Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,655,917 (A)5→4 intergenic (‑15/+49) yhiD ← / ← hdeB putative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,655,9130A.98.2% 1031.7 / 9.5 224intergenic (‑11/+53)yhiD/hdeBputative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base . (114/106);  total (117/107)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 242 total aligned reads displayed.

GCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAAT  >  NC_000913/3655814‑3656012
                                                                                                   |                                                                                                    
gCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCa                                                                                                      <  1:114916/100‑1 (MQ=255)
gCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCa                                                                                                      <  2:2187754/100‑1 (MQ=255)
   ccTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCaaaa                                                                                                   >  2:1079092/1‑100 (MQ=255)
    cTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCaaaag                                                                                                  <  2:3560237/100‑2 (MQ=255)
    cTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCaaaag                                                                                                  <  2:4433418/100‑2 (MQ=255)
    cTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCaaaag                                                                                                  <  2:2854347/100‑2 (MQ=255)
      ttGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCaaaagga                                                                                                <  2:2100641/100‑4 (MQ=255)
       tGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCaaaaggag                                                                                               <  1:5092076/100‑5 (MQ=255)
        gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGagc                                                                                             <  2:3619000/100‑1 (MQ=255)
           gcgcATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGagcagc                                                                                          <  2:1800968/100‑1 (MQ=255)
           gcgcATTTGCCCTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGagcagc                                                                                          <  1:780055/100‑1 (MQ=255)
            cgcATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAgcagca                                                                                         <  1:2581620/100‑1 (MQ=255)
            cgcATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAgcagca                                                                                         <  2:1648888/100‑1 (MQ=255)
              cATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAg                                                                                       >  2:2291214/1‑100 (MQ=255)
              cATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAg                                                                                       <  1:3586328/100‑1 (MQ=255)
                tttGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAgtt                                                                                     >  2:1974397/1‑98 (MQ=255)
                tttGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGAt                                                                                     <  2:2044887/100‑1 (MQ=255)
                tttGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGAt                                                                                     <  2:3910120/100‑1 (MQ=255)
                 ttGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATg                                                                                    <  2:5354738/100‑1 (MQ=255)
                  tGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATgg                                                                                  <  2:1433272/100‑1 (MQ=255)
                  tGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATgg                                                                                  <  1:4137238/100‑1 (MQ=255)
                   gCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGc                                                                                 <  2:5265733/100‑1 (MQ=255)
                   gCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAAGAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGc                                                                                 <  2:5323980/100‑1 (MQ=255)
                    ccTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCt                                                                                <  1:3621896/100‑1 (MQ=255)
                    ccTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCt                                                                                >  1:4109520/1‑100 (MQ=255)
                      tttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCa                                                                              >  1:3200804/1‑100 (MQ=255)
                      tttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCa                                                                              <  1:5470712/100‑1 (MQ=255)
                      tttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCa                                                                              >  1:3612448/1‑100 (MQ=255)
                       ttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCaa                                                                             >  2:2666324/1‑100 (MQ=255)
                         ccATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACt                                                                           >  1:4272623/1‑100 (MQ=255)
                          cATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACtt                                                                          <  1:101135/100‑1 (MQ=255)
                           aTGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTg                                                                         >  2:3212421/1‑100 (MQ=255)
                             gCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGGTCAACTTGCt                                                                       <  2:5060478/100‑1 (MQ=255)
                              ccAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTa                                                                      >  1:3806002/1‑100 (MQ=255)
                               cAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTAc                                                                     >  2:3356067/1‑100 (MQ=255)
                               cAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTAc                                                                     <  2:2119840/100‑1 (MQ=255)
                               cAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAAATCTGCTGTCACATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTAc                                                                     <  2:1744859/100‑1 (MQ=255)
                                aaTAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAACTGCAAAAGGATCAGAAAGATGGCTCAACTTGCTACt                                                                    >  2:5195669/1‑100 (MQ=255)
                                aaTAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACt                                                                    <  1:5557762/100‑1 (MQ=255)
                                 aTAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCGGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTc                                                                   >  2:1119675/1‑100 (MQ=255)
                                  tAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTcc                                                                  <  2:3521357/100‑1 (MQ=255)
                                    gcgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCtt                                                                <  1:1414063/100‑1 (MQ=255)
                                    gcgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCtt                                                                >  1:2761237/1‑100 (MQ=255)
                                    gcgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCtt                                                                <  1:4487300/100‑1 (MQ=255)
                                      gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGTTGAGCCATCTTGCTGCTCCtttt                                                              <  1:1996009/100‑1 (MQ=25)
                                      gcCACAGGCAATGGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCtttt                                                              >  2:256288/1‑100 (MQ=255)
                                        cacaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTa                                                            >  2:3022069/1‑100 (MQ=255)
                                         acaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTAc                                                           <  1:2161952/100‑1 (MQ=255)
                                         acaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGAAGCAGCAAGATGGCTCAACTTGCTACCCCTTTTTAc                                                           >  2:2829507/1‑100 (MQ=255)
                                          caGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTAc                                                           >  1:1556153/1‑100 (MQ=255)
                                          caGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACt                                                          <  1:1864092/100‑1 (MQ=255)
                                          caGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACt                                                          >  1:2408381/1‑100 (MQ=255)
                                            ggCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTg                                                        >  2:510492/1‑100 (MQ=255)
                                               aaTTGCCGCGAGTATACGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAAGTTGCTACTCCTTTTTACTTGCAc                                                     <  2:4603764/100‑1 (MQ=255)
                                               aaTTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAc                                                     >  2:1517515/1‑100 (MQ=255)
                                                aTTGCCGCGAGTATAAGATGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAcc                                                    <  1:5220112/100‑1 (MQ=255)
                                                aTTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAcc                                                    >  2:2418180/1‑100 (MQ=255)
                                                 ttGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAGATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCt                                                   <  1:5042417/100‑1 (MQ=255)
                                                  tGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTc                                                  <  2:3991928/100‑1 (MQ=255)
                                                     cgcgAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAtt                                               >  1:4623797/1‑100 (MQ=255)
                                                     cgcgAGTATAAGACGTATAATAAATTCTGAAAAAATAAAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAtt                                               <  2:663759/100‑1 (MQ=255)
                                                     cgcgAGTATAAGACGTATAATAAATTAAAATAAAATAAAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAtt                                               <  2:598228/100‑1 (MQ=255)
                                                      gcgAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTa                                              <  1:92969/100‑1 (MQ=255)
                                                          gTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTc                                          >  2:4744124/1‑100 (MQ=255)
                                                          gTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTc                                          >  1:3428653/1‑100 (MQ=255)
                                                            ataAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCgg                                        <  1:4600632/100‑1 (MQ=255)
                                                              aaGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCa                                      >  1:4094995/1‑100 (MQ=255)
                                                              aaGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCa                                      >  2:1210770/1‑100 (MQ=255)
                                                                gACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAg                                    <  2:3771079/100‑1 (MQ=255)
                                                                gACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAg                                    <  1:3749799/100‑1 (MQ=255)
                                                                gACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAg                                    <  2:3431950/100‑1 (MQ=255)
                                                                  cttataATAGATTCTGCTGTCATATAAAAATGCAAAAGGCGCAGTAAGATAGCTCAACTTGCTATTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTc                                  <  2:1586469/98‑1 (MQ=255)
                                                                  cGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTc                                  >  1:1430067/1‑100 (MQ=255)
                                                                  cGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGATCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTc                                  >  2:5020923/1‑100 (MQ=255)
                                                                      taataaATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                              >  2:4972085/1‑100 (MQ=255)
                                                                      taataaATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                              >  1:844068/1‑100 (MQ=255)
                                                                      taataaATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                              >  1:1591805/1‑100 (MQ=255)
                                                                       aataaATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAg                             >  1:1080286/1‑100 (MQ=255)
                                                                       aataaATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAg                             <  1:624828/100‑1 (MQ=255)
                                                                       aataaATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAg                             >  1:2329637/1‑100 (MQ=255)
                                                                        ataaATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGa                            >  2:4493679/1‑100 (MQ=255)
                                                                        ataaATTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGa                            <  2:4709794/100‑1 (MQ=255)
                                                                          aaaTTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATg                          >  2:3876914/1‑100 (MQ=255)
                                                                          aaaTTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATg                          >  2:4382739/1‑100 (MQ=255)
                                                                          aaaTTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATg                          <  2:834935/100‑1 (MQ=255)
                                                                           aaTTCTGCTGTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGc                         <  2:895058/100‑1 (MQ=255)
                                                                                 gctgTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAtt                   <  2:4162318/100‑1 (MQ=255)
                                                                                  ctgTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAttt                  >  1:2882945/1‑100 (MQ=255)
                                                                                    gTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAttttt                >  1:2566859/1‑100 (MQ=255)
                                                                                    gTCATATAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAttttt                >  2:1319032/1‑100 (MQ=255)
                                                                                       atatAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCGTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa             >  1:5375570/1‑100 (MQ=255)
                                                                                       atatAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa             >  2:743344/1‑100 (MQ=255)
                                                                                       atatAAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa             <  2:2934332/100‑1 (MQ=255)
                                                                                        tataAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAg            <  1:2344103/100‑1 (MQ=255)
                                                                                         ataAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAgg           >  1:3152639/1‑100 (MQ=255)
                                                                                         ataAAAATGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAgg           <  1:2088569/100‑1 (MQ=255)
                                                                                            aaaaTGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGtat        >  2:5443506/1‑100 (MQ=255)
                                                                                            aaaaTGCAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGtat        >  1:1506699/1‑100 (MQ=255)
                                                                                               aagtaaaaaGGATTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATAc      >  1:301394/5‑100 (MQ=18)
                                                                                                   aaaaaGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAAt  >  1:3320681/1‑100 (MQ=21)
                                                                                                   |                                                                                                    
GCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAAT  >  NC_000913/3655814‑3656012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: