑100 (MQ=255)
                                                                                tCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt               >  1:3890991/1‑100 (MQ=255)
                                                                                 cTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc              >  1:1943695/1‑100 (MQ=255)
                                                                                 cTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc              >  1:962591/1‑100 (MQ=255)
                                                                                  tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa             >  1:2529301/1‑100 (MQ=255)
                                                                                  tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa             <  2:1073329/100‑1 (MQ=255)
                                                                                   gCATTTCCAGGATACTCCCCCCCCTTTCTAGTGTGCTTTCATACACGCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt            <  2:3918558/100‑1 (MQ=255)
                                                                                   gCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt            >  1:4978224/1‑100 (MQ=255)
                                                                                    cATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAGATAAATGAGCGTTTTTTGATAGTCTATTTCAtt           <  1:1872078/100‑1 (MQ=255)
                                                                                      tttCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg         <  2:3531517/100‑1 (MQ=255)
                                                                                      tttCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAAAAAATGAGCGTTTTTTGATAGTCTATTTCATTAg         >  1:5509363/1‑100 (MQ=255)
                                                                                          cAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTTAAATTAAAGATAGGTTTTAAAAATATGAGCGTTTTTTGATAGTCTATTTCATTAGGTaa     <  2:5366724/100‑1 (MQ=255)
                                                                                            ggATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAata   >  1:5327763/1‑100 (MQ=255)
                                                                                                   |                                                                                              
TAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATAT  >  NC_000913/2462782‑2462975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: